Closed akhst7 closed 5 years ago
Hi,
I created a Seurat-Single R object by using,
singler=CreateSinglerSeuratObject("~/filtered_gene_bc_matrices/mm10/", annot = NULL, project.name="test",min.genes = 200, technology = "10X", species = "Mouse", citation = "",ref.list = list(), normalize.gene.length = F, variable.genes = "de", fine.tune = T, reduce.file.size = T, do.signatures = T, min.cells = 2, npca = 10, regress.out = "nUMI", do.main.types = T,reduce.seurat.object = T, numCores = 12)
Aside from getting a warning, the singleR object was created and I then saved my singler as a .rds file and loaded onto the SingleR website but then I got a following ;
Any work around for this ?
Thanks.
Look at the main page in the Github repository. You need to do one more step - singler.new = convertSingleR2Browser(singler)
singler.new = convertSingleR2Browser(singler)
Hope this helps.
Thanks. It works.
Hi,
I created a Seurat-Single R object by using,
singler=CreateSinglerSeuratObject("~/filtered_gene_bc_matrices/mm10/", annot = NULL, project.name="test",min.genes = 200, technology = "10X", species = "Mouse", citation = "",ref.list = list(), normalize.gene.length = F, variable.genes = "de", fine.tune = T, reduce.file.size = T, do.signatures = T, min.cells = 2, npca = 10, regress.out = "nUMI", do.main.types = T,reduce.seurat.object = T, numCores = 12)
Aside from getting a warning, the singleR object was created and I then saved my singler as a .rds file and loaded onto the SingleR website but then I got a following ;
Any work around for this ?
Thanks.