Closed qverron closed 9 months ago
Hi! I added this feature to version 0.1.5.
The corresponding *--help** section looks like this:
--SpaceTx
Save one image per z-plane according to the SpaceTx convention.
<image_type>-f<fov_id>-r<round_label>-c<ch_label>-z<zplane_label>
<image_type> will be the name of the nd2file (without extension)
<round_label> will always be 0.
Marking as solved. Please reopen if you'd like any revisions.
. Add flag to split nd2 file into single 2D planes instead of the current 3D z-stacks.
. Add flag to format split data according to SpaceTx convention:
The minimum time_types are "primary" (FISH imaging rounds) and "nuclei" (DAPI). We could attribute all FISH channels to "primary" with the different colors encoded into the -c variable. It would be great to fetch metadata to create the physical coordinates csv file at the same time.
ref: https://spacetx-starfish.readthedocs.io/en/latest/gallery/data_formatting/plot_format_structured_data.html#format-structured-data https://spacetx-starfish.readthedocs.io/en/latest/help_and_reference/spacetx-format/SpaceTxFormat/index.html#sptx-format