Closed wrightalecm closed 3 years ago
hey @wrightalecm,
You have to supply a valid pin name (or path) to active_snw_search()
(see [here]:(https://egeulgen.github.io/pathfindR/articles/manual_execution.html))
active_snws <- active_snw_search(input_for_search = RA_processed,
pin_name_path = "Biogrid",
snws_file = "subnetworks",
search_method = "GR")
Let me know if you get an error with this calll.
If still getting an error, please make sure that the library is loading properly (i.e., finding the Java installation) and please share the output of sessionInfo()
after loading the library
Yes, this appeared to do the trick! I really appreciate you taking the time to answer. Feel free to close this thread.
Thank you, Alec
Hello @egeulgen ,
I have a similar issue while running the package. I have the java installed already and Rv4.0.5. I executed run_pathfind(RA_input)
and manually using
active_snws <- active_snw_search(input_for_search = RA_processed,
pin_name_path = "Biogrid",
snws_file = "active_snws",
score_quan_thr = 0.8,
sig_gene_thr = 0.02,
search_method = "GR")
I can see a directory created active_snw_search with input_for_search.txt in it. The error message says :
Error in pathfindR::filterActiveSnws(active_snw_path = snws_file, sig_genes_vec = input_for_search$GENE, :
The active subnetwork file does not exist! Check the active_snw_path
argument
In addition: Warning message:
In file.rename(from = "resultActiveSubnetworkSearch.txt", to = snws_file) :
cannot rename file 'resultActiveSubnetworkSearch.txt' to 'active_snw_search/active_snws.txt', reason 'The system cannot find the file specified'
file.access(".")
gives 0.
Could you please help me resolve this issue so that I can proceed with the analysis.
Thank you Neha
hey @neha163,
it seems that the active subnetwork search results are not written to "resultActiveSubnetworkSearch.txt".
do you mind running file.access(".", mode = 2)
? This will test for write permission.
Hello @egeulgen ,
The file.access(".", mode = 2)
says 0.
hello again @neha163,
This might be an issue with R not being able to find your java installation. Running the command below (from the terminal) might resolve the issue.
R CMD javareconf
if you're comfortable with debugging, running debugonce(active_snw_search)
and running your code again should help pinpoint where the issue lies.
Hello @egeulgen,
Running R CMD javareconf
on terminal shoots an error of R CMD javareconf. I am not familar with the debugonce(active_snw_search)
. I takes me to the function internal codes which I am not confident to making edits. Is there any other solution to resolve the issue?
what error do you get running R CMD javareconf
on the terminal?
You do not have to make edits when debugging, just run line by line and see for which command you get an error. Unfortunately, there is no other way, as this seems to be an issue related to java configuration on your computer.
Hello @egeulgen , I am sorry for the prior typo. It gives error- 'javareconf' is not recognized as an internal or external command, operable program or batch file. Also I will check with my java configuration. Thank You for your help. Best Neha
Indeed, installing the Java Environment (even Java 18 SDK, latest at the time of writing this comment, seems to have solved the issue).
Hello, I saw someone else had this error and I followed along your suggestions but unfortunately I still am having the same issue.
To Reproduce Steps to reproduce the behavior:
2a. Run the following function: library(pathfindR) output_df <- run_pathfindR(RA_input)
3a. There is already a directory named "pathfindR_Results". Writing the result to "pathfindR_Results(2)" not to overwrite any previous results.
Testing input
The input looks OK
Processing input. Converting gene symbols, if necessary (and if human gene symbols provided)
Number of genes provided in input: 572 Number of genes in input after p-value filtering: 572 Could not find any interactions for 5 (0.87%) genes in the PIN Final number of genes in input: 567
Performing Active Subnetwork Search and Enrichment
Error in { : task 1 failed - "The active subnetwork file does not exist! Check the
active_snw_path
argument"I then tried manually as you suggested in troubleshooting: 2b. processed_data <- input_processing(input = RA_input, p_val_threshold = 0.05, pin_name_path = "Biogrid", convert2alias = TRUE)
active_snws <- active_snw_search(processed_data, pin_path, snws_file = "subnetworks", search_method = "GR")
3b. Error in pin_name_path %in% valid_opts[-length(valid_opts)] : object 'pin_path' not found
I checked to make sure I have writing privileges as you suggested: file.access(".", 2) Output: 0
There is a directory created called "active_snw_searches", however there is no directories "input_for_search.txt' or "subnetworks.txt"
Expected behavior Output of run_pathfindR
Desktop (please complete the following information):
R Session Information: R version 4.0.4 (2021-02-15) Platform: x86_64-w64-mingw32/x64 (64-bit) Running under: Windows 10 x64 (build 19042)
Matrix products: default
locale: [1] LC_COLLATE=English_United States.1252 LC_CTYPE=English_United States.1252
[3] LC_MONETARY=English_United States.1252 LC_NUMERIC=C
[5] LC_TIME=English_United States.1252
Additional context I did initially have some issue with loading the library as I was getting an error that I didn't have Java installed even after I had just installed it. I was able to solve this issue by using:
Sys.setenv('JAVA_HOME'="C:/Program Files/Java/jre1.8.0_281/")
system("java -version") [1] 127
Thank you for any help and please let me know if you need any more information! Alec