Test works but binsanity-profile fails

[biotemon]

Binsanity-profile -i contigs_v2.fa -s /Volumes/Red_4TB/scratch_BK/CS_BACKUP/p31_anvio_metagenomes/OC26_EXPERIMENT/binsanity_bins/bam_files/ -c binsanity.profile

/Users/tito_miniconda/opt/miniconda3/bin/Binsanity-profile:152: SyntaxWarning: "is not" with a literal. Did you mean "!="?
  if args.outDirectory is not ".":

        ******************************************************
                    Contigs formated to generate counts
        ******************************************************

Traceback (most recent call last):
  File "/Users/tito_miniconda/opt/miniconda3/bin/Binsanity-profile", line 164, in <module>
    feature_counts(args.inputMapLoc, args.outDirectory, args.Threads)
  File "/Users/tito_miniconda/opt/miniconda3/bin/Binsanity-profile", line 31, in feature_counts
    subprocess.call(["featureCounts", "-M", "-O", "-F", "SAF", "-T", str(threads), "-a", os.path.join(outputdir, str(
  File "/Users/tito_miniconda/opt/miniconda3/lib/python3.8/subprocess.py", line 340, in call
    with Popen(*popenargs, **kwargs) as p:
  File "/Users/tito_miniconda/opt/miniconda3/lib/python3.8/subprocess.py", line 858, in __init__
    self._execute_child(args, executable, preexec_fn, close_fds,
  File "/Users/tito_miniconda/opt/miniconda3/lib/python3.8/subprocess.py", line 1704, in _execute_child
    raise child_exception_type(errno_num, err_msg, err_filename)
FileNotFoundError: [Errno 2] No such file or directory: 'featureCounts'

[edgraham]

Hello Tito,

I'm sorry you're having issues. This looks like a python related syntax error. I'll look into it ASAP and send out a repair. In the meantime try these things:

1. Try running the test dataset and see if that will run.
2. Try dropping your conda env to python 3.6 and re-running this command.

-Elaina

On Mon, Jan 17, 2022 at 7:16 PM Tito Montenegro @.***> wrote:

Binsanity-profile -i contigs_v2.fa -s /Volumes/Red_4TB/scratch_BK/CS_BACKUP/p31_anvio_metagenomes/OC26_EXPERIMENT/binsanity_bins/bam_files/ -c binsanity.profile

/Users/tito_miniconda/opt/miniconda3/bin/Binsanity-profile:152: SyntaxWarning: "is not" with a literal. Did you mean "!="? if args.outDirectory is not ".":

    ******************************************************
                Contigs formated to generate counts
    ******************************************************

Traceback (most recent call last): File "/Users/tito_miniconda/opt/miniconda3/bin/Binsanity-profile", line 164, in feature_counts(args.inputMapLoc, args.outDirectory, args.Threads) File "/Users/tito_miniconda/opt/miniconda3/bin/Binsanity-profile", line 31, in feature_counts subprocess.call(["featureCounts", "-M", "-O", "-F", "SAF", "-T", str(threads), "-a", os.path.join(outputdir, str( File "/Users/tito_miniconda/opt/miniconda3/lib/python3.8/subprocess.py", line 340, in call with Popen(*popenargs, **kwargs) as p: File "/Users/tito_miniconda/opt/miniconda3/lib/python3.8/subprocess.py", line 858, in init self._execute_child(args, executable, preexec_fn, close_fds, File "/Users/tito_miniconda/opt/miniconda3/lib/python3.8/subprocess.py", line 1704, in _execute_child raise child_exception_type(errno_num, err_msg, err_filename) FileNotFoundError: [Errno 2] No such file or directory: 'featureCounts'

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[saras224]

Hi @edgraham I also got an error while running binsanity_profile command. Command: Binsanity-profile -i A1.fa -s /home/saraswati/MAPPED_SORTED/SORTED/BINSANITY_TRIAL/ -c binsanity_coverage

error message: /lustre/saraswati/anaconda3/bin/Binsanity-profile:152: SyntaxWarning: "is not" with a literal. Did you mean "!="? if args.outDirectory is not ".":

    ==========     _____ _    _ ____  _____  ______          _____  
    =====         / ____| |  | |  _ \|  __ \|  ____|   /\   |  __ \ 
      =====      | (___ | |  | | |_) | |__) | |__     /  \  | |  | |
        ====      \___ \| |  | |  _ <|  _  /|  __|   / /\ \ | |  | |
          ====    ____) | |__| | |_) | | \ \| |____ / ____ \| |__| |
    ==========   |_____/ \____/|____/|_|  \_\______/_/    \_\_____/
  v2.0.3

//========================== featureCounts setting ===========================\						Input files : 1 BAM file
A1.sorted.bam
Output file : A1.sorted.bam.readcounts
Summary : A1.sorted.bam.readcounts.summary
Paired-end : no
Count read pairs : no
Annotation : A1.sorted.bam.saf (SAF)
Dir for temp files : .
Threads : 1
Level : meta-feature level
Multimapping reads : counted
Multi-overlapping reads : counted
Min overlapping bases : 1

\============================================================================//

//================================= Running ==================================\						Load annotation file A1.sorted.bam.saf ...				Features : 35630				Meta-features : 35630				Chromosomes/contigs : 35630
Process BAM file A1.sorted.bam...

ERROR: Paired-end reads were detected in single-end read library : /home/saraswati/MAPPED_SORTED/SORTED/BINSANITY_TRIAL/A1.sorted.bam

    ******************************************************
                Contigs formated to generate counts
    ******************************************************

Traceback (most recent call last): File "/lustre/saraswati/anaconda3/bin/Binsanity-profile", line 166, in make_coverage(args.inputFasta, args.outCov, args.outDirectory) File "/lustre/saraswati/anaconda3/bin/Binsanity-profile", line 54, in make_coverage contig_length = cov_data[k].pop(0) IndexError: pop from empty list

real 1m37.531s user 0m54.310s sys 0m1.387s

Kindly help me resolve the error!

Thanks in Advance!