dylanbeeber / crispRdesignR

Software used to design guide RNA sequences for CRISPR/Cas9 genome editing
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How to use a custom de novo assembled transcriptome for guid RNA design #9

Open Ram-Nagar opened 1 year ago

Ram-Nagar commented 1 year ago

Hi there, I only have a de novo assembled transcriptome of a plant species, and that is the only information is available. I have identified the target gene from the transcriptome, and now I would like to design guide RNA for editing. Wounding if I could still use crispRdesign for designing guide RNA? If yes, could you please explain how.

Thanks