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drivenbyentropy / aptasuite

A full-featured bioinformatics software collection for the comprehensive analysis of aptamers in HT-SELEX experiments.
https://drivenbyentropy.github.io/
GNU General Public License v3.0
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AptaMUT #48

Closed PJpb closed 6 years ago

PJpb commented 6 years ago

Hi there! Thanks for the constant updates, nice to see some implementations I've suggested :)

Would you mind explaining the AptaMUT a little bit closer? I can't get to work it out well...

Thanks! PJ

drivenbyentropy commented 6 years ago

Hi,

AptaMut is explained in detail here. In principle, AptaMut attempts at estimating the likelihood that a mutant in a cluster with was measured to be of increased cycle-to-cycle enrichment compared to the parent sequence is truly a better binder as the wildtype. It does so by providing you with a log-score reflecting the log of the ratio between the expected enrichment of that mutant and the measured one.

Hope this helps.

PJpb commented 6 years ago

It does, thank you!