nu1,nu2,nu3

[pengyan19]

how did i transform the nu1 to real Ne?,i found that nu1<1, and nu2>1,but i found that nu1+nu2=nuA, so in three population, nu1,nu2,nuA,nu3,how did i transform ?

[dportik]

Hello @pengyan19 , The dadi user group contains discussions about transforming parameters, and I would encourage you to double check this information there too.

Real population size = nu * Nref -nu is the inferred unscaled population size

Nref requires an additional calculation involving theta to get the reference population estimate:

Theta = 4 Nref mu * L

• Theta is calculated for every model fit, you will have this value
• mu refers to the mutation rate
• L is the total length of DNA sequence (in bp) that was analyzed in order to obtain our SNP data

So you must first solve for Nref (based on theta), then use Nref value to scale nu.

Example: Theta = 4 Nref mu L 87.08 = Nref 4 0.00000001 289,873 87.08 = Nref * 0.01159492 7,510 = Nref