AptaPLEX bug - Paired-end reads parsing

drivenbyentropy / aptasuite

A full-featured bioinformatics software collection for the comprehensive analysis of aptamers in HT-SELEX experiments.

https://drivenbyentropy.github.io/

GNU General Public License v3.0

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AptaPLEX bug - Paired-end reads parsing #11

Closed PJpb closed 6 years ago

PJpb commented 6 years ago

Hi, Unfortunetely I've got a bug this time as well. I'm using command-line aptasuite-0.5.3 via Windows PowerShell. The files contain real-data derived subsets with 10000 reads each (forward and reverse), quality-filtered, etc. Please find the aptasuite log and the powershell output. log_2018-02-16_11-28-10.txt errorlog160218.txt

drivenbyentropy commented 6 years ago

You have been busy :)

Could you please attach your configuration file as well? I strongly suspect this to be due to a misconfiguration (file path in windows must be escaped in a specific way) or corrupt fastq files as it is working without issues in my setup.

If you are allowed to share the sequencing files, it would also help me to debug the issue.

Thanks!

PJpb commented 6 years ago

Works both as CLI and GUI with demultiplexing etc. (tested only on one sample though)

drivenbyentropy commented 6 years ago

Perfect. Closing ticket.