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drivenbyentropy / aptasuite

A full-featured bioinformatics software collection for the comprehensive analysis of aptamers in HT-SELEX experiments.
https://drivenbyentropy.github.io/
GNU General Public License v3.0
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aptasuite take a longgg time #72

Closed samoudj closed 4 years ago

samoudj commented 4 years ago

Hello,

I am working with aptasuite where i have to execute the program on 2000 sequences. But it is taking too much time, and when the computer is in sleep mode the program stops running.

is it normal for the program to run more than 15h? Thank you Samia

drivenbyentropy commented 4 years ago

Hi Samia,

2000 sequences should be no issue for Aptasuite and should be processed withing seconds. Can you provide me with further information on what you are trying to achieve?

Thank you.

rihabbbb commented 4 years ago

hello i have the same issue, for me i try to analyse my illumina Miseq result it is about 5 generationS of SELEX and it take more than 24h and did'nt get the result, after 20 h it is in the thread 82 i don't know where is the problem exactly

samoudj commented 4 years ago

Hello, like I said before I am working on the sequencing data . where i have 5 generations from illumina MiSeq sequencing (I have many sequences with different length in each data ). for each generation i have 2 files fastq R1 and R2. And I have my two primers from 5' to 3': Forward primer TGGCTCTGGCTTCCGTCC

Reverse primer GCTGCTTCGGCTCTCTCG

I entered all these data in the program (the 10 fastq files of each generation and the 2 primers). within few seconds the program was completed. where they tell me to click on Finish to show the results. Once I do that the program continues to run for several days.

I attached to this email a screenshot showing the problem. I would really appreciate if you can help me to solve this problem because it is very important.

Sincerly, Samia

Le jeu. 15 août 2019 à 13:20, drivenbyentropy notifications@github.com a écrit :

Hi Samia,

2000 sequences should be no issue for Aptasuite and should be processed withing seconds. Can you provide me with further information on what you are trying to achieve?

Thank you.

— You are receiving this because you authored the thread. Reply to this email directly, view it on GitHub https://github.com/drivenbyentropy/aptasuite/issues/72?email_source=notifications&email_token=AFNCUAHYP42PVAYQBGELOJLQEWF6FA5CNFSM4IL7HW5KYY3PNVWWK3TUL52HS4DFVREXG43VMVBW63LNMVXHJKTDN5WW2ZLOORPWSZGOD4MN3XA#issuecomment-521723356, or mute the thread https://github.com/notifications/unsubscribe-auth/AFNCUAHLI4M4GB6W2TJBSBTQEWF6FANCNFSM4IL7HW5A .

samoudj commented 4 years ago

please can you answer to my question?? Thank you

Le lun. 19 août 2019 à 09:52, djerroud samia djerroudsamia@gmail.com a écrit :

Hello, like I said before I am working on the sequencing data . where i have 5 generations from illumina MiSeq sequencing (I have many sequences with different length in each data ). for each generation i have 2 files fastq R1 and R2. And I have my two primers from 5' to 3': Forward primer TGGCTCTGGCTTCCGTCC

Reverse primer GCTGCTTCGGCTCTCTCG

I entered all these data in the program (the 10 fastq files of each generation and the 2 primers). within few seconds the program was completed. where they tell me to click on Finish to show the results. Once I do that the program continues to run for several days.

I attached to this email a screenshot showing the problem. I would really appreciate if you can help me to solve this problem because it is very important.

Sincerly, Samia

Le jeu. 15 août 2019 à 13:20, drivenbyentropy notifications@github.com a écrit :

Hi Samia,

2000 sequences should be no issue for Aptasuite and should be processed withing seconds. Can you provide me with further information on what you are trying to achieve?

Thank you.

— You are receiving this because you authored the thread. Reply to this email directly, view it on GitHub https://github.com/drivenbyentropy/aptasuite/issues/72?email_source=notifications&email_token=AFNCUAHYP42PVAYQBGELOJLQEWF6FA5CNFSM4IL7HW5KYY3PNVWWK3TUL52HS4DFVREXG43VMVBW63LNMVXHJKTDN5WW2ZLOORPWSZGOD4MN3XA#issuecomment-521723356, or mute the thread https://github.com/notifications/unsubscribe-auth/AFNCUAHLI4M4GB6W2TJBSBTQEWF6FANCNFSM4IL7HW5A .

drivenbyentropy commented 4 years ago

Based on the information you provided, I am not sure what the issue is. I am also not seeing the screenshot you mentioned.

Could you please provide me with the log file that is automatically generated every time AptaSuite is run. You can find instructions on how to obtain the log file in the Wiki of this project under Report an Issue.

samoudj commented 4 years ago

hello,

here is our log file

System Information: Java Version: 1.8.0_201 JavaFX Version: null OS Name: Linux OS Version: 4.10.0-32-generic OS Architecture: amd64 OS Architecture Model: 64 CPU cores: 8 System Memory: 758120448 Mb

AptaSuite version read from file. Version is 0.9.4

Reading configuration from file.

Instantiating MapDBAptamerPool

Searching for existing datasets in /home/ubuntu/Documents/ribah_projet/19082019adnmod/pooldata

Found a total of 1 files on disk.

Processing /home/ubuntu/Documents/ribah_projet/19082019adnmod/pooldata/data0000.mapdb

Reading inverse view from /home/ubuntu/Documents/ribah_projet/19082019adnmod/pooldata/inverse_data0000.mapdb

Reading bounds data from /home/ubuntu/Documents/ribah_projet/19082019adnmod/pooldata/bounds_data0000.mapdb

Total number of aptamers in file: 0 Total number of aptamers: 0 Processed in 0.495 seconds.

Found and loaded a total of 0 aptamers from disk.

AptamerPool instantiation took 0.529 seconds

Processing selection cycle G0

9:40:12 | CONFIG | Thread-4: lib.aptamer.datastructures.MapDBSelectionCycle Reading from file '/home/ubuntu/Documents/ribah_projet/19082019adnmod/cycledata/0_G0.mapdb' for selection cycle G0.

Processing selection cycle G3

9:40:12 | CONFIG | Thread-4: lib.aptamer.datastructures.MapDBSelectionCycle Reading from file '/home/ubuntu/Documents/ribah_projet/19082019adnmod/cycledata/1_G3.mapdb' for selection cycle G3.

Processing selection cycle G6

9:40:12 | CONFIG | Thread-4: lib.aptamer.datastructures.MapDBSelectionCycle Reading from file '/home/ubuntu/Documents/ribah_projet/19082019adnmod/cycledata/2_G6.mapdb' for selection cycle G6.

Processing selection cycle G9

9:40:12 | CONFIG | Thread-4: lib.aptamer.datastructures.MapDBSelectionCycle Reading from file '/home/ubuntu/Documents/ribah_projet/19082019adnmod/cycledata/3_G9.mapdb' for selection cycle G9.

Processing selection cycle G11

9:40:13 | CONFIG | Thread-4: lib.aptamer.datastructures.MapDBSelectionCycle Reading from file '/home/ubuntu/Documents/ribah_projet/19082019adnmod/cycledata/4_G11.mapdb' for selection cycle G11.

Reading metadata file from '/home/ubuntu/Documents/ribah_projet/19082019adnmod/metadata.mapdb

Loading took 907 milliseconds

[9:40:13 | INFO | JavaFX Application Thread]: gui.core.RootLayoutController$3 Initializing GUI elements

Written Configuration file to /home/ubuntu/Documents/ribah_projet/19082019adnmod/configuration.aptasuite

[9:40:13 | SEVERE | JavaFX Application Thread]: gui.core.RootLayoutController javafx.fxml.LoadException: file:/home/ubuntu/Documents/emre_project/aptasuite-0.9.4/aptasuite-0.9.4.jar!/gui/core/experiment/overview/ExperimentOverviewRandomizedRegionSizeDistribution.fxml file:/home/ubuntu/Documents/emre_project/aptasuite-0.9.4/aptasuite-0.9.4.jar!/gui/core/experiment/overview/ExperimentOverviewRoot.fxml:328

at javafx.fxml.FXMLLoader.constructLoadException(FXMLLoader.java:2601) at javafx.fxml.FXMLLoader.loadImpl(FXMLLoader.java:2571) at javafx.fxml.FXMLLoader.loadImpl(FXMLLoader.java:2441) at javafx.fxml.FXMLLoader.access$2700(FXMLLoader.java:103) at javafx.fxml.FXMLLoader$IncludeElement.constructValue(FXMLLoader.java:1143) at javafx.fxml.FXMLLoader$ValueElement.processStartElement(FXMLLoader.java:746) at javafx.fxml.FXMLLoader.processStartElement(FXMLLoader.java:2707) at javafx.fxml.FXMLLoader.loadImpl(FXMLLoader.java:2527) at javafx.fxml.FXMLLoader.loadImpl(FXMLLoader.java:2441) at javafx.fxml.FXMLLoader.loadImpl(FXMLLoader.java:3214) at javafx.fxml.FXMLLoader.loadImpl(FXMLLoader.java:3175) at javafx.fxml.FXMLLoader.loadImpl(FXMLLoader.java:3148) at javafx.fxml.FXMLLoader.loadImpl(FXMLLoader.java:3124) at javafx.fxml.FXMLLoader.loadImpl(FXMLLoader.java:3104) at javafx.fxml.FXMLLoader.load(FXMLLoader.java:3097) at gui.core.RootLayoutController.addExperimentOverviewTab(RootLayoutController.java:607) at gui.core.RootLayoutController.lambda$showInitialTabs$5(RootLayoutController.java:313) at com.sun.javafx.application.PlatformImpl.lambda$null$397(PlatformImpl.java:295) at java.security.AccessController.doPrivileged(Native Method) at com.sun.javafx.application.PlatformImpl.lambda$runLater$398(PlatformImpl.java:294) at com.sun.glass.ui.InvokeLaterDispatcher$Future.run(InvokeLaterDispatcher.java:95) at com.sun.glass.ui.gtk.GtkApplication._runLoop(Native Method) at com.sun.glass.ui.gtk.GtkApplication.lambda$null$203(GtkApplication.java:139) at java.lang.Thread.run(Thread.java:748) Caused by: java.lang.reflect.InvocationTargetException at sun.reflect.NativeMethodAccessorImpl.invoke0(Native Method) at sun.reflect.NativeMethodAccessorImpl.invoke(NativeMethodAccessorImpl.java:62) at sun.reflect.DelegatingMethodAccessorImpl.invoke(DelegatingMethodAccessorImpl.java:43) at java.lang.reflect.Method.invoke(Method.java:498) at sun.reflect.misc.Trampoline.invoke(MethodUtil.java:71) at sun.reflect.GeneratedMethodAccessor1.invoke(Unknown Source) at sun.reflect.DelegatingMethodAccessorImpl.invoke(DelegatingMethodAccessorImpl.java:43) at java.lang.reflect.Method.invoke(Method.java:498) at sun.reflect.misc.MethodUtil.invoke(MethodUtil.java:275) at javafx.fxml.FXMLLoader.loadImpl(FXMLLoader.java:2566) ... 22 more Caused by: java.util.NoSuchElementException: No value present at java.util.Optional.get(Optional.java:135) at gui.core.experiment.overview.ExperimentOverviewRandomizedRegionSizeDistributionController.computeStatistics(ExperimentOverviewRandomizedRegionSizeDistributionController.java:90) at gui.core.experiment.overview.ExperimentOverviewRandomizedRegionSizeDistributionController.initialize(ExperimentOverviewRandomizedRegionSizeDistributionController.java:55) ... 32 more

9:40:19 | INFO | Thread-8: gui.core.aptamer.pool.AptamerPoolRootController$1 Initializing data structures

Preparing to sort data

Retrieving data for selection cycle G11

Prioritizing data

Sorting data

[9:40:24 | INFO | JavaFX Application Thread]: gui.core.motifs.MotifAnalysisRootController Initializing Motif Analysis Content

[9:41:54 | INFO | Thread-10]: gui.core.aptamer.pool.AptamerPoolRootController$1 Initializing data structures

[9:41:54 | INFO | Thread-10]: gui.core.aptamer.pool.AptamerPoolRootController Preparing to sort data

[9:41:54 | INFO | Thread-10]: gui.core.aptamer.pool.AptamerPoolRootController Retrieving data for selection cycle G11

[9:41:54 | INFO | Thread-10]: gui.core.aptamer.pool.AptamerPoolRootController Prioritizing data

[9:41:54 | INFO | Thread-10]: gui.core.aptamer.pool.AptamerPoolRootController Sorting data

[9:53:51 | INFO | Thread-12]: gui.core.aptamer.family.analysis.AptamerFamilyAnalysisRootController$10 Initializing AptaCluster

[9:53:52 | INFO | JavaFX Application Thread]: gui.core.motifs.MotifAnalysisRootController Initializing Motif Analysis Content

Instantiating MapDBStructurePool

Searching for existing datasets in /home/ubuntu/Documents/ribah_projet/19082019adnmod/structuredata

Found a total of 1 files on disk.

Processing file /home/ubuntu/Documents/ribah_projet/19082019adnmod/structuredata/data0000.mapdb

[9:53:54 | CONFIG | Thread-13]: lib.aptamer.datastructures.MapDBStructurePool Found and loaded file /home/ubuntu/Documents/ribah_projet/19082019adnmod/structuredata/data0000.mapdb Total number of aptamers in file: 0 Total number of aptamers: 0

Found and loaded a total of 0 structures on disk.

[9:53:54 | CONFIG | Thread-13]: lib.aptamer.datastructures.MapDBStructurePool StructurePool instantiation took 0.043 seconds

Starting AptaTRACE

[9:54:00 | INFO | Thread-14]: gui.core.aptamer.pool.AptamerPoolRootController$1 Initializing data structures

[9:54:00 | INFO | Thread-14]: gui.core.aptamer.pool.AptamerPoolRootController Preparing to sort data

[9:54:00 | INFO | Thread-14]: gui.core.aptamer.pool.AptamerPoolRootController Retrieving data for selection cycle G11

[9:54:00 | CONFIG | Thread-12]: lib.aptamer.datastructures.MapDBClusterContainer Creating new file '/home/ubuntu/Documents/ribah_projet/19082019adnmod/clusterdata/clusters.mapdb' for cluster storage.

[9:54:00 | INFO | Thread-12]: gui.core.aptamer.family.analysis.AptamerFamilyAnalysisRootController$10 Initialization Complete

[9:54:00 | INFO | Thread-14]: gui.core.aptamer.pool.AptamerPoolRootController Prioritizing data

[9:54:00 | INFO | Thread-14]: gui.core.aptamer.pool.AptamerPoolRootController Sorting data

[9:55:00 | INFO | Thread-16]: gui.core.aptamer.pool.AptamerPoolRootController$1 Initializing data structures

[9:55:00 | INFO | Thread-16]: gui.core.aptamer.pool.AptamerPoolRootController Preparing to sort data

[9:55:00 | INFO | Thread-16]: gui.core.aptamer.pool.AptamerPoolRootController Retrieving data for selection cycle G11

[9:55:01 | INFO | Thread-16]: gui.core.aptamer.pool.AptamerPoolRootController Prioritizing data

[9:55:01 | INFO | Thread-16]: gui.core.aptamer.pool.AptamerPoolRootController Sorting data

[9:55:11 | INFO | Thread-22]: gui.core.aptamer.pool.AptamerPoolRootController$1 Initializing data structures

[9:55:11 | INFO | Thread-22]: gui.core.aptamer.pool.AptamerPoolRootController Preparing to sort data

[9:55:11 | INFO | Thread-22]: gui.core.aptamer.pool.AptamerPoolRootController Retrieving data for selection cycle G11

[9:55:11 | INFO | Thread-22]: gui.core.aptamer.pool.AptamerPoolRootController Prioritizing data

[9:55:11 | INFO | Thread-22]: gui.core.aptamer.pool.AptamerPoolRootController Sorting data

[9:55:15 | INFO | JavaFX Application Thread]: gui.core.motifs.MotifAnalysisRootController Initializing Motif Analysis Content

[9:55:20 | INFO | Thread-24]: gui.core.aptamer.pool.AptamerPoolRootController$1 Initializing data structures

[9:55:20 | INFO | Thread-24]: gui.core.aptamer.pool.AptamerPoolRootController Preparing to sort data

[9:55:20 | INFO | Thread-24]: gui.core.aptamer.pool.AptamerPoolRootController Retrieving data for selection cycle G11

[9:55:20 | INFO | Thread-24]: gui.core.aptamer.pool.AptamerPoolRootController Prioritizing data

[9:55:20 | INFO | Thread-24]: gui.core.aptamer.pool.AptamerPoolRootController Sorting data

[9:57:54 | INFO | Thread-28]: gui.core.aptamer.pool.AptamerPoolRootController$1 Initializing data structures

[9:57:54 | INFO | Thread-28]: gui.core.aptamer.pool.AptamerPoolRootController Preparing to sort data

[9:57:54 | INFO | Thread-28]: gui.core.aptamer.pool.AptamerPoolRootController Retrieving data for selection cycle G11

[9:57:55 | INFO | Thread-28]: gui.core.aptamer.pool.AptamerPoolRootController Prioritizing data

[9:57:55 | INFO | Thread-28]: gui.core.aptamer.pool.AptamerPoolRootController Sorting data

[9:59:18 | INFO | Thread-30]: gui.core.aptamer.pool.AptamerPoolRootController$1 Initializing data structures

[9:59:18 | INFO | Thread-30]: gui.core.aptamer.pool.AptamerPoolRootController Preparing to sort data

[9:59:18 | INFO | Thread-30]: gui.core.aptamer.pool.AptamerPoolRootController Retrieving data for selection cycle G11

[9:59:19 | INFO | Thread-30]: gui.core.aptamer.pool.AptamerPoolRootController Prioritizing data

[9:59:19 | INFO | Thread-30]: gui.core.aptamer.pool.AptamerPoolRootController Sorting data

[10:01:39 | INFO | JavaFX Application Thread]: gui.core.motifs.MotifAnalysisRootController Initializing Motif Analysis Content

[10:01:41 | INFO | Thread-32]: gui.core.aptamer.pool.AptamerPoolRootController$1 Initializing data structures

[10:01:41 | INFO | Thread-32]: gui.core.aptamer.pool.AptamerPoolRootController Preparing to sort data

[10:01:41 | INFO | Thread-32]: gui.core.aptamer.pool.AptamerPoolRootController Retrieving data for selection cycle G11

[10:01:41 | INFO | Thread-32]: gui.core.aptamer.pool.AptamerPoolRootController Prioritizing data

[10:01:41 | INFO | Thread-32]: gui.core.aptamer.pool.AptamerPoolRootController Sorting data

[10:01:53 | INFO | Thread-38]: gui.core.aptamer.pool.AptamerPoolRootController$1 Initializing data structures

[10:01:53 | INFO | Thread-38]: gui.core.aptamer.pool.AptamerPoolRootController Preparing to sort data

[10:01:53 | INFO | Thread-38]: gui.core.aptamer.pool.AptamerPoolRootController Retrieving data for selection cycle G11

[10:01:53 | INFO | Thread-38]: gui.core.aptamer.pool.AptamerPoolRootController Prioritizing data

[10:01:53 | INFO | Thread-38]: gui.core.aptamer.pool.AptamerPoolRootController Sorting data

[10:05:35 | INFO | Thread-40]: gui.core.aptamer.pool.AptamerPoolRootController$1 Initializing data structures

[10:05:35 | INFO | Thread-40]: gui.core.aptamer.pool.AptamerPoolRootController Preparing to sort data

[10:05:35 | INFO | Thread-40]: gui.core.aptamer.pool.AptamerPoolRootController Retrieving data for selection cycle G11

[10:05:35 | INFO | Thread-40]: gui.core.aptamer.pool.AptamerPoolRootController Prioritizing data

[10:05:35 | INFO | Thread-40]: gui.core.aptamer.pool.AptamerPoolRootController Sorting data

[10:05:37 | INFO | JavaFX Application Thread]: gui.core.motifs.MotifAnalysisRootController Initializing Motif Analysis Content

[10:42:12 | INFO | Thread-42]: gui.core.aptamer.pool.AptamerPoolRootController$1 Initializing data structures

[10:42:12 | INFO | Thread-42]: gui.core.aptamer.pool.AptamerPoolRootController Preparing to sort data

[10:42:12 | INFO | Thread-42]: gui.core.aptamer.pool.AptamerPoolRootController Retrieving data for selection cycle G11

[10:42:12 | INFO | Thread-42]: gui.core.aptamer.pool.AptamerPoolRootController Prioritizing data

[10:42:12 | INFO | Thread-42]: gui.core.aptamer.pool.AptamerPoolRootController Sorting data

[10:43:01 | INFO | Thread-46]: gui.core.aptamer.pool.AptamerPoolRootController$1 Initializing data structures

[10:43:01 | INFO | Thread-46]: gui.core.aptamer.pool.AptamerPoolRootController Preparing to sort data

[10:43:01 | INFO | Thread-46]: gui.core.aptamer.pool.AptamerPoolRootController Retrieving data for selection cycle G11

[10:43:01 | INFO | Thread-46]: gui.core.aptamer.pool.AptamerPoolRootController Prioritizing data

[10:43:01 | INFO | Thread-46]: gui.core.aptamer.pool.AptamerPoolRootController Sorting data

[10:43:12 | INFO | JavaFX Application Thread]: gui.core.motifs.MotifAnalysisRootController Initializing Motif Analysis Content

[10:49:06 | INFO | Thread-50]: gui.core.aptamer.pool.AptamerPoolRootController$1 Initializing data structures

[10:49:06 | INFO | Thread-50]: gui.core.aptamer.pool.AptamerPoolRootController Preparing to sort data

[10:49:06 | INFO | Thread-50]: gui.core.aptamer.pool.AptamerPoolRootController Retrieving data for selection cycle G11

[10:49:07 | INFO | Thread-50]: gui.core.aptamer.pool.AptamerPoolRootController Prioritizing data

[10:49:07 | INFO | Thread-50]: gui.core.aptamer.pool.AptamerPoolRootController Sorting data

[10:49:10 | INFO | JavaFX Application Thread]: gui.core.motifs.MotifAnalysisRootController Initializing Motif Analysis Content

Le mar. 20 août 2019 à 12:00, drivenbyentropy notifications@github.com a écrit :

Based on the information you provided, I am not sure what the issue is. I am also not seeing the screenshot you mentioned.

Could you please provide me with the log file that is automatically generated every time AptaSuite is run. You can find instructions on how to obtain the log file in the Wiki of this project under Report an Issue https://github.com/drivenbyentropy/aptasuite/wiki/Report-an-Issue.

— You are receiving this because you authored the thread. Reply to this email directly, view it on GitHub https://github.com/drivenbyentropy/aptasuite/issues/72?email_source=notifications&email_token=AFNCUAH2FH72BB2BM76DAYLQFQILRA5CNFSM4IL7HW5KYY3PNVWWK3TUL52HS4DFVREXG43VMVBW63LNMVXHJKTDN5WW2ZLOORPWSZGOD4WZLXA#issuecomment-523081180, or mute the thread https://github.com/notifications/unsubscribe-auth/AFNCUACMLJMSQIDI57M7HKLQFQILRANCNFSM4IL7HW5A .

drivenbyentropy commented 4 years ago

Based on this log it looks like something went wrong during the importing of the sequencing data into AptaSuite. The log states 

[9:40:12 | CONFIG | Thread-4]: lib.aptamer.datastructures.MapDBAptamerPool
Total number of aptamers in file: 0
Total number of aptamers: 0
Processed in 0.495 seconds.

which typically indicates that the parsing was not successful.

The first thing I would check is if the primer orientation is according to the requirements of the parser. AptaSuite expects the primers to be provided in the orientation and order in which they appear in the sequencing data which sometimes corresponds to the reverse complement.

samoudj commented 4 years ago

these are my primers: Forward primer TGGCTCTGGCTTCCGTCC

Reverse primer GCTGCTTCGGCTCTCTCG

this is an example of one my fastq files (R1):

@M03555:476:000000000-CJGL9:1:1102:16972:2053 1:N:0:CTGTACGTGA TGGCCTGGCTTCCGTCCACCAGCCCCGACGAGTGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATTTTTGCATCGATTCCTCAAATGTGCAACCAAACTCGCACTAGTTGGTCCCGTGTTAGTACTGCAGACTGACACGTACATGTGATGTTGTGGTTATATAGATTATTATAGTTTCTTACACTGTGCTTGTTGTGTACTA + AA@AAF111>11BAB1EA100BAA0AEEE?/E//DF1GE/A/A1ACF0BFHHHFFGFGHHG1FEFEBFEGHG@E /00?/2BF1GF/B0>1/>/0?>F1BGGF01BB0/////011?1<1111/0/11111111111111110...<00<----00000/.00.-:...00000;000/9./0//00../..;0;0;000009.-999/////////9//////:;////9/9//9/:///-;----//// @M03555:476:000000000-CJGL9:1:1102:21633:2759 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCAGCAGGGGGAAAGGACAACGGACACCCAGCGCCCCCGCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATGCATTTATTATTCAATACTACATAACGTGACATTGTCGGCTTAGTTTGCACATAGTCTCTTGATTTGAACTACCCTCTATAGTTGCTACCACGACACCAGACGAATG + AA>ABFFFFB4CBGEGGEEGGGHHCFCAEHHFFGGCHGGAEEHGGGEHGGGCEGEFCGGG/EAEHHEE//EF3GHCGHHGHHGFFFHFGFFHFFFHGHHH21FGFEEF1FGHG/F0FGCEGHGDGHHHHGAF=<GCCDC---.00000;0;009000000/;B09////...../////9.--9-//9////////////:///////9///////../.9///////////.9/.----.....--;9/ @M03555:476:000000000-CJGL9:1:1102:19032:3662 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCCCCGCGACAGGAAGGACACGCAAACAAGCAACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGACTCTCGTATGCCGTCTTCTGCTTGAAAACAATACCACCCCCCTCACCATCTCGTTGTTTTCACCTCACTCTTACTTTTTTCTCGTTCATTCTCCATGCTCATCCCCACGTGCTTACATGCACCCCCTCGCTCGCCTACTCTCTTTCTTGTC +

A?3>BB4CFFFFFFGFEE4EAEE?EAEG2FAA1F2FEGGCEHAEBH2FGGAEE/BAGHE/E>EHFB1??GHH/BG3F4FEHGFHEGG/EGHFFFHBBC/21BFEG<G<G1@E /?F1<1?GB1011?0.F11111<..----....00/00.;./:.//000009/.;000000=;0:-900..../0000///;9/////9/.9..-....////9/////9--;.--.9---.9.////9//////// @M03555:476:000000000-CJGL9:1:1102:14274:3724 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACACGTTTCAAGGCCATCAGATGTGACAAATCAAGGTGCAGTTATGAACTGGAGCCGTATCGATAGCGATGCACTGGACGGATGTTAATACTACGCAGAGTTGATGAGTGTTAGTTGGTTGACAAGGTGTGTGTAATACGATGTTATAG + A??11BD111>BB1FE1EEA0A0EAHEECCGGHCHCBFGFCHBFFHHFH1EFGGFG//BFGHEGFGFG1DBCEECFFDEEEB?FHDBGGEC1BD0EECG////0/000122?1///0111111111111<1<1111<11111111111=00//.<<-.........0--;..0009//.--..-90000000009-.---9//9////////9/9/9/---9///--//;----/////--;-;///// @M03555:476:000000000-CJGL9:1:1102:21769:3779 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCACAGACCCCCCAGGCGGACACAGCAGCCCCAGGACCGCAGCCGACCTACCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATGGTCTCGAGTGTTGAAAGTAAAAGTGACATTACGTGTGAACAGTGCCTGTCTGCATGTGGGATTCCAGACATTCATTGTAAATTGTCATCTACAT + AAA1>1CFF111BFFGEECCAGCFCCCEECEGCECEGCHCHHFHFHA//EFEHHECEEEHGG/EGHHGHC>EEE/EGFC@CCGHHHAF0GEHGHGGHFHFHHGFHGFECHHG <GG0FFGHGHFHGGBHFFG<<GFGGHHHHH;00CAC@ -;---.009;9/9/-:-//-//;/;//////////////-9-/-;/////////////////////9-//;////-9///9///////////9//////;/ @M03555:476:000000000-CJGL9:1:1102:11460:3943 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCGCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACGCTGCTTTTTCATCAATACATTGATTTGCTTATACTTTAGATCACATACGTACTCCAACGTCTGTGTTATATCTCTGTTTCGTGTGTACACTCCATCGTGCCGTATTATGTATCGTGTCAATCAGCTTGCGTTGCATTCTCGTC + BBBBBF>DFFBFGFGGGGGGGCGEGCDDFGGFGCCGFFHGHGFHGFGHHHEGHHHHGEEEEHHFHHFGBFGBDGGBEEBED/EEFFFDGHHHG?BEECEGG/////////<111?10111111>111111<110<=0=000000=00000/0000;00..0/:;/.:./.00;/0000000000;00.....;00000/B/00....9-9..///9////.;.;.//////////.-..-.//////... @M03555:476:000000000-CJGL9:1:1102:20613:4021 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCCAGCACGGGGCACCCGGCGCGCATAGGAGACCAGAGGGACCCCCCCGACGACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATGAATGCACATAGTCAGTCAGGAGAGACATGTATTAGGAATGAACACGGAATTAGCGTAGAGTGGAACAGACAGACGACGACACTGTTGAATTTAG + BBBBBFFB4@FFEEEGCA2FCFBFE ?A2E2EEEEEGGE0ECGGFGB31?FFBGEF11FE>EEEGCEECCC//B<C/CFH@ <C?CGGHHGEHHFGHFGFHG0<GDG/DEHGHFGFGHGFECCCHFHGGGFHGGG-FEBFGGGGFFGGFAB?9--;.//////////////;/:///./.:....///////////////;/..--..////-..-./9;.///.//9../.--------/////9////// @M03555:476:000000000-CJGL9:1:1102:21555:4283 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCCGCTGCCTACCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATATCGTATGCCGTCTTCTGCTTGAAAAAAACTAGGAACTGCATCCAAAACTAACCCACTATATTACTCGAAATGGAGGCAGAATTCATAGTGTAGCGGTGTTATTTTGCGTTATTATGAAAAATACCATAGTCATAGGCGGCCTCCTTGCTTTGTACTGACACGTCGTAGC + ABAAABF44CF?FGGGFGE?EEGDFHDC2EEC?EAFDA2E00AGGHA1FGCBFGBAGGHDD5FGFE11BAGHG@GHHEAG4 @BFHEB34@EFEFE?G4FF3F033?BE//1B10B1?1D11@G0FF1/0<1F10/?/?01111@ ?11..0.>1110.<<../<0=0000000=DB-:.-..0;00000-....90000;00.;;;FB0009;000;;9--;>..;:///9//9//;;/////.9.-;... @M03555:476:000000000-CJGL9:1:1102:14066:4687 1:N:0:CTGTACGTGA GTTGAACACGACCAGAAAACTGGCCTAACGACGTTTGGTCAGTTCCATCAACATCATAGCCAGATGCCCAGAGATTAGAGCGCATGACAAGTAAAGGACGGTTGTCAGCGTCATAAGAGGTTTTACCTCCAAATGAAGAAATAACATCATGGTAACGCTGCATGAAGTAATCACGTTCTTGGTCAGTATGCAAATTAGCATAAGCAGCTTGCAGACCCATAATGTCAATAGATGTGGTAGAAGTCGTCAT + 1AAAAFF1CA@1EEGAA1CGB1BCGGGHFECEEEFHAFEFBGHHHHGHHHFHHFHFGHFFHHFHGFEHFCFEEGGHHFGFHGEEFCFHHHGHHHFGFFGGCGF /?FGFGEEGGFFDGGHHHGFGHHGHHHHGHHHHHHBCGFFHHHHHHHHHHHHHGGGGGGGHFHHHEFGFFHGHHHGHHHHHGHHHHHHGHHHHBHGHHGHFFHHHGGCGFGGGFGGGEFFFFGGBFBFFGF;0BBFGEFFFFFFFFF @M03555:476:000000000-CJGL9:1:1102:22433:5097 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCGAGAAGCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGACTCTCGTATGCCGTCTTCTGCTTGATAAAAACCATATTTTACTACCTTTTCACTTTTTTTAGATTATTCATAGTGTCTGCTCGAGTCTGTTGATTGTCATTTTTCCTGTTCGTTTATTTCGTCTACCTGCTTTTTTCTCTGTAATGCTTCTCCTTATATACTTACTATTCTTCGT + AB@A3CFBCBFFGGGFFAA2EE2GFAE ?EEEAEAFE20A?FABGEEAFG1GHHGFGHHFHHF@FHEF4FEF1BF11B3BFEFAGBFFCEEFGGGB4FEGH4F4E3B ///334422B1121B1BF12222>11///01111?@1?111??11==1=.->1<<1<<00=000000<00=/000000../..0=00/....0000./0000-/;000000;0000900;0000000/;////9;//;//.. @M03555:476:000000000-CJGL9:1:1102:15282:5266 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCGAGCAAGGCCCCGTAAAACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGATGTTAGAAATAGTTATTGTAAATAGTACATGTTTGGTCACTGAACGTAGGATATGTACGTGATTCTTCGGTGTAGCAGCGTGTTGCGATCACAGTTACCATGGTCTGATGCTGTCGTCTCTTTTTGG + AAA3BCDFBF4AGEGGGGGGGGHHGHHGGGGCEDCHGGGGGFGGGCEA0EHGHFHHHFHHHGHHHHGFHHHHGFHGHHGFHHGHGEHDGHGFEHFHGG?EFGBGGGGHHEHHHH/@@<<-.<111111<1111<1<<000000<000000<00000/:.00/0000/.....00000000.//./00000...9.0;000/-...../--...////////////;//////9////9....////;-- @M03555:476:000000000-CJGL9:1:1102:16562:6082 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACTTTTTACGTTGGGTTGAACAAGTAATCGGGATTCGGAGAAAGTGTCGCAGATCATGGAACTAGTAATGTAGAACTTCCCTTGTATAGTTCTTTCGTTGATTGTATTTGCTATAGTTACTAGTGTATGTTGTCTCTCAGACAATGT + AAA>AFFBC4CFBEEGFEEEEEGEEFEDGGEAE2ACHHC3FCHDEGED5GBGFHFHCFEEEGFGHGFFEE55DDFGCGFHE/?>GFGHFBGFH?@?3?@E/<<//112>1/1/00<0////011?0011111..-<.<0...--.//0000..-.:0/000000/000000000000000000/00/900000000000..:/.//////////:/9//////9//9:9//////;///9/////..//; @M03555:476:000000000-CJGL9:1:1102:22522:6191 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCAAGACAGGTGCGAACCCTGAGAGAGCCGAAGCAGCAGACCAAGCCTCTGCTACCGTACTGTACGTGAATCTCGTATCCCGTCTTTCGCCTGTATATCCAAACCCTCCCCTCTCATCCTCTCTCTCTGTCTCTCTTTTCTGCTCTTCTCTCTTTTCTCTGTTCTCCTCTGCTCTTTTCTCGCACCTCCCTCTCTCACGCTGCTTCCTCTTTCTCTTTTCTTTCCGTTCTTCGC + A1>A@11BC1B1BA1F1EABA0FABFGHCC0A0A0A0B1CECGE ////E1AA0FCAGB/G//@0F11F110>F/>FD2GFFGCA/G1F1FE/00>>/?EG/22////?<>2>22B211/?<0//?/0/@0@ @F<<1<?11111111?<1<1111111111111FF<1>1>F00<000<000=/0000<=00;000..---/.;.//;99000..;..000/;09090;0000000009/:/9--;/-- @M03555:476:000000000-CJGL9:1:1102:7769:6296 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCGCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATAAGCTTGAAACTGTCTATAGAGCCACAGATGTGATAGTATCACATTCTTTTCCTCACCGCCATTACTCGTTCTAAGCTACTTGTTTATCATTTTCTGTGTATCTAATGAGCTAGCTTGAAACATTTGCCATTAACACAATACAGTT + BBABBFFFFFBF4FEBAEC?EEFE?FEGFFCEGGGHHGHFAGFHGHEHHHBFFHHH0FCECFDDHH2E1FB44@ ?BECCFDF?EHGBGGGHHE0B?30<E//>0222222202222111111@ <111000?0111<111111111<11111100000000...---.:0000:..;.000/000000;0/0900000000000000090009000090/00/.//////;///////////..///;//: @M03555:476:000000000-CJGL9:1:1102:16938:6471 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCGGAGGCAGGTGAGGAGAGCCGACCCGGGGGCAACCGAAGCCGCAAGGGGACGAGAGGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGAATAGTGGCTTTCCCAGTGTGTAGTGTCTGTTCTTCGTTCTTGATCTACTCCCCAGTGTGACCTTGTCATCTTTGTCTATTTCATTCCCCTTGGTCGG + BBBBBFFBFFFFFGCGFFFEEAEEGFGAGBFHGGHGGCC00AEEGGGGG?FF?E/>EFGEE?E/E?EACGCC/B??FBCC??1FCCGHHC0CFFFGG=DHFFFHGCGGHFHHHHHHHAGFFHHFDGBGHC9@FFGFB0FFG.09C.A- ;--.../////..../;BF//:9//.:///99/;;///9/../.;///////////....//:////////////9//;//////;999////.:;.///-- @M03555:476:000000000-CJGL9:1:1102:16892:6779 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCCCGCCACGAGAAGGCCAGAGACAAGGGACGCGCGACGCCAGCCCACGCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAACACACCTCCCTGCTCCACTCCCTCCCCTCTTCCCCCCTTCTCCACCCGTACTCTCCTCCCCCTTTGTCCTTCCTCTTCGCCTCTCCCTCCTTCCCCACTTTCCT + AAAAAFC4C4FFBFEGGCAFE2E?AE?EGGFCEEEGGC2BCFHHGEFGEEGEGGEEE1EF1?G/EEEEEE/<FDFHGG?@@BCGHGCHABGH1?1FDFHD1FC@F AEFHHHGCC.<=0<=CE<G/GD@;:GECF0:G:0.:;CF@ .9..../.../0./00;:0//.........///.9.99..///;.;.-.-.://///9/..9-.//////////////.....///99...//;.9.;.////9 @M03555:476:000000000-CJGL9:1:1102:19994:7040 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCCAGCACTAGCCGCGGGCAACGGGACCCAGGCAAGCCGGCGAACGCCCACGCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAATAAGAATTTTTTCTCTTATTAAGAGTTACTTTATAAGTCAGTTATCTTACATTGCATTCGCCATGCGTCAGTCTGTTGATACTAATCACGCTGCCGCGATGTAC + AAABAFFBCBFFGGGGFGG4BF4AFFGHG?E?EGE?F1E01B>EG1FCGEBEE1C/>E/EEGGGEGGGGGCGCCEGFH/</ACF0FGAGGHHHFFFG1DGFFHDCCCGDGBGBG.:CC.0CGGF.CFCG0;;-9C00B0CF0.0;00;00;0000;;-/C0/;B:B///9/9///9////99/9////9;/;///9;///////9/.;..9/.;;..;///9///9/////////...;9.-;--;:./9 @M03555:476:000000000-CJGL9:1:1102:12051:7410 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCACGGAGACAGGGATGCCACGAACAAGGGAGACATAGAGGCAGCCGCGCGACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATTCCGTCTTCTGCTTGTAAAAAATATATAAGTGTTACTTCTGCTTTCTCTTATAAATGTGTAGTTACCAGCCGTCACATCATAGGTCATCTTACCTTTCTTTGTCGTCGGTGTAGCTGTGTTAA + AA1AAFFBBFFFFGFGGG1BEE0E?E0GGCAGFHHCHGEEE/ABA?/E/FHGGF1FHGGGGGGG/EEEE??C?/C</FE/EEGG0B00B<GGHFFDHHHHBHHFHCCHGGBD1??FF/FG1FGHGGH1>1F>GGG0GB0DDC<0=0=0-.00;0000;0;00:00:0000;00;0009;00090090000900;;9..-9-.-//://////////////9/;////;///-----;:-/9/;///9/// @M03555:476:000000000-CJGL9:1:1102:18295:7437 1:N:0:CTGTACGTGA TGGCTCTGGCTTCGTCCCGGCCGAAGACGGGAGATGAAGAGAATCACAGCGCAGTCCCAGCCAGACACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACACATCTTACTTAGAATGCTAGACATAAGAGTCGACATCAAATGTGTGTATTAATGTATTGGCAGATACGATAGGATGATGTAAGATGACAAAGGAA + BBBBBFFFBFFFGGGGGEEFFFGGGGFHGGGGGGHHHHHHGFHHHHGGHHGGGGGHHHHHHGHHHGHHGEEDFEGHGGGFGGGHHHHGHHHHHHHHHHHHHGHHGGHGHGHFHHFFEGHEHGHGGHEGHGGGHGGHGHHHHGCGHHGGG-;-;....0000000000;00000900000000////-:-..//////;//..//9////////////..///...9.///////:/9////;//9./../ @M03555:476:000000000-CJGL9:1:1102:24541:7443 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCAAAGACGCGGGCAGCAACCGAGAGAGCCGAAGCAGCCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATAACCTTATATGGTTTCTGACTTAATGTCTTACCCCGACTAAGGTTTTTAGCGTTAGTCCATTATGTGTTAGCACCAAGGTCTTGGTTATGTCCTGTGTTTATGTTGTTAGAGTTTCTTTGTTTTTAT + BBBBBFFFFFFFFFCGGGGBFG4CA2EGGGCEDFGHH?AEEE?EHGG?EECFGHHACFFHHHFHHHHHGHHFHHGFAEGFFGHHGHHGFHHHFFCGFFGG?CGEHFF2GHHFGGBCCC<---.000/=00000000:.000;000:000000000//.;----0//000.../0;9...//////////////////.....;//9///9///9/9//////.;9////..////;///:////....9/ @M03555:476:000000000-CJGL9:1:1102:19591:7469 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCGGAGGCGGGAAAACCAAGCCCCACCAGGCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAATAAAAATCTTCCTCTGCGCGAGATCTTAAAATGTTAGATGGTAAGTTTTCCATCCGCATGTTCCGCGCATCTACCAGTTTTGAATCTGTAGTAGTCGTCCTAGGCGCTACACACTCACCTTTA + AA?AAFBDF4CF4FEFEEGE?EEEC?00E11G1F1BFEGGDF?HEFHGE?E/?EHH?/EEGH30?FFGF0?CF3FGFDHHHDG??/DCFGF2??G/?D11GHFFFHFBCCDFF<F1FGHH0G1FA--.00000:/000000000----9-.0000000000;00000/000000000;///:..-..9///.;---9-///////:9/..99/;///;///;/.....////9----/././///.//// @M03555:476:000000000-CJGL9:1:1102:27012:8059 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCGGGCCGCACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAACAAATCTCCCGTTTTAGTGCCCGATGGTCTAACTTTACTTTTCTGTTTCGTCTTCCAGCTGCTCTGCTATTATGTCATGTTCTCACGTCCCCCTTCACTTTCTTATTCCTTGTCCGTCTCTTTTTTTCCCAACTCATCCTTGAT + AAABBF4DFFB?FBEAGEC??E2EAEEG?A101BE1AEA0EFGG1GCFAFA1FBGDFFDB4B2E?G1?BEEG1?GE1FGGFFC/FCFDEGGGEB44BFHHA22?CC///?222222//02/101<110//-..>111111111111000000000.....000:/0//000000000000000000000000.9.....-./0000000000000000///...../////--///;/./////////// @M03555:476:000000000-CJGL9:1:1102:8653:8172 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCCGGCGCCCAGGGAAATCGACGCCACGCCAGGCAGCAAGGCACCAGCAGGCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAATAAATCGTTGTATACATCTCGATGTTGAGGCTCCCATTGTCACGATCGGCCCCTTCAGCCGTTATCTTCTGTATGGCAACTACAAGCTCGTAGTGGAAATG + AAAAAFFBBFFFCF?GGGG0A0A0EAEGGGFFCGHCAECEE/BEEGCCFEHGGEHFGGGHGGHHHAECA?/E@EE <GGG?EGGFHFHEGHHEEH1FHGHHHHFHHGGGGHHHG1/<FAFG1=FFCAC01FCGG..DHEHHHH<CDGGC-.00;00:9./;000000;00.0:0.000//.9..9/000////-------;-9;--////-9--9//99//////--//9//////:99-;---/-/;/// @M03555:476:000000000-CJGL9:1:1102:8229:8802 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACGACGTTTGAAGTGCGGATTCTGTGTATGCGATAGGCAGTGGTTATGTGTATGAGTAGAGTTTAGGGCGTGAGAGTGATTTAACTTCTAGAGAGAACCGATGTATGTAGTGTCGCTATTTTGGAGGTGTGTACGATGTGAGAGTGT + AAABBFF4CBFFBBEFGFGGCCEEC2EHFGCFGGGHFHHHHHGHHHHHFGHHHFHHGGGGHGHBHHCGFEHEFHGGEGFFBEE@ ?FEHHEHF3BB33B/EE/<///////<0<111@ ?1/-<-.11111>1000.....//0.;//0.00;00<00000;0000;00000./---..0/;0000000000000000/..//.9--../;////;///.....;///-//.../.9//....9/////9/: @M03555:476:000000000-CJGL9:1:1102:6564:9238 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCCAACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAATAACCTCTTATAGGTCTTAATATTGTAAAATGTTACTCCGTAATATTCTTCTGTGTTTCCAACACTGTAGTTCATACATCATACTGTATGCTTAATTGTACTGTCGTATTTGTTGTAGCAGTAACTATTCGTACTTTCTACTTATGT + ABAAAFFFFBCFGGCGGGAG42E2EECFGHHE22AECGFGFBGEFAAADFFFFHHGFHCGHGGHFFHEGEG155DFGGGHHGHGEEFFHHGHGHEBB3FH///03333301112221B1112?B22222222221@22 2@1 //0//11111111111<>011111..1111=1<=0<000==000000<0000:00000000000;:...//00;/./0;0000;9000;00///9.009990000000 @M03555:476:000000000-CJGL9:1:1102:7383:9326 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCGGACGACCGCTCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGAACTTGCGCAGGTGAAATGATCAATAAGCAGGACATATCTTGATGCGCAGACTAAACAGTCATGTCGCATCGTGAGGGTATTGCGTGGAGATGCAATCGCTAGAGTGGGGTTACGTGCGGAGGTGAGACTAATA + BAABBFFFFFBFFGGFFCC2?EEEAEAEFGGGGCGEAHHGF?>EGHFGFGGGH1FBEGBHBGFHF?>FEACGFHHHFBA/FDEGFFGBFFHGGCGEFFFGHHF0FG?FC/@<>--.../0000-----./00000;00000;0:00/.../0000000;090-.---./000/0./////;/.9-.-.../...9/////9..-..////////;.9..//9/..-9-./.;..-;---../.9////// @M03555:476:000000000-CJGL9:1:1102:20651:9542 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCAGCACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACCCTCTTTCCTTTCCTTTACTCCTTTTTTCCTTTCTTCCTATTTTTTTTTCTTTTCTTACTCTTTGCTATTTTCCCGTTCTCTCTCTTCTCCCTCTTCCCTCTTACTCTTTCCTATTCCTCCTTCTCTCCTTCCTCTTCTTTTC + AAAAAFB1C11B1BEGCECCFG00AE0AFEHFE/EEAC0EGFB0AB/01DF1FFHHBGFAFHGHBEGHCF0B>DB2B0F/B0@E ///BF2FBH1B10BGD0>///////0?011211@2 @1122111?111111/0>=111111111<1111---;::0000:000:0<00000000;00B0.:./90009000;000/9//009/99-9////////;/////99//9//9/9//;//:/://///;// @M03555:476:000000000-CJGL9:1:1102:8705:9687 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCACCAACGCAAGCAACAACGAAGGACGGGATCGACCCATAAACCGCCGGCCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAACAAACATTATCTCTCCTTGATACTTCTACCCATTACCCAATTATCGACTCTCGTCTCCACCTCCTGTCTCTTCTCTTACGTTTATCTCACCTCTCTCTCACTTCCC + AAAABF4B444CCGEFGFFFGFGD??EEEGFCCEG2EAF?EGG0ECFGGCDC0E1FHFGHEEEEGFCEFGGGC/?A?///>@G2CFCGGHBC02?GFGG1<GFGGF/?G1FF1C0<.>1FGHF?G/DDCAAGEHHHGB0:.:0</...000000000/;000000;000000.0/0000/0.900;0..-.;/:...../9......////////999//;..//./////;././/9/////////. @M03555:476:000000000-CJGL9:1:1102:12244:9872 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCCGCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATTTTGAATGTGTTTACAGAAGACAACATGTAGACTCCGGATGCGTTCCCATAATAGGGAGATCAGCGTCTTGATCTCAAGTGGCGACTAATTCATGGGTAAGCACCTTGCCTGGCAGGTGGCTGATAGTTGCTGACGCAGGAGTG + AA3AACCFFFBFCGGGGGFEEEEF?EFGHHG2AEGHGHHHHHGHGGF5FGHHFH5GFEFFGFDDGHGHHGFB4BGFFACGFDE?EHGFHHEHHHAGBBGFGG/</0<221?1111110011111111000.<01<111110/.<..-<-.:./00;/000:0.:../0000.....000000000000.9----/99/9///.//////9.///////9...-99.....999///////9..---.... @M03555:476:000000000-CJGL9:1:1102:13770:10094 1:N:0:CTGTACGTGA TAAAGCTGGTAAAGGACTTCTTGAAGGTACGTTGCAGGCTGGCACTTCTGCCGTTTCTGATAAGTTGCTTGATTTGGTTGGACTTGGTGGCAAGTCTGCCGCTGATAAAGGAAAGGATACTCGTGATTATCTTGCTGCTGCATTTCCTGAGCTTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTCCTCTGCTGGTATGGTTGACGCCGGATTTGAGAATCAAAAAGAGCTTACTAAAATGCAACTGGAC + BBBBBFDFFFFBGGGGGGGGGGGFGHHHHHGHHEFFFFGHHGGHHHHHHHGHGFGHHHFHHGHHFFHFGHHHHHHHHHHGGFFHEFHHGGHHGGHHHHHHGFGCEHHHHHHFGHHHEHFFHHFGHHHHHHHHHHHHHHHHGHHHHHHHGHHFHHHHHHHHHHGHGGGGGGGGGHHHHHHHHHHHHFHHHHHHHHHGHHHHHHGGGGGGGGFEDGGCEEFGFGGGGGGEFGGGGGGGFFFGGGEFFFFFF? @M03555:476:000000000-CJGL9:1:1102:24319:10186 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCGCAGCACCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACTTCCAATTTATACCCCATCTCCGCCGATCTCCATCTCTTTTTTGTTCTCCATGCTTTATGCTTTTTACCCTATCACACTCTGGACCTACCACTGCCTATTACCTTCTCTTCAGTTTCCGCTGTGTTCGTGATCTTCTC + BBAAAFFFCCFFFGEGGGEEEEFHHHFGGGEE2AG2EEEAEE3BGFFAAEAGFFHBD@FFFHE @EGDGDD@FFGF1F4 @FGEEEGFHFFGGHEFHHHHHHA??3BE/>B///111111111?11?11..00111...----.000000000000--/:0000000/900000000000.00/0/90000///9000...//.///9//9//9//////////////////...-.////.../9////// @M03555:476:000000000-CJGL9:1:1102:4919:10268 1:N:0:TTGTACGTGA AGACACAGGCTTCCATCCGTATAATAGCTTGCCCAGACCAAGTCTCTGCTACCGTATTGTACGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACTTTCTTCCCGTTTTTTTCTCATTTGCCTCTCGTCCTCACACTTCTGCTATTCTATATTTTTCGTTTTGTTTTCTCGTAGCCCTTCGCCTCTTTTCGCCGTCCTCCTCCGTTGTTCTTCCTCTTTTTCGCCCCTTCCCTCCTCACCATTGC + ABABAFCC>AFFGGGGGGGGGGHHHHHHHHGGHGHHDGHGFHHHHHHGHHHHHGGHHHHDFHHHHHHHHFGGGHGGGGGGCFFHFHGHH1FEFHGE//>/344B444?2//03/?//2@ 22222222222/?///11/110<<11111111111=1=<110-</.../</<000.....0/;/:...../0000;9.---..0//9/..0...//:///////99/-...-..././...;/..///// @M03555:476:000000000-CJGL9:1:1102:23313:10618 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCCCCCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAATAAATCTACATTTTCCCCTCTCTCTCCTACTCCAGAACTCCTCCTCTTCCTCTCTTTTCCCTGCCTATTACTTTTTTTCCTCTCTTTTCTGTCTTCCTTCCTCCCATTTTTTTCTTTCGCTTCCTTTCTCTGTGTTCCTTGTTTGCTCT + AA3>AFFFFFFFBFAFFGEF?EAEGG2221BBE0E0AB3FFC3BGGAHGGHHHGHFHHHEFEEEBGGFGFEC1@4F4EFE3 ?4B/?/FFFH44??303B??0233333333333332222/222222222211?1?1001111?00>>11111101111>11<0..<0=00000000--/00<0/0000000000009000/90/.900000-9/;///...../9///9/9///;////////.///// @M03555:476:000000000-CJGL9:1:1102:26419:10696 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCGAGCGCCACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGGTTAATGAAGTGTGTAAAGGCGTAACTAGAGAGAGTCAAGCTAGAACAAGAGAATCAACGATACATCACGTAGATTGGCAGTAGGCGTGGGGGCAATAATGTAGCGTGGATAGAAGAATACAGATTGTAAGTTGGT + BBBBAFFFFF4FBGAFGEECFGGGG2BEEFGFCFDGEEAAECAEGFH1FFCFFGHHHFFHGFHGFEEGHFGHGGFEAG4FHHGFGHGHCFGGE4GBDGFGFB33FC@ /?<</-..111111111<100.00<00.-.-..0<0000.../00:00/00000////.//0;009....-00000..9..///.//./////9.-------./////////-..-./9///9////9/////////9////. @M03555:476:000000000-CJGL9:1:1102:18990:11861 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATATCGTATGCCGTCTTCTGCTTGTAAAAAAAAAAAAGGTATGCTGCTGAATGACACTGCTACAAGCTTGCATCGAATGTCTCATGGCTGCAGCGTGTACTATGCGTTAACTCGCAGAGCCTATATGAAGTGTTTTTTCTCATTTTCTTATATGCTGATTGCTGTCTGTGTGTAATGTTAAC + AB??AFBDF4F?4AEFGEF?2EAEAFDFFC?E?C32GFFF1CEGGFFHGFFFFFAEAAAGFDGDG2211DB@FFHA ?FFD?AEHFBDGGHHH144443E?>//////22222222<1<111?1<1?111<1111.>10.11<.001/00000000./<.<//-..:/000000...9.000.--.-././:00000000000//.-/9/;////////////////////;/;///;//..///////// @M03555:476:000000000-CJGL9:1:1102:26167:12157 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCAGGGGCTCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGATTATAGTAATTAGAAAATGTAAATAGCATAATCAGAGTAAAACTTTACGATGGTAGACATAACTGGTGAGGAGATCAGAGAATAATGGATAAGATGAAGTATAAGAGACATGGAAGCAAACGAGGTGGAGGTGAGGA + AAAAAFCDFFFFEGGGGGGGGGGGGDFEEGFGGGHHCCECEFGGGHFCHFGFBFFEG5GHGHFEFFDHDBDFFFFGGHBGHHGGGFHFCEGHGHHGGHHHGFFB0ECC>////111111111111111101111111111111<11111000000000000.....000000000/00/0/0.:0../00000//0000000000000;0;;000;000://./9./9/.///.:..-9......./.;/ @M03555:476:000000000-CJGL9:1:1102:26032:12278 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGTCTGGATCGGGTATATGAGCCGGGGAGGCCTGCACGACATATGTCGTGGGTTGATCGTAAGCTATAGTGGAAGTGCGTGGAGCGTTGATACCCCCCGTTGTGGTTGTTCTATGTATCCACTTGTGCTGCGTACGTTGCGTTCGA + BBB@AFBFFFF4B4ECCEECEE ?EFCEHGGFCFGFHGFHHHFFGH5FGDGHGFFF2AFEEHGGFHHFGAFHBE4?EEHHFGEGEHFHHEBDH31?E30EEE/////22222?21//<//11<11<10<------../..//..--.0:000....9..-/00.9...00000000.//////-..-.:.-..-./////.---9.;........///9/////////////////-..--./9.-..... @M03555:476:000000000-CJGL9:1:1102:6998:12350 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCGGCACAGGGAGGACCCACGCGGAAAAAGGACCAGCGCAGACGCGCCAACCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATTTTTGTTTTCCTTTGTGATTACTCATTACCGTTTGCCTCCCGGTCCGTACTGCTCCGTTCTTCTGTTCTTCTCTGCTGTTCACCCTTTTGTACTTCTCT + AAAABFFFFFFFGGGGGGCCE?EC2GGD?EDHCEGEEFFGCC0FG?FEFHHHHGCEGFFFGGCEGGGGE/E?>CDHHFCC/B<FFHHHHHHG<FFHHFHHHHHHGF..FFGFFH0<CCFFD0CCFC/BDCDCHGFF0GHFB:0:G:-9--./00099;////9/9/9.////////9////;9././///...-----....///9/.;/..////////9/////////////:..////.///////; @M03555:476:000000000-CJGL9:1:1102:22686:12408 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCTCAGGGGCGGAAAGACGCGCAGGAGCAGCAGGTCGAAAAAGAGAGGACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACAGCCGCTTTGCTTGGGTACGCCAACAACGACTTATTACATATCGGAATATTGTAAACTGATGTCTTTAGGCTTGAGTCATTACGGACAGCGCGATGTGCTT + BB@AAFFFFFFFBBEGGGGG4GF4ECE ?EE?FBGGCEGGGGAFGFFHAHGFC>/11EAEFGGDDHFC/EDDC/GF?@/DGBGFFGCG/?CFFDD1GFGHFFHCFGCHFGFGE0<.CG0DGHE</CFF@ ::GGCGHHGFG.BC0:B-9@ ../..--9../////..;//..-9-....-.-../9////////.-..9//////////9///9//////////-./99/////...--..-9---../9/: @M03555:476:000000000-CJGL9:1:1102:16595:12416 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCGGCGGCACAACTGAACCGGACCAGCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACTCCTTCTTTCCTTTCTTCTTTCCTCCTTTTTTTTCCTTCCTCTATGCTCTTTTCCCTTCCTCCTTCTTCCTCCTCCTCCCTCTTTCTCCTTCCTCCTCCTTTCCCCCTCCTTCCTCCTCTTGCC + AA11ACC1F1BBFG1BFE1AAEE/E/00AA01B1FAE/AE>>>E>E/E/ECGG/>>/C0F0FC0F0/>BFGHF111FHB?//??DB>GHHH/<2FGG0</?F2FC<<0/11FFFD1011><>---.00:0000000;;0;00;<00000009/;000---/9900/00000/////:;//////-/;//-//9//////--/;-/--9B//:9//9//99//--/-/////----9-//////--////9 @M03555:476:000000000-CJGL9:1:1102:24247:12487 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCCGAGTGCAAGCACAACCCAGCAAGGGGCCGACCCCACAACTACCGCGACACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAACACAAAACTTTTTGCCAGCTCCTATGGTCTTGTCCCCATTTCTCCCTATTTCCTCCTCTACGTATTACCTCTCATATCTATTCACTATGTTTCTTCTTCTTTTT + ABABAC4DF44B4BEFG?CFEEFHFH5AGFHHFEEECEFF2GBB20AEEGGCG?EEEDCGCFCFGGGGEE?EEGEGGHGG/@EFHGHFH0GF/B2FGHHGFFDFHF/FDGHDH1<0FFC1GHHH..001F-.<C.0DDFFBCC0=0/<......:0000-0000;0///0000000000000.//0;0000//00090000/..//9..//9////////////;//////9///;////////////// @M03555:476:000000000-CJGL9:1:1102:22310:12552 1:N:0:CTGTACGTGA TACAATGTGCTCCCCCAACTTGATATTAATAACACTATAGACCACCGCCCCGAAGGGGACGAAAAATGGTTTTTAGAGAACGAGAAGACGGTTACGCAGTTTTGCCGCAAGCTGGCTGCTGAACGCCCTCTTAAGGATATTCGCGATGAGTATAATTACCCCAAAAAGAAAGGTATTAAGGATGAGTGTTCAAGATTGCTGGAGGCCTCCACTATGAAATCGCGTAGAGGCTTTGCTATTCAGCGTTTGT + BAABAFFFFFFFFFGFEGEGGGCHFGHGHHHHGHHHFHFHHHFHHGGCFGFGAEFGGGFEGEEEEFGHGFHHFGEGHGHHHG?EFEHHHGGGGGHGGGGGHHHGFFCGFGFHHHHGHFFHHHHHHGGGGHHHHHHHHHHHHHGGGCDGHHHFGHGGHFFHHHGGGCHGGGGGFBFFGFGGGCFGGGGGGFFFFGGGGGFFFFFGGFFFFFFBFFFFFFFFFFFFFFAFFFFFFFEFFFFFFFFFFFDFD. @M03555:476:000000000-CJGL9:1:1102:22685:12632 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCCAAAGGCATCGCCGGGAGAGCGGCCACGGACGCGAAGAGCAAGACCCGTCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCCTCTGCTTGAATAATAAAATACCGCATCCTTTCTCTTTTCTATATTCCCTTCTTCTCTCTTCTAACTCCTCCCCATTTCTAGTTCCTCTTTCTTGCTCCTTCCTTCCCCTGCTACCC + AA@AAFF11 @>FBGGCGGG1B0FCCCGB0EFCEG/EC//E/A/AFCC/E//>/>>/0F10FHB0?/?/?E/E///?/G//@//<F00<GFACGAFD1F1?<11?0><>CG1FD>.</.<0=00.<<<GG0:C-..00::0CH.:0090;0900090;--9?/;90;CB;0B00;900;;00/;BB/////;/;////////9/-;---BB/////:9//:/9:/BFF//;9//FFBFF//-9;-;://-- @M03555:476:000000000-CJGL9:1:1102:13969:13241 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCGCTCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAATAACACCCTATTACCATCCACCCGGAACTACTCAATAATTCACACTAGCTGTAACTATCACCTATCTGGAAGTTGTTGACTGTGTCTTGCTCATAAAGCCTATCCTAGGTACCGCTCGACTCCTGCAAGTGTTATAGACTGCGCTTGT + A3A3AFFFFFFFGGEGGGGCFFGG2EEFAFHHGGGGG33GCEBGHHGHHGHGHHHGFGHCFEEEFFFHGHHFGHBDFHGEF3ED@EEHG4GHHHHHGEGFF /03333B//?22B33?3320220/////>1111<11111111<<11<01<111111=11000/</000000//00=0..0:0:00:00000000000000.//00/900/0090.-;----9.////9//;////;///////.-99.. @M03555:476:000000000-CJGL9:1:1102:23933:13470 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCAGGGACACGTACCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGAAACTTTAGTCCTTACAGTGAATATACTTCTCCTCTTCTTACTTTCTGTCCACGTCTATCCCACCCCGTTACATGCGCCTTCGTCCACTGCTTTACACCTAATCGATTCTTTAGTACCTCGTCTCCAGTTCATC + BBBBBFFFFFFF4BEGGCEGGG2FFHDHF2FEEE?EEA2DHE0AEGBCFGFCAFCFGDGHHHHHBDGHEFEEFFHHHH1>>?E/FDGFDHH4FE/<FFGGBEFGFABFGFCC?///0000111111111111111111111>0000D000/0000=000000;0000:0.:./:00//0...--....0;00....=0./.9.//9//9///;/////9;..9/;/://///9///.9...///:///// @M03555:476:000000000-CJGL9:1:1102:17196:13550 1:N:0:CTGTACGGGA CATTCAAACGGCCTGTCTCATCATGGAAGGCGCTGAATTTACGGAAAACATTATTAATGGCGTCGAGCGTCCGGTTAAAGCCGCTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAACACTGACGTTCTTACTGACGCAGAAGAAAACGTGCGTCAAAAATTACGTGCAGAAGGAGTGATGTAATGTCTAAAGGTAAAAAACGTTCTGGCGCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTA + CBCCCFFFFBBBGGGGGGGGGGHHHHGHHGHGGGEGCHHHHHGGGGGHHGHHHHHHHHFGHGGGGGGGGGGGGGGGGHHHHHGGGGEHHHHHHHGGGGGGGHHHHHHGGGGHHHGGGGGGGHHHHHHHGHGGGHGHHHHHFHGGGGGHHHHHHGGHGGGGGGGGGEGGGGGGGGGGGGGGAEFFFFFFFFFFFFHFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFEFFFFFFFFFFFFFFDFFF=DFD< @M03555:476:000000000-CJGL9:1:1102:9491:13578 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCACGCAGCACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATCTTCTTTCTTCGTCGCTGCGAGATATGTACACGATCGATCTCTGTGGTACGTTTTGTCCGCTAGTACTCCAGATGCGTGGGTCTCTCTGTTACATCCGGTTCGCCATCCGTCTTGTCTACGAGCAGTGTTGCCTGTCGT + BBBABFCDFFFFGGCGGCCGCG?EEC2EEFEEDEGFGE?EEABFHFHHFHHFGHDGHHFHHHHGFEGHGEDHGGHAFFFGFGGEHHFDFEEHEDFBGHHHF3B3?/<///02>2222>2221/0/////.----.<=0<0000.-..-....0;000/:00.///../00.-.-.90090/;/0;00-..--..99/////9/////9.--9.--.../.9....//;///...--///9/.//99//.. @M03555:476:000000000-CJGL9:1:1102:18849:13691 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCGCAGGCCGCGGCCAACGCCGACACAGGAGGGAAGGGACAAGACGATACGACGAGAGAGCCGAAGCAGCATGGCTCTGGTGGCTCTGGCTTCCGTCCGCACGGGAGCGCACACCGGAGACCCGGATACGAAGGGCAGTGGCCACCGCGCGAGAGAGCCGCAGCAGCAGACCAAGTCTCTGCTACCGCACTGGACGTGAATTTCGTATGCTGTCTTCTGCTTTTAAAACAAAAC + A11AAFFB11B11FE1AE1E0A0F000/A/E///A//A/>//00/>F///BE?//?F000>>>FEE/??<>CC?///?//>/CB000F1AFBF0?1<0.CGHFBFGF0FGA0=.-<<<@C?CC?9-;--;.-A9--9..@ ;-A-BE--9ABEF-/;BEFF--A=;=@---;--;-;-;9@ -:9A-/A9--;9//;////9/9-----/-/--:---//9/;;BBFF/B;//;/9/9;////////---;- @M03555:476:000000000-CJGL9:1:1102:4037:13710 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCTAGGTCACAGCATGAGACCAGACGAACCAGGCAAGGAACAGAACAGGACCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGTTTCGTTTCCTTTCCCTGTTCTTTTTTACCTTCGTAGCTATTTCTCTACCCACCCTATTGTACCGCTCTTACCTCGTCCTGCTATGCCGCTCTGTCTC + AAAAAFC11C1CBFEG1EG3AFGBFFFHEC1FGFHF00F00AAAEEAEGHGCFFFECGBGFHH0FGEG>>EEEA//F@EE ?EHHHHFBG/F/>BEHHFHHHEFGE</?CFBFFAFF//2FHHFFAGEHHGCGHE1?GGDFF01110--<-<.0::0.:;:/00000000000;000009.;090/:/9..000;00000009-/:---9///////-----//:///-----//;/9///--;--/9/// @M03555:476:000000000-CJGL9:1:1102:26477:13729 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCCAAGCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATCGTTTTTCATTCGTTCACTCCTTACCCTCTCTGTTCTCATATCTGCTGTTATCTTGTCCACTATCTCTTTTCCTCGGTGTACGGTTTCTTTGCCACTGCGTGTCTCCTTCTGTATTCGCTTGTCATGCTCTGACTTCTTGCC + ABBBAF4BC4CFBGEGGEE4F4AEEE?EEGGGG?EAE33FA1ABGHAG5GHFFDGHBDF?AGABGF5F?FFE15DGHHF?CGFFEECHF4F4F4BGFCFHGE/</</01/?//??02@111 ???1111<1111001.0011<=D111=110000<0=00=0000000=00/:0:;00000000;.:..00.99.:900000900/00.:../00000////////9;.;.9/////9//////9//9/// @M03555:476:000000000-CJGL9:1:1102:16732:14123 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCGAACGACAGCAAGAAGACACCGGCCAAGGACCACGCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAATCTCCTGTTCACTCTTCACAACTCCCCTCATTCTGCTCTCGTCCTCTTCACTGTCTACATGCCCCACTCCCTTTGTCCTTACTAGGTCGTTCGCCTTCGTTCGCTATCTTACA + BBABBFFFFBFFGGGFFE?2AEGEG?EGHHHHHHBGGHDGGE0AGGEFHHHGA1@EE //??AGGGCEEHFFGFFGABGFB?DGHFBFGFGCEGFEDFGGEC?CCG22?FFGGGFG/?<CFGFHHF1<011=GFGC-C./0000/:0000000000009./90..;;.0000000000.....90000/////9///9////..../...////////////////--...-.9./../.9-.9:////;/ @M03555:476:000000000-CJGL9:1:1102:21310:14380 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCATCCGAACACAACAAAACACCGCGCACCACAACGCAACGGCCACCATGGGCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAACAAATGTCTTCTCCCATAATTTTACTGTTATTCCCATCGTTCGTCACTGGCTCCACTACCATTGACTTCTCTTAGCACTTCGTCACCATACTCCACCCCCCT + AA?A?FCDCFFFFFFCBEEDFGG?AEAEGCEH2AEGFEEGCEE0EEGG1FEE?@EE?CEGCEHFGHHGFE/E/>CEEGG/<E/BFGAFFGHE?F1FGFHFHGEGHCF/AGH?1<FCHHGHDGHC<FC?CAC<GFFDDC<<GF.A.;..0000000000/;9/0;000000000000000099.........//.../.9.;//.:////////9///////////.9...////.////...---- @M03555:476:000000000-CJGL9:1:1102:25107:14445 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCTCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGTTCTTTCTCTCAATTACGTCCTGTTTTTTCCGATCTTTTCTGTGTACTACCTGCCACCACTCACTATACTCTTTTTCCATCTTGTCTGTACTCTCTCCTTACTACCTATCTTGTTCCTTTTTCGATCTGTCATATTCTATCTTCAC + A3AAAF>@C4CCBG2ABA2AEAEAE2BFGGGGCEFGBEGGFFB1F5FFFFFGHHHHGGGGCEG5GGGHHEHFFHFG1?BGG/E@HGFGF4FGF1 @3@1EE//03B2B211222211B?211/<0//111@ @/<01/<?-0111<>1111<1111010../0<../<//00<0000;0<00:0000000;000;00;;09909000/000/0000000000000;00/-.;../////////////////9 @M03555:476:000000000-CJGL9:1:1102:26747:14751 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCACACCACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAACATACGTACCTTTGTTCCGCATCGAAACCTTGTAGTGTTATTGATTTGGCTCGCCCTTGCGATAATGCCGTGTTCCCAGTCCTGACTGGCTGCTCCCCCCCCTGCGTTCATTCTCTATATCTCGAGCCGCAACGTGCTGCTCGT + AAAAAF11BCFFFFEGGAAAB00BFC?E0ACEEGE/EEGFHFCGHFA0AEFFDGHF1EFFHE//EGF2FGEGGAF21BBGCBEFG1>?EFF2B1BGFCAG11B/////B11011//2B11>012//<///0<//00111<11011<1<111111111<--<.-///--...0000-;-:900000/00000/90..9..000...;--;------:9/////////9//-/---9----9----;/9/-9 @M03555:476:000000000-CJGL9:1:1102:19458:14867 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCCCGCCGGAACCCCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACACTCATCCCTCAACTTCAAATCGTAGTACACTATTTTGTGTGTTCAGCATACTGCAGTTACATGGATTTTCACTATATGTAAGTCGTCTAGAGGAGTGCGCGGACACCGATGCCTACACGTTGAGCTTGCTTGG + A1AAAFFF1CFFGG1BFEGGGCEGGC/EFGGEGGGGGFAHFE>>EEGFGCFFGHHHHHHHHHEGHHHFEEGGHHGBGGHGHEGE2BGFCGHHHECCHGHFFFGDG0BFF0////<<.0100111.0/.0/00000000.....00;00:00000;//..90000000;0000/0;00000/000090000;0000090000-9---///-----/------9-9-9---///-/------/////-///- @M03555:476:000000000-CJGL9:1:1102:7200:14971 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCGCCGAGAAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATAATGGGAGCAACGAATGTAAGTCCTACCGAACGGGCGCCTCTCCTCCATCGTTTGTACTCTTGGTCCCGTTTCTGTGCTTCAGCCTCCAACGTCGCACCCTACGTGCGTTCGTTTCTGCCTAAATAAGACGCAGTATATATTTCAAA + AA?ABFBFFFFFGBEBGGGGEEA222EGFGCCGEFAA113BA211GFHEF5FHHGGEFFECBD@EFBF1BE44FC ?1F3GBEACAFGB4BG?30??3BEE/>01B22100//?00////111111111110/.---..---;..///0///00.;.../00900000090.-..//00000;00000///..9/...-----.;......-..9.././//////////9/9/.---.////////9/// @M03555:476:000000000-CJGL9:1:1102:25520:15029 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCGCCCACACGTAGACGGGCCCCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATGTTTCGTATCACTACACTAAATTCTGTAGATGACTTGGAGTGTACGGATTCGTGTGGAGGCGACGATATCCATGTAAGTATGTCTGCTCTTGTAGCTGAAGATCTTGAGTTAGTCAGTCATCGT + AAABBFFF4FFF4GCGGGGGCEEFGGGHHHCGFG?FEEEGGEF0EFEHGE?CFGHEHFHEGHFHFHHHGHGGDGHHGGGGHHHFHHBCGCGBHGA?/B?GHGD@FFBG2BDGH0 @GFHGCFG<--<00000..//.0000//:0/:<000;0000;0090;9000//.000.....9./....9...-----..-//////////////;/////////9/9////////;/////////////99/9/. @M03555:476:000000000-CJGL9:1:1102:7501:15264 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCCGACGGGCAACCGACGACCAGCAACAAGGGACGGGCAGGAAAGGCAGCCTCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGTTTTAGTATGCTTGCATGTGTCAGTATAATGTGTATGTATTGCATATCGTCTCAGTGTGGCGGTATGGGTACTGGTCTAGATGTGTGTAGTCGTGGG + BBBBBFFFFFFFGGGGGGGGGGGGGG?EGGGGGGGGEEFFHHHHFGFCEFGGCGGGGFHHHHHHGGFHHGAEF@EEA ?C@D @DFGHGHFFGFEHHHHGHHHHGHHGFADHHGGGE>FEFG1>FECGGHGFD.<GGFGHEHGH.:C0:C?-9-.00000/90000900.///;//////////////;//////9/;://9/.....//:///.9---..;..://://////////;/9../;//.;..- @M03555:476:000000000-CJGL9:1:1102:27893:15299 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCACGGCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATTAAAGAACCGGCTTCATGTTTATTAATTCGCGCCTGCAATCTCCATCTATTTATCCGTGTTAATACCATCCAGCCGCACCATGTTGAGATTTATCTCTGTCCTTTATCCATTCGCCATGTTTCTCTTCTAACGAGCGTCATAT + A3AAFCBBFB4BFEFGCA4EE?EE2222C2F2A0A0A3AG1EGFAEG1BBEFDGFDFEBGFFEGFFB41B2?FE4BE??>FFF3>?EG/FFGFDFC033?0//>>0111?1100//////01111111>111111..---.../00000000:0<0000000.....00000000;.00/.---9-.000000/.//////;/////////9////;///...9////;/;///;///...--....// @M03555:476:000000000-CJGL9:1:1102:20766:15353 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATAATTCCTTCTATCTTTTTCCTCCCCGCTTTACTTCTTTCTTATCTTCTCTCTCGTCCTTCGATCTCTGTGCATCTATTCCTTCTGCACGCTTCGCCATCAGTCTTCCGTGGCCTTGTTGTGAGCTTGTGCCTCCTTCGCATTCCTCGC + A>1A@ 1C1CFGGEGGE1E0A0EAECG?EEE?FFG0A0F/EAGBFFA21AG1FHGEEEEHFFFHCGAFF1FHHF?FEBGEAEEE2FBFGHFABD1B/@E<01122212BBB222122>101110/////</111111<11111<=<11111111..<..00..=.<0000000000000000;00000/.-..99..../09000000.09--.9//9/-;-////9/9///9/-;//--;--////-- @M03555:476:000000000-CJGL9:1:1102:11894:15381 1:N:0:CTGTACGTGA TGGTAAAGGACTTCTTGAAGGTACGTTGCAGGCTGGCACTTCTGCCGTTTCTGATAAGTTGCTTGATTTGGTTGGACTTGGTGGCAAGTCTGCCGCTGATAAAGGAAAGGATACTCGTGATTATCTTGCTGCTGCATTTCCTGAGCTTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTCCTCTGCTGGTATGGTTGACGCCGGATTTGAGAATCAAAAAGAGCTTACTAAAATGCAACTGGACAATCAG + ABBAAFFFFFFFGGGGGFGGGGHHGHHHGGHHGHHGGHHHHHHHHHGGGHHHHGGFGHHHHHHHHFHHHHHHHGGHHHHHHHGGHHHGHHHHHHGGGGGHFHHHHHHHHHHHHHHHGHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHGCGGGGGGGGGHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHGHHGGGGG:EGHGCGEGFFFFGGGGGGGGGGGFGGGGGGGGGGGGGFFFFFFF0 @M03555:476:000000000-CJGL9:1:1102:5904:15557 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCCCAGCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATCCTTTTCATTCCCTAGTTCCTAATATAATCTCAACATGTCTAATGTCGTTATCTTTCAAGTTAGTCCGTCGTCTTCACATCATTATTGACACACACAGTGGACCCATCACAGTTCCTTCACTACTACTTGCTCGTGTCGTC + A>>AACCDCFFFGGGGGGGGFEFGG?F?EEEGHGGEFGHHHFFHHHBCGGHH5FDFHHHHGGFDGFHDGHHBGAF4FFGGGHF4GGEGHGDGGFHGFFFGH0<<//>/02222><21?1111<<01111111?1>1<1<11110<01>111110<0.<<..000000000000000.:.--...00000009;0;09;000/...;.00/0//..////://///////////;/////9//.9...... @M03555:476:000000000-CJGL9:1:1102:24524:15829 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCGCGGAAGACACAACGGAGCAGAACCACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAGATGACGATCACAGTATAGATAAAAGCTCAGAATGTAGGAGTAGAGATGACGAAACCTCAAAACTGATGTACAACTAGGATCGTAGGATGGGTTGCAGGGTATGGTAAGAATTGAATG + BBBBBBFDCFFFGGFGGGGGGGFGFF33GCCEGGFGCGHFCGAGGGEEDFF?1FEEFEEFFFFFHHHGHHHHGHHEFHFFHCCGGHHHHHEFFAF44BFFFFHGHFGACHGHHHGGHHFFHFHG??<-----..0000...../00009900000000.000/000000000///0;//////..-.-.9/////.//////////////////..9..///9.;.-.//.-.///.9//////////// @M03555:476:000000000-CJGL9:1:1102:7779:16008 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCGCCGCAGCCCAAACCTGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGCACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAACATTCCCTTTCTCTATTTGCTCTGTCTTCCTTTTTCCTTCTTCTTCCTCTTCCCTTTCTCTTTTACCTTCTTCCCTCTGTCCTCTCCGCCTGCTTTGTCCTTCCTTCTTCTCTTCTTTTCCTTCCTCTTCCTTCC + A>1>FFFFBFFFG1FGEE00AAEA?EEHCHHC0B0BGB//GGEECECBGG000>/GA0BDG1FBGF1@E B>/>F11FFE//?@B@>FFGFGF1EE@GEF21FEG1B11 <B///?/1112?1?1?1111?111?11111111<1111110111>10=0000<0000=/=0000<000000000/0000/;//00:0000//....../000;00000/00;9000000;/99///9//9//;:////// @M03555:476:000000000-CJGL9:1:1102:25339:16354 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCACCAGGGACACCAGAGGCGCCGGAAGGCGACGGCAAGGCAGCCGACCAGACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATACTATCTGCAAATTACAAACAGACTATCCGCTACGTGTTACTTGTCACTTTGTCGCAAACACTCTTATCCTTGTCTGATCTGTGTCGTAAACGTCGA + AAAAAFBB11BFFG1GA11BCFEF00FAF0GH000BEEE/E/A//A//EEE//>F//0//F/>EE?FHG//<///<<FC/B@CFFHHGHGHE /FFFGFHHHBGGHCE<AED<11.GGC.=0=0CECF/0D@:-.CGCFFFGC.;CGA@9-- ;.000000//;;/9//////9---9-/////--------/-/////:;//;////;---9-9--///9////9;//9///////////-----/--;9- @M03555:476:000000000-CJGL9:1:1102:25308:16554 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCAAAAACAACACGACATGCCACTTCACCCCCCGCGCGGCGACGCAACCACACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCTTCTTCTGCTTGAAAAATAACTTTCCTTTTCCATTCTCTTGACTTTTTCACCTGCTTATCTCTTATCCCTTCCACCCCACTCGCCTGCTACTTTATGTTTCCCTCCTTCCCCTACTTCTC + A1>AACFB1CCF1BEGF?F1D00EE0ACF0A?E01BB1AB1BD1FHCAE//>EE////>/>/<ECE/<B<<B/B/</FB///<FHGCFCFA0GGDF1FGEEFFFF-C.CA0D00.</..;0:;..::/000<0<;0;09BFB.009..00000009;CFB0009;;000;00;;000;//////-////9//;//;B//:/;9//9-9---/-99---///;/////;//;///-;;///:9:--///// @M03555:476:000000000-CJGL9:1:1102:28345:16634 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCACACCCCCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACTGTCCGATAGATCTTATGAACGTTTCATCACGAGGTTATGTGTTACTTCATTGTACTCCATTGACACTCCTACGCTAACTGTGGCCGTTCATTGGCGCATATCATGGTTAGCTTAGCTAGTCTACTCTTTCTATTGTT + AB?A3FF@CC4B4BA4F ?EFGEFGGCEAEAEFEEHGGGGGHGCHHHFHBGCGHGEHEFGFH2>?GGGHFGHGHGHGD4BG1?EGFFHG?@A?FGF4GGC0FD3?C?>/////11<11...-<00000000000..///00000..--./00000;/00000000000000//00000000//900.-...///;/...-99./;////-.-..///////;/./////////////////;////9//// @M03555:476:000000000-CJGL9:1:1102:28685:17352 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCGAAGCGGAAGGAAAGGGGGCACACGGGGTCAAGCGCACACCAAAGCCCCACGAGAGAGCCGAAGCAGCAGACCACGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGTAAAAAAACTACCTATTTCCTTTCTTTTATCTATATTCCCTTCTTCTCTATTCTTCTTCCCCCCCCTTTCTTCTTCCTCTTTCTGTACCTCTCTTTCCTCTTTTTTCC + 1>AACC11CFFAG?GFEE0AEGG?00EEAGG0BAEGGGCCGGGGCG/FGHBEGEEEFGGAGHBGHE?EGCE/E/EHGGG/@/F0FGHE0C<////?FGDGHFHCCF<AFFGFF.<0..1>FHFFEC/DDCGC..=0GFFCC.;:00;--.00//000009;0C90;09009;C0;000;00;099;0009/9/9B/9//9/----9-99/BB///////;////:9///://////////999///-// @M03555:476:000000000-CJGL9:1:1102:7823:17784 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCCGCTCCGATCGCACCACCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAATTCCTATTTATCTGCATTACCCTACACTATTCCCCATTATGCGCATTGCATCTATTCTATACTCTCATGGCAGCCTCCACCCCATTCATCCTCTCAGAGCTGTTTCACAGCCTATTTGTTGCTTTT + A@AABFBDFFB4AFEFGGEGCG2EECFGG ?2AA2BAEEEAAD?AFEEC?ECFG1FGHFBGGFHGHGHFFHFF?FGGHFHFGFBCH/44BFCCE3FDDEEGHFGFFFDDF?GHFF//>@/-----./000000000:000000000/0/9//0/00000/9/00000..-..000;0000000////////9///./..........-../://///;////;9/////9///9/.../////9..///// @M03555:476:000000000-CJGL9:1:1102:8255:18146 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCCACCGCGGAGGACGCGCTCGGGCCGGCAAGGGAAGAGGCGAACCACAGCACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAACAAAAACCTTCTCCTTACTTGTGTCTGTCGTCGCTGCTACTCGTTCGACTGGTCCCTTGCGCTACGTGTATACTTTTTTTATGCCGCTTTACTCTTCGTTTA + BAABBFCBBFFFGGEGGGFBFEGGGGCGGCFEGGGGGCEFCEGGGCGGGGEFGGHHGEEFFGHGHHHHHGDCDFFGHHGCGCCHFGHHHGCGEAHFHHHHGGGDHGEEGFD<DHC/F.EG0CGAEGHGHHGDGFFFFFEFFB.CB0.;......:.9//9//////9////;/////.9.9-.../////..9.....;.9//.//.9--9--.;.9.////;//9--9///9---..///9///.9/.. @M03555:476:000000000-CJGL9:1:1102:13216:18490 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCAGCAAAGTCCGAGAGAGCCGTAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAACAATATCTCCCTCCTTCACTCCTCCTTCCTCTCTCCTCCTTCCTTTTCTTTTTCCTTCTCCCTTTCCCTTCTCCCCTTCTTCCTCTCTTTTCCTCTCCTTCTCTTCTCTTCTCTCTTTCCCTCTCTTCCTCCTCTCTCTCTTCT + AAAAABBB1BFFGF1FGGCGFG01BGGDFA000FECGG/AECEACF00AACC0FGDG21EA1AGEFFGHGHHG/B0/BFFF1FFFF02F/EEHGFHEEG1F01>1B///1122221000/<<1112<<D0B01101110110@00 @1101221111<1101111111?<111111>01110.0./0=00000/0<0000000//0:00000<:00000000000;00;///00;00;///0/00000;00 @M03555:476:000000000-CJGL9:1:1102:10818:18757 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCCGGGGCACACCGGTAGCGCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACTGCGCTTTGAACAAAGATGTAAGTGCATCAGCCTGAAGTCCGTTAATGATTACTACGCGTCGTCTAGTGTTCCAACCATCGATTGAAGTTAATACTCATACAGATTTGTAGATGACTTCTAGTCACCT + BAB@AFF4CFFFFGFGFCGGGGGCEDCGFGG2AEF?AEAFFEF?GHC0>@EGBCHHHGEFGF@F4B @FBGFGCEFEGHFEGGHHFAFF4?FG/BFCG2CADFGEGF2GFC0CB@CCGF ?--.000.--..00000/.//000:;000000;009//.900;00.;09.0000000;00.--9--;...////;/////..//./././;//////////:////////9/;/////////9///9/9//9 @M03555:476:000000000-CJGL9:1:1102:26867:19062 1:N:0:CTGTCCGTGA GTGCCAGCAGCCGCGGTAATACGAAGGGACCTAGCGTAGTTCGGAATTACTGGGCTTAAAGAGTTCGTAGGTGGTTGAAAAAGTTGGTGGTGAAATCCCAGAGCTTAACTCTGGAACTACCATCAAAACTTTTCAGCTAGAGTATGATAGAGGAAAGCAGAATTTCTAGTGTAGAGGTGAAATTCGTAGATATTAGAAAGAATACCAATTGCGAAGGCAGCTTTCTGGATCATTACTGACACTGAGGAAC + CCBBCFFCFFFBGGGGGGGGGGHGFGGGHGGHHHHGFGGGHGGGGGGHHHHHGHHHHHFHHHHHHHGEHGGGHGGGGHHHGHFHHGHEGDGGG?BBGFHHHGHHHHHHFHHHHHHHGHHHHHHHHHHHHHHHHHHHHHHHHHHFHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHHHGHHHHGHHGHHGGGHGGHHGHGHHHHHGHCCHHHFGCGFGGGGGGGGGGGGGGGGBFGGGGFFFFFFFFFFFF @M03555:476:000000000-CJGL9:1:1102:16446:19126 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCCGCAGACTGACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACACGTTTACTTTGGCTTTGAATATAATGCTTTCTTCTGCCTGCGTTATGCTGTATAATGTCTGGGGGCGACGTGTCTGTCTTGCATGTCTTGTCCCTTGCTCCATAGTCTGTGTCTGTATCACCTGTCTCGTGTTGTC + BB@BBFFFFFF @4AEFGFGCGGFCHFHBFFGGCEFGFFGCFEGHHHHGFFHGCGHHGGHFGHGHHGGFFHFGGFCGHGEGFBGF?EEFFFFGGFGFH44GGGAGBFHCC/@<//0/00?/1<1@101100111

<111111111111>1<=0000.-<..:0000;0;000;0000//--------././//;/;/9/9////9/////9/.//////////9/:/;/9F//////.;/9////9.99../ @M03555:476:000000000-CJGL9:1:1102:12049:19557 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCCAAAGCAACAGCCGCGACGGAAGAGCAAGACGAACAGCGCCGCACAAACCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGAAAGAGGAAGAACACAGCAGACGCGTGACTGCGCTGTAGAGTAGGGGATAACAGAAGAGGCAACAAGAGTGGACGGGTAATAAGTGTACGCAACG + ABABABBDFC4>FBECAECFFACFH4BGHFGECGGGCCEDGCABHHGFFFCGGGHH?CCGGGGGGAACE/@EECFDFFF?CGGFFHHFFHHEFGGFHGHHHHHGF@FADFB GFGFBEEHBFGFCGHF=DC<AAFF4CCFFBBEGGFEGGG2AHBEEFC2BBFGA2E2EGAFFC0AE0CFFFCFFGBGGFHFHFB3BGHEE?CEHG@FBAGFCFBBGEEFFHGBGCE @GFHDEHHGHCCGF0//////<1111<10<10<11<1F1111.000<0<.---...;/00:00000000/./0.:/0/000900.....0000000000...///9///////////////9///9///..9./9./..---.9.;9//... @M03555:476:000000000-CJGL9:1:1102:14305:20752 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCCACGGCAGATCGACCAAGCTGCCAGGCCCGCCAACGCGGAACCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTCCTGTACGTGCATATCGTATGCCGTCTTCTGCTTGAAATAAAATTACATTCATGTTGTTATTCTTTCAGTTTTCTCACTTTTCTGCTTCCTGCTCTTCTCTCCTCCCTCTGTCGCATTTCGTCACTTTTGTCCACCCTTCCGTTCCTTTTC + AAA11CF1@1CBFFEGAE ?1FE000A0F1B0/A//FE11F01B//F/AEE?/FEFE??@E>//>>///?0E<GDH>GFG///??D<011222@G22221101111111111111100--- ..10000AAAFFAFFFAGGGGGGFGGGHGEEGGGGGGGHHGHHHGHHGG1?FHBFHGHHHGGGGHHGFGGGGFGGCGDG@BFGFDD ?GHHHEFHGDGHFFGHHHFGCGAHHDDDGHHGGDGGHHHHHHHFHHHHHGHHHHHHHHFFGGGGGFDEGFFGGEFGGGGGGGGGGGGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFBFFFFFFFFFFFFFFFFCFFEFFBFFFFFFFFFFFFFFFFFBFFFFFFFFFFF @M03555:476:000000000-CJGL9:1:1102:12733:21216 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCCAGCAGGCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAACACATGTTTCGCTTTATAATTATATATAGTTGCTTGCCGTTATTCGATGGTTTATCCCTACGATTATCGCTTATCCGCTTATCGCTCTCGCTGTATTCTTGTTTTTTACCTTCTGATCTGTATGGTGCCTATTTTCTTCTCATT + BBABBFFBFFFF4FCGGGGBFGFGHEGG?2AE2FDHEE0AECBGCGFFEFHGGHHFHFGFFHFGGGGGHFHF1BGE1F4GFEE>B3@FEE ?GFBDD4FGB0??3B>//0/223333?//??32@222222222222@2@221 /1/////110111<<101=11111......=0.....000:....;;.::..0....9000000000//.-;000;////////////.;/////9//;///////// @M03555:476:000000000-CJGL9:1:1102:15990:21520 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCAAGCCCGCAGAGCGCCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATACGCCTCCCCATGTATATCTAGCGTATTTGAGTGTTCAGGTGGAGTTCTGGTAGCGCGGTGCTGATCGTCTACATCAGAAGGCACCCCTCTGACGCAGTCATCTAATTGGCCAATAACTGTCTCTGTGGCCC + AAAAAFF1C1FCGGG111A11FA0A0E00FE0E/AA/AA////0A/A/>G0B0BG0?GH01B2121@G @1B/?//F2FG2FHH/>212>>FCE021/EE0/2B1B1B1<1110BCG/011?/>F.<>.0<011><111000-...010=00/00000000=/<00000./:.:0;;../0.00090../.-/0;0..-..00000000000;0000;/////-9/9/9/-//9/;----// @M03555:476:000000000-CJGL9:1:1102:11792:22643 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCGGAGACACGCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGGAAAAAAAAATAGTCTTTTCATTGGTTATCGATTAATAATTAATACTAGGATTTTGTCGAATTTGTTCTATATTTTGATATTGGTAGTTTCGTTCTACTAGTGACGGTGGTGTCGTAGTTAATCTTGTACGGACGTTGTCTAGTCTTCG + AAAAAFF11B1C1FEGGGC000A0BEEAAAEE?FACFA/A/EBBBF00>>F/GFFHFGD1GFHHEF/EGGFFFHGGC/2FGGHAEHE2FEEGHGG2F11BF/11B00////01?11111<11<1111/111...1.111=>1111=0000/0<0000/0<..:.;00/0<0<000;;0.000;:0;009;00../.;00000;000.-9.99../-----/////:;////--------;//////9//- @M03555:476:000000000-CJGL9:1:1102:25889:22850 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCTCGGACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGCAAAAAAGTAAACTATCACAGTTTGAAATTTCAAAATACAGTGTGACATATATTGAGCGCCACTCCCACAAGTCTATACGTACTGAGTCTGTGCCACATGGAGTTTCTAAGTTGCTACTGGATCCTCACCCGTGTGCCCTTGTAGCGTCGATA + BBBABFFFFFFFFF?4GEEGGF2EEAEFGFEGGC2AEEBBCFHFGHH1FHHHHHEGFHGC@ ?FFFHHFFHBCFCFGHDGFEHHGGGE/FFGHF4FGE/?43??//34441B12B2211122111121@ @22><11111212111111?1<111?1/>-.<1>0.00.<.0000000.<0..00:00000<000:.C0/0///00000009;B00000//09/000...-....00///.9//:-.9--.. @M03555:476:000000000-CJGL9:1:1102:9997:23640 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCAGAAGCGCAGAGGCGAGGGACCACCGGGGCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATATCGTATGCCGTCTTCTGCTTGAAAAAACAACACTCCGCTCTTGACTATTACTTAAGACTACCAGTGCATCTTCTCTTACCAATTATTCACCTCCAGCTCACGCCTCCATTTACCCCTGACCTCTTTTTGATTCGTACACGTGTATTGTAT + BBAAAC>44CCC4GEGG?EC4FH4AAAA2FFEGGGGCGGCFHCEECEEE?/>>FEGHEEEEGHFGHHGF/B/GHHGHHHGGFGHGGHGHHHFHEFC//11<1?FFG<1<@ECHGGHBFDGGCCF0.-<:....0//...-.0000000000000000090//00900900000000000/0//;////9///.9./////:...9...;//////..9.///////9/-.///./.../.9.9.;///// @M03555:476:000000000-CJGL9:1:1102:26396:24009 1:N:0:CTGTACGTGA CTTTAGCGTTAAGGTACTGAATCTCTTTAGTCGCAGTAGGCGGAAAACGAACAAGCGCAAGAGTAAACATAGTGCCATGCTCAGGAACAAAGAAACGCGGCACAGAATGTTTATAGGTCTGTTGAACACGACCAGAAAACTGGCCTAACGACGTTTGGTCAGTTCCATCAACATCATAGCCAGATGCCCAGAGATTAGAGCGCATGACAAGTAAAGGACGGTTGTCAGCGTCATAAGAGGTTTTACCTCC + 1AAAABD1A>>1BBB3FFGF1FFGFHHHHGBGEEEEGFHGFGGGEGBHCEEEEFBAEEGGEFHGE1FEDHBGGHF1@FGBFHD001 FBC0FGGFFA?E/ECB<01G1FFGFGFHBHHFDCHHGHBGBFC/CCCHFC1>GHHEAGHHBCCE@CGCGGEHHGHGGCGHHCB0 :C9;BFBFE90C.BFBBF9A9.C0CBBBFG@-@--F///;BBFFFFFF@ @BE99;/FBBBBFFB/BB9A:BFEFB/BFE @M03555:476:000000000-CJGL9:1:1102:10153:24102 1:N:0:CTGTACGTAA ACCTTTAGCATCAACAGGCCACAACCAACCAGAACGTGAAAAAGCGTCCTGCGTGTAGCGAACTGCGATGGGCATACTGTAACCATAAGGCCACGTATTTTGCAAGCTATTTAACTGGCGGCGATTGCGTACCCGACGACCAAAATTAGGGTCAACGCTACCTGTAGGAAGTGTCCGCATAAAGTGCACCGCATGGAAATGAAGACGGCCATTAGCTGTACCATACTCAGGCACACAAAAATACTGATAG + 3ABBBFFFFFFFCFGGGGGGGGHGEGFHGGGHHFHGHHHGCHHGHGGGGGHHGGGGHGHGGGGHHHGGGGFHHHHHHHHHHHHHHHHEGHHHGGGHHHHHHHHFDHHHGHHHHFHHHHHGFGGGGHGHGGGGGHGGGGCGGGHHHHHHCGHHHHHHGGGGGGGGGBFGGBFFGGGGGGGGFFFFFFFFFFFFAFFFFFFFFFFF/FFFFFABFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFBBFF/ @M03555:476:000000000-CJGL9:1:1102:10133:24116 1:N:0:CTGTACGTGA TGGCTTTGGCTTCCGTCCGGCCGCAGCGAAGGCGGGATAAGCGAAGAACCAGCACAGGCCAAAAGGGACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACAATCTCTTTCTTTATGAATAGTGCTCATCATGTTATCCGTATGACAATGTATACGTCAATTCGCCTTAGTGTACTCCAGACGTCAACATTAATACTTG + A>A?A11111@C1BAA11E ?AE0000//A//AGC/?//BFDD>E/>>0E>0/FC>>0/C/FG0/F/EGAECB?/F/FAC>//@CF0CG0FGFC0/////-01111111111=0000=000/..<..00000/0000.///:::0000000;0000000000099//.0099B009//;9//..-./////////9///////9..--.;.//////./:////9/;//.;//9//// @M03555:476:000000000-CJGL9:1:1101:9719:2806 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCCACCCAGGGCACACAGGAGGACCCCAGCAACCCTAAAGGAGGAAGAGCCACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATTTCTACTGTCTAGAAGGAGAGAAAGCAGTGTGTGGTCGCTTTGTAGCCCTTCAGGCACGCAGCTCATACTATTGGCTCGGTGCTGCTGAGATTTG + CCBCCFFFFFFFCGEGGGGGGGGGHGGGGFHGGHGAFGGHHGGGGHFGHFGHHGHGHHEEEHGHGFHHHGFCEEEFHHFECCGHGHHHHGHHHGGFHHFHHHHGF@FGFFHFFHGHHFAG3FGH ?DHGFGGC?GEHHHHHHG0?1DAC@ ----;./000000000000000......//////9/00:..:0--..:B/////////////...----//////////:////--;.-//////./;/// @M03555:476:000000000-CJGL9:1:1101:17722:3693 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCGAAACCCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAGTCTTTGATGAGACACATTTTCTGCTCGATGCGTACCTTTTATTGCTGCCTTTTCAACTCGTCTCTGGCGTGTGGGACACTGTGCTGACGCGAGCGTGTTTCCTCGGTCCACGTGTCTTCGAGTGTGTCCTGATGCGT + ABBB3FFFFFFFFGEGGGGCEEFHGFFEGCGGGEHG?EEFFHHHFFGGHEHHGHHHHHHHHHGFHGHHHHHHHHHEHBHHH1EHHGHGGGHGFE4FHHHGBGHG////>1C1@F1FFBGGAFAEGAE0E00EEECEGCHF0FF0AFG /FGGHBGBGGHGHGGHGHF2FGEFG/?G1BFC/>B021@ />FFBGHHFHB>112BFFBEB2202///BB11212BB11BB002<10//00/11121111111?1110000111111=1111=1<11111>111100000000/000000==000000/::;.0/...-.0009/9/;000;00900000009/09.//;0000;/;/- @M03555:476:000000000-CJGL9:1:1101:21945:4229 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCAAGGCAACGACAACAGGGCGCGGCCCTAGACCGACGACGACACAAAGGGTCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACTAGTAGCCATATATTGTTGGAGTGATACTCCTTGCGTCCTTAGAGTCCCCCCATGTGCGAAGGGTCCACACTAGAACGTACCTTATGTTCAGGGCC + ABBB?FFBBCFFGGGGGGG5AAFFHHEDG2ACGHHGGGGGGGGGHHHBGFEEEEGGGGGGGHGA?BEHGGE@CDEGHGC @CCCB0B0?<0GBGAF1FGH1F1DF<@////>/?1111111111..-..../0<000</?1?1.011111=1111>>111<../.0=0000<00000000000<-:..../.;/000009;C0000;000;:B0.9::;../.0009/9.....///////;9.9F///;9B..//:// @M03555:476:000000000-CJGL9:1:1101:7510:5270 1:N:0:CTGTACGTGA TGGCCCTGGCTTCCGTCCCACCGACCCCGCGAGAGAGCCGAAGCAGCTGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGCTAAAAAAAATCTCTTCCATCAACTCTTTTCAGCTATCTTCTTATCTCTTAACGCTGACTTTCTAGTTTCTGGTTGCCCTTCGTCTATTTTCGACTTCTACCCCATTGCGCTGTTTGCTTTCTTGATCGTGTCTGCCGCTGCATAAC + AAAA1>A?1>>C1GGGF1E1FFGCG?EAEE//A///E/0E/A>/@000B@0B@?0B1B1EG11F1BE//>FGHE@ /FCEFF1G1>//BE2B@ />BFGD11>1F/1BBF11///01111?<1>GFH111>>11>>11<<0=0000=0==.<.../0;:G0;000<;000:;..99;/:./.;.;0;00-...;0000;.9./9//----9////:9///BB///-9---9///-;9--////9 @M03555:476:000000000-CJGL9:1:1101:9289:5390 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCCGAGAGGTCAGCCTGCCGCGACCCGCCCAGCACCCACGCGGCCGCACAGACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATGTCGTACAGCTACTGTACTAAAAACTATACCGTAGTCTGAAAAGTCGAGTTACTGAATCATGTGATCAGTCTTAACGCTTGAGCTGTTGGTGATAC + BBABBFFFFBBCBFEGGGGGGGGGGHBGGGHHFFHEECEEGGGGGGGHHHHHEGGGGE?CEGGEGGFEHGGCGCEDFHCC?@CHFFHHHHHEHGFBGHHGHGHFFGFCFEHHHHEHH..DFFHHHG0EGFHEEGHGHHHHGFFCGHGHGCF/><--.00=00000/.0000<0=000;0;000/0///0000;0:C-----.:/090000000000000000./9..///9///...../;//////.-..9/..:..//9./;/9/9/9B//9//9//:../:-. @M03555:476:000000000-CJGL9:1:1101:20011:5687 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCGTCCCACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACCCTTGCCCTGCCTTTAAAACTGTTAGTCTTGCGATCGCGAGTGGTGAGTGGCCTTCCACGTGTCGATGTGAAATTCGTCGTTCTTTGTATGACCCCCCTTTGCTGCCTCTGCTCACTGTCTCGCTACTGACGCTGAGGTCC + A1A1>CF1BCCFFGGFGGEGGG?1GE?E0E00C0/////10B0FGH/0F0F1FEDG1EGHF?F/EEGHHEH0F/BF1@F0B /B02BE?/BFGF1FF110B110////////0?011@ @0?01->F-.<-<-;.<.<:C0:..:C0/;09C.9/..9;:C000;;BABA@ -;.;9BB/////99-;----;/////-/;BBFFBBFB//;B9/---@F///-99----/-9 @M03555:476:000000000-CJGL9:1:1101:20081:5711 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACTACTATTTATTTTTATTTTTGCCATTACTTGTACACGTGTGATCCTTCTCGTCAGTTTTATTTGTTACCTGTACCCCTTTATCGCTACTTATGTGTGCCTCACTATGTTTTTGCTCTTTATTTATCACTCTCTCTTCTTCTTGCT + CCBCCFFFFFFFGGGGGEGGGGGGGAEHGGGGGGHHHHHHHGHHAFGHHHHHHFGGFGHGHHHEHHHHHGHFHGHGGHHFHCFEHFHHHEGHH?F3DEFF?E<1/11111<1?111111<<0.<.11<111110.....<000<00000/00000;0000...:0;:....-00000900.000:/9090;000/.-00000://////://////:///;/9/:9/// @M03555:476:000000000-CJGL9:1:1101:22096:5851 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCGCACGAAGTGGCCCCCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAATGTCTAAGAAAGTAAAACCTCTAGAGATTGTAGAATTGCACAAATTGTGATAGTGTCTAGTATGTTATCATCATCCATCGGTTACAGTAAGCGAGTATGATGATGTCGAGCTTACAGAAGCGTTGGG + BC@ABFFFFFFFGGGGGGGGGGGGGGGHHGHHGGEGGGCFDFGHGGGCEGFHHFFHHHHGHHHHHFGHFHHGEEEHHHGHECG ?FG?FGFFGHHFHGFGHGFHGGHHHECDF?/@/A?<-...<000000000//000000.//000000000;00000000000/.90000000000;0000000000000000000////.....//;:////-.--./////9////9::..///://///-..-.- @M03555:476:000000000-CJGL9:1:1101:18560:5961 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATTGACTCTTTCCCTTCCATCACAACTTTTCAGCTATAGTATTAGACATGAAATCATCTTTTCTATTTTATAGTTGAAATTCGTATATATTATAAAGAATCCCAATTTCGATTGCACCTTTCTGTCTCATTACTCTCTCTTAGTAAC + BBBAAFFBCFFFGGGGF2E4AEEECAEA2AE2AEGHCFHB1F2AE5DFGFFHH5F2EFEEGFD551BFGAG5F@FG1FFFHCG @HFBGHHGHGFB3BB@FCD//0<22?>?2222@<@22<1@DG11 ?C11FD11<1<==G1>1<11111>0000000===0/////0223222@22 <22222@2 @<<1111?11111<.<>=100EG02FEB>G/2F1@BFF /FDE>///E//?111011?0101<11//0?/1111111111...--<.//FFC?C/CF0?FGHFHBG0=FDGHFHFEG0@C
drivenbyentropy commented 4 years ago

Try providing the 3' primer as its reverse complement, i.e. CGAGAGAGCCGAAGCAGC. Since these are rather long reads, you might also try to use the forward reads only and see if that increases your overall yield.

samoudj commented 4 years ago

hello my aptamer sequence contain 2 fixed region of 18 nucleotide and a random region of 50 nucleotide: The sequence is: TGGCTCTGGCTTCCGTCC-N50-CGAGAGAGCCGAACCAGC so in the program i put the forward primer in (5' primer) witch is TGGCTCTGGCTTCCGTCC and the reverse primer in (3' primer) CTGCTTCGGCTCTCTCG (Do you think it is the good way to put the amorce ??) i try also a fastaq filed witch have the two fastaq R1 et R2 paired and it does'nt work

Thank you, Samia

Le mar. 20 août 2019 à 12:43, djerroud samia djerroudsamia@gmail.com a écrit :

these are my primers: Forward primer TGGCTCTGGCTTCCGTCC

Reverse primer GCTGCTTCGGCTCTCTCG

this is an example of one my fastq files (R1):

@M03555:476:000000000-CJGL9:1:1102:16972:2053 1:N:0:CTGTACGTGA

TGGCCTGGCTTCCGTCCACCAGCCCCGACGAGTGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATTTTTGCATCGATTCCTCAAATGTGCAACCAAACTCGCACTAGTTGGTCCCGTGTTAGTACTGCAGACTGACACGTACATGTGATGTTGTGGTTATATAGATTATTATAGTTTCTTACACTGTGCTTGTTGTGTACTA + AA@AAF111>11BAB1EA100BAA0AEEE?/E//DF1GE/A/A1ACF0BFHHHFFGFGHHG1FEFEBFEGHG@E /00?/2BF1GF/B0>1/>/0?>F1BGGF01BB0/////011?1<1111/0/11111111111111110...<00<----00000/.00.-:...00000;000/9./0//00../..;0;0;000009.-999/////////9//////:;////9/9//9/:///-;----//// @M03555:476:000000000-CJGL9:1:1102:21633:2759 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCAGCAGGGGGAAAGGACAACGGACACCCAGCGCCCCCGCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATGCATTTATTATTCAATACTACATAACGTGACATTGTCGGCTTAGTTTGCACATAGTCTCTTGATTTGAACTACCCTCTATAGTTGCTACCACGACACCAGACGAATG +

AA>ABFFFFB4CBGEGGEEGGGHHCFCAEHHFFGGCHGGAEEHGGGEHGGGCEGEFCGGG/EAEHHEE//EF3GHCGHHGHHGFFFHFGFFHFFFHGHHH21FGFEEF1FGHG/F0FGCEGHGDGHHHHGAF=<GCCDC---.00000;0;009000000/;B09////...../////9.--9-//9////////////:///////9///////../.9///////////.9/.----.....--;9/ @M03555:476:000000000-CJGL9:1:1102:19032:3662 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCCCCGCGACAGGAAGGACACGCAAACAAGCAACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGACTCTCGTATGCCGTCTTCTGCTTGAAAACAATACCACCCCCCTCACCATCTCGTTGTTTTCACCTCACTCTTACTTTTTTCTCGTTCATTCTCCATGCTCATCCCCACGTGCTTACATGCACCCCCTCGCTCGCCTACTCTCTTTCTTGTC +

A?3>BB4CFFFFFFGFEE4EAEE?EAEG2FAA1F2FEGGCEHAEBH2FGGAEE/BAGHE/E>EHFB1??GHH/BG3F4FEHGFHEGG/EGHFFFHBBC/21BFEG<G<G1@E /?F1<1?GB1011?0.F11111<..----....00/00.;./:.//000009/.;000000=;0:-900..../0000///;9/////9/.9..-....////9/////9--;.--.9---.9.////9//////// @M03555:476:000000000-CJGL9:1:1102:14274:3724 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACACGTTTCAAGGCCATCAGATGTGACAAATCAAGGTGCAGTTATGAACTGGAGCCGTATCGATAGCGATGCACTGGACGGATGTTAATACTACGCAGAGTTGATGAGTGTTAGTTGGTTGACAAGGTGTGTGTAATACGATGTTATAG +

A??11BD111>BB1FE1EEA0A0EAHEECCGGHCHCBFGFCHBFFHHFH1EFGGFG//BFGHEGFGFG1DBCEECFFDEEEB?FHDBGGEC1BD0EECG////0/000122?1///0111111111111<1<1111<11111111111=00//.<<-.........0--;..0009//.--..-90000000009-.---9//9////////9/9/9/---9///--//;----/////--;-;///// @M03555:476:000000000-CJGL9:1:1102:21769:3779 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCACAGACCCCCCAGGCGGACACAGCAGCCCCAGGACCGCAGCCGACCTACCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATGGTCTCGAGTGTTGAAAGTAAAAGTGACATTACGTGTGAACAGTGCCTGTCTGCATGTGGGATTCCAGACATTCATTGTAAATTGTCATCTACAT +

AAA1>1CFF111BFFGEECCAGCFCCCEECEGCECEGCHCHHFHFHA//EFEHHECEEEHGG/EGHHGHC>EEE/EGFC@CCGHHHAF0GEHGHGGHFHFHHGFHGFECHHG <GG0FFGHGHFHGGBHFFG<<GFGGHHHHH;00CAC@ -;---.009;9/9/-:-//-//;/;//////////////-9-/-;/////////////////////9-//;////-9///9///////////9//////;/ @M03555:476:000000000-CJGL9:1:1102:11460:3943 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCGCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACGCTGCTTTTTCATCAATACATTGATTTGCTTATACTTTAGATCACATACGTACTCCAACGTCTGTGTTATATCTCTGTTTCGTGTGTACACTCCATCGTGCCGTATTATGTATCGTGTCAATCAGCTTGCGTTGCATTCTCGTC +

BBBBBF>DFFBFGFGGGGGGGCGEGCDDFGGFGCCGFFHGHGFHGFGHHHEGHHHHGEEEEHHFHHFGBFGBDGGBEEBED/EEFFFDGHHHG?BEECEGG/////////<111?10111111>111111<110<=0=000000=00000/0000;00..0/:;/.:./.00;/0000000000;00.....;00000/B/00....9-9..///9////.;.;.//////////.-..-.//////... @M03555:476:000000000-CJGL9:1:1102:20613:4021 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCCAGCACGGGGCACCCGGCGCGCATAGGAGACCAGAGGGACCCCCCCGACGACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATGAATGCACATAGTCAGTCAGGAGAGACATGTATTAGGAATGAACACGGAATTAGCGTAGAGTGGAACAGACAGACGACGACACTGTTGAATTTAG + BBBBBFFB4@FFEEEGCA2FCFBFE ?A2E2EEEEEGGE0ECGGFGB31?FFBGEF11FE>EEEGCEECCC//B<C/CFH@ <C?CGGHHGEHHFGHFGFHG0<GDG/DEHGHFGFGHGFECCCHFHGGGFHGGG-FEBFGGGGFFGGFAB?9--;.//////////////;/:///./.:....///////////////;/..--..////-..-./9;.///.//9../.--------/////9////// @M03555:476:000000000-CJGL9:1:1102:21555:4283 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCCGCTGCCTACCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATATCGTATGCCGTCTTCTGCTTGAAAAAAACTAGGAACTGCATCCAAAACTAACCCACTATATTACTCGAAATGGAGGCAGAATTCATAGTGTAGCGGTGTTATTTTGCGTTATTATGAAAAATACCATAGTCATAGGCGGCCTCCTTGCTTTGTACTGACACGTCGTAGC +

ABAAABF44CF?FGGGFGE?EEGDFHDC2EEC?EAFDA2E00AGGHA1FGCBFGBAGGHDD5FGFE11BAGHG@GHHEAG4 @BFHEB34@EFEFE?G4FF3F033?BE//1B10B1?1D11@G0FF1/0<1F10/?/?01111@ ?11..0.>1110.<<../<0=0000000=DB-:.-..0;00000-....90000;00.;;;FB0009;000;;9--;>..;:///9//9//;;/////.9.-;... @M03555:476:000000000-CJGL9:1:1102:14066:4687 1:N:0:CTGTACGTGA

GTTGAACACGACCAGAAAACTGGCCTAACGACGTTTGGTCAGTTCCATCAACATCATAGCCAGATGCCCAGAGATTAGAGCGCATGACAAGTAAAGGACGGTTGTCAGCGTCATAAGAGGTTTTACCTCCAAATGAAGAAATAACATCATGGTAACGCTGCATGAAGTAATCACGTTCTTGGTCAGTATGCAAATTAGCATAAGCAGCTTGCAGACCCATAATGTCAATAGATGTGGTAGAAGTCGTCAT +

1AAAAFF1CA@1EEGAA1CGB1BCGGGHFECEEEFHAFEFBGHHHHGHHHFHHFHFGHFFHHFHGFEHFCFEEGGHHFGFHGEEFCFHHHGHHHFGFFGGCGF /?FGFGEEGGFFDGGHHHGFGHHGHHHHGHHHHHHBCGFFHHHHHHHHHHHHHGGGGGGGHFHHHEFGFFHGHHHGHHHHHGHHHHHHGHHHHBHGHHGHFFHHHGGCGFGGGFGGGEFFFFGGBFBFFGF;0BBFGEFFFFFFFFF @M03555:476:000000000-CJGL9:1:1102:22433:5097 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCGAGAAGCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGACTCTCGTATGCCGTCTTCTGCTTGATAAAAACCATATTTTACTACCTTTTCACTTTTTTTAGATTATTCATAGTGTCTGCTCGAGTCTGTTGATTGTCATTTTTCCTGTTCGTTTATTTCGTCTACCTGCTTTTTTCTCTGTAATGCTTCTCCTTATATACTTACTATTCTTCGT + AB@A3CFBCBFFGGGFFAA2EE2GFAE ?EEEAEAFE20A?FABGEEAFG1GHHGFGHHFHHF@FHEF4FEF1BF11B3BFEFAGBFFCEEFGGGB4FEGH4F4E3B ///334422B1121B1BF12222>11///01111?@1?111??11==1=.->1<<1<<00=000000<00=/000000../..0=00/....0000./0000-/;000000;0000900;0000000/;////9;//;//.. @M03555:476:000000000-CJGL9:1:1102:15282:5266 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCGAGCAAGGCCCCGTAAAACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGATGTTAGAAATAGTTATTGTAAATAGTACATGTTTGGTCACTGAACGTAGGATATGTACGTGATTCTTCGGTGTAGCAGCGTGTTGCGATCACAGTTACCATGGTCTGATGCTGTCGTCTCTTTTTGG +

AAA3BCDFBF4AGEGGGGGGGGHHGHHGGGGCEDCHGGGGGFGGGCEA0EHGHFHHHFHHHGHHHHGFHHHHGFHGHHGFHHGHGEHDGHGFEHFHGG?EFGBGGGGHHEHHHH/@@<<-.<111111<1111<1<<000000<000000<00000/:.00/0000/.....00000000.//./00000...9.0;000/-...../--...////////////;//////9////9....////;-- @M03555:476:000000000-CJGL9:1:1102:16562:6082 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACTTTTTACGTTGGGTTGAACAAGTAATCGGGATTCGGAGAAAGTGTCGCAGATCATGGAACTAGTAATGTAGAACTTCCCTTGTATAGTTCTTTCGTTGATTGTATTTGCTATAGTTACTAGTGTATGTTGTCTCTCAGACAATGT +

AAA>AFFBC4CFBEEGFEEEEEGEEFEDGGEAE2ACHHC3FCHDEGED5GBGFHFHCFEEEGFGHGFFEE55DDFGCGFHE/?>GFGHFBGFH?@?3?@E/<<//112>1/1/00<0////011?0011111..-<.<0...--.//0000..-.:0/000000/000000000000000000/00/900000000000..:/.//////////:/9//////9//9:9//////;///9/////..//; @M03555:476:000000000-CJGL9:1:1102:22522:6191 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCAAGACAGGTGCGAACCCTGAGAGAGCCGAAGCAGCAGACCAAGCCTCTGCTACCGTACTGTACGTGAATCTCGTATCCCGTCTTTCGCCTGTATATCCAAACCCTCCCCTCTCATCCTCTCTCTCTGTCTCTCTTTTCTGCTCTTCTCTCTTTTCTCTGTTCTCCTCTGCTCTTTTCTCGCACCTCCCTCTCTCACGCTGCTTCCTCTTTCTCTTTTCTTTCCGTTCTTCGC +

A1>A@11BC1B1BA1F1EABA0FABFGHCC0A0A0A0B1CECGE ////E1AA0FCAGB/G//@0F11F110>F/>FD2GFFGCA/G1F1FE/00>>/?EG/22////?<>2>22B211/?<0//?/0/@0@ @F<<1<?11111111?<1<1111111111111FF<1>1>F00<000<000=/0000<=00;000..---/.;.//;99000..;..000/;09090;0000000009/:/9--;/-- @M03555:476:000000000-CJGL9:1:1102:7769:6296 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCGCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATAAGCTTGAAACTGTCTATAGAGCCACAGATGTGATAGTATCACATTCTTTTCCTCACCGCCATTACTCGTTCTAAGCTACTTGTTTATCATTTTCTGTGTATCTAATGAGCTAGCTTGAAACATTTGCCATTAACACAATACAGTT + BBABBFFFFFBF4FEBAEC?EEFE?FEGFFCEGGGHHGHFAGFHGHEHHHBFFHHH0FCECFDDHH2E1FB44@ ?BECCFDF?EHGBGGGHHE0B?30<E//>0222222202222111111@ <111000?0111<111111111<11111100000000...---.:0000:..;.000/000000;0/0900000000000000090009000090/00/.//////;///////////..///;//: @M03555:476:000000000-CJGL9:1:1102:16938:6471 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCGGAGGCAGGTGAGGAGAGCCGACCCGGGGGCAACCGAAGCCGCAAGGGGACGAGAGGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGAATAGTGGCTTTCCCAGTGTGTAGTGTCTGTTCTTCGTTCTTGATCTACTCCCCAGTGTGACCTTGTCATCTTTGTCTATTTCATTCCCCTTGGTCGG +

BBBBBFFBFFFFFGCGFFFEEAEEGFGAGBFHGGHGGCC00AEEGGGGG?FF?E/>EFGEE?E/E?EACGCC/B??FBCC??1FCCGHHC0CFFFGG=DHFFFHGCGGHFHHHHHHHAGFFHHFDGBGHC9@FFGFB0FFG.09C.A- ;--.../////..../;BF//:9//.:///99/;;///9/../.;///////////....//:////////////9//;//////;999////.:;.///-- @M03555:476:000000000-CJGL9:1:1102:16892:6779 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCCCGCCACGAGAAGGCCAGAGACAAGGGACGCGCGACGCCAGCCCACGCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAACACACCTCCCTGCTCCACTCCCTCCCCTCTTCCCCCCTTCTCCACCCGTACTCTCCTCCCCCTTTGTCCTTCCTCTTCGCCTCTCCCTCCTTCCCCACTTTCCT +

AAAAAFC4C4FFBFEGGCAFE2E?AE?EGGFCEEEGGC2BCFHHGEFGEEGEGGEEE1EF1?G/EEEEEE/<FDFHGG?@@BCGHGCHABGH1?1FDFHD1FC@F

AEFHHHGCC.<=0<=CE<G/GD@;:GECF0:G:0.:;CF@ .9..../.../0./00;:0//.........///.9.99..///;.;.-.-.://///9/..9-.//////////////.....///99...//;.9.;.////9 @M03555:476:000000000-CJGL9:1:1102:19994:7040 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCCAGCACTAGCCGCGGGCAACGGGACCCAGGCAAGCCGGCGAACGCCCACGCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAATAAGAATTTTTTCTCTTATTAAGAGTTACTTTATAAGTCAGTTATCTTACATTGCATTCGCCATGCGTCAGTCTGTTGATACTAATCACGCTGCCGCGATGTAC +

AAABAFFBCBFFGGGGFGG4BF4AFFGHG?E?EGE?F1E01B>EG1FCGEBEE1C/>E/EEGGGEGGGGGCGCCEGFH/</ACF0FGAGGHHHFFFG1DGFFHDCCCGDGBGBG.:CC.0CGGF.CFCG0;;-9C00B0CF0.0;00;00;0000;;-/C0/;B:B///9/9///9////99/9////9;/;///9;///////9/.;..9/.;;..;///9///9/////////...;9.-;--;:./9 @M03555:476:000000000-CJGL9:1:1102:12051:7410 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCACGGAGACAGGGATGCCACGAACAAGGGAGACATAGAGGCAGCCGCGCGACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATTCCGTCTTCTGCTTGTAAAAAATATATAAGTGTTACTTCTGCTTTCTCTTATAAATGTGTAGTTACCAGCCGTCACATCATAGGTCATCTTACCTTTCTTTGTCGTCGGTGTAGCTGTGTTAA +

AA1AAFFBBFFFFGFGGG1BEE0E?E0GGCAGFHHCHGEEE/ABA?/E/FHGGF1FHGGGGGGG/EEEE??C?/C</FE/EEGG0B00B<GGHFFDHHHHBHHFHCCHGGBD1??FF/FG1FGHGGH1>1F>GGG0GB0DDC<0=0=0-.00;0000;0;00:00:0000;00;0009;00090090000900;;9..-9-.-//://////////////9/;////;///-----;:-/9/;///9/// @M03555:476:000000000-CJGL9:1:1102:18295:7437 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCGTCCCGGCCGAAGACGGGAGATGAAGAGAATCACAGCGCAGTCCCAGCCAGACACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACACATCTTACTTAGAATGCTAGACATAAGAGTCGACATCAAATGTGTGTATTAATGTATTGGCAGATACGATAGGATGATGTAAGATGACAAAGGAA +

BBBBBFFFBFFFGGGGGEEFFFGGGGFHGGGGGGHHHHHHGFHHHHGGHHGGGGGHHHHHHGHHHGHHGEEDFEGHGGGFGGGHHHHGHHHHHHHHHHHHHGHHGGHGHGHFHHFFEGHEHGHGGHEGHGGGHGGHGHHHHGCGHHGGG-;-;....0000000000;00000900000000////-:-..//////;//..//9////////////..///...9.///////:/9////;//9./../ @M03555:476:000000000-CJGL9:1:1102:24541:7443 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCAAAGACGCGGGCAGCAACCGAGAGAGCCGAAGCAGCCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATAACCTTATATGGTTTCTGACTTAATGTCTTACCCCGACTAAGGTTTTTAGCGTTAGTCCATTATGTGTTAGCACCAAGGTCTTGGTTATGTCCTGTGTTTATGTTGTTAGAGTTTCTTTGTTTTTAT +

BBBBBFFFFFFFFFCGGGGBFG4CA2EGGGCEDFGHH?AEEE?EHGG?EECFGHHACFFHHHFHHHHHGHHFHHGFAEGFFGHHGHHGFHHHFFCGFFGG?CGEHFF2GHHFGGBCCC<---.000/=00000000:.000;000:000000000//.;----0//000.../0;9...//////////////////.....;//9///9///9/9//////.;9////..////;///:////....9/ @M03555:476:000000000-CJGL9:1:1102:19591:7469 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCGGAGGCGGGAAAACCAAGCCCCACCAGGCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAATAAAAATCTTCCTCTGCGCGAGATCTTAAAATGTTAGATGGTAAGTTTTCCATCCGCATGTTCCGCGCATCTACCAGTTTTGAATCTGTAGTAGTCGTCCTAGGCGCTACACACTCACCTTTA +

AA?AAFBDF4CF4FEFEEGE?EEEC?00E11G1F1BFEGGDF?HEFHGE?E/?EHH?/EEGH30?FFGF0?CF3FGFDHHHDG??/DCFGF2??G/?D11GHFFFHFBCCDFF<F1FGHH0G1FA--.00000:/000000000----9-.0000000000;00000/000000000;///:..-..9///.;---9-///////:9/..99/;///;///;/.....////9----/././///.//// @M03555:476:000000000-CJGL9:1:1102:27012:8059 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCGGGCCGCACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAACAAATCTCCCGTTTTAGTGCCCGATGGTCTAACTTTACTTTTCTGTTTCGTCTTCCAGCTGCTCTGCTATTATGTCATGTTCTCACGTCCCCCTTCACTTTCTTATTCCTTGTCCGTCTCTTTTTTTCCCAACTCATCCTTGAT +

AAABBF4DFFB?FBEAGEC??E2EAEEG?A101BE1AEA0EFGG1GCFAFA1FBGDFFDB4B2E?G1?BEEG1?GE1FGGFFC/FCFDEGGGEB44BFHHA22?CC///?222222//02/101<110//-..>111111111111000000000.....000:/0//000000000000000000000000.9.....-./0000000000000000///...../////--///;/./////////// @M03555:476:000000000-CJGL9:1:1102:8653:8172 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCCGGCGCCCAGGGAAATCGACGCCACGCCAGGCAGCAAGGCACCAGCAGGCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAATAAATCGTTGTATACATCTCGATGTTGAGGCTCCCATTGTCACGATCGGCCCCTTCAGCCGTTATCTTCTGTATGGCAACTACAAGCTCGTAGTGGAAATG + AAAAAFFBBFFFCF?GGGG0A0A0EAEGGGFFCGHCAECEE/BEEGCCFEHGGEHFGGGHGGHHHAECA?/E@EE <GGG?EGGFHFHEGHHEEH1FHGHHHHFHHGGGGHHHG1/<FAFG1=FFCAC01FCGG..DHEHHHH<CDGGC-.00;00:9./;000000;00.0:0.000//.9..9/000////-------;-9;--////-9--9//99//////--//9//////:99-;---/-/;/// @M03555:476:000000000-CJGL9:1:1102:8229:8802 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACGACGTTTGAAGTGCGGATTCTGTGTATGCGATAGGCAGTGGTTATGTGTATGAGTAGAGTTTAGGGCGTGAGAGTGATTTAACTTCTAGAGAGAACCGATGTATGTAGTGTCGCTATTTTGGAGGTGTGTACGATGTGAGAGTGT +

AAABBFF4CBFFBBEFGFGGCCEEC2EHFGCFGGGHFHHHHHGHHHHHFGHHHFHHGGGGHGHBHHCGFEHEFHGGEGFFBEE@ ?FEHHEHF3BB33B/EE/<///////<0<111@ ?1/-<-.11111>1000.....//0.;//0.00;00<00000;0000;00000./---..0/;0000000000000000/..//.9--../;////;///.....;///-//.../.9//....9/////9/: @M03555:476:000000000-CJGL9:1:1102:6564:9238 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCCAACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAATAACCTCTTATAGGTCTTAATATTGTAAAATGTTACTCCGTAATATTCTTCTGTGTTTCCAACACTGTAGTTCATACATCATACTGTATGCTTAATTGTACTGTCGTATTTGTTGTAGCAGTAACTATTCGTACTTTCTACTTATGT +

ABAAAFFFFBCFGGCGGGAG42E2EECFGHHE22AECGFGFBGEFAAADFFFFHHGFHCGHGGHFFHEGEG155DFGGGHHGHGEEFFHHGHGHEBB3FH///03333301112221B1112?B22222222221@22

2@1 //0//11111111111<>011111..1111=1<=0<000==000000<0000:00000000000;:...//00;/./0;0000;9000;00///9.009990000000 @M03555:476:000000000-CJGL9:1:1102:7383:9326 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCGGACGACCGCTCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGAACTTGCGCAGGTGAAATGATCAATAAGCAGGACATATCTTGATGCGCAGACTAAACAGTCATGTCGCATCGTGAGGGTATTGCGTGGAGATGCAATCGCTAGAGTGGGGTTACGTGCGGAGGTGAGACTAATA +

BAABBFFFFFBFFGGFFCC2?EEEAEAEFGGGGCGEAHHGF?>EGHFGFGGGH1FBEGBHBGFHF?>FEACGFHHHFBA/FDEGFFGBFFHGGCGEFFFGHHF0FG?FC/@<>--.../0000-----./00000;00000;0:00/.../0000000;090-.---./000/0./////;/.9-.-.../...9/////9..-..////////;.9..//9/..-9-./.;..-;---../.9////// @M03555:476:000000000-CJGL9:1:1102:20651:9542 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCAGCACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACCCTCTTTCCTTTCCTTTACTCCTTTTTTCCTTTCTTCCTATTTTTTTTTCTTTTCTTACTCTTTGCTATTTTCCCGTTCTCTCTCTTCTCCCTCTTCCCTCTTACTCTTTCCTATTCCTCCTTCTCTCCTTCCTCTTCTTTTC +

AAAAAFB1C11B1BEGCECCFG00AE0AFEHFE/EEAC0EGFB0AB/01DF1FFHHBGFAFHGHBEGHCF0B>DB2B0F/B0@E ///BF2FBH1B10BGD0>///////0?011211@2 @1122111?111111/0>=111111111<1111---;::0000:000:0<00000000;00B0.:./90009000;000/9//009/99-9////////;/////99//9//9/9//;//:/://///;// @M03555:476:000000000-CJGL9:1:1102:8705:9687 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCACCAACGCAAGCAACAACGAAGGACGGGATCGACCCATAAACCGCCGGCCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAACAAACATTATCTCTCCTTGATACTTCTACCCATTACCCAATTATCGACTCTCGTCTCCACCTCCTGTCTCTTCTCTTACGTTTATCTCACCTCTCTCTCACTTCCC +

AAAABF4B444CCGEFGFFFGFGD??EEEGFCCEG2EAF?EGG0ECFGGCDC0E1FHFGHEEEEGFCEFGGGC/?A?///>@G2CFCGGHBC02?GFGG1<GFGGF/?G1FF1C0<.>1FGHF?G/DDCAAGEHHHGB0:.:0</...000000000/;000000;000000.0/0000/0.900;0..-.;/:...../9......////////999//;..//./////;././/9/////////. @M03555:476:000000000-CJGL9:1:1102:12244:9872 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCCGCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATTTTGAATGTGTTTACAGAAGACAACATGTAGACTCCGGATGCGTTCCCATAATAGGGAGATCAGCGTCTTGATCTCAAGTGGCGACTAATTCATGGGTAAGCACCTTGCCTGGCAGGTGGCTGATAGTTGCTGACGCAGGAGTG +

AA3AACCFFFBFCGGGGGFEEEEF?EFGHHG2AEGHGHHHHHGHGGF5FGHHFH5GFEFFGFDDGHGHHGFB4BGFFACGFDE?EHGFHHEHHHAGBBGFGG/</0<221?1111110011111111000.<01<111110/.<..-<-.:./00;/000:0.:../0000.....000000000000.9----/99/9///.//////9.///////9...-99.....999///////9..---.... @M03555:476:000000000-CJGL9:1:1102:13770:10094 1:N:0:CTGTACGTGA

TAAAGCTGGTAAAGGACTTCTTGAAGGTACGTTGCAGGCTGGCACTTCTGCCGTTTCTGATAAGTTGCTTGATTTGGTTGGACTTGGTGGCAAGTCTGCCGCTGATAAAGGAAAGGATACTCGTGATTATCTTGCTGCTGCATTTCCTGAGCTTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTCCTCTGCTGGTATGGTTGACGCCGGATTTGAGAATCAAAAAGAGCTTACTAAAATGCAACTGGAC +

BBBBBFDFFFFBGGGGGGGGGGGFGHHHHHGHHEFFFFGHHGGHHHHHHHGHGFGHHHFHHGHHFFHFGHHHHHHHHHHGGFFHEFHHGGHHGGHHHHHHGFGCEHHHHHHFGHHHEHFFHHFGHHHHHHHHHHHHHHHHGHHHHHHHGHHFHHHHHHHHHHGHGGGGGGGGGHHHHHHHHHHHHFHHHHHHHHHGHHHHHHGGGGGGGGFEDGGCEEFGFGGGGGGEFGGGGGGGFFFGGGEFFFFFF? @M03555:476:000000000-CJGL9:1:1102:24319:10186 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCGCAGCACCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACTTCCAATTTATACCCCATCTCCGCCGATCTCCATCTCTTTTTTGTTCTCCATGCTTTATGCTTTTTACCCTATCACACTCTGGACCTACCACTGCCTATTACCTTCTCTTCAGTTTCCGCTGTGTTCGTGATCTTCTC + BBAAAFFFCCFFFGEGGGEEEEFHHHFGGGEE2AG2EEEAEE3BGFFAAEAGFFHBD@FFFHE @EGDGDD@FFGF1F4 @FGEEEGFHFFGGHEFHHHHHHA??3BE/>B///111111111?11?11..00111...----.000000000000--/:0000000/900000000000.00/0/90000///9000...//.///9//9//9//////////////////...-.////.../9////// @M03555:476:000000000-CJGL9:1:1102:4919:10268 1:N:0:TTGTACGTGA

AGACACAGGCTTCCATCCGTATAATAGCTTGCCCAGACCAAGTCTCTGCTACCGTATTGTACGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACTTTCTTCCCGTTTTTTTCTCATTTGCCTCTCGTCCTCACACTTCTGCTATTCTATATTTTTCGTTTTGTTTTCTCGTAGCCCTTCGCCTCTTTTCGCCGTCCTCCTCCGTTGTTCTTCCTCTTTTTCGCCCCTTCCCTCCTCACCATTGC +

ABABAFCC>AFFGGGGGGGGGGHHHHHHHHGGHGHHDGHGFHHHHHHGHHHHHGGHHHHDFHHHHHHHHFGGGHGGGGGGCFFHFHGHH1FEFHGE//>/344B444?2//03/?//2@

22222222222/?///11/110<<11111111111=1=<110-</.../</<000.....0/;/:...../0000;9.---..0//9/..0...//:///////99/-...-..././...;/..///// @M03555:476:000000000-CJGL9:1:1102:23313:10618 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCCCCCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAATAAATCTACATTTTCCCCTCTCTCTCCTACTCCAGAACTCCTCCTCTTCCTCTCTTTTCCCTGCCTATTACTTTTTTTCCTCTCTTTTCTGTCTTCCTTCCTCCCATTTTTTTCTTTCGCTTCCTTTCTCTGTGTTCCTTGTTTGCTCT +

AA3>AFFFFFFFBFAFFGEF?EAEGG2221BBE0E0AB3FFC3BGGAHGGHHHGHFHHHEFEEEBGGFGFEC1@4F4EFE3 ?4B/?/FFFH44??303B??0233333333333332222/222222222211?1?1001111?00>>11111101111>11<0..<0=00000000--/00<0/0000000000009000/90/.900000-9/;///...../9///9/9///;////////.///// @M03555:476:000000000-CJGL9:1:1102:26419:10696 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCGAGCGCCACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGGTTAATGAAGTGTGTAAAGGCGTAACTAGAGAGAGTCAAGCTAGAACAAGAGAATCAACGATACATCACGTAGATTGGCAGTAGGCGTGGGGGCAATAATGTAGCGTGGATAGAAGAATACAGATTGTAAGTTGGT +

BBBBAFFFFF4FBGAFGEECFGGGG2BEEFGFCFDGEEAAECAEGFH1FFCFFGHHHFFHGFHGFEEGHFGHGGFEAG4FHHGFGHGHCFGGE4GBDGFGFB33FC@ /?<</-..111111111<100.00<00.-.-..0<0000.../00:00/00000////.//0;009....-00000..9..///.//./////9.-------./////////-..-./9///9////9/////////9////. @M03555:476:000000000-CJGL9:1:1102:18990:11861 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATATCGTATGCCGTCTTCTGCTTGTAAAAAAAAAAAAGGTATGCTGCTGAATGACACTGCTACAAGCTTGCATCGAATGTCTCATGGCTGCAGCGTGTACTATGCGTTAACTCGCAGAGCCTATATGAAGTGTTTTTTCTCATTTTCTTATATGCTGATTGCTGTCTGTGTGTAATGTTAAC +

AB??AFBDF4F?4AEFGEF?2EAEAFDFFC?E?C32GFFF1CEGGFFHGFFFFFAEAAAGFDGDG2211DB@FFHA ?FFD?AEHFBDGGHHH144443E?>//////22222222<1<111?1<1?111<1111.>10.11<.001/00000000./<.<//-..:/000000...9.000.--.-././:00000000000//.-/9/;////////////////////;/;///;//..///////// @M03555:476:000000000-CJGL9:1:1102:26167:12157 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCAGGGGCTCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGATTATAGTAATTAGAAAATGTAAATAGCATAATCAGAGTAAAACTTTACGATGGTAGACATAACTGGTGAGGAGATCAGAGAATAATGGATAAGATGAAGTATAAGAGACATGGAAGCAAACGAGGTGGAGGTGAGGA +

AAAAAFCDFFFFEGGGGGGGGGGGGDFEEGFGGGHHCCECEFGGGHFCHFGFBFFEG5GHGHFEFFDHDBDFFFFGGHBGHHGGGFHFCEGHGHHGGHHHGFFB0ECC>////111111111111111101111111111111<11111000000000000.....000000000/00/0/0.:0../00000//0000000000000;0;;000;000://./9./9/.///.:..-9......./.;/ @M03555:476:000000000-CJGL9:1:1102:26032:12278 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGTCTGGATCGGGTATATGAGCCGGGGAGGCCTGCACGACATATGTCGTGGGTTGATCGTAAGCTATAGTGGAAGTGCGTGGAGCGTTGATACCCCCCGTTGTGGTTGTTCTATGTATCCACTTGTGCTGCGTACGTTGCGTTCGA + BBB@AFBFFFF4B4ECCEECEE ?EFCEHGGFCFGFHGFHHHFFGH5FGDGHGFFF2AFEEHGGFHHFGAFHBE4?EEHHFGEGEHFHHEBDH31?E30EEE/////22222?21//<//11<11<10<------../..//..--.0:000....9..-/00.9...00000000.//////-..-.:.-..-./////.---9.;........///9/////////////////-..--./9.-..... @M03555:476:000000000-CJGL9:1:1102:6998:12350 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCGGCACAGGGAGGACCCACGCGGAAAAAGGACCAGCGCAGACGCGCCAACCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATTTTTGTTTTCCTTTGTGATTACTCATTACCGTTTGCCTCCCGGTCCGTACTGCTCCGTTCTTCTGTTCTTCTCTGCTGTTCACCCTTTTGTACTTCTCT +

AAAABFFFFFFFGGGGGGCCE?EC2GGD?EDHCEGEEFFGCC0FG?FEFHHHHGCEGFFFGGCEGGGGE/E?>CDHHFCC/B<FFHHHHHHG<FFHHFHHHHHHGF..FFGFFH0<CCFFD0CCFC/BDCDCHGFF0GHFB:0:G:-9--./00099;////9/9/9.////////9////;9././///...-----....///9/.;/..////////9/////////////:..////.///////; @M03555:476:000000000-CJGL9:1:1102:22686:12408 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCTCAGGGGCGGAAAGACGCGCAGGAGCAGCAGGTCGAAAAAGAGAGGACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACAGCCGCTTTGCTTGGGTACGCCAACAACGACTTATTACATATCGGAATATTGTAAACTGATGTCTTTAGGCTTGAGTCATTACGGACAGCGCGATGTGCTT + BB@AAFFFFFFFBBEGGGGG4GF4ECE ?EE?FBGGCEGGGGAFGFFHAHGFC>/11EAEFGGDDHFC/EDDC/GF?@/DGBGFFGCG/?CFFDD1GFGHFFHCFGCHFGFGE0<.CG0DGHE</CFF@ ::GGCGHHGFG.BC0:B-9@ ../..--9../////..;//..-9-....-.-../9////////.-..9//////////9///9//////////-./99/////...--..-9---../9/: @M03555:476:000000000-CJGL9:1:1102:16595:12416 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCGGCGGCACAACTGAACCGGACCAGCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACTCCTTCTTTCCTTTCTTCTTTCCTCCTTTTTTTTCCTTCCTCTATGCTCTTTTCCCTTCCTCCTTCTTCCTCCTCCTCCCTCTTTCTCCTTCCTCCTCCTTTCCCCCTCCTTCCTCCTCTTGCC +

AA11ACC1F1BBFG1BFE1AAEE/E/00AA01B1FAE/AE>>>E>E/E/ECGG/>>/C0F0FC0F0/>BFGHF111FHB?//??DB>GHHH/<2FGG0</?F2FC<<0/11FFFD1011><>---.00:0000000;;0;00;<00000009/;000---/9900/00000/////:;//////-/;//-//9//////--/;-/--9B//:9//9//99//--/-/////----9-//////--////9 @M03555:476:000000000-CJGL9:1:1102:24247:12487 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCCGAGTGCAAGCACAACCCAGCAAGGGGCCGACCCCACAACTACCGCGACACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAACACAAAACTTTTTGCCAGCTCCTATGGTCTTGTCCCCATTTCTCCCTATTTCCTCCTCTACGTATTACCTCTCATATCTATTCACTATGTTTCTTCTTCTTTTT +

ABABAC4DF44B4BEFG?CFEEFHFH5AGFHHFEEECEFF2GBB20AEEGGCG?EEEDCGCFCFGGGGEE?EEGEGGHGG/@EFHGHFH0GF/B2FGHHGFFDFHF/FDGHDH1<0FFC1GHHH..001F-.<C.0DDFFBCC0=0/<......:0000-0000;0///0000000000000.//0;0000//00090000/..//9..//9////////////;//////9///;////////////// @M03555:476:000000000-CJGL9:1:1102:22310:12552 1:N:0:CTGTACGTGA

TACAATGTGCTCCCCCAACTTGATATTAATAACACTATAGACCACCGCCCCGAAGGGGACGAAAAATGGTTTTTAGAGAACGAGAAGACGGTTACGCAGTTTTGCCGCAAGCTGGCTGCTGAACGCCCTCTTAAGGATATTCGCGATGAGTATAATTACCCCAAAAAGAAAGGTATTAAGGATGAGTGTTCAAGATTGCTGGAGGCCTCCACTATGAAATCGCGTAGAGGCTTTGCTATTCAGCGTTTGT +

BAABAFFFFFFFFFGFEGEGGGCHFGHGHHHHGHHHFHFHHHFHHGGCFGFGAEFGGGFEGEEEEFGHGFHHFGEGHGHHHG?EFEHHHGGGGGHGGGGGHHHGFFCGFGFHHHHGHFFHHHHHHGGGGHHHHHHHHHHHHHGGGCDGHHHFGHGGHFFHHHGGGCHGGGGGFBFFGFGGGCFGGGGGGFFFFGGGGGFFFFFGGFFFFFFBFFFFFFFFFFFFFFAFFFFFFFEFFFFFFFFFFFDFD. @M03555:476:000000000-CJGL9:1:1102:22685:12632 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCCAAAGGCATCGCCGGGAGAGCGGCCACGGACGCGAAGAGCAAGACCCGTCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCCTCTGCTTGAATAATAAAATACCGCATCCTTTCTCTTTTCTATATTCCCTTCTTCTCTCTTCTAACTCCTCCCCATTTCTAGTTCCTCTTTCTTGCTCCTTCCTTCCCCTGCTACCC + AA@AAFF11 @>FBGGCGGG1B0FCCCGB0EFCEG/EC//E/A/AFCC/E//>/>>/0F10FHB0?/?/?E/E///?/G//@//<F00<GFACGAFD1F1?<11?0><>CG1FD>.</.<0=00.<<<GG0:C-..00::0CH.:0090;0900090;--9?/;90;CB;0B00;900;;00/;BB/////;/;////////9/-;---BB/////:9//:/9:/BFF//;9//FFBFF//-9;-;://-- @M03555:476:000000000-CJGL9:1:1102:13969:13241 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCGCTCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAATAACACCCTATTACCATCCACCCGGAACTACTCAATAATTCACACTAGCTGTAACTATCACCTATCTGGAAGTTGTTGACTGTGTCTTGCTCATAAAGCCTATCCTAGGTACCGCTCGACTCCTGCAAGTGTTATAGACTGCGCTTGT +

A3A3AFFFFFFFGGEGGGGCFFGG2EEFAFHHGGGGG33GCEBGHHGHHGHGHHHGFGHCFEEEFFFHGHHFGHBDFHGEF3ED@EEHG4GHHHHHGEGFF /03333B//?22B33?3320220/////>1111<11111111<<11<01<111111=11000/</000000//00=0..0:0:00:00000000000000.//00/900/0090.-;----9.////9//;////;///////.-99.. @M03555:476:000000000-CJGL9:1:1102:23933:13470 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCAGGGACACGTACCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGAAACTTTAGTCCTTACAGTGAATATACTTCTCCTCTTCTTACTTTCTGTCCACGTCTATCCCACCCCGTTACATGCGCCTTCGTCCACTGCTTTACACCTAATCGATTCTTTAGTACCTCGTCTCCAGTTCATC +

BBBBBFFFFFFF4BEGGCEGGG2FFHDHF2FEEE?EEA2DHE0AEGBCFGFCAFCFGDGHHHHHBDGHEFEEFFHHHH1>>?E/FDGFDHH4FE/<FFGGBEFGFABFGFCC?///0000111111111111111111111>0000D000/0000=000000;0000:0.:./:00//0...--....0;00....=0./.9.//9//9///;/////9;..9/;/://///9///.9...///:///// @M03555:476:000000000-CJGL9:1:1102:17196:13550 1:N:0:CTGTACGGGA

CATTCAAACGGCCTGTCTCATCATGGAAGGCGCTGAATTTACGGAAAACATTATTAATGGCGTCGAGCGTCCGGTTAAAGCCGCTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAACACTGACGTTCTTACTGACGCAGAAGAAAACGTGCGTCAAAAATTACGTGCAGAAGGAGTGATGTAATGTCTAAAGGTAAAAAACGTTCTGGCGCTCGCCCTGGTCGTCCGCAGCCGTTGCGAGGTA +

CBCCCFFFFBBBGGGGGGGGGGHHHHGHHGHGGGEGCHHHHHGGGGGHHGHHHHHHHHFGHGGGGGGGGGGGGGGGGHHHHHGGGGEHHHHHHHGGGGGGGHHHHHHGGGGHHHGGGGGGGHHHHHHHGHGGGHGHHHHHFHGGGGGHHHHHHGGHGGGGGGGGGEGGGGGGGGGGGGGGAEFFFFFFFFFFFFHFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFEFFFFFFFFFFFFFFDFFF=DFD< @M03555:476:000000000-CJGL9:1:1102:9491:13578 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCACGCAGCACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATCTTCTTTCTTCGTCGCTGCGAGATATGTACACGATCGATCTCTGTGGTACGTTTTGTCCGCTAGTACTCCAGATGCGTGGGTCTCTCTGTTACATCCGGTTCGCCATCCGTCTTGTCTACGAGCAGTGTTGCCTGTCGT +

BBBABFCDFFFFGGCGGCCGCG?EEC2EEFEEDEGFGE?EEABFHFHHFHHFGHDGHHFHHHHGFEGHGEDHGGHAFFFGFGGEHHFDFEEHEDFBGHHHF3B3?/<///02>2222>2221/0/////.----.<=0<0000.-..-....0;000/:00.///../00.-.-.90090/;/0;00-..--..99/////9/////9.--9.--.../.9....//;///...--///9/.//99//.. @M03555:476:000000000-CJGL9:1:1102:18849:13691 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCGCAGGCCGCGGCCAACGCCGACACAGGAGGGAAGGGACAAGACGATACGACGAGAGAGCCGAAGCAGCATGGCTCTGGTGGCTCTGGCTTCCGTCCGCACGGGAGCGCACACCGGAGACCCGGATACGAAGGGCAGTGGCCACCGCGCGAGAGAGCCGCAGCAGCAGACCAAGTCTCTGCTACCGCACTGGACGTGAATTTCGTATGCTGTCTTCTGCTTTTAAAACAAAAC +

A11AAFFB11B11FE1AE1E0A0F000/A/E///A//A/>//00/>F///BE?//?F000>>>FEE/??<>CC?///?//>/CB000F1AFBF0?1<0.CGHFBFGF0FGA0=.-<<<@C?CC?9-;--;.-A9--9..@ ;-A-BE--9ABEF-/;BEFF--A=;=@---;--;-;-;9@ -:9A-/A9--;9//;////9/9-----/-/--:---//9/;;BBFF/B;//;/9/9;////////---;- @M03555:476:000000000-CJGL9:1:1102:4037:13710 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCTAGGTCACAGCATGAGACCAGACGAACCAGGCAAGGAACAGAACAGGACCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGTTTCGTTTCCTTTCCCTGTTCTTTTTTACCTTCGTAGCTATTTCTCTACCCACCCTATTGTACCGCTCTTACCTCGTCCTGCTATGCCGCTCTGTCTC +

AAAAAFC11C1CBFEG1EG3AFGBFFFHEC1FGFHF00F00AAAEEAEGHGCFFFECGBGFHH0FGEG>>EEEA//F@EE ?EHHHHFBG/F/>BEHHFHHHEFGE</?CFBFFAFF//2FHHFFAGEHHGCGHE1?GGDFF01110--<-<.0::0.:;:/00000000000;000009.;090/:/9..000;00000009-/:---9///////-----//:///-----//;/9///--;--/9/// @M03555:476:000000000-CJGL9:1:1102:26477:13729 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCCAAGCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATCGTTTTTCATTCGTTCACTCCTTACCCTCTCTGTTCTCATATCTGCTGTTATCTTGTCCACTATCTCTTTTCCTCGGTGTACGGTTTCTTTGCCACTGCGTGTCTCCTTCTGTATTCGCTTGTCATGCTCTGACTTCTTGCC +

ABBBAF4BC4CFBGEGGEE4F4AEEE?EEGGGG?EAE33FA1ABGHAG5GHFFDGHBDF?AGABGF5F?FFE15DGHHF?CGFFEECHF4F4F4BGFCFHGE/</</01/?//??02@111 ???1111<1111001.0011<=D111=110000<0=00=0000000=00/:0:;00000000;.:..00.99.:900000900/00.:../00000////////9;.;.9/////9//////9//9/// @M03555:476:000000000-CJGL9:1:1102:16732:14123 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCGAACGACAGCAAGAAGACACCGGCCAAGGACCACGCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAATCTCCTGTTCACTCTTCACAACTCCCCTCATTCTGCTCTCGTCCTCTTCACTGTCTACATGCCCCACTCCCTTTGTCCTTACTAGGTCGTTCGCCTTCGTTCGCTATCTTACA + BBABBFFFFBFFGGGFFE?2AEGEG?EGHHHHHHBGGHDGGE0AGGEFHHHGA1@EE //??AGGGCEEHFFGFFGABGFB?DGHFBFGFGCEGFEDFGGEC?CCG22?FFGGGFG/?<CFGFHHF1<011=GFGC-C./0000/:0000000000009./90..;;.0000000000.....90000/////9///9////..../...////////////////--...-.9./../.9-.9:////;/ @M03555:476:000000000-CJGL9:1:1102:21310:14380 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCATCCGAACACAACAAAACACCGCGCACCACAACGCAACGGCCACCATGGGCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAACAAATGTCTTCTCCCATAATTTTACTGTTATTCCCATCGTTCGTCACTGGCTCCACTACCATTGACTTCTCTTAGCACTTCGTCACCATACTCCACCCCCCT +

AA?A?FCDCFFFFFFCBEEDFGG?AEAEGCEH2AEGFEEGCEE0EEGG1FEE?@EE?CEGCEHFGHHGFE/E/>CEEGG/<E/BFGAFFGHE?F1FGFHFHGEGHCF/AGH?1<FCHHGHDGHC<FC?CAC<GFFDDC<<GF.A.;..0000000000/;9/0;000000000000000099.........//.../.9.;//.:////////9///////////.9...////.////...---- @M03555:476:000000000-CJGL9:1:1102:25107:14445 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCTCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGTTCTTTCTCTCAATTACGTCCTGTTTTTTCCGATCTTTTCTGTGTACTACCTGCCACCACTCACTATACTCTTTTTCCATCTTGTCTGTACTCTCTCCTTACTACCTATCTTGTTCCTTTTTCGATCTGTCATATTCTATCTTCAC +

A3AAAF>@C4CCBG2ABA2AEAEAE2BFGGGGCEFGBEGGFFB1F5FFFFFGHHHHGGGGCEG5GGGHHEHFFHFG1?BGG/E@HGFGF4FGF1 @3@1EE//03B2B211222211B?211/<0//111@ @/<01/<?-0111<>1111<1111010../0<../<//00<0000;0<00:0000000;000;00;;09909000/000/0000000000000;00/-.;../////////////////9 @M03555:476:000000000-CJGL9:1:1102:26747:14751 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCACACCACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAACATACGTACCTTTGTTCCGCATCGAAACCTTGTAGTGTTATTGATTTGGCTCGCCCTTGCGATAATGCCGTGTTCCCAGTCCTGACTGGCTGCTCCCCCCCCTGCGTTCATTCTCTATATCTCGAGCCGCAACGTGCTGCTCGT +

AAAAAF11BCFFFFEGGAAAB00BFC?E0ACEEGE/EEGFHFCGHFA0AEFFDGHF1EFFHE//EGF2FGEGGAF21BBGCBEFG1>?EFF2B1BGFCAG11B/////B11011//2B11>012//<///0<//00111<11011<1<111111111<--<.-///--...0000-;-:900000/00000/90..9..000...;--;------:9/////////9//-/---9----9----;/9/-9 @M03555:476:000000000-CJGL9:1:1102:19458:14867 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCCCGCCGGAACCCCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACACTCATCCCTCAACTTCAAATCGTAGTACACTATTTTGTGTGTTCAGCATACTGCAGTTACATGGATTTTCACTATATGTAAGTCGTCTAGAGGAGTGCGCGGACACCGATGCCTACACGTTGAGCTTGCTTGG +

A1AAAFFF1CFFGG1BFEGGGCEGGC/EFGGEGGGGGFAHFE>>EEGFGCFFGHHHHHHHHHEGHHHFEEGGHHGBGGHGHEGE2BGFCGHHHECCHGHFFFGDG0BFF0////<<.0100111.0/.0/00000000.....00;00:00000;//..90000000;0000/0;00000/000090000;0000090000-9---///-----/------9-9-9---///-/------/////-///- @M03555:476:000000000-CJGL9:1:1102:7200:14971 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCGCCGAGAAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATAATGGGAGCAACGAATGTAAGTCCTACCGAACGGGCGCCTCTCCTCCATCGTTTGTACTCTTGGTCCCGTTTCTGTGCTTCAGCCTCCAACGTCGCACCCTACGTGCGTTCGTTTCTGCCTAAATAAGACGCAGTATATATTTCAAA + AA?ABFBFFFFFGBEBGGGGEEA222EGFGCCGEFAA113BA211GFHEF5FHHGGEFFECBD@EFBF1BE44FC ?1F3GBEACAFGB4BG?30??3BEE/>01B22100//?00////111111111110/.---..---;..///0///00.;.../00900000090.-..//00000;00000///..9/...-----.;......-..9.././//////////9/9/.---.////////9/// @M03555:476:000000000-CJGL9:1:1102:25520:15029 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCGCCCACACGTAGACGGGCCCCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATGTTTCGTATCACTACACTAAATTCTGTAGATGACTTGGAGTGTACGGATTCGTGTGGAGGCGACGATATCCATGTAAGTATGTCTGCTCTTGTAGCTGAAGATCTTGAGTTAGTCAGTCATCGT +

AAABBFFF4FFF4GCGGGGGCEEFGGGHHHCGFG?FEEEGGEF0EFEHGE?CFGHEHFHEGHFHFHHHGHGGDGHHGGGGHHHFHHBCGCGBHGA?/B?GHGD@FFBG2BDGH0 @GFHGCFG<--<00000..//.0000//:0/:<000;0000;0090;9000//.000.....9./....9...-----..-//////////////;/////////9/9////////;/////////////99/9/. @M03555:476:000000000-CJGL9:1:1102:7501:15264 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCCGACGGGCAACCGACGACCAGCAACAAGGGACGGGCAGGAAAGGCAGCCTCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGTTTTAGTATGCTTGCATGTGTCAGTATAATGTGTATGTATTGCATATCGTCTCAGTGTGGCGGTATGGGTACTGGTCTAGATGTGTGTAGTCGTGGG +

BBBBBFFFFFFFGGGGGGGGGGGGGG?EGGGGGGGGEEFFHHHHFGFCEFGGCGGGGFHHHHHHGGFHHGAEF@EEA ?C@D @DFGHGHFFGFEHHHHGHHHHGHHGFADHHGGGE>FEFG1>FECGGHGFD.<GGFGHEHGH.:C0:C?-9-.00000/90000900.///;//////////////;//////9/;://9/.....//:///.9---..;..://://////////;/9../;//.;..- @M03555:476:000000000-CJGL9:1:1102:27893:15299 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCACGGCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATTAAAGAACCGGCTTCATGTTTATTAATTCGCGCCTGCAATCTCCATCTATTTATCCGTGTTAATACCATCCAGCCGCACCATGTTGAGATTTATCTCTGTCCTTTATCCATTCGCCATGTTTCTCTTCTAACGAGCGTCATAT +

A3AAFCBBFB4BFEFGCA4EE?EE2222C2F2A0A0A3AG1EGFAEG1BBEFDGFDFEBGFFEGFFB41B2?FE4BE??>FFF3>?EG/FFGFDFC033?0//>>0111?1100//////01111111>111111..---.../00000000:0<0000000.....00000000;.00/.---9-.000000/.//////;/////////9////;///...9////;/;///;///...--....// @M03555:476:000000000-CJGL9:1:1102:20766:15353 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATAATTCCTTCTATCTTTTTCCTCCCCGCTTTACTTCTTTCTTATCTTCTCTCTCGTCCTTCGATCTCTGTGCATCTATTCCTTCTGCACGCTTCGCCATCAGTCTTCCGTGGCCTTGTTGTGAGCTTGTGCCTCCTTCGCATTCCTCGC +

A>1A@ 1C1CFGGEGGE1E0A0EAECG?EEE?FFG0A0F/EAGBFFA21AG1FHGEEEEHFFFHCGAFF1FHHF?FEBGEAEEE2FBFGHFABD1B/@E<01122212BBB222122>101110/////</111111<11111<=<11111111..<..00..=.<0000000000000000;00000/.-..99..../09000000.09--.9//9/-;-////9/9///9/-;//--;--////-- @M03555:476:000000000-CJGL9:1:1102:11894:15381 1:N:0:CTGTACGTGA

TGGTAAAGGACTTCTTGAAGGTACGTTGCAGGCTGGCACTTCTGCCGTTTCTGATAAGTTGCTTGATTTGGTTGGACTTGGTGGCAAGTCTGCCGCTGATAAAGGAAAGGATACTCGTGATTATCTTGCTGCTGCATTTCCTGAGCTTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTCCTCTGCTGGTATGGTTGACGCCGGATTTGAGAATCAAAAAGAGCTTACTAAAATGCAACTGGACAATCAG +

ABBAAFFFFFFFGGGGGFGGGGHHGHHHGGHHGHHGGHHHHHHHHHGGGHHHHGGFGHHHHHHHHFHHHHHHHGGHHHHHHHGGHHHGHHHHHHGGGGGHFHHHHHHHHHHHHHHHGHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHGCGGGGGGGGGHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHGHHGGGGG:EGHGCGEGFFFFGGGGGGGGGGGFGGGGGGGGGGGGGFFFFFFF0 @M03555:476:000000000-CJGL9:1:1102:5904:15557 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCCCAGCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATCCTTTTCATTCCCTAGTTCCTAATATAATCTCAACATGTCTAATGTCGTTATCTTTCAAGTTAGTCCGTCGTCTTCACATCATTATTGACACACACAGTGGACCCATCACAGTTCCTTCACTACTACTTGCTCGTGTCGTC +

A>>AACCDCFFFGGGGGGGGFEFGG?F?EEEGHGGEFGHHHFFHHHBCGGHH5FDFHHHHGGFDGFHDGHHBGAF4FFGGGHF4GGEGHGDGGFHGFFFGH0<<//>/02222><21?1111<<01111111?1>1<1<11110<01>111110<0.<<..000000000000000.:.--...00000009;0;09;000/...;.00/0//..////://///////////;/////9//.9...... @M03555:476:000000000-CJGL9:1:1102:24524:15829 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCGCGGAAGACACAACGGAGCAGAACCACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAGATGACGATCACAGTATAGATAAAAGCTCAGAATGTAGGAGTAGAGATGACGAAACCTCAAAACTGATGTACAACTAGGATCGTAGGATGGGTTGCAGGGTATGGTAAGAATTGAATG +

BBBBBBFDCFFFGGFGGGGGGGFGFF33GCCEGGFGCGHFCGAGGGEEDFF?1FEEFEEFFFFFHHHGHHHHGHHEFHFFHCCGGHHHHHEFFAF44BFFFFHGHFGACHGHHHGGHHFFHFHG??<-----..0000...../00009900000000.000/000000000///0;//////..-.-.9/////.//////////////////..9..///9.;.-.//.-.///.9//////////// @M03555:476:000000000-CJGL9:1:1102:7779:16008 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCGCCGCAGCCCAAACCTGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGCACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAACATTCCCTTTCTCTATTTGCTCTGTCTTCCTTTTTCCTTCTTCTTCCTCTTCCCTTTCTCTTTTACCTTCTTCCCTCTGTCCTCTCCGCCTGCTTTGTCCTTCCTTCTTCTCTTCTTTTCCTTCCTCTTCCTTCC +

A>1>FFFFBFFFG1FGEE00AAEA?EEHCHHC0B0BGB//GGEECECBGG000>/GA0BDG1FBGF1@E B>/>F11FFE//?@B@>FFGFGF1EE@GEF21FEG1B11 <B///?/1112?1?1?1111?111?11111111<1111110111>10=0000<0000=/=0000<000000000/0000/;//00:0000//....../000;00000/00;9000000;/99///9//9//;:////// @M03555:476:000000000-CJGL9:1:1102:25339:16354 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCACCAGGGACACCAGAGGCGCCGGAAGGCGACGGCAAGGCAGCCGACCAGACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATACTATCTGCAAATTACAAACAGACTATCCGCTACGTGTTACTTGTCACTTTGTCGCAAACACTCTTATCCTTGTCTGATCTGTGTCGTAAACGTCGA +

AAAAAFBB11BFFG1GA11BCFEF00FAF0GH000BEEE/E/A//A//EEE//>F//0//F/>EE?FHG//<///<<FC/B@CFFHHGHGHE /FFFGFHHHBGGHCE<AED<11.GGC.=0=0CECF/0D@:-.CGCFFFGC.;CGA@9-- ;.000000//;;/9//////9---9-/////--------/-/////:;//;////;---9-9--///9////9;//9///////////-----/--;9- @M03555:476:000000000-CJGL9:1:1102:25308:16554 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCAAAAACAACACGACATGCCACTTCACCCCCCGCGCGGCGACGCAACCACACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCTTCTTCTGCTTGAAAAATAACTTTCCTTTTCCATTCTCTTGACTTTTTCACCTGCTTATCTCTTATCCCTTCCACCCCACTCGCCTGCTACTTTATGTTTCCCTCCTTCCCCTACTTCTC +

A1>AACFB1CCF1BEGF?F1D00EE0ACF0A?E01BB1AB1BD1FHCAE//>EE////>/>/<ECE/<B<<B/B/</FB///<FHGCFCFA0GGDF1FGEEFFFF-C.CA0D00.</..;0:;..::/000<0<;0;09BFB.009..00000009;CFB0009;;000;00;;000;//////-////9//;//;B//:/;9//9-9---/-99---///;/////;//;///-;;///:9:--///// @M03555:476:000000000-CJGL9:1:1102:28345:16634 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCACACCCCCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACTGTCCGATAGATCTTATGAACGTTTCATCACGAGGTTATGTGTTACTTCATTGTACTCCATTGACACTCCTACGCTAACTGTGGCCGTTCATTGGCGCATATCATGGTTAGCTTAGCTAGTCTACTCTTTCTATTGTT + AB?A3FF@CC4B4BA4F ?EFGEFGGCEAEAEFEEHGGGGGHGCHHHFHBGCGHGEHEFGFH2>?GGGHFGHGHGHGD4BG1?EGFFHG?@A?FGF4GGC0FD3?C?>/////11<11...-<00000000000..///00000..--./00000;/00000000000000//00000000//900.-...///;/...-99./;////-.-..///////;/./////////////////;////9//// @M03555:476:000000000-CJGL9:1:1102:28685:17352 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCGAAGCGGAAGGAAAGGGGGCACACGGGGTCAAGCGCACACCAAAGCCCCACGAGAGAGCCGAAGCAGCAGACCACGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGTAAAAAAACTACCTATTTCCTTTCTTTTATCTATATTCCCTTCTTCTCTATTCTTCTTCCCCCCCCTTTCTTCTTCCTCTTTCTGTACCTCTCTTTCCTCTTTTTTCC +

1>AACC11CFFAG?GFEE0AEGG?00EEAGG0BAEGGGCCGGGGCG/FGHBEGEEEFGGAGHBGHE?EGCE/E/EHGGG/@/F0FGHE0C<////?FGDGHFHCCF<AFFGFF.<0..1>FHFFEC/DDCGC..=0GFFCC.;:00;--.00//000009;0C90;09009;C0;000;00;099;0009/9/9B/9//9/----9-99/BB///////;////:9///://////////999///-// @M03555:476:000000000-CJGL9:1:1102:7823:17784 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCCGCTCCGATCGCACCACCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAATTCCTATTTATCTGCATTACCCTACACTATTCCCCATTATGCGCATTGCATCTATTCTATACTCTCATGGCAGCCTCCACCCCATTCATCCTCTCAGAGCTGTTTCACAGCCTATTTGTTGCTTTT + A@AABFBDFFB4AFEFGGEGCG2EECFGG ?2AA2BAEEEAAD?AFEEC?ECFG1FGHFBGGFHGHGHFFHFF?FGGHFHFGFBCH/44BFCCE3FDDEEGHFGFFFDDF?GHFF//>@/-----./000000000:000000000/0/9//0/00000/9/00000..-..000;0000000////////9///./..........-../://///;////;9/////9///9/.../////9..///// @M03555:476:000000000-CJGL9:1:1102:8255:18146 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCCACCGCGGAGGACGCGCTCGGGCCGGCAAGGGAAGAGGCGAACCACAGCACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAACAAAAACCTTCTCCTTACTTGTGTCTGTCGTCGCTGCTACTCGTTCGACTGGTCCCTTGCGCTACGTGTATACTTTTTTTATGCCGCTTTACTCTTCGTTTA +

BAABBFCBBFFFGGEGGGFBFEGGGGCGGCFEGGGGGCEFCEGGGCGGGGEFGGHHGEEFFGHGHHHHHGDCDFFGHHGCGCCHFGHHHGCGEAHFHHHHGGGDHGEEGFD<DHC/F.EG0CGAEGHGHHGDGFFFFFEFFB.CB0.;......:.9//9//////9////;/////.9.9-.../////..9.....;.9//.//.9--9--.;.9.////;//9--9///9---..///9///.9/.. @M03555:476:000000000-CJGL9:1:1102:13216:18490 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCAGCAAAGTCCGAGAGAGCCGTAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAACAATATCTCCCTCCTTCACTCCTCCTTCCTCTCTCCTCCTTCCTTTTCTTTTTCCTTCTCCCTTTCCCTTCTCCCCTTCTTCCTCTCTTTTCCTCTCCTTCTCTTCTCTTCTCTCTTTCCCTCTCTTCCTCCTCTCTCTCTTCT +

AAAAABBB1BFFGF1FGGCGFG01BGGDFA000FECGG/AECEACF00AACC0FGDG21EA1AGEFFGHGHHG/B0/BFFF1FFFF02F/EEHGFHEEG1F01>1B///1122221000/<<1112<<D0B01101110110@00 @1101221111<1101111111?<111111>01110.0./0=00000/0<0000000//0:00000<:00000000000;00;///00;00;///0/00000;00 @M03555:476:000000000-CJGL9:1:1102:10818:18757 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCCGGGGCACACCGGTAGCGCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACTGCGCTTTGAACAAAGATGTAAGTGCATCAGCCTGAAGTCCGTTAATGATTACTACGCGTCGTCTAGTGTTCCAACCATCGATTGAAGTTAATACTCATACAGATTTGTAGATGACTTCTAGTCACCT + BAB@AFF4CFFFFGFGFCGGGGGCEDCGFGG2AEF?AEAFFEF?GHC0>@EGBCHHHGEFGF@F4B @FBGFGCEFEGHFEGGHHFAFF4?FG/BFCG2CADFGEGF2GFC0CB@CCGF ?--.000.--..00000/.//000:;000000;009//.900;00.;09.0000000;00.--9--;...////;/////..//./././;//////////:////////9/;/////////9///9/9//9 @M03555:476:000000000-CJGL9:1:1102:26867:19062 1:N:0:CTGTCCGTGA

GTGCCAGCAGCCGCGGTAATACGAAGGGACCTAGCGTAGTTCGGAATTACTGGGCTTAAAGAGTTCGTAGGTGGTTGAAAAAGTTGGTGGTGAAATCCCAGAGCTTAACTCTGGAACTACCATCAAAACTTTTCAGCTAGAGTATGATAGAGGAAAGCAGAATTTCTAGTGTAGAGGTGAAATTCGTAGATATTAGAAAGAATACCAATTGCGAAGGCAGCTTTCTGGATCATTACTGACACTGAGGAAC +

CCBBCFFCFFFBGGGGGGGGGGHGFGGGHGGHHHHGFGGGHGGGGGGHHHHHGHHHHHFHHHHHHHGEHGGGHGGGGHHHGHFHHGHEGDGGG?BBGFHHHGHHHHHHFHHHHHHHGHHHHHHHHHHHHHHHHHHHHHHHHHHFHHHHHHHHHHHHHHHHHHHHHHHHHHGHHHHHHGHHHHGHHGHHGGGHGGHHGHGHHHHHGHCCHHHFGCGFGGGGGGGGGGGGGGGGBFGGGGFFFFFFFFFFFF @M03555:476:000000000-CJGL9:1:1102:16446:19126 1:N:0:CTGTACGTGA

TGGCTCTGGCTTCCGTCCCGCAGACTGACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACACGTTTACTTTGGCTTTGAATATAATGCTTTCTTCTGCCTGCGTTATGCTGTATAATGTCTGGGGGCGACGTGTCTGTCTTGCATGTCTTGTCCCTTGCTCCATAGTCTGTGTCTGTATCACCTGTCTCGTGTTGTC + BB@BBFFFFFF @4AEFGFGCGGFCHFHBFFGGCEFGFFGCFEGHHHHGFFHGCGHHGGHFGHGHHGGFFHFGGFCGHGEGFBGF?EEFFFFGGFGFH44GGGAGBFHCC/@<//0/00?/1<1@101100111

<111111111111>1<=0000.-<..:0000;0;000;0000//--------././//;/;/9/9////9/////9/.//////////9/:/;/9F//////.;/9////9.99../ @M03555:476:000000000-CJGL9:1:1102:12049:19557 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCCAAAGCAACAGCCGCGACGGAAGAGCAAGACGAACAGCGCCGCACAAACCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGAAAGAGGAAGAACACAGCAGACGCGTGACTGCGCTGTAGAGTAGGGGATAACAGAAGAGGCAACAAGAGTGGACGGGTAATAAGTGTACGCAACG + ABABABBDFC4>FBECAECFFACFH4BGHFGECGGGCCEDGCABHHGFFFCGGGHH?CCGGGGGGAACE/@EECFDFFF?CGGFFHHFFHHEFGGFHGHHHHHGF@FADFB >GFGFBEEHBFGFCGHF=DC<AAAAFFF44CFBFGGFGGGGGHGGGCFHHHHHHHHHHHGFGHGGGHHHHHHFHHGHHGG5HHHFGHHHHFGGHGGGGGGHHHHGHHHHHGFHFGG3?GHHHHFFHHHFHHHHHHEGGGGGHHHGGHGGHHGGGGGHGGHFGGGGFHHHHGHHHHHHHGHHHHFGGGG?E0DHGHHHHHHAAFF4CCFFBBEGGFEGGG2AHBEEFC2BBFGA2E2EGAFFC0AE0CFFFCFFGBGGFHFHFB3BGHEE?CEHG@FBAGFCFBBGEEFFHGBGCE @GFHDEHHGHCCGF0//////<1111<10<10<11<1F1111.000<0<.---...;/00:00000000/./0.:/0/000900.....0000000000...///9///////////////9///9///..9./9./..---.9.;9//... @M03555:476:000000000-CJGL9:1:1102:14305:20752 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCCACGGCAGATCGACCAAGCTGCCAGGCCCGCCAACGCGGAACCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTCCTGTACGTGCATATCGTATGCCGTCTTCTGCTTGAAATAAAATTACATTCATGTTGTTATTCTTTCAGTTTTCTCACTTTTCTGCTTCCTGCTCTTCTCTCCTCCCTCTGTCGCATTTCGTCACTTTTGTCCACCCTTCCGTTCCTTTTC + AAA11CF1@1CBFFEGAE ?1FE000A0F1B0/A//FE11F01B//F/AEE?/FEFE??@E>//>>///?0E<GDH>GFG///??D<011222@G22221101111111111111100--- ..10000>AAAFFAFFFAGGGGGGFGGGHGEEGGGGGGGHHGHHHGHHGG1?FHBFHGHHHGGGGHHGFGGGGFGGCGDG@BFGFDD ?GHHHEFHGDGHFFGHHHFGCGAHHDDDGHHGGDGGHHHHHHHFHHHHHGHHHHHHHHFFGGGGGFDEGFFGGEFGGGGGGGGGGGGGFFFFFFFFFFFFFFFFFFFFFFFFFFFFBFFFFFFFFFFFFFFFFCFFEFFBFFFFFFFFFFFFFFFFFBFFFFFFFFFFF @M03555:476:000000000-CJGL9:1:1102:12733:21216 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCCAGCAGGCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAACACATGTTTCGCTTTATAATTATATATAGTTGCTTGCCGTTATTCGATGGTTTATCCCTACGATTATCGCTTATCCGCTTATCGCTCTCGCTGTATTCTTGTTTTTTACCTTCTGATCTGTATGGTGCCTATTTTCTTCTCATT + BBABBFFBFFFF4FCGGGGBFGFGHEGG?2AE2FDHEE0AECBGCGFFEFHGGHHFHFGFFHFGGGGGHFHF1BGE1F4GFEE>B3@FEE ?GFBDD4FGB0??3B>//0/223333?//??32@222222222222@2@221 /1/////110111<<101=11111......=0.....000:....;;.::..0....9000000000//.-;000;////////////.;/////9//;///////// @M03555:476:000000000-CJGL9:1:1102:15990:21520 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCAAGCCCGCAGAGCGCCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATACGCCTCCCCATGTATATCTAGCGTATTTGAGTGTTCAGGTGGAGTTCTGGTAGCGCGGTGCTGATCGTCTACATCAGAAGGCACCCCTCTGACGCAGTCATCTAATTGGCCAATAACTGTCTCTGTGGCCC + AAAAAFF1C1FCGGG111A11FA0A0E00FE0E/AA/AA////0A/A/>G0B0BG0?GH01B2121@G @1B/?//F2FG2FHH/>212>>FCE021/EE0/2B1B1B1<1110BCG/011?/>F.<>.0<011><111000-...EGHEHCEEHGG2FHF1101BFH?E///01210//?/221@//0/?11111111?1?11111@11111 <11111111>010=00/00000000=/<00000./:.:0;;../0.00090../.-/0;0..-..00000000000;0000;/////-9/9/9/-//9/;----// @M03555:476:000000000-CJGL9:1:1102:11792:22643 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCGGAGACACGCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGGAAAAAAAAATAGTCTTTTCATTGGTTATCGATTAATAATTAATACTAGGATTTTGTCGAATTTGTTCTATATTTTGATATTGGTAGTTTCGTTCTACTAGTGACGGTGGTGTCGTAGTTAATCTTGTACGGACGTTGTCTAGTCTTCG + AAAAAFF11B1C1FEGGGC000A0BEEAAAEE?FACFA/A/EBBBF00>>F/GFFHFGD1GFHHEF/EGGFFFHGGC/2FGGHAEHE2FEEGHGG2F11BF/11B00////01?11111<11<1111/111...1.111=>1111=0000/0<0000/0<..:.;00/0<0<000;;0.000;:0;009;00../.;00000;000.-9.99../-----/////:;////--------;//////9//- @M03555:476:000000000-CJGL9:1:1102:25889:22850 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCTCGGACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGCAAAAAAGTAAACTATCACAGTTTGAAATTTCAAAATACAGTGTGACATATATTGAGCGCCACTCCCACAAGTCTATACGTACTGAGTCTGTGCCACATGGAGTTTCTAAGTTGCTACTGGATCCTCACCCGTGTGCCCTTGTAGCGTCGATA + BBBABFFFFFFFFF?4GEEGGF2EEAEFGFEGGC2AEEBBCFHFGHH1FHHHHHEGFHGC@ ?FFFHHFFHBCFCFGHDGFEHHGGGE/FFGHF4FGE/?43??//34441B12B2211122111121@ @22><11111212111111?1<111?1/>-.<1>0.00.<.0000000.<0..00:00000<000:.C0/0///00000009;B00000//09/000...-....00///.9//:-.9--.. @M03555:476:000000000-CJGL9:1:1102:9997:23640 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCAGAAGCGCAGAGGCGAGGGACCACCGGGGCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATATCGTATGCCGTCTTCTGCTTGAAAAAACAACACTCCGCTCTTGACTATTACTTAAGACTACCAGTGCATCTTCTCTTACCAATTATTCACCTCCAGCTCACGCCTCCATTTACCCCTGACCTCTTTTTGATTCGTACACGTGTATTGTAT + BBAAAC>44CCC4GEGG?EC4FH4AAAA2FFEGGGGCGGCFHCEECEEE?/>>FEGHEEEEGHFGHHGF/B/GHHGHHHGGFGHGGHGHHHFHEFC//11<1?FFG<1<@ECHGGHBFDGGCCF0.-<:....0//...-.0000000000000000090//00900900000000000/0//;////9///.9./////:...9...;//////..9.///////9/-.///./.../.9.9.;///// @M03555:476:000000000-CJGL9:1:1102:26396:24009 1:N:0:CTGTACGTGA CTTTAGCGTTAAGGTACTGAATCTCTTTAGTCGCAGTAGGCGGAAAACGAACAAGCGCAAGAGTAAACATAGTGCCATGCTCAGGAACAAAGAAACGCGGCACAGAATGTTTATAGGTCTGTTGAACACGACCAGAAAACTGGCCTAACGACGTTTGGTCAGTTCCATCAACATCATAGCCAGATGCCCAGAGATTAGAGCGCATGACAAGTAAAGGACGGTTGTCAGCGTCATAAGAGGTTTTACCTCC + 1AAAABD1A>>1BBB3FFGF1FFGFHHHHGBGEEEEGFHGFGGGEGBHCEEEEFBAEEGGEFHGE1FEDHBGGHF1@FGBFHD001 >FBC0FGGFFA?E/ECB<01G1FFGFGFHBHHFDCHHGHBGBFC/CCCHFC1>GHHEAGHHBCCE@CGCGGEHHGHGGCGHHCB0 :C9;BFBFE90C.BFBBF9A9.C0CBBBFG@-@--F///;BBFFFFFF@ @BE99;/FBBBBFFB/BB9A:BFEFB/BFE @M03555:476:000000000-CJGL9:1:1102:10153:24102 1:N:0:CTGTACGTAA ACCTTTAGCATCAACAGGCCACAACCAACCAGAACGTGAAAAAGCGTCCTGCGTGTAGCGAACTGCGATGGGCATACTGTAACCATAAGGCCACGTATTTTGCAAGCTATTTAACTGGCGGCGATTGCGTACCCGACGACCAAAATTAGGGTCAACGCTACCTGTAGGAAGTGTCCGCATAAAGTGCACCGCATGGAAATGAAGACGGCCATTAGCTGTACCATACTCAGGCACACAAAAATACTGATAG + 3ABBBFFFFFFFCFGGGGGGGGHGEGFHGGGHHFHGHHHGCHHGHGGGGGHHGGGGHGHGGGGHHHGGGGFHHHHHHHHHHHHHHHHEGHHHGGGHHHHHHHHFDHHHGHHHHFHHHHHGFGGGGHGHGGGGGHGGGGCGGGHHHHHHCGHHHHHHGGGGGGGGGBFGGBFFGGGGGGGGFFFFFFFFFFFFAFFFFFFFFFFF/FFFFFABFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFBBFF/ @M03555:476:000000000-CJGL9:1:1102:10133:24116 1:N:0:CTGTACGTGA TGGCTTTGGCTTCCGTCCGGCCGCAGCGAAGGCGGGATAAGCGAAGAACCAGCACAGGCCAAAAGGGACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACAATCTCTTTCTTTATGAATAGTGCTCATCATGTTATCCGTATGACAATGTATACGTCAATTCGCCTTAGTGTACTCCAGACGTCAACATTAATACTTG + A>A?A11111@C1BAA11E ?AE0000//A//AGC/?//BFDD>E/>>0E>0/FC>>0/C/FG0/F/EGAECB?/F/FAC>//@CF0CG0FGFC0FGDD0/////-01111111111=0000=000/..<..00000/0000.///:::0000000;0000000000099//.0099B009//;9//..-./////////9///////9..--.;.//////./:////9/;//.;//9//// @M03555:476:000000000-CJGL9:1:1101:9719:2806 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCCACCCAGGGCACACAGGAGGACCCCAGCAACCCTAAAGGAGGAAGAGCCACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATTTCTACTGTCTAGAAGGAGAGAAAGCAGTGTGTGGTCGCTTTGTAGCCCTTCAGGCACGCAGCTCATACTATTGGCTCGGTGCTGCTGAGATTTG + CCBCCFFFFFFFCGEGGGGGGGGGHGGGGFHGGHGAFGGHHGGGGHFGHFGHHGHGHHEEEHGHGFHHHGFCEEEFHHFECCGHGHHHHGHHHGGFHHFHHHHGF@FGFFHFFHGHHFAG3FGH ?DHGFGGC?GEHHHHHHG0?1DAC@ ----;./000000000000000......//////9/00:..:0--..:B/////////////...----//////////:////--;.-//////./;/// @M03555:476:000000000-CJGL9:1:1101:17722:3693 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCGAAACCCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAGTCTTTGATGAGACACATTTTCTGCTCGATGCGTACCTTTTATTGCTGCCTTTTCAACTCGTCTCTGGCGTGTGGGACACTGTGCTGACGCGAGCGTGTTTCCTCGGTCCACGTGTCTTCGAGTGTGTCCTGATGCGT + ABBB3FFFFFFFFGEGGGGCEEFHGFFEGCGGGEHG?EEFFHHHFFGGHEHHGHHHHHHHHHGFHGHHHHHHHHHEHBHHH1EHHGHGGGHGFE4FHHHGBGHG////A>>1C1@F1FFBGGAFAEGAE0E00EEECEGCHF0FF0AFG /FGGHBGBGGHGHGGHGHF2FGEFG/?G1BFC/>B021@ />FFBGHHFHB>112BFFBEB2202///BB11212BB11BB002<10//00/11121111111?1110000111111=1111=1<11111>111100000000/000000==000000/::;.0/...-.0009/9/;000;00900000009/09.//;0000;/;/- @M03555:476:000000000-CJGL9:1:1101:21945:4229 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCAAGGCAACGACAACAGGGCGCGGCCCTAGACCGACGACGACACAAAGGGTCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACTAGTAGCCATATATTGTTGGAGTGATACTCCTTGCGTCCTTAGAGTCCCCCCATGTGCGAAGGGTCCACACTAGAACGTACCTTATGTTCAGGGCC + ABBB?FFBBCFFGGGGGGG5AAFFHHEDG2ACGHHGGGGGGGGGHHHBGFEEEEGGGGGGGHGA?BEHGGE@CDEGHGC @CCCB0B0?<0GBGAF1FGH1F1DF<@////>/?1111111111..-..../0<000</?1?1.011111=1111>>111<../.0=0000<00000000000<-:..../.;/000009;C0000;000;:B0.9::;../.0009/9.....///////;9.9F///;9B..//:// @M03555:476:000000000-CJGL9:1:1101:7510:5270 1:N:0:CTGTACGTGA TGGCCCTGGCTTCCGTCCCACCGACCCCGCGAGAGAGCCGAAGCAGCTGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGCTAAAAAAAATCTCTTCCATCAACTCTTTTCAGCTATCTTCTTATCTCTTAACGCTGACTTTCTAGTTTCTGGTTGCCCTTCGTCTATTTTCGACTTCTACCCCATTGCGCTGTTTGCTTTCTTGATCGTGTCTGCCGCTGCATAAC + AAAA1>A?1>>C1GGGF1E1FFGCG?EAEE//A///E/0E/A>/@000B@0B @?0B1B1EG11F1BE//>FGHE@/FCEFF1G1>//BE2B@ />BFGD11>1F/1BBF11///01111?<1>GFH111>>11>>11<<0=0000=0==.<.../0;:G0;000<;000:;..99;/:./.;.;0;00-...;0000;.9./9//----9////:9///BB///-9---9///-;9--////9 @M03555:476:000000000-CJGL9:1:1101:9289:5390 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCCGAGAGGTCAGCCTGCCGCGACCCGCCCAGCACCCACGCGGCCGCACAGACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATGTCGTACAGCTACTGTACTAAAAACTATACCGTAGTCTGAAAAGTCGAGTTACTGAATCATGTGATCAGTCTTAACGCTTGAGCTGTTGGTGATAC + BBABBFFFFBBCBFEGGGGGGGGGGHBGGGHHFFHEECEEGGGGGGGHHHHHEGGGGE?CEGGEGGFEHGGCGCEDFHCC?@CHFFHHHHHEHGFBGHHGHGHFFGFCFEHHHHEHH..DAAB3BCBBBCCGGGGGGCGGFFFFFGGGGGGF2EEEHHHGGFGFGGGGGGGGCEEEFGEGGGEFEFHHFHHHHHGHHHHGFDGGGHHGGFGFH?@FGFGEGD1?GHFDGFHH@ <@0<>FFHHHG0EGFHEEGHGHHHHGFFCGHGHGCF/><--.00=00000/.0000<0=000;0;000/0///0000;0:C-----.:/090000000000000000./9..///9///...../;//////.-..9/..:..//9./;/9/9/9B//9//9//:../:-. @M03555:476:000000000-CJGL9:1:1101:20011:5687 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCGTCCCACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACCCTTGCCCTGCCTTTAAAACTGTTAGTCTTGCGATCGCGAGTGGTGAGTGGCCTTCCACGTGTCGATGTGAAATTCGTCGTTCTTTGTATGACCCCCCTTTGCTGCCTCTGCTCACTGTCTCGCTACTGACGCTGAGGTCC + A1A1>CF1BCCFFGGFGGEGGG?1GE?E0E00C0/////10B0FGH/0F0F1FEDG1EGHF?F/EEGHHEH0F/BF1@F0B /B02BE?/BFGF1FF110B110////////0?011@ @0?01->F-.<-<-;.<.<:C0:..:C0/;09C.9/..9;:C000;;BABA@ -;.;9BB/////99-;----;/////-/;BBFFBBFB//;B9/---@F///-99----/-9 @M03555:476:000000000-CJGL9:1:1101:20081:5711 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCACGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACTACTATTTATTTTTATTTTTGCCATTACTTGTACACGTGTGATCCTTCTCGTCAGTTTTATTTGTTACCTGTACCCCTTTATCGCTACTTATGTGTGCCTCACTATGTTTTTGCTCTTTATTTATCACTCTCTCTTCTTCTTGCT + CCBCCFFFFFFFGGGGGEGGGGGGGAEHGGGGGGHHHHHHHGHHAFGHHHHHHFGGFGHGHHHEHHHHHGHFHGHGGHHFHCFEHFHHHEGHH?F3DEFF?E<1/11111<1?111111<<0.<.11<111110.....<000<00000/00000;0000...:0;:....-00000900.000:/9090;000/.-00000://////://////:///;/9/:9/// @M03555:476:000000000-CJGL9:1:1101:22096:5851 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCGCACGAAGTGGCCCCCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAATGTCTAAGAAAGTAAAACCTCTAGAGATTGTAGAATTGCACAAATTGTGATAGTGTCTAGTATGTTATCATCATCCATCGGTTACAGTAAGCGAGTATGATGATGTCGAGCTTACAGAAGCGTTGGG + BC@ABFFFFFFFGGGGGGGGGGGGGGGHHGHHGGEGGGCFDFGHGGGCEGFHHFFHHHHGHHHHHFGHFHHGEEEHHHGHECG ?FG?FGFFGHHFHGFGHGFHGGHHHECDF?/@/A?<-...<000000000//000000.//000000000;00000000000/.90000000000;0000000000000000000////.....//;:////-.--./////9////9::..///://///-..-.- @M03555:476:000000000-CJGL9:1:1101:18560:5961 1:N:0:CTGTACGTGA TGGCTCTGGCTTCCGTCCCCGAGAGAGCCGAAGCAGCAGACCAAGTCTCTGCTACCGTACTGTACGTGAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAATTGACTCTTTCCCTTCCATCACAACTTTTCAGCTATAGTATTAGACATGAAATCATCTTTTCTATTTTATAGTTGAAATTCGTATATATTATAAAGAATCCCAATTTCGATTGCACCTTTCTGTCTCATTACTCTCTCTTAGTAAC + BBBAAFFBCFFFGGGGF2E4AEEECAEA2AE2AEGHCFHB1F2AE5DFGFFHH5F2EFEEGFD551BFGAG5F@FG1FFFHCG @HFBGHHGHGFB3BB@FCD//0<22?>?2222@<@22<1@DG11 ?C11FD11<1<==G1>1<11111>0000000===0/////0223222@22 <22222@2 @<<1111?11111<.<>=100EG02FEB>G/2F1@BFF /FDE>///E//?111011?0101<11//0?/1111111111...--<.
drivenbyentropy commented 4 years ago

I have just taken your example data and parsed it with AptaSuite using the method I suggested and it works. Please follow the suggestions below and let me know if your can replicate it.

In the Create new Experiment wizard

Then create as many selection rounds as you have data and provide the Round Number, Round Name and the Forward Reads File for each.

Hit Next and Import Data.

samoudj commented 4 years ago

it workedddddd thank you so muchhhhh

Le mar. 20 août 2019 à 13:44, drivenbyentropy notifications@github.com a écrit :

I have just taken your example data and parsed it with AptaSuite using the method I suggested and it works. Please follow the suggestions below and let me know if your can replicate it.

In the Create new Experiment wizard

  • Select IS Demultiplexed (I assume you have one file per selection round)
  • Select Single End (Ignore the reverse reads for now)
  • As 5' Primer enter TGGCTCTGGCTTCCGTCC
  • As 3' Primer enter CGAGAGAGCCGAAGCAGC

Then create as many selection rounds as you have data and provide the Round Number, Round Name and the Forward Reads File for each.

Hit Next and Import Data.

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drivenbyentropy commented 4 years ago

You are welcome.

For future reference, you can check these things by yourself using a text editor (e.g. Notepad++) to open a sample of the fastq data and using the search function of the editor to see if you can find the 5' and 3' primer (or their reverse complement!) in the sequences. This will help you to see what information AptaSuite requires.