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elolab / glaDIAtor

Tool for processing metaproteomics DDA and DIA data.
GNU General Public License v3.0
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glaDIAtor shut down at log: /opt/comet/comet-ms -P/run-files/analysis xxx #1

Open andrewyy677 opened 2 years ago

andrewyy677 commented 2 years ago

Dear Sami Pietilä,

Thanks for excellent software for metaproteomic DIA data! I met a problem when running glaDIAtor on Windows 10 platform from web browser. My input rawdata was one of your example rawdata named 170825_HF_1ug_DIA_1column_5.raw, and my fasta files were IGC_MLI28_fungivirus.fasta, irt.fasta, and contaminant.fasta. The web interface was the following picture and the log was also attached. Thanks for your time!

best, Yingying

log.txt image

sampie commented 1 year ago

Hi

Indeed, it looks like there is nothing in the log after the comet run analysis was started. Not even an error message. It looks like the container might have been stopped for some reason. Can you check if the container is still running? One possible explanation could be that system has run out of memory and process / container has been killed.

Unrelated to the current issue, there is only one mzML file provided. It might be a problem during later alignment steps.

andrewyy677 commented 1 year ago

Yeah, there is no error message in the log. I’ve checked the container and it’s still running. The RAM of my computer is 128 GB, which may not be enough for the process. I will give it a try in a larger RAM PC.

Another problem while pulling the glaDIAtor docker was that some parts were always showed “Waiting” and some parts were not 100% downloaded, but it showed “Status: Downloaded newer image for elolab/gladiator:latest” in the end (screenshot attached). I do not know whether it is because the internet speed or something, and whether the pulling is completed, and if it is the reason why the process stopped after the comet run analysis.

Thanks again!

Best, Yingying glaDIAtor_pulling

sampie commented 1 year ago

I guess the image might be fine as the pull command completed and did not reporting errors.

You could try to execute the comet step manually to see what actually happens. This can be done by first running "docker ps" to find out what is the container id and then going into the container "docker exec -it THE_ID /bin/bash". Then, in the container, go to your project folder: "cd /run-files/analysis-20220831-012053/" and finally run the comet: "/opt/comet/comet-ms -P/run-files/analysis-20220831-012053/comet_settings.xml 170825_HF_1ug_DIA_1column_5_Q1.mzXML" to see what is the output.

It is also possible to try to run analysis without comet and just use xtandem (uncheck "Use comet search engine" from GUI), if comet does not work for some reason.

andrewyy677 commented 1 year ago

Thanks a lot! I've try the second solution that use xtandem only. It could run normally so far (GUI snapshot attached). I was wondering what the differences between the results of using xtandem only and using both xtandem and comet are. Is it ok to use xtandem only to analysis the data?

Another question is that I found a warning message ("Warning: Only one FASTA input file was provided, which might not contain contaminants.You probably want to have them! Just add the contaminant file to the input file list 'in'.") in the 1500 line of the log.txt (attached), but I do input four FASTA files. Does the warning mean the FASTA files were not input properly? If so, what are the implications for the following analysis, and how to solve it?

Thanks again!

Best, Yingying glaDIAtor_20220915 log.txt

andrewyy677 commented 1 year ago

Thanks a lot! I've try the second solution that use xtandem only. It could run normally so far (GUI snapshot attached). I was wondering what the differences between the results of using xtandem only and using both xtandem and comet are. Is it ok to use xtandem only to analysis the data?

Another question is that I found a warning message ("Warning: Only one FASTA input file was provided, which might not contain contaminants.You probably want to have them! Just add the contaminant file to the input file list 'in'.") in the 1500 line of the log.txt (attached), but I do input four FASTA files. Does the warning mean the FASTA files were not input properly? If so, what are the implications for the following analysis? How to solve it?

Thanks again!

Best, Yingying

从 Windows 版邮件https://go.microsoft.com/fwlink/?LinkId=550986发送

发件人: Sami @.> 发送时间: 2022年9月14日 15:01 收件人: @.> 抄送: Yingying SUN @.>; @.> 主题: Re: [elolab/glaDIAtor] glaDIAtor shut down at log: /opt/comet/comet-ms -P/run-files/analysis xxx (Issue #1)

I guess the image might be fine as the pull command completed and did not reporting errors.

You could try to execute the comet step manually to see what actually happens. This can be done by first running "docker ps" to find out what is the container id and then going into the container "docker exec -it THE_ID /bin/bash". Then, in the container, go to your project folder: "cd /run-files/analysis-20220831-012053/" and finally run the comet: "/opt/comet/comet-ms -P/run-files/analysis-20220831-012053/comet_settings.xml 170825_HF_1ug_DIA_1column_5_Q1.mzXML" to see what is the output.

It is also possible to try to run analysis without comet and just use xtandem (uncheck "Use comet search engine" from GUI), if comet does not work for some reason.

— Reply to this email directly, view it on GitHubhttps://apc01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fgithub.com%2Felolab%2FglaDIAtor%2Fissues%2F1%23issuecomment-1246332181&data=05%7C01%7Csunyingying%40westlake.edu.cn%7C4311cc5e9a17413569eb08da961ef7a6%7C7e82de2f7ef644169b9644c1457be81b%7C1%7C0%7C637987356999763400%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=YTsfggSBvtUZDnp44cglodE7LFQbBSlw2T6b94YIwp0%3D&reserved=0, or unsubscribehttps://apc01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fgithub.com%2Fnotifications%2Funsubscribe-auth%2FA2UR3JM7FEGZBZ5SR236543V6FZ43ANCNFSM6AAAAAAQA7OWB4&data=05%7C01%7Csunyingying%40westlake.edu.cn%7C4311cc5e9a17413569eb08da961ef7a6%7C7e82de2f7ef644169b9644c1457be81b%7C1%7C0%7C637987356999763400%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=FHyK809WbFqWTx3KwrxHTspQ4j%2BR1tlUWxEEcMqPDSs%3D&reserved=0. You are receiving this because you authored the thread.Message ID: @.***>

Running pipeline command: /opt/tpp/bin/msconvert /data/170825_HF_1ug_DIA_1column_1.mzML --32 --zlib --filter "peakPicking false 1-" --mzXML -o /run-files/analysis-20220914-140152/libfree format: mzXML m/z: Compression-Zlib, 32-bit intensity: Compression-Zlib, 32-bit rt: Compression-Zlib, 32-bit ByteOrder_LittleEndian indexed="true" outputPath: /run-files/analysis-20220914-140152/libfree extension: .mzXML contactFilename:

spectrum list filters: "peakPicking false 1-"

chromatogram list filters:

filenames: /data/170825_HF_1ug_DIA_1column_1.mzML

processing file: /data/170825_HF_1ug_DIA_1column_1.mzML writing output file: /run-files/analysis-20220914-140152/libfree/170825_HF_1ug_DIA_1column_1.mzXML

Running pipeline command: /opt/tpp/bin/msconvert /data/170825_HF_1ug_DIA_1column_5.mzML --32 --zlib --filter "peakPicking false 1-" --mzXML -o /run-files/analysis-20220914-140152/libfree format: mzXML m/z: Compression-Zlib, 32-bit intensity: Compression-Zlib, 32-bit rt: Compression-Zlib, 32-bit ByteOrder_LittleEndian indexed="true" outputPath: /run-files/analysis-20220914-140152/libfree extension: .mzXML contactFilename:

spectrum list filters: "peakPicking false 1-"

chromatogram list filters:

filenames: /data/170825_HF_1ug_DIA_1column_5.mzML

processing file: /data/170825_HF_1ug_DIA_1column_5.mzML writing output file: /run-files/analysis-20220914-140152/libfree/170825_HF_1ug_DIA_1column_5.mzXML

Running pipeline command: java -Xms97g -Xmx97g -jar /opt/dia-umpire/DIA_Umpire_SE.jar /run-files/analysis-20220914-140152/libfree/170825_HF_1ug_DIA_1column_1.mzXML /run-files/analysis-20220914-140152/diaumpire-params.txt

DIA-Umpire singal extraction analysis (version: v2.1.3) 2022-09-14 14:07:25,705 INFO [root] Version: v2.1.3 2022-09-14 14:07:25,706 INFO [root] Parameter file:/run-files/analysis-20220914-140152/diaumpire-params.txt 2022-09-14 14:07:25,706 INFO [root] Spectra file:/run-files/analysis-20220914-140152/libfree/170825_HF_1ug_DIA_1column_1.mzXML 2022-09-14 14:07:25,710 INFO [root] #Number of threads 2022-09-14 14:07:25,711 INFO [root] # set to the number of cores available 2022-09-14 14:07:25,711 INFO [root] Thread = 5 2022-09-14 14:07:25,712 INFO [root] 2022-09-14 14:07:25,712 INFO [root] #Precursor-fragments grouping parameters 2022-09-14 14:07:25,713 INFO [root] RPmax = 25 2022-09-14 14:07:25,714 INFO [root] RFmax = 300 2022-09-14 14:07:25,714 INFO [root] CorrThreshold = 0.2 2022-09-14 14:07:25,716 INFO [root] DeltaApex = 0.6 2022-09-14 14:07:25,716 INFO [root] RTOverlap = 0.3 2022-09-14 14:07:25,717 INFO [root] 2022-09-14 14:07:25,718 INFO [root] #Fragment intensity adjustments 2022-09-14 14:07:25,718 INFO [root] # change BoostComplementaryIon if later using database search results to build libraries for Skyline/OpenSWATH 2022-09-14 14:07:25,719 INFO [root] AdjustFragIntensity = true 2022-09-14 14:07:25,720 INFO [root] BoostComplementaryIon = true 2022-09-14 14:07:25,720 INFO [root] 2022-09-14 14:07:25,721 INFO [root] #Export detected MS1 features (output feature file can be loaded and mapped to RAW data in BatMass) 2022-09-14 14:07:25,721 INFO [root] ExportPrecursorPeak = false 2022-09-14 14:07:25,721 INFO [root] 2022-09-14 14:07:25,722 INFO [root] #Signal extraction: mass accuracy and resolution 2022-09-14 14:07:25,722 INFO [root] # resolution parameter matters only for data generated in profile mode 2022-09-14 14:07:25,722 INFO [root] SE.MS1PPM = 15 2022-09-14 14:07:25,723 INFO [root] SE.MS2PPM = 25 2022-09-14 14:07:25,723 INFO [root] SE.Resolution = 60000 2022-09-14 14:07:25,724 INFO [root] 2022-09-14 14:07:25,724 INFO [root] #Signal extraction: signal to noise filter 2022-09-14 14:07:25,724 INFO [root] SE.SN = 1.1 2022-09-14 14:07:25,725 INFO [root] SE.MS2SN = 1.1 2022-09-14 14:07:25,725 INFO [root] 2022-09-14 14:07:25,726 INFO [root] #Signal extraction: minimum signal intensity filter 2022-09-14 14:07:25,726 INFO [root] # for Thermo data, filtering is usually not necessary. Set SE.EstimateBG to false and SE.MinMSIntensity and SE.MinMSMSIntensity to a low value, e.g. 1 2022-09-14 14:07:25,727 INFO [root] # for older Q Exactive data, or when too many MS1 features are extracted, set SE.EstimateBG to yes (or apply SE.MinMSIntensity and SE.MinMSMSIntensity values based on BatMass visualization) 2022-09-14 14:07:25,727 INFO [root] SE.EstimateBG = false 2022-09-14 14:07:25,727 INFO [root] SE.MinMSIntensity = 1 2022-09-14 14:07:25,728 INFO [root] SE.MinMSMSIntensity = 1 2022-09-14 14:07:25,728 INFO [root] 2022-09-14 14:07:25,728 INFO [root] #Signal extraction: peak curve detection and isotope grouping 2022-09-14 14:07:25,729 INFO [root] # for older Q Exactive data, or when too many MS1 features are extracted, set SE.NoMissedScan to 1 2022-09-14 14:07:25,729 INFO [root] SE.NoMissedScan = 2 2022-09-14 14:07:25,729 INFO [root] SE.MaxCurveRTRange = 2 2022-09-14 14:07:25,730 INFO [root] SE.RemoveGroupedPeaks = true 2022-09-14 14:07:25,730 INFO [root] SE.RemoveGroupedPeaksRTOverlap = 0.3 2022-09-14 14:07:25,731 INFO [root] SE.RemoveGroupedPeaksCorr = 0.3 2022-09-14 14:07:25,731 INFO [root] SE.MinNoPeakCluster = 2 2022-09-14 14:07:25,731 INFO [root] SE.MaxNoPeakCluster = 4 2022-09-14 14:07:25,732 INFO [root] 2022-09-14 14:07:25,732 INFO [root] #Signal extraction: filtering of MS1 features 2022-09-14 14:07:25,732 INFO [root] # if interested in modified peptides, increase MassDefectOffset parameter, or set SE.MassDefectFilter to false 2022-09-14 14:07:25,733 INFO [root] SE.IsoPattern = 0.3 2022-09-14 14:07:25,733 INFO [root] SE.MassDefectFilter = true 2022-09-14 14:07:25,734 INFO [root] SE.MassDefectOffset = 0.1 2022-09-14 14:07:25,734 INFO [root] 2022-09-14 14:07:25,735 INFO [root] #Signal extraction: other 2022-09-14 14:07:25,735 INFO [root] SE.StartCharge = 1 2022-09-14 14:07:25,735 INFO [root] SE.EndCharge = 5 2022-09-14 14:07:25,736 INFO [root] SE.MS2StartCharge = 2 2022-09-14 14:07:25,736 INFO [root] SE.MS2EndCharge = 5 2022-09-14 14:07:25,736 INFO [root] SE.MinFrag=10 2022-09-14 14:07:25,736 INFO [root] SE.StartRT = 0 2022-09-14 14:07:25,737 INFO [root] SE.EndRT = 9999 2022-09-14 14:07:25,737 INFO [root] SE.MinMZ = 200 2022-09-14 14:07:25,737 INFO [root] SE.MinPrecursorMass = 600 2022-09-14 14:07:25,738 INFO [root] SE.MaxPrecursorMass = 5000 2022-09-14 14:07:25,738 INFO [root] 2022-09-14 14:07:25,739 INFO [root] 2022-09-14 14:07:25,739 INFO [root] #Isolation window setting 2022-09-14 14:07:25,739 INFO [root] #The current version supports the following window type: SWATH (fixed window size), V_SWATH (variable SWATH window), MSX, MSE, pSMART 2022-09-14 14:07:25,739 INFO [root] WindowType=SWATH 2022-09-14 14:07:25,740 INFO [root] 2022-09-14 14:07:25,740 INFO [root] #Fix window size (For SWATH) 2022-09-14 14:07:25,741 INFO [root] # for Thermo data, this will be determined from raw data automatically 2022-09-14 14:07:25,741 INFO [root] #WindowSize=15 2022-09-14 14:07:25,741 INFO [root] 2022-09-14 14:07:25,742 INFO [root] #Variable SWATH window setting (start m/z, end m/z, separated by Tab) 2022-09-14 14:07:25,742 INFO [root] # for Thermo data, this will be determined from raw data automatically 2022-09-14 14:07:25,742 INFO [root] 2022-09-14 14:07:25,742 INFO [root] #==window setting begin 2022-09-14 14:07:25,743 INFO [root] #==window setting end 2022-09-14 14:07:25,744 INFO [root] ================================================================================================= 2022-09-14 14:07:25,744 INFO [root] Processing /run-files/analysis-20220914-140152/libfree/170825_HF_1ug_DIA_1column_1.mzXML.... 2022-09-14 14:07:25,747 INFO [root] Writing DIA setting to file:/run-files/analysis-20220914-140152/libfree/170825_HF_1ug_DIA_1column_1_diasetting.ser... 2022-09-14 14:07:25,760 INFO [root] Writing parameter to file:/run-files/analysis-20220914-140152/libfree/170825_HF_1ug_DIA_1column_1_params.ser... 2022-09-14 14:07:25,765 INFO [root] Module A: Signal extraction 2022-09-14 14:09:03,303 INFO [root] Writing DIA setting to file:/run-files/analysis-20220914-140152/libfree/170825_HF_1ug_DIA_1column_1_diasetting.ser... 2022-09-14 14:10:12,752 INFO [root] Processing MS1 peak detection 2022-09-14 14:10:12,754 INFO [root] MS1 average cycle time : 3.549224 seconds 2022-09-14 14:10:49,038 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 14:10:49,039 INFO [root] Smoothing detected signals...... 2022-09-14 14:11:50,126 INFO [root] 2626064 Peak curves found (Memory usage:2588MB) 2022-09-14 14:11:50,127 INFO [root] Inclusion mz values found: 0/0 2022-09-14 14:11:50,128 INFO [root] Grouping isotopic peak curves........ 2022-09-14 14:11:50,131 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 14:13:28,330 INFO [root] No of ion clusters:704689 (Memory usage:3184MB) 2022-09-14 14:13:28,554 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_PeakCluster.serFS... 2022-09-14 14:13:41,227 INFO [root] ================================================================================== 2022-09-14 14:13:41,228 INFO [root] Processing DIA MS2 (mz range):985.0_1000.0( 1/40 ) 2022-09-14 14:13:42,169 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 14:13:42,169 INFO [root] Smoothing detected signals...... 2022-09-14 14:14:10,316 INFO [root] 499424 Peak curves found (Memory usage:3572MB) 2022-09-14 14:14:10,320 INFO [root] Grouping isotopic peak curves........ 2022-09-14 14:14:10,320 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 14:14:14,603 INFO [root] No of ion clusters:725 (Memory usage:3643MB) 2022-09-14 14:14:14,609 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_985_1000_PeakCluster.serFS... 2022-09-14 14:14:14,629 INFO [root] Performing mass defect filter on fragment peaks 2022-09-14 14:14:14,629 INFO [root] No. of fragment peaks: 616905 2022-09-14 14:14:14,702 INFO [root] No. of remaining fragment peaks: 591622 2022-09-14 14:14:14,704 INFO [root] Building precursor-fragment pairs for MS1 features.... 2022-09-14 14:14:30,784 INFO [root] Building precursor-fragment pairs for unfragmented ions.... 2022-09-14 14:14:39,192 INFO [root] ================================================================================== 2022-09-14 14:14:39,203 INFO [root] Processing DIA MS2 (mz range):970.0_985.0( 2/40 ) 2022-09-14 14:14:40,081 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 14:14:40,082 INFO [root] Smoothing detected signals...... 2022-09-14 14:15:01,609 INFO [root] 507459 Peak curves found (Memory usage:3231MB) 2022-09-14 14:15:01,612 INFO [root] Grouping isotopic peak curves........ 2022-09-14 14:15:01,612 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 14:15:05,839 INFO [root] No of ion clusters:891 (Memory usage:3303MB) 2022-09-14 14:15:05,840 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_970_985_PeakCluster.serFS... 2022-09-14 14:15:05,852 INFO [root] Performing mass defect filter on fragment peaks 2022-09-14 14:15:05,852 INFO [root] No. of fragment peaks: 618093 2022-09-14 14:15:05,926 INFO [root] No. of remaining fragment peaks: 587579 2022-09-14 14:15:05,927 INFO [root] Building precursor-fragment pairs for MS1 features.... 2022-09-14 14:15:28,357 INFO [root] Building precursor-fragment pairs for unfragmented ions.... 2022-09-14 14:15:37,413 INFO [root] ================================================================================== 2022-09-14 14:15:37,424 INFO [root] Processing DIA MS2 (mz range):955.0_970.0( 3/40 ) 2022-09-14 14:15:38,356 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 14:15:38,357 INFO [root] Smoothing detected signals...... 2022-09-14 14:16:03,511 INFO [root] 517937 Peak curves found (Memory usage:3255MB) 2022-09-14 14:16:03,525 INFO [root] Grouping isotopic peak curves........ 2022-09-14 14:16:03,526 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 14:16:07,749 INFO [root] No of ion clusters:876 (Memory usage:3328MB) 2022-09-14 14:16:07,750 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_955_970_PeakCluster.serFS... 2022-09-14 14:16:07,761 INFO [root] Performing mass defect filter on fragment peaks 2022-09-14 14:16:07,762 INFO [root] No. of fragment peaks: 633211 2022-09-14 14:16:07,817 INFO [root] No. of remaining fragment peaks: 601215 2022-09-14 14:16:07,819 INFO [root] Building precursor-fragment pairs for MS1 features.... 2022-09-14 14:16:35,888 INFO [root] Building precursor-fragment pairs for unfragmented ions.... 2022-09-14 14:16:45,555 INFO [root] ================================================================================== 2022-09-14 14:16:45,566 INFO [root] Processing DIA MS2 (mz range):940.0_955.0( 4/40 ) 2022-09-14 14:16:46,502 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 14:16:46,503 INFO [root] Smoothing detected signals...... 2022-09-14 14:17:11,856 INFO [root] 517853 Peak curves found (Memory usage:3264MB) 2022-09-14 14:17:11,859 INFO [root] Grouping isotopic peak curves........ 2022-09-14 14:17:11,859 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 14:17:16,246 INFO [root] No of ion clusters:948 (Memory usage:3337MB) 2022-09-14 14:17:16,247 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_940_955_PeakCluster.serFS... 2022-09-14 14:17:16,258 INFO [root] Performing mass defect filter on fragment peaks 2022-09-14 14:17:16,259 INFO [root] No. of fragment peaks: 633169 2022-09-14 14:17:16,314 INFO [root] No. of remaining fragment peaks: 602969 2022-09-14 14:17:16,315 INFO [root] Building precursor-fragment pairs for MS1 features.... 2022-09-14 14:17:44,431 INFO [root] Building precursor-fragment pairs for unfragmented ions.... 2022-09-14 14:17:54,178 INFO [root] ================================================================================== 2022-09-14 14:17:54,189 INFO [root] Processing DIA MS2 (mz range):925.0_940.0( 5/40 ) 2022-09-14 14:17:55,127 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 14:17:55,128 INFO [root] Smoothing detected signals...... 2022-09-14 14:18:16,022 INFO [root] 529093 Peak curves found (Memory usage:3288MB) 2022-09-14 14:18:16,027 INFO [root] Grouping isotopic peak curves........ 2022-09-14 14:18:16,027 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 14:18:20,447 INFO [root] No of ion clusters:1004 (Memory usage:3362MB) 2022-09-14 14:18:20,447 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_925_940_PeakCluster.serFS... 2022-09-14 14:18:20,460 INFO [root] Performing mass defect filter on fragment peaks 2022-09-14 14:18:20,461 INFO [root] No. of fragment peaks: 640491 2022-09-14 14:18:20,516 INFO [root] No. of remaining fragment peaks: 608572 2022-09-14 14:18:20,517 INFO [root] Building precursor-fragment pairs for MS1 features.... 2022-09-14 14:18:50,929 INFO [root] Building precursor-fragment pairs for unfragmented ions.... 2022-09-14 14:19:01,252 INFO [root] ================================================================================== 2022-09-14 14:19:01,263 INFO [root] Processing DIA MS2 (mz range):910.0_925.0( 6/40 ) 2022-09-14 14:19:02,211 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 14:19:02,212 INFO [root] Smoothing detected signals...... 2022-09-14 14:19:26,370 INFO [root] 549874 Peak curves found (Memory usage:3324MB) 2022-09-14 14:19:26,375 INFO [root] Grouping isotopic peak curves........ 2022-09-14 14:19:26,376 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 14:19:30,936 INFO [root] No of ion clusters:1078 (Memory usage:3402MB) 2022-09-14 14:19:30,937 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_910_925_PeakCluster.serFS... 2022-09-14 14:19:30,951 INFO [root] Performing mass defect filter on fragment peaks 2022-09-14 14:19:30,951 INFO [root] No. of fragment peaks: 669918 2022-09-14 14:19:31,010 INFO [root] No. of remaining fragment peaks: 638717 2022-09-14 14:19:31,011 INFO [root] Building precursor-fragment pairs for MS1 features.... 2022-09-14 14:20:01,772 INFO [root] Building precursor-fragment pairs for unfragmented ions.... 2022-09-14 14:20:12,668 INFO [root] ================================================================================== 2022-09-14 14:20:12,674 INFO [root] Processing DIA MS2 (mz range):895.0_910.0( 7/40 ) 2022-09-14 14:20:13,628 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 14:20:13,629 INFO [root] Smoothing detected signals...... 2022-09-14 14:20:36,507 INFO [root] 554605 Peak curves found (Memory usage:3349MB) 2022-09-14 14:20:36,512 INFO [root] Grouping isotopic peak curves........ 2022-09-14 14:20:36,512 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 14:20:41,269 INFO [root] No of ion clusters:1034 (Memory usage:3427MB) 2022-09-14 14:20:41,269 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_895_910_PeakCluster.serFS... 2022-09-14 14:20:41,283 INFO [root] Performing mass defect filter on fragment peaks 2022-09-14 14:20:41,283 INFO [root] No. of fragment peaks: 673966 2022-09-14 14:20:41,343 INFO [root] No. of remaining fragment peaks: 644222 2022-09-14 14:20:41,344 INFO [root] Building precursor-fragment pairs for MS1 features.... 2022-09-14 14:21:12,564 INFO [root] Building precursor-fragment pairs for unfragmented ions.... 2022-09-14 14:21:23,404 INFO [root] ================================================================================== 2022-09-14 14:21:23,415 INFO [root] Processing DIA MS2 (mz range):880.0_895.0( 8/40 ) 2022-09-14 14:21:24,339 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 14:21:24,340 INFO [root] Smoothing detected signals...... 2022-09-14 14:21:46,309 INFO [root] 547158 Peak curves found (Memory usage:3369MB) 2022-09-14 14:21:46,311 INFO [root] Grouping isotopic peak curves........ 2022-09-14 14:21:46,312 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 14:21:50,674 INFO [root] No of ion clusters:1045 (Memory usage:3446MB) 2022-09-14 14:21:50,674 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_880_895_PeakCluster.serFS... 2022-09-14 14:21:50,688 INFO [root] Performing mass defect filter on fragment peaks 2022-09-14 14:21:50,688 INFO [root] No. of fragment peaks: 666756 2022-09-14 14:21:50,747 INFO [root] No. of remaining fragment peaks: 628773 2022-09-14 14:21:50,748 INFO [root] Building precursor-fragment pairs for MS1 features.... 2022-09-14 14:22:24,641 INFO [root] Building precursor-fragment pairs for unfragmented ions.... 2022-09-14 14:22:35,395 INFO [root] ================================================================================== 2022-09-14 14:22:35,405 INFO [root] Processing DIA MS2 (mz range):865.0_880.0( 9/40 ) 2022-09-14 14:22:36,399 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 14:22:36,400 INFO [root] Smoothing detected signals...... 2022-09-14 14:22:56,791 INFO [root] 541578 Peak curves found (Memory usage:3393MB) 2022-09-14 14:22:56,793 INFO [root] Grouping isotopic peak curves........ 2022-09-14 14:22:56,794 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 14:23:01,169 INFO [root] No of ion clusters:1007 (Memory usage:3468MB) 2022-09-14 14:23:01,170 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_865_880_PeakCluster.serFS... 2022-09-14 14:23:01,182 INFO [root] Performing mass defect filter on fragment peaks 2022-09-14 14:23:01,182 INFO [root] No. of fragment peaks: 649107 2022-09-14 14:23:01,239 INFO [root] No. of remaining fragment peaks: 623382 2022-09-14 14:23:01,240 INFO [root] Building precursor-fragment pairs for MS1 features.... 2022-09-14 14:23:33,196 INFO [root] Building precursor-fragment pairs for unfragmented ions.... 2022-09-14 14:23:43,286 INFO [root] ================================================================================== 2022-09-14 14:23:43,296 INFO [root] Processing DIA MS2 (mz range):850.0_865.0( 10/40 ) 2022-09-14 14:23:44,187 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 14:23:44,188 INFO [root] Smoothing detected signals...... 2022-09-14 14:24:02,929 INFO [root] 510127 Peak curves found (Memory usage:3378MB) 2022-09-14 14:24:02,931 INFO [root] Grouping isotopic peak curves........ 2022-09-14 14:24:02,932 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 14:24:07,321 INFO [root] No of ion clusters:808 (Memory usage:3449MB) 2022-09-14 14:24:07,322 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_850_865_PeakCluster.serFS... 2022-09-14 14:24:07,333 INFO [root] Performing mass defect filter on fragment peaks 2022-09-14 14:24:07,333 INFO [root] No. of fragment peaks: 610639 2022-09-14 14:24:07,388 INFO [root] No. of remaining fragment peaks: 590351 2022-09-14 14:24:07,389 INFO [root] Building precursor-fragment pairs for MS1 features.... 2022-09-14 14:24:38,253 INFO [root] Building precursor-fragment pairs for unfragmented ions.... 2022-09-14 14:24:47,562 INFO [root] ================================================================================== 2022-09-14 14:24:47,572 INFO [root] Processing DIA MS2 (mz range):835.0_850.0( 11/40 ) 2022-09-14 14:24:48,479 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 14:24:48,479 INFO [root] Smoothing detected signals...... 2022-09-14 14:25:09,401 INFO [root] 558230 Peak curves found (Memory usage:3430MB) 2022-09-14 14:25:09,406 INFO [root] Grouping isotopic peak curves........ 2022-09-14 14:25:09,407 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 14:25:14,122 INFO [root] No of ion clusters:1041 (Memory usage:3507MB) 2022-09-14 14:25:14,123 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_835_850_PeakCluster.serFS... 2022-09-14 14:25:14,136 INFO [root] Performing mass defect filter on fragment peaks 2022-09-14 14:25:14,136 INFO [root] No. of fragment peaks: 668846 2022-09-14 14:25:14,194 INFO [root] No. of remaining fragment peaks: 643816 2022-09-14 14:25:14,195 INFO [root] Building precursor-fragment pairs for MS1 features.... 2022-09-14 14:26:00,489 INFO [root] Building precursor-fragment pairs for unfragmented ions.... 2022-09-14 14:26:12,626 INFO [root] ================================================================================== 2022-09-14 14:26:12,632 INFO [root] Processing DIA MS2 (mz range):820.0_835.0( 12/40 ) 2022-09-14 14:26:13,553 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 14:26:13,554 INFO [root] Smoothing detected signals...... 2022-09-14 14:26:34,927 INFO [root] 535118 Peak curves found (Memory usage:3451MB) 2022-09-14 14:26:34,930 INFO [root] Grouping isotopic peak curves........ 2022-09-14 14:26:34,930 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 14:26:39,527 INFO [root] No of ion clusters:1121 (Memory usage:3525MB) 2022-09-14 14:26:39,527 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_820_835_PeakCluster.serFS... 2022-09-14 14:26:39,543 INFO [root] Performing mass defect filter on fragment peaks 2022-09-14 14:26:39,543 INFO [root] No. of fragment peaks: 641160 2022-09-14 14:26:39,599 INFO [root] No. of remaining fragment peaks: 608138 2022-09-14 14:26:39,600 INFO [root] Building precursor-fragment pairs for MS1 features.... 2022-09-14 14:27:21,332 INFO [root] Building precursor-fragment pairs for unfragmented ions.... 2022-09-14 14:27:32,976 INFO [root] ================================================================================== 2022-09-14 14:27:32,977 INFO [root] Processing DIA MS2 (mz range):805.0_820.0( 13/40 ) 2022-09-14 14:27:33,974 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 14:27:33,975 INFO [root] Smoothing detected signals...... 2022-09-14 14:27:56,004 INFO [root] 591741 Peak curves found (Memory usage:3522MB) 2022-09-14 14:27:56,007 INFO [root] Grouping isotopic peak curves........ 2022-09-14 14:27:56,008 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 14:28:00,515 INFO [root] No of ion clusters:1077 (Memory usage:3603MB) 2022-09-14 14:28:00,515 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_805_820_PeakCluster.serFS... 2022-09-14 14:28:00,529 INFO [root] Performing mass defect filter on fragment peaks 2022-09-14 14:28:00,529 INFO [root] No. of fragment peaks: 709922 2022-09-14 14:28:00,589 INFO [root] No. of remaining fragment peaks: 678905 2022-09-14 14:28:00,590 INFO [root] Building precursor-fragment pairs for MS1 features.... 2022-09-14 14:28:47,873 INFO [root] Building precursor-fragment pairs for unfragmented ions.... 2022-09-14 14:29:00,192 INFO [root] ================================================================================== 2022-09-14 14:29:00,203 INFO [root] Processing DIA MS2 (mz range):790.0_805.0( 14/40 ) 2022-09-14 14:29:00,924 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 14:29:00,924 INFO [root] Smoothing detected signals...... 2022-09-14 14:29:22,678 INFO [root] 578141 Peak curves found (Memory usage:3541MB) 2022-09-14 14:29:22,683 INFO [root] Grouping isotopic peak curves........ 2022-09-14 14:29:22,684 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 14:29:27,723 INFO [root] No of ion clusters:905 (Memory usage:3621MB) 2022-09-14 14:29:27,729 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_790_805_PeakCluster.serFS... 2022-09-14 14:29:27,741 INFO [root] Performing mass defect filter on fragment peaks 2022-09-14 14:29:27,742 INFO [root] No. of fragment peaks: 690389 2022-09-14 14:29:27,802 INFO [root] No. of remaining fragment peaks: 666353 2022-09-14 14:29:27,803 INFO [root] Building precursor-fragment pairs for MS1 features.... 2022-09-14 14:30:13,068 INFO [root] Building precursor-fragment pairs for unfragmented ions.... 2022-09-14 14:30:24,086 INFO [root] ================================================================================== 2022-09-14 14:30:24,093 INFO [root] Processing DIA MS2 (mz range):775.0_790.0( 15/40 ) 2022-09-14 14:30:24,964 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 14:30:24,965 INFO [root] Smoothing detected signals...... 2022-09-14 14:30:49,562 INFO [root] 592944 Peak curves found (Memory usage:3584MB) 2022-09-14 14:30:49,568 INFO [root] Grouping isotopic peak curves........ 2022-09-14 14:30:49,568 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 14:30:54,567 INFO [root] No of ion clusters:1041 (Memory usage:3667MB) 2022-09-14 14:30:54,573 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_775_790_PeakCluster.serFS... 2022-09-14 14:30:54,594 INFO [root] Performing mass defect filter on fragment peaks 2022-09-14 14:30:54,594 INFO [root] No. of fragment peaks: 714282 2022-09-14 14:30:54,658 INFO [root] No. of remaining fragment peaks: 683366 2022-09-14 14:30:54,659 INFO [root] Building precursor-fragment pairs for MS1 features.... 2022-09-14 14:31:40,328 INFO [root] Building precursor-fragment pairs for unfragmented ions.... 2022-09-14 14:31:52,139 INFO [root] ================================================================================== 2022-09-14 14:31:52,145 INFO [root] Processing DIA MS2 (mz range):760.0_775.0( 16/40 ) 2022-09-14 14:31:53,051 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 14:31:53,052 INFO [root] Smoothing detected signals...... 2022-09-14 14:32:16,720 INFO [root] 564207 Peak curves found (Memory usage:3592MB) 2022-09-14 14:32:16,723 INFO [root] Grouping isotopic peak curves........ 2022-09-14 14:32:16,723 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 14:32:21,568 INFO [root] No of ion clusters:942 (Memory usage:3671MB) 2022-09-14 14:32:21,568 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_760_775_PeakCluster.serFS... 2022-09-14 14:32:21,581 INFO [root] Performing mass defect filter on fragment peaks 2022-09-14 14:32:21,581 INFO [root] No. of fragment peaks: 679786 2022-09-14 14:32:21,642 INFO [root] No. of remaining fragment peaks: 625752 2022-09-14 14:32:21,643 INFO [root] Building precursor-fragment pairs for MS1 features.... 2022-09-14 14:33:01,474 INFO [root] Building precursor-fragment pairs for unfragmented ions.... 2022-09-14 14:33:12,354 INFO [root] ================================================================================== 2022-09-14 14:33:12,364 INFO [root] Processing DIA MS2 (mz range):745.0_760.0( 17/40 ) 2022-09-14 14:33:13,234 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 14:33:13,235 INFO [root] Smoothing detected signals...... 2022-09-14 14:33:36,717 INFO [root] 589123 Peak curves found (Memory usage:3644MB) 2022-09-14 14:33:36,719 INFO [root] Grouping isotopic peak curves........ 2022-09-14 14:33:36,720 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 14:33:41,718 INFO [root] No of ion clusters:853 (Memory usage:3725MB) 2022-09-14 14:33:41,724 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_745_760_PeakCluster.serFS... 2022-09-14 14:33:41,734 INFO [root] Performing mass defect filter on fragment peaks 2022-09-14 14:33:41,735 INFO [root] No. of fragment peaks: 706234 2022-09-14 14:33:41,798 INFO [root] No. of remaining fragment peaks: 646830 2022-09-14 14:33:41,799 INFO [root] Building precursor-fragment pairs for MS1 features.... 2022-09-14 14:34:20,448 INFO [root] Building precursor-fragment pairs for unfragmented ions.... 2022-09-14 14:34:30,999 INFO [root] ================================================================================== 2022-09-14 14:34:31,010 INFO [root] Processing DIA MS2 (mz range):730.0_745.0( 18/40 ) 2022-09-14 14:34:31,919 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 14:34:31,919 INFO [root] Smoothing detected signals...... 2022-09-14 14:34:57,507 INFO [root] 554771 Peak curves found (Memory usage:3650MB) 2022-09-14 14:34:57,509 INFO [root] Grouping isotopic peak curves........ 2022-09-14 14:34:57,510 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 14:35:01,853 INFO [root] No of ion clusters:1200 (Memory usage:3728MB) 2022-09-14 14:35:01,853 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_730_745_PeakCluster.serFS... 2022-09-14 14:35:01,868 INFO [root] Performing mass defect filter on fragment peaks 2022-09-14 14:35:01,869 INFO [root] No. of fragment peaks: 670219 2022-09-14 14:35:01,938 INFO [root] No. of remaining fragment peaks: 639340 2022-09-14 14:35:01,938 INFO [root] Building precursor-fragment pairs for MS1 features.... 2022-09-14 14:35:47,963 INFO [root] Building precursor-fragment pairs for unfragmented ions.... 2022-09-14 14:36:00,604 INFO [root] ================================================================================== 2022-09-14 14:36:00,604 INFO [root] Processing DIA MS2 (mz range):715.0_730.0( 19/40 ) 2022-09-14 14:36:01,470 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 14:36:01,470 INFO [root] Smoothing detected signals...... 2022-09-14 14:36:26,689 INFO [root] 617268 Peak curves found (Memory usage:3722MB) 2022-09-14 14:36:26,694 INFO [root] Grouping isotopic peak curves........ 2022-09-14 14:36:26,695 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 14:36:31,100 INFO [root] No of ion clusters:1217 (Memory usage:3807MB) 2022-09-14 14:36:31,100 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_715_730_PeakCluster.serFS... 2022-09-14 14:36:31,117 INFO [root] Performing mass defect filter on fragment peaks 2022-09-14 14:36:31,118 INFO [root] No. of fragment peaks: 738373 2022-09-14 14:36:31,186 INFO [root] No. of remaining fragment peaks: 704740 2022-09-14 14:36:31,186 INFO [root] Building precursor-fragment pairs for MS1 features.... 2022-09-14 14:37:26,490 INFO [root] Building precursor-fragment pairs for unfragmented ions.... 2022-09-14 14:37:40,322 INFO [root] ================================================================================== 2022-09-14 14:37:40,333 INFO [root] Processing DIA MS2 (mz range):700.0_715.0( 20/40 ) 2022-09-14 14:37:41,231 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 14:37:41,232 INFO [root] Smoothing detected signals...... 2022-09-14 14:38:06,987 INFO [root] 620422 Peak curves found (Memory usage:3771MB) 2022-09-14 14:38:06,989 INFO [root] Grouping isotopic peak curves........ 2022-09-14 14:38:06,990 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 14:38:11,722 INFO [root] No of ion clusters:1063 (Memory usage:3857MB) 2022-09-14 14:38:11,722 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_700_715_PeakCluster.serFS... 2022-09-14 14:38:11,743 INFO [root] Performing mass defect filter on fragment peaks 2022-09-14 14:38:11,743 INFO [root] No. of fragment peaks: 744459 2022-09-14 14:38:11,808 INFO [root] No. of remaining fragment peaks: 719674 2022-09-14 14:38:11,809 INFO [root] Building precursor-fragment pairs for MS1 features.... 2022-09-14 14:39:06,343 INFO [root] Building precursor-fragment pairs for unfragmented ions.... 2022-09-14 14:39:19,656 INFO [root] ================================================================================== 2022-09-14 14:39:19,667 INFO [root] Processing DIA MS2 (mz range):685.0_700.0( 21/40 ) 2022-09-14 14:39:20,399 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 14:39:20,399 INFO [root] Smoothing detected signals...... 2022-09-14 14:39:44,475 INFO [root] 524058 Peak curves found (Memory usage:3737MB) 2022-09-14 14:39:44,478 INFO [root] Grouping isotopic peak curves........ 2022-09-14 14:39:44,478 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 14:39:49,192 INFO [root] No of ion clusters:896 (Memory usage:3810MB) 2022-09-14 14:39:49,192 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_685_700_PeakCluster.serFS... 2022-09-14 14:39:49,204 INFO [root] Performing mass defect filter on fragment peaks 2022-09-14 14:39:49,205 INFO [root] No. of fragment peaks: 628290 2022-09-14 14:39:49,267 INFO [root] No. of remaining fragment peaks: 602847 2022-09-14 14:39:49,267 INFO [root] Building precursor-fragment pairs for MS1 features.... 2022-09-14 14:40:31,845 INFO [root] Building precursor-fragment pairs for unfragmented ions.... 2022-09-14 14:40:43,619 INFO [root] ================================================================================== 2022-09-14 14:40:43,620 INFO [root] Processing DIA MS2 (mz range):670.0_685.0( 22/40 ) 2022-09-14 14:40:44,312 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 14:40:44,313 INFO [root] Smoothing detected signals...... 2022-09-14 14:41:07,432 INFO [root] 618747 Peak curves found (Memory usage:3835MB) 2022-09-14 14:41:07,434 INFO [root] Grouping isotopic peak curves........ 2022-09-14 14:41:07,435 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 14:41:12,477 INFO [root] No of ion clusters:1117 (Memory usage:3920MB) 2022-09-14 14:41:12,483 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_670_685_PeakCluster.serFS... 2022-09-14 14:41:12,498 INFO [root] Performing mass defect filter on fragment peaks 2022-09-14 14:41:12,498 INFO [root] No. of fragment peaks: 734218 2022-09-14 14:41:12,576 INFO [root] No. of remaining fragment peaks: 711846 2022-09-14 14:41:12,577 INFO [root] Building precursor-fragment pairs for MS1 features.... 2022-09-14 14:42:03,586 INFO [root] Building precursor-fragment pairs for unfragmented ions.... 2022-09-14 14:42:17,035 INFO [root] ================================================================================== 2022-09-14 14:42:17,046 INFO [root] Processing DIA MS2 (mz range):655.0_670.0( 23/40 ) 2022-09-14 14:42:17,949 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 14:42:17,950 INFO [root] Smoothing detected signals...... 2022-09-14 14:42:43,314 INFO [root] 617782 Peak curves found (Memory usage:3891MB) 2022-09-14 14:42:43,317 INFO [root] Grouping isotopic peak curves........ 2022-09-14 14:42:43,317 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 14:42:48,701 INFO [root] No of ion clusters:1142 (Memory usage:3977MB) 2022-09-14 14:42:48,708 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_655_670_PeakCluster.serFS... 2022-09-14 14:42:48,722 INFO [root] Performing mass defect filter on fragment peaks 2022-09-14 14:42:48,722 INFO [root] No. of fragment peaks: 742649 2022-09-14 14:42:48,788 INFO [root] No. of remaining fragment peaks: 721848 2022-09-14 14:42:48,789 INFO [root] Building precursor-fragment pairs for MS1 features.... 2022-09-14 14:43:36,185 INFO [root] Building precursor-fragment pairs for unfragmented ions.... 2022-09-14 14:43:49,574 INFO [root] ================================================================================== 2022-09-14 14:43:49,584 INFO [root] Processing DIA MS2 (mz range):640.0_655.0( 24/40 ) 2022-09-14 14:43:50,424 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 14:43:50,425 INFO [root] Smoothing detected signals...... 2022-09-14 14:44:11,596 INFO [root] 570270 Peak curves found (Memory usage:3858MB) 2022-09-14 14:44:11,598 INFO [root] Grouping isotopic peak curves........ 2022-09-14 14:44:11,599 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 14:44:16,011 INFO [root] No of ion clusters:1067 (Memory usage:3936MB) 2022-09-14 14:44:16,012 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_640_655_PeakCluster.serFS... 2022-09-14 14:44:16,027 INFO [root] Performing mass defect filter on fragment peaks 2022-09-14 14:44:16,027 INFO [root] No. of fragment peaks: 678383 2022-09-14 14:44:16,088 INFO [root] No. of remaining fragment peaks: 659196 2022-09-14 14:44:16,088 INFO [root] Building precursor-fragment pairs for MS1 features.... 2022-09-14 14:45:03,936 INFO [root] Building precursor-fragment pairs for unfragmented ions.... 2022-09-14 14:45:17,125 INFO [root] ================================================================================== 2022-09-14 14:45:17,136 INFO [root] Processing DIA MS2 (mz range):625.0_640.0( 25/40 ) 2022-09-14 14:45:17,996 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 14:45:17,997 INFO [root] Smoothing detected signals...... 2022-09-14 14:45:39,647 INFO [root] 541789 Peak curves found (Memory usage:3903MB) 2022-09-14 14:45:39,649 INFO [root] Grouping isotopic peak curves........ 2022-09-14 14:45:39,650 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 14:45:44,546 INFO [root] No of ion clusters:952 (Memory usage:3978MB) 2022-09-14 14:45:44,552 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_625_640_PeakCluster.serFS... 2022-09-14 14:45:44,565 INFO [root] Performing mass defect filter on fragment peaks 2022-09-14 14:45:44,565 INFO [root] No. of fragment peaks: 647170 2022-09-14 14:45:44,621 INFO [root] No. of remaining fragment peaks: 628243 2022-09-14 14:45:44,622 INFO [root] Building precursor-fragment pairs for MS1 features.... 2022-09-14 14:46:33,294 INFO [root] Building precursor-fragment pairs for unfragmented ions.... 2022-09-14 14:46:45,881 INFO [root] ================================================================================== 2022-09-14 14:46:45,887 INFO [root] Processing DIA MS2 (mz range):610.0_625.0( 26/40 ) 2022-09-14 14:46:46,718 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 14:46:46,719 INFO [root] Smoothing detected signals...... 2022-09-14 14:47:08,026 INFO [root] 589913 Peak curves found (Memory usage:3969MB) 2022-09-14 14:47:08,028 INFO [root] Grouping isotopic peak curves........ 2022-09-14 14:47:08,029 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 14:47:12,503 INFO [root] No of ion clusters:930 (Memory usage:4049MB) 2022-09-14 14:47:12,503 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_610_625_PeakCluster.serFS... 2022-09-14 14:47:12,514 INFO [root] Performing mass defect filter on fragment peaks 2022-09-14 14:47:12,514 INFO [root] No. of fragment peaks: 692793 2022-09-14 14:47:12,576 INFO [root] No. of remaining fragment peaks: 671334 2022-09-14 14:47:12,577 INFO [root] Building precursor-fragment pairs for MS1 features.... 2022-09-14 14:48:03,297 INFO [root] Building precursor-fragment pairs for unfragmented ions.... 2022-09-14 14:48:16,194 INFO [root] ================================================================================== 2022-09-14 14:48:16,205 INFO [root] Processing DIA MS2 (mz range):595.0_610.0( 27/40 ) 2022-09-14 14:48:17,014 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 14:48:17,014 INFO [root] Smoothing detected signals...... 2022-09-14 14:48:39,137 INFO [root] 579070 Peak curves found (Memory usage:4000MB) 2022-09-14 14:48:39,140 INFO [root] Grouping isotopic peak curves........ 2022-09-14 14:48:39,141 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 14:48:44,043 INFO [root] No of ion clusters:1011 (Memory usage:4078MB) 2022-09-14 14:48:44,048 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_595_610_PeakCluster.serFS... 2022-09-14 14:48:44,061 INFO [root] Performing mass defect filter on fragment peaks 2022-09-14 14:48:44,061 INFO [root] No. of fragment peaks: 681028 2022-09-14 14:48:44,121 INFO [root] No. of remaining fragment peaks: 661482 2022-09-14 14:48:44,121 INFO [root] Building precursor-fragment pairs for MS1 features.... 2022-09-14 14:49:29,467 INFO [root] Building precursor-fragment pairs for unfragmented ions.... 2022-09-14 14:49:41,646 INFO [root] ================================================================================== 2022-09-14 14:49:41,651 INFO [root] Processing DIA MS2 (mz range):580.0_595.0( 28/40 ) 2022-09-14 14:49:42,417 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 14:49:42,418 INFO [root] Smoothing detected signals...... 2022-09-14 14:50:03,130 INFO [root] 549907 Peak curves found (Memory usage:3997MB) 2022-09-14 14:50:03,133 INFO [root] Grouping isotopic peak curves........ 2022-09-14 14:50:03,133 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 14:50:07,892 INFO [root] No of ion clusters:930 (Memory usage:4071MB) 2022-09-14 14:50:07,898 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_580_595_PeakCluster.serFS... 2022-09-14 14:50:07,911 INFO [root] Performing mass defect filter on fragment peaks 2022-09-14 14:50:07,911 INFO [root] No. of fragment peaks: 644275 2022-09-14 14:50:07,976 INFO [root] No. of remaining fragment peaks: 623665 2022-09-14 14:50:07,977 INFO [root] Building precursor-fragment pairs for MS1 features.... 2022-09-14 14:50:49,463 INFO [root] Building precursor-fragment pairs for unfragmented ions.... 2022-09-14 14:51:01,066 INFO [root] ================================================================================== 2022-09-14 14:51:01,066 INFO [root] Processing DIA MS2 (mz range):565.0_580.0( 29/40 ) 2022-09-14 14:51:01,902 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 14:51:01,902 INFO [root] Smoothing detected signals...... 2022-09-14 14:51:22,330 INFO [root] 543289 Peak curves found (Memory usage:4024MB) 2022-09-14 14:51:22,335 INFO [root] Grouping isotopic peak curves........ 2022-09-14 14:51:22,336 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 14:51:26,453 INFO [root] No of ion clusters:1022 (Memory usage:4097MB) 2022-09-14 14:51:26,453 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_565_580_PeakCluster.serFS... 2022-09-14 14:51:26,467 INFO [root] Performing mass defect filter on fragment peaks 2022-09-14 14:51:26,467 INFO [root] No. of fragment peaks: 636011 2022-09-14 14:51:26,523 INFO [root] No. of remaining fragment peaks: 615808 2022-09-14 14:51:26,524 INFO [root] Building precursor-fragment pairs for MS1 features.... 2022-09-14 14:52:11,254 INFO [root] Building precursor-fragment pairs for unfragmented ions.... 2022-09-14 14:52:23,780 INFO [root] ================================================================================== 2022-09-14 14:52:23,791 INFO [root] Processing DIA MS2 (mz range):550.0_565.0( 30/40 ) 2022-09-14 14:52:24,542 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 14:52:24,543 INFO [root] Smoothing detected signals...... 2022-09-14 14:52:43,214 INFO [root] 522119 Peak curves found (Memory usage:4028MB) 2022-09-14 14:52:43,217 INFO [root] Grouping isotopic peak curves........ 2022-09-14 14:52:43,217 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 14:52:47,874 INFO [root] No of ion clusters:980 (Memory usage:4099MB) 2022-09-14 14:52:47,874 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_550_565_PeakCluster.serFS... 2022-09-14 14:52:47,887 INFO [root] Performing mass defect filter on fragment peaks 2022-09-14 14:52:47,887 INFO [root] No. of fragment peaks: 611208 2022-09-14 14:52:47,939 INFO [root] No. of remaining fragment peaks: 593427 2022-09-14 14:52:47,940 INFO [root] Building precursor-fragment pairs for MS1 features.... 2022-09-14 14:53:30,470 INFO [root] Building precursor-fragment pairs for unfragmented ions.... 2022-09-14 14:53:42,253 INFO [root] ================================================================================== 2022-09-14 14:53:42,259 INFO [root] Processing DIA MS2 (mz range):535.0_550.0( 31/40 ) 2022-09-14 14:53:43,102 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 14:53:43,102 INFO [root] Smoothing detected signals...... 2022-09-14 14:54:01,748 INFO [root] 504155 Peak curves found (Memory usage:4059MB) 2022-09-14 14:54:01,752 INFO [root] Grouping isotopic peak curves........ 2022-09-14 14:54:01,753 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 14:54:06,168 INFO [root] No of ion clusters:1135 (Memory usage:4127MB) 2022-09-14 14:54:06,168 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_535_550_PeakCluster.serFS... 2022-09-14 14:54:06,183 INFO [root] Performing mass defect filter on fragment peaks 2022-09-14 14:54:06,183 INFO [root] No. of fragment peaks: 587539 2022-09-14 14:54:06,238 INFO [root] No. of remaining fragment peaks: 564423 2022-09-14 14:54:06,240 INFO [root] Building precursor-fragment pairs for MS1 features.... 2022-09-14 14:54:43,281 INFO [root] Building precursor-fragment pairs for unfragmented ions.... 2022-09-14 14:54:55,384 INFO [root] ================================================================================== 2022-09-14 14:54:55,395 INFO [root] Processing DIA MS2 (mz range):520.0_535.0( 32/40 ) 2022-09-14 14:54:56,265 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 14:54:56,266 INFO [root] Smoothing detected signals...... 2022-09-14 14:55:14,725 INFO [root] 508686 Peak curves found (Memory usage:4097MB) 2022-09-14 14:55:14,728 INFO [root] Grouping isotopic peak curves........ 2022-09-14 14:55:14,728 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 14:55:19,461 INFO [root] No of ion clusters:1058 (Memory usage:4165MB) 2022-09-14 14:55:19,467 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_520_535_PeakCluster.serFS... 2022-09-14 14:55:19,481 INFO [root] Performing mass defect filter on fragment peaks 2022-09-14 14:55:19,481 INFO [root] No. of fragment peaks: 590144 2022-09-14 14:55:19,532 INFO [root] No. of remaining fragment peaks: 570042 2022-09-14 14:55:19,533 INFO [root] Building precursor-fragment pairs for MS1 features.... 2022-09-14 14:55:56,103 INFO [root] Building precursor-fragment pairs for unfragmented ions.... 2022-09-14 14:56:07,439 INFO [root] ================================================================================== 2022-09-14 14:56:07,445 INFO [root] Processing DIA MS2 (mz range):505.0_520.0( 33/40 ) 2022-09-14 14:56:08,309 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 14:56:08,310 INFO [root] Smoothing detected signals...... 2022-09-14 14:56:27,745 INFO [root] 479572 Peak curves found (Memory usage:4109MB) 2022-09-14 14:56:27,747 INFO [root] Grouping isotopic peak curves........ 2022-09-14 14:56:27,747 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 14:56:31,843 INFO [root] No of ion clusters:1033 (Memory usage:4173MB) 2022-09-14 14:56:31,849 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_505_520_PeakCluster.serFS... 2022-09-14 14:56:31,863 INFO [root] Performing mass defect filter on fragment peaks 2022-09-14 14:56:31,864 INFO [root] No. of fragment peaks: 559664 2022-09-14 14:56:31,911 INFO [root] No. of remaining fragment peaks: 536840 2022-09-14 14:56:31,912 INFO [root] Building precursor-fragment pairs for MS1 features.... 2022-09-14 14:57:10,752 INFO [root] Building precursor-fragment pairs for unfragmented ions.... 2022-09-14 14:57:22,023 INFO [root] ================================================================================== 2022-09-14 14:57:22,034 INFO [root] Processing DIA MS2 (mz range):490.0_505.0( 34/40 ) 2022-09-14 14:57:22,945 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 14:57:22,946 INFO [root] Smoothing detected signals...... 2022-09-14 14:57:41,440 INFO [root] 468577 Peak curves found (Memory usage:4139MB) 2022-09-14 14:57:41,443 INFO [root] Grouping isotopic peak curves........ 2022-09-14 14:57:41,443 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 14:57:45,805 INFO [root] No of ion clusters:971 (Memory usage:4202MB) 2022-09-14 14:57:45,806 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_490_505_PeakCluster.serFS... 2022-09-14 14:57:45,818 INFO [root] Performing mass defect filter on fragment peaks 2022-09-14 14:57:45,818 INFO [root] No. of fragment peaks: 551227 2022-09-14 14:57:45,867 INFO [root] No. of remaining fragment peaks: 532638 2022-09-14 14:57:45,868 INFO [root] Building precursor-fragment pairs for MS1 features.... 2022-09-14 14:58:24,754 INFO [root] Building precursor-fragment pairs for unfragmented ions.... 2022-09-14 14:58:36,103 INFO [root] ================================================================================== 2022-09-14 14:58:36,113 INFO [root] Processing DIA MS2 (mz range):475.0_490.0( 35/40 ) 2022-09-14 14:58:36,927 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 14:58:36,928 INFO [root] Smoothing detected signals...... 2022-09-14 14:58:55,679 INFO [root] 427941 Peak curves found (Memory usage:4140MB) 2022-09-14 14:58:55,682 INFO [root] Grouping isotopic peak curves........ 2022-09-14 14:58:55,682 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 14:58:59,889 INFO [root] No of ion clusters:982 (Memory usage:4198MB) 2022-09-14 14:58:59,890 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_475_490_PeakCluster.serFS... 2022-09-14 14:58:59,903 INFO [root] Performing mass defect filter on fragment peaks 2022-09-14 14:58:59,903 INFO [root] No. of fragment peaks: 506889 2022-09-14 14:58:59,948 INFO [root] No. of remaining fragment peaks: 491392 2022-09-14 14:58:59,949 INFO [root] Building precursor-fragment pairs for MS1 features.... 2022-09-14 14:59:33,870 INFO [root] Building precursor-fragment pairs for unfragmented ions.... 2022-09-14 14:59:44,549 INFO [root] ================================================================================== 2022-09-14 14:59:44,560 INFO [root] Processing DIA MS2 (mz range):460.0_475.0( 36/40 ) 2022-09-14 14:59:45,309 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 14:59:45,310 INFO [root] Smoothing detected signals...... 2022-09-14 15:00:03,167 INFO [root] 400786 Peak curves found (Memory usage:4153MB) 2022-09-14 15:00:03,170 INFO [root] Grouping isotopic peak curves........ 2022-09-14 15:00:03,170 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 15:00:07,165 INFO [root] No of ion clusters:846 (Memory usage:4208MB) 2022-09-14 15:00:07,171 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_460_475_PeakCluster.serFS... 2022-09-14 15:00:07,182 INFO [root] Performing mass defect filter on fragment peaks 2022-09-14 15:00:07,182 INFO [root] No. of fragment peaks: 473974 2022-09-14 15:00:07,224 INFO [root] No. of remaining fragment peaks: 455384 2022-09-14 15:00:07,225 INFO [root] Building precursor-fragment pairs for MS1 features.... 2022-09-14 15:00:38,859 INFO [root] Building precursor-fragment pairs for unfragmented ions.... 2022-09-14 15:00:48,699 INFO [root] ================================================================================== 2022-09-14 15:00:48,709 INFO [root] Processing DIA MS2 (mz range):445.0_460.0( 37/40 ) 2022-09-14 15:00:49,476 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 15:00:49,477 INFO [root] Smoothing detected signals...... 2022-09-14 15:01:07,933 INFO [root] 378657 Peak curves found (Memory usage:4146MB) 2022-09-14 15:01:07,935 INFO [root] Grouping isotopic peak curves........ 2022-09-14 15:01:07,936 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 15:01:12,297 INFO [root] No of ion clusters:821 (Memory usage:4197MB) 2022-09-14 15:01:12,298 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_445_460_PeakCluster.serFS... 2022-09-14 15:01:12,315 INFO [root] Performing mass defect filter on fragment peaks 2022-09-14 15:01:12,316 INFO [root] No. of fragment peaks: 447011 2022-09-14 15:01:12,357 INFO [root] No. of remaining fragment peaks: 423254 2022-09-14 15:01:12,357 INFO [root] Building precursor-fragment pairs for MS1 features.... 2022-09-14 15:01:46,354 INFO [root] Building precursor-fragment pairs for unfragmented ions.... 2022-09-14 15:01:56,465 INFO [root] ================================================================================== 2022-09-14 15:01:56,475 INFO [root] Processing DIA MS2 (mz range):430.0_445.0( 38/40 ) 2022-09-14 15:01:57,281 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 15:01:57,282 INFO [root] Smoothing detected signals...... 2022-09-14 15:02:12,600 INFO [root] 379756 Peak curves found (Memory usage:4192MB) 2022-09-14 15:02:12,602 INFO [root] Grouping isotopic peak curves........ 2022-09-14 15:02:12,602 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 15:02:16,899 INFO [root] No of ion clusters:826 (Memory usage:4243MB) 2022-09-14 15:02:16,899 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_430_445_PeakCluster.serFS... 2022-09-14 15:02:16,911 INFO [root] Performing mass defect filter on fragment peaks 2022-09-14 15:02:16,911 INFO [root] No. of fragment peaks: 447248 2022-09-14 15:02:16,952 INFO [root] No. of remaining fragment peaks: 422406 2022-09-14 15:02:16,953 INFO [root] Building precursor-fragment pairs for MS1 features.... 2022-09-14 15:02:50,448 INFO [root] Building precursor-fragment pairs for unfragmented ions.... 2022-09-14 15:03:00,083 INFO [root] ================================================================================== 2022-09-14 15:03:00,097 INFO [root] Processing DIA MS2 (mz range):415.0_430.0( 39/40 ) 2022-09-14 15:03:00,810 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 15:03:00,811 INFO [root] Smoothing detected signals...... 2022-09-14 15:03:19,732 INFO [root] 292324 Peak curves found (Memory usage:4160MB) 2022-09-14 15:03:19,735 INFO [root] Grouping isotopic peak curves........ 2022-09-14 15:03:19,735 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 15:03:23,324 INFO [root] No of ion clusters:654 (Memory usage:4200MB) 2022-09-14 15:03:23,330 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_415_430_PeakCluster.serFS... 2022-09-14 15:03:23,340 INFO [root] Performing mass defect filter on fragment peaks 2022-09-14 15:03:23,340 INFO [root] No. of fragment peaks: 349406 2022-09-14 15:03:23,372 INFO [root] No. of remaining fragment peaks: 331402 2022-09-14 15:03:23,372 INFO [root] Building precursor-fragment pairs for MS1 features.... 2022-09-14 15:03:47,522 INFO [root] Building precursor-fragment pairs for unfragmented ions.... 2022-09-14 15:03:55,329 INFO [root] ================================================================================== 2022-09-14 15:03:55,339 INFO [root] Processing DIA MS2 (mz range):400.0_415.0( 40/40 ) 2022-09-14 15:03:56,207 INFO [root] Processing all scans to detect possible m/z peak curves and 2022-09-14 15:03:56,207 INFO [root] Smoothing detected signals...... 2022-09-14 15:04:14,595 INFO [root] 392972 Peak curves found (Memory usage:4237MB) 2022-09-14 15:04:14,597 INFO [root] Grouping isotopic peak curves........ 2022-09-14 15:04:14,598 INFO [root] Building PeakCurve Mass-RT KD tree 2022-09-14 15:04:19,178 INFO [root] No of ion clusters:1010 (Memory usage:4291MB) 2022-09-14 15:04:19,178 INFO [root] Writing PeakCluster serialization to file:170825_HF_1ug_DIA_1column_1_400_415_PeakCluster.serFS... 2022-09-14 15:04:19,191 INFO [root] Performing mass defect filter on fragment peaks 2022-09-14 15:04:19,191 INFO [root] No. of fragment peaks: 468038 2022-09-14 15:04:19,233 INFO [root] No. of remaining fragment peaks: 439519 2022-09-14 15:04:19,234 INFO [root] Building precursor-fragment pairs for MS1 features.... 2022-09-14 15:04:49,972 INFO [root] Building precursor-fragment pairs for unfragmented ions.... 2022-09-14 15:04:59,973 INFO [root] ================================================================================== 2022-09-14 15:05:00,006 INFO [root] /run-files/analysis-20220914-140152/libfree/170825_HF_1ug_DIA_1column_1.mzXML processed time:0 hour, 57 min, 34 sec 2022-09-14 15:05:00,007 INFO [root] Job complete 2022-09-14 15:05:00,007 INFO [root] ================================================================================================= Running pipeline command: java -Xms97g -Xmx97g -jar /opt/dia-umpire/DIA_Umpire_SE.jar /run-files/analysis-20220914-140152/libfree/170825_HF_1ug_DIA_1column_5.mzXML /run-files/analysis-20220914-140152/diaumpire-params.txt

DIA-Umpire singal extraction analysis (version: v2.1.3) 2022-09-14 15:05:00,717 INFO [root] Version: v2.1.3 2022-09-14 15:05:00,718 INFO [root] Parameter file:/run-files/analysis-20220914-140152/diaumpire-params.txt 2022-09-14 15:05:00,718 INFO [root] Spectra file:/run-files/analysis-20220914-140152/libfree/170825_HF_1ug_DIA_1column_5.mzXML 2022-09-14 15:05:00,721 INFO [root] #Number of threads 2022-09-14 15:05:00,722 INFO [root] # set to the number of cores available 2022-09-14 15:05:00,722 INFO [root] Thread = 5 2022-09-14 15:05:00,723 INFO [root] 2022-09-14 15:05:00,723 INFO [root] #Precursor-fragments grouping parameters 2022-09-14 15:05:00,724 INFO [root] RPmax = 25 2022-09-14 15:05:00,724 INFO [root] RFmax = 300 2022-09-14 15:05:00,724 INFO [root] CorrThreshold = 0.2 2022-09-14 15:05:00,725 INFO [root] DeltaApex = 0.6 2022-09-14 15:05:00,726 INFO [root] RTOverlap = 0.3 2022-09-14 15:05:00,726 INFO [root] 2022-09-14 15:05:00,727 INFO [root] #Fragment intensity adjustments 2022-09-14 15:05:00,727 INFO [root] # change BoostComplementaryIon if later using database search results to build libraries for Skyline/OpenSWATH 2022-09-14 15:05:00,728 INFO [root] AdjustFragIntensity = true 2022-09-14 15:05:00,728 INFO [root] BoostComplementaryIon = true 2022-09-14 15:05:00,729 INFO [root] 2022-09-14 15:05:00,729 INFO [root] #Export detected MS1 features (output feature file can be loaded and mapped to RAW data in BatMass) 2022-09-14 15:05:00,730 INFO [root] ExportPrecursorPeak = false 2022-09-14 15:05:00,730 INFO [root] 2022-09-14 15:05:00,730 INFO [root] #Signal extraction: mass accuracy and resolution 2022-09-14 15:05:00,731 INFO [root] # resolution parameter matters only for data generated in profile mode 2022-09-14 15:05:00,731 INFO [root] SE.MS1PPM = 15 2022-09-14 15:05:00,732 INFO [root] SE.MS2PPM = 25 2022-09-14 15:05:00,732 INFO [root] SE.Resolution = 60000 2022-09-14 15:05:00,733 INFO [root] 2022-09-14 15:05:00,733 INFO [root] #Signal extraction: signal to noise filter 2022-09-14 15:05:00,733 INFO [root] SE.SN = 1.1 2022-09-14 15:05:00,734 INFO [root] SE.MS2SN = 1.1 2022-09-14 15:05:00,734 INFO [root] 2022-09-14 15:05:00,735 INFO [root] #Signal extraction: minimum signal intensity filter 2022-09-14 15:05:00,735 INFO [root] # for Thermo data, filtering is usually not necessary. Set SE.EstimateBG to false and SE.MinMSIntensity and SE.MinMSMSIntensity to a low value, e.g. 1 2022-09-14 15:05:00,736 INFO [root] # for older Q Exactive data, or when too many MS1 features are extracted, set SE.EstimateBG to yes (or apply SE.MinMSIntensity and SE.MinMSMSIntensity values based on BatMass visualization) 2022-09-14 15:05:00,736 INFO [root] SE.EstimateBG = false 2022-09-14 15:05:00,737 INFO [root] SE.MinMSIntensity = 1 2022-09-14 15:05:00,737 INFO [root] SE.MinMSMSIntensity = 1 2022-09-14 15:05:00,737 INFO [root] 2022-09-14 15:05:00,738 INFO [root] #Signal extraction: peak curve detection and isotope

sampie commented 1 year ago

Basically using multiple search engines should increase the number of peptide identification. However, I have noticed that xtandem does really good job and using also comet does not help that much. So, I would say it is fully ok just to use xtandem.

"Warning: Only one FASTA input file was provided, which might not contain contaminants.You probably want to have them!"

If I remember correctly this warning comes from decoy generator component. It is worried if only one fasta file is provided to it. However, all fasta files given by the user have been concatenated to this file by gladiator workflow already, so this warning is non-issue.

andrewyy677 commented 1 year ago

OK, thanks a lot!

Best, Yingying

andrewyy677 commented 1 year ago

OK, my problems are all solved. Thanks for your detailed answers!

Best, Yingying

从 Windows 版邮件https://go.microsoft.com/fwlink/?LinkId=550986发送

发件人: Sami @.> 发送时间: 2022年9月15日 16:11 收件人: @.> 抄送: Yingying SUN @.>; @.> 主题: Re: [elolab/glaDIAtor] glaDIAtor shut down at log: /opt/comet/comet-ms -P/run-files/analysis xxx (Issue #1)

Basically using multiple search engines should increase the number of peptide identification. However, I have noticed that xtandem does really good job and using also comet does not help that much. So, I would say it is fully ok just to use xtandem.

"Warning: Only one FASTA input file was provided, which might not contain contaminants.You probably want to have them!"

If I remember correctly this warning comes from decoy generator component. It is worried if only one fasta file is provided to it. However, all fasta files given by the user have been concatenated to this file by gladiator workflow already, so this warning is non-issue.

— Reply to this email directly, view it on GitHubhttps://apc01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fgithub.com%2Felolab%2FglaDIAtor%2Fissues%2F1%23issuecomment-1247741101&data=05%7C01%7Csunyingying%40westlake.edu.cn%7Cfdb80ff2c6e24cc153c208da96f1ec5a%7C7e82de2f7ef644169b9644c1457be81b%7C1%7C0%7C637988263050358050%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=xl55Qurh5X8zXanXczMD4hlyL6H5isSnESLIGShNKMA%3D&reserved=0, or unsubscribehttps://apc01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fgithub.com%2Fnotifications%2Funsubscribe-auth%2FA2UR3JKNA6JJHM6BHP3H3R3V6LK3XANCNFSM6AAAAAAQA7OWB4&data=05%7C01%7Csunyingying%40westlake.edu.cn%7Cfdb80ff2c6e24cc153c208da96f1ec5a%7C7e82de2f7ef644169b9644c1457be81b%7C1%7C0%7C637988263050358050%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=W17sFJVsRhpLVku7JicJMK3IYOwCAHd9N1qaJv8mwWk%3D&reserved=0. You are receiving this because you authored the thread.Message ID: @.***>

andrewyy677 commented 1 year ago

Sorry for the incomplete snapshot in the last letter, and the new snapshot was attached.

Best, Yingying

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发件人: Yingying SUN @.> 发送时间: 2022年9月13日 19:59 收件人: @.>; @.> 抄送: @.> 主题: 回复: [elolab/glaDIAtor] glaDIAtor shut down at log: /opt/comet/comet-ms -P/run-files/analysis xxx (Issue #1)

Thanks for the quick response! Yeah, there is no error message in the log. I’ve checked the container and it’s still running. The RAM of my computer is 128 GB, which may not be enough for the process. I will give it a try in a larger RAM PC.

Another problem while pulling the glaDIAtor docker was that some parts were always showed “Waiting” and some parts were not 100% downloaded, but it showed “Status: Downloaded newer image for elolab/gladiator:latest” in the end (screenshot attached). I do not know whether it is because the internet speed or something, and whether the pulling is completed, and if it is the reason why the process stopped after the comet run analysis.

Thanks again!

Best, Yingying

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发件人: Sami @.> 发送时间: 2022年9月13日 16:28 收件人: @.> 抄送: Yingying SUN @.>; @.> 主题: Re: [elolab/glaDIAtor] glaDIAtor shut down at log: /opt/comet/comet-ms -P/run-files/analysis xxx (Issue #1)

Hi

Indeed, it looks like there is nothing in the log after the comet run analysis was started. Not even an error message. It looks like the container might have been stopped for some reason. Can you check if the container is still running? One possible explanation could be that system has run out of memory and process / container has been killed.

— Reply to this email directly, view it on GitHubhttps://apc01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fgithub.com%2Felolab%2FglaDIAtor%2Fissues%2F1%23issuecomment-1245073059&data=05%7C01%7Csunyingying%40westlake.edu.cn%7Cbfd4a02f817a4861a07108da9561f9d6%7C7e82de2f7ef644169b9644c1457be81b%7C1%7C0%7C637986545290734214%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=%2FrzZ%2Bjlq0ac7quvrnB%2F1WN5S%2FkAz4xVOnBSCWxJSR%2Fg%3D&reserved=0, or unsubscribehttps://apc01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fgithub.com%2Fnotifications%2Funsubscribe-auth%2FA2UR3JOWXXKEUVJO33GXW7DV6A3LXANCNFSM6AAAAAAQA7OWB4&data=05%7C01%7Csunyingying%40westlake.edu.cn%7Cbfd4a02f817a4861a07108da9561f9d6%7C7e82de2f7ef644169b9644c1457be81b%7C1%7C0%7C637986545290734214%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=Ej358bng0UIPpNsXoON9svMpQWkdrcPZxH6nuTe8%2B%2FQ%3D&reserved=0. You are receiving this because you authored the thread.Message ID: @.***>

andrewyy677 commented 1 year ago

Thanks for the quick response! Yeah, there is no error message in the log. I’ve checked the container and it’s still running. The RAM of my computer is 128 GB, which may not be enough for the process. I will give it a try in a larger RAM PC.

Another problem while pulling the glaDIAtor docker was that some parts were always showed “Waiting” and some parts were not 100% downloaded, but it showed “Status: Downloaded newer image for elolab/gladiator:latest” in the end (screenshot attached). I do not know whether it is because the internet speed or something, and whether the pulling is completed, and if it is the reason why the process stopped after the comet run analysis.

Thanks again!

Best, Yingying

从 Windows 版邮件https://go.microsoft.com/fwlink/?LinkId=550986发送

发件人: Sami @.> 发送时间: 2022年9月13日 16:28 收件人: @.> 抄送: Yingying SUN @.>; @.> 主题: Re: [elolab/glaDIAtor] glaDIAtor shut down at log: /opt/comet/comet-ms -P/run-files/analysis xxx (Issue #1)

Hi

Indeed, it looks like there is nothing in the log after the comet run analysis was started. Not even an error message. It looks like the container might have been stopped for some reason. Can you check if the container is still running? One possible explanation could be that system has run out of memory and process / container has been killed.

— Reply to this email directly, view it on GitHubhttps://apc01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fgithub.com%2Felolab%2FglaDIAtor%2Fissues%2F1%23issuecomment-1245073059&data=05%7C01%7Csunyingying%40westlake.edu.cn%7Cbfd4a02f817a4861a07108da9561f9d6%7C7e82de2f7ef644169b9644c1457be81b%7C1%7C0%7C637986545290734214%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=%2FrzZ%2Bjlq0ac7quvrnB%2F1WN5S%2FkAz4xVOnBSCWxJSR%2Fg%3D&reserved=0, or unsubscribehttps://apc01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fgithub.com%2Fnotifications%2Funsubscribe-auth%2FA2UR3JOWXXKEUVJO33GXW7DV6A3LXANCNFSM6AAAAAAQA7OWB4&data=05%7C01%7Csunyingying%40westlake.edu.cn%7Cbfd4a02f817a4861a07108da9561f9d6%7C7e82de2f7ef644169b9644c1457be81b%7C1%7C0%7C637986545290734214%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=Ej358bng0UIPpNsXoON9svMpQWkdrcPZxH6nuTe8%2B%2FQ%3D&reserved=0. You are receiving this because you authored the thread.Message ID: @.***>

andrewyy677 commented 1 year ago

Hi, elolab team

  Thanks for previous answers! I tested glaDIAtor using example data (DDA rawdata: 170825_HF_1_6_pool_2ug_DDA_library_1column_1.raw; DIA rawdata: 170825_HF_1ug_DIA_1column_1.raw) (Software GUI attached). I got error message: “AttributeError: 'Spectra' object has no attribute 'name'” at the end of the log.txt (attached). How can I solve the problem?
   Thanks for your time!

Best, Yingying

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发件人: Yingying SUN @.> 发送时间: 2022年9月15日 16:40 收件人: @.>; @.> 抄送: @.> 主题: 回复: [elolab/glaDIAtor] glaDIAtor shut down at log: /opt/comet/comet-ms -P/run-files/analysis xxx (Issue #1)

OK, my problems are all solved. Thanks for your detailed answers!

Best, Yingying

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发件人: Sami @.> 发送时间: 2022年9月15日 16:11 收件人: @.> 抄送: Yingying SUN @.>; @.> 主题: Re: [elolab/glaDIAtor] glaDIAtor shut down at log: /opt/comet/comet-ms -P/run-files/analysis xxx (Issue #1)

Basically using multiple search engines should increase the number of peptide identification. However, I have noticed that xtandem does really good job and using also comet does not help that much. So, I would say it is fully ok just to use xtandem.

"Warning: Only one FASTA input file was provided, which might not contain contaminants.You probably want to have them!"

If I remember correctly this warning comes from decoy generator component. It is worried if only one fasta file is provided to it. However, all fasta files given by the user have been concatenated to this file by gladiator workflow already, so this warning is non-issue.

— Reply to this email directly, view it on GitHubhttps://apc01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fgithub.com%2Felolab%2FglaDIAtor%2Fissues%2F1%23issuecomment-1247741101&data=05%7C01%7Csunyingying%40westlake.edu.cn%7Cfdb80ff2c6e24cc153c208da96f1ec5a%7C7e82de2f7ef644169b9644c1457be81b%7C1%7C0%7C637988263050358050%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=xl55Qurh5X8zXanXczMD4hlyL6H5isSnESLIGShNKMA%3D&reserved=0, or unsubscribehttps://apc01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fgithub.com%2Fnotifications%2Funsubscribe-auth%2FA2UR3JKNA6JJHM6BHP3H3R3V6LK3XANCNFSM6AAAAAAQA7OWB4&data=05%7C01%7Csunyingying%40westlake.edu.cn%7Cfdb80ff2c6e24cc153c208da96f1ec5a%7C7e82de2f7ef644169b9644c1457be81b%7C1%7C0%7C637988263050358050%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=W17sFJVsRhpLVku7JicJMK3IYOwCAHd9N1qaJv8mwWk%3D&reserved=0. You are receiving this because you authored the thread.Message ID: @.***>

Running pipeline command: mono /opt/ThermoRawFileParser/ThermoRawFileParser.exe -f=1 -m=0 --noZlibCompression -i=/data/DIA_raw_example/raw/170825_HF_1ug_DIA_1column_1.raw -b=/run-files/example_fecal_2nd/DIA/170825_HF_1ug_DIA_1column_1.mzML -p 2022-10-31 01:30:46 INFO Started parsing /data/DIA_raw_example/raw/170825_HF_1ug_DIA_1column_1.raw 2022-10-31 01:38:37 INFO Processing 138621 MS scans 10% 20% 30% 40% 50% 60% 70% 80% 90% 100%

2022-10-31 02:38:13 INFO Finished parsing /data/DIA_raw_example/raw/170825_HF_1ug_DIA_1column_1.raw Running pipeline command: mono /opt/ThermoRawFileParser/ThermoRawFileParser.exe -f=1 -m=0 --noZlibCompression -i=/data/DDA_raw_example/raw/170825_HF_1_6_pool_2ug_DDA_library_1column_1.raw -b=/run-files/example_fecal_2nd/DDA/170825_HF_1_6_pool_2ug_DDA_library_1column_1.mzML 2022-10-31 02:38:14 INFO Started parsing /data/DDA_raw_example/raw/170825_HF_1_6_pool_2ug_DDA_library_1column_1.raw 2022-10-31 02:41:34 INFO Processing 123730 MS scans 10% 20% 30% 40% 50% 60% 70% 80% 90% 100%

2022-10-31 02:48:44 INFO Finished parsing /data/DDA_raw_example/raw/170825_HF_1_6_pool_2ug_DDA_library_1column_1.raw Running pipeline command: DecoyDatabase -in DB.fasta -out DB_with_decoys.fasta Warning: Only one FASTA input file was provided, which might not contain contaminants.You probably want to have them! Just add the contaminant file to the input file list 'in'. DecoyDatabase took 18:32 m (wall), 12:58 m (CPU), 13.16 s (system), 12:45 m (user). Running pipeline command: /opt/tandem/tandem /run-files/example_fecal_2nd/tmpvt6259mz 170825_HF_1_6_pool_2ug_DDA_library_1column_1.TANDEM.OUTPUT.xml

X! TANDEM Alanine (2017.2.1.4)

Loading spectra (mzML)..................................................... loaded. Spectra matching criteria = 104654 Starting threads ........................................ started. Computing models: testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 50 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 100 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3test | 150 ks ing 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3t | 200 ks esting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 250 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 300 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3testi | 350 ks ng 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3te | 400 ks sting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 450 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 500 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testin | 550 ks g 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3tes | 600 ks ting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3 | 650 ks testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 700 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 750 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3test | 800 ks ing 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3t | 850 ks esting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 900 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 950 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3testi | 1000 ks ng 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3te | 1050 ks sting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 1100 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 1150 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testin | 1200 ks g 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3tes | 1250 ks ting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3 | 1300 ks testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 1350 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 1400 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3test | 1450 ks ing 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3t | 1500 ks esting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 1550 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 1600 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3testi | 1650 ks ng 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3te | 1700 ks sting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 1750 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 1800 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testin | 1850 ks g 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3tes | 1900 ks ting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3 | 1950 ks testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 2000 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 2050 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3test | 2100 ks ing 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3t | 2150 ks esting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 2200 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 2250 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3testi | 2300 ks ng 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3te | 2350 ks sting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 2400 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 2450 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testin | 2500 ks g 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3tes | 2550 ks ting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3 | 2600 ks testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 2650 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 2700 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3test | 2750 ks ing 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3t | 2800 ks esting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 2850 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 2900 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3testi | 2950 ks ng 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3te | 3000 ks sting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 3050 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 3100 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testin | 3150 ks g 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3tes | 3200 ks ting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3 | 3250 ks testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 3300 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 3350 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3test | 3400 ks ing 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3t | 3450 ks esting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 3500 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 3550 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3testi | 3600 ks ng 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3te | 3650 ks sting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 3700 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 3750 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testin | 3800 ks g 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3tes | 3850 ks ting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3 | 3900 ks testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 3950 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 4000 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3test | 4050 ks ing 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3t | 4100 ks esting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 4150 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 4200 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3testi | 4250 ks ng 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3te | 4300 ks sting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 4350 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 4400 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testin | 4450 ks g 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3tes | 4500 ks ting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3 | 4550 ks testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 4600 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 4650 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3test | 4700 ks ing 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3t | 4750 ks esting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 4800 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 4850 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3testi | 4900 ks ng 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3te | 4950 ks sting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 5000 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 5050 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testin | 5100 ks g 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3tes | 5150 ks ting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3 | 5200 ks testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 5250 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 5300 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3test | 5350 ks ing 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3t | 5400 ks esting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 5450 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 5500 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3testi | 5550 ks ng 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3te | 5600 ks sting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 5650 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 5700 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testin | 5750 ks g 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3tes | 5800 ks ting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3 | 5850 ks testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 5900 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 5950 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3test | 6000 ks ing 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3t | 6050 ks esting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 6100 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 6150 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3testi | 6200 ks ng 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3te | 6250 ks sting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 6300 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 6350 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testin | 6400 ks g 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3tes | 6450 ks ting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3 | 6500 ks testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 6550 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 6600 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3test | 6650 ks ing 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3t | 6700 ks esting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 6750 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 6800 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3testi | 6850 ks ng 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3te | 6900 ks sting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 6950 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 7000 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testin | 7050 ks g 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3tes | 7100 ks ting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3 | 7150 ks testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 7200 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 7250 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3test | 7300 ks ing 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3t | 7350 ks esting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 7400 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 7450 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3testi | 7500 ks ng 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3te | 7550 ks sting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 7600 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 7650 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testin | 7700 ks g 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3tes | 7750 ks ting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3 | 7800 ks testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 7850 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 7900 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3test | 7950 ks ing 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3t | 8000 ks esting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 8050 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 8100 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3testi | 8150 ks ng 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3te | 8200 ks sting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 8250 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 8300 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testin | 8350 ks g 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3tes | 8400 ks ting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3 | 8450 ks testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 8500 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 8550 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3test | 8600 ks ing 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3t | 8650 ks esting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 8700 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 8750 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3testi | 8800 ks ng 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3te | 8850 ks sting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 8900 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 8950 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testin | 9000 ks g 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3tes | 9050 ks ting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3 | 9100 ks testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 9150 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 9200 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3test | 9250 ks ing 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3t | 9300 ks esting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 9350 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 9400 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3testi | 9450 ks ng 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3te | 9500 ks sting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 9550 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 9600 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testin | 9650 ks g 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3tes | 9700 ks ting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3 | 9750 ks testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 9800 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 9850 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3test | 9900 ks ing 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3t | 9950 ks esting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 10000 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 10050 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3testi | 10100 ks ng 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3te | 10150 ks sting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 10200 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 10250 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testin | 10300 ks g 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3tes | 10350 ks ting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3 | 10400 ks testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 10450 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 10500 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3test | 10550 ks ing 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3t | 10600 ks esting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 10650 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 10700 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3testi | 10750 ks ng 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3te | 10800 ks sting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 10850 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 10900 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testin | 10950 ks g 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3tes | 11000 ks ting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3 | 11050 ks testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 11100 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 11150 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3test | 11200 ks ing 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3t | 11250 ks esting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 11300 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 11350 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3testi | 11400 ks ng 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3te | 11450 ks sting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 11500 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 11550 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testin | 11600 ks g 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3tes | 11650 ks ting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3 | 11700 ks testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 11750 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 11800 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3test | 11850 ks ing 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3t | 11900 ks esting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 11950 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 12000 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3testi | 12050 ks ng 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3te | 12100 ks sting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 12150 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 12200 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testin | 12250 ks g 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3tes | 12300 ks ting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3 | 12350 ks testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 12400 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 12450 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3test | 12500 ks ing 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3t | 12550 ks esting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 12600 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 12650 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3testi | 12700 ks ng 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3te | 12750 ks sting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 12800 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 12850 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testin | 12900 ks g 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3tes | 12950 ks ting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3 | 13000 ks testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 13050 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 13100 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3test | 13150 ks ing 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3t | 13200 ks esting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 13250 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 13300 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3testi | 13350 ks ng 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3te | 13400 ks sting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 13450 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 13500 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testin | 13550 ks g 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3tes | 13600 ks ting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3 | 13650 ks testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 13700 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 13750 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3test | 13800 ks ing 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3t | 13850 ks esting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 13900 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 13950 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3testi | 14000 ks ng 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3te | 14050 ks sting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 14100 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 14150 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testin | 14200 ks g 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3tes | 14250 ks ting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3 | 14300 ks testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 14350 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 14400 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3test | 14450 ks ing 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3t | 14500 ks esting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 14550 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 14600 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3testi | 14650 ks ng 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3te | 14700 ks sting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 14750 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 14800 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testin | 14850 ks g 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3tes | 14900 ks ting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3 | 14950 ks testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 15000 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 15050 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3test | 15100 ks ing 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3t | 15150 ks esting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 15200 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 15250 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3testi | 15300 ks ng 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3te | 15350 ks sting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 15400 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 15450 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testin | 15500 ks g 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3tes | 15550 ks ting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3 | 15600 ks testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 15650 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 15700 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3test | 15750 ks ing 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3t | 15800 ks esting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 15850 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 15900 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3testi | 15950 ks ng 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3te | 16000 ks sting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 16050 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 16100 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testin | 16150 ks g 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3tes | 16200 ks ting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3 | 16250 ks testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 16300 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 16350 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3test | 16400 ks ing 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3t | 16450 ks esting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 16500 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 16550 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3testi | 16600 ks ng 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3te | 16650 ks sting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 16700 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 16750 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testin | 16800 ks g 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3tes | 16850 ks ting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3 | 16900 ks testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 16950 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 17000 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3test | 17050 ks ing 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3t | 17100 ks esting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 17150 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 17200 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3testi | 17250 ks ng 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3te | 17300 ks sting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 17350 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 17400 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testin | 17450 ks g 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3tes | 17500 ks ting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3 | 17550 ks testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 17600 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 17650 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3test | 17700 ks ing 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3t | 17750 ks esting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 17800 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 17850 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3testi | 17900 ks ng 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3te | 17950 ks sting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 18000 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 18050 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testin | 18100 ks g 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3tes | 18150 ks ting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3 | 18200 ks testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 18250 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 18300 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3test | 18350 ks ing 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3t | 18400 ks esting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 18450 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 18500 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3testi | 18550 ks ng 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3te | 18600 ks sting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 18650 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 18700 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testin | 18750 ks g 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3tes | 18800 ks ting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3 | 18850 ks testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 18900 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 18950 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3test | 19000 ks ing 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3t | 19050 ks esting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 19100 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 19150 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3testi | 19200 ks ng 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3te | 19250 ks sting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 19300 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 19350 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testin | 19400 ks g 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3tes | 19450 ks ting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3 | 19500 ks testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 19550 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 19600 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3test | 19650 ks ing 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3t | 19700 ks esting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 19750 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 19800 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3testi | 19850 ks ng 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3te | 19900 ks sting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 19950 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 20000 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testin | 20050 ks g 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3tes | 20100 ks ting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3 | 20150 ks testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 20200 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 20250 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3test | 20300 ks ing 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3t | 20350 ks esting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 20400 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing | 20450 ks 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3testi | 20500 ks ng 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3te | 20550 ks sting 1 2 3testing 1 2 3testing 1 2 3testing 1 2 | 20600 ks 3testing 1 2 3testing 1 2 3testing 1 2 3testing 1 | 20650 ks 2 3testing 1 2 3testing 1 2 3testing 1 2 3testin | 20700 ks g 1 2 3testing 1 2 3testing 1 2 3testing 1 2 3 sequences modelled = 20745 ks Model refinement: partial cleavage ..... done. unanticipated cleavage ..... done. modified N-terminus 42.0106@[ ..... done. finishing refinement ... done. Merging results: from 2..3..4..5..6..7..8..9..10..11..12..13..14..15..16..17..18..19..20..21..22..23..24..25..26..27..28..29..30..31..32..33..34..35..36..37..38..39..40..

Creating report: initial calculations ..... done. sorting ..... done. finding repeats ..... done. evaluating results ..... done. calculating expectations ..... done. writing results ..... done.

Valid models = 24348 Unique models = 19946 Estimated false positives = 220 ± 15

Running pipeline command: /opt/tpp/bin/Tandem2XML 170825_HF_1_6_pool_2ug_DDA_library_1column_1.TANDEM.OUTPUT.xml 170825_HF_1_6_pool_2ug_DDA_library_1column1.tandem.pep.xml No index list offset found. File will not be read. Incomplete file name. No file loaded: Incomplete file name. No file loaded: Incomplete file name. No file loaded: Incomplete file name. No file loaded: WARNING: Failed to open mzML file. Output will not contain retention times. Running pipeline command: /opt/tpp/bin/xinteract -OARPd -dDECOY -Ninteract_xtandem_pep.xml 170825_HF_1_6_pool_2ug_DDA_library_1column_1.tandem.pep.xml

/opt/tpp/bin/xinteract (TPP v5.2.0 Flammagenitus, Build 202104252123-8446 (Linux-x86_64))

running: "/opt/tpp/bin/InteractParser 'interact_xtandem_pep.xml' '170825_HF_1_6_pool_2ug_DDA_library_1column_1.tandem.pep.xml' -L'7'" file 1: 170825_HF_1_6_pool_2ug_DDA_library_1column_1.tandem.pep.xml WARNING: cannot open data file /run-files/example_fecal_2nd/170825_HF_1_6_pool_2ug_DDA_library_1column_1.TANDEM.O.mzML in msms_run_summary tag... trying .mzXML ... WARNING: CANNOT correct data file /run-files/example_fecal_2nd/170825_HF_1_6_pool_2ug_DDA_library_1column_1.TANDEM.O.mzXML in msms_run_summary tag... WARNING: cannot open data file /run-files/example_fecal_2nd/170825_HF_1_6_pool_2ug_DDA_library_1column_1.TANDEM.O.mzML in msms_run_summary tag... trying .mzXML ... WARNING: CANNOT correct data file /run-files/example_fecal_2nd/170825_HF_1_6_pool_2ug_DDA_library_1column_1.TANDEM.O.mzXML in msms_run_summary tag... processed altogether 24348 results INFO: Results written to file: /run-files/example_fecal_2nd/interact_xtandem_pep.xml command completed in 483 sec

running: "/opt/tpp/bin/DatabaseParser 'interact_xtandem_pep.xml'" command completed in 0 sec

running: "/opt/tpp/bin/RefreshParser 'interact_xtandem_pep.xml' 'DB_with_decoys.fasta'"

command completed in 5208 sec

running: "/opt/tpp/bin/PeptideProphetParser 'interact_xtandempep.xml' ACCMASS RT NONPARAM DECOYPROBS DECOY=DECOY" using Accurate Mass Bins using RT Using Decoy Label "DECOY_". Decoy Probabilities will be reported. Using non-parametric distributions (X! Tandem) WARNING: Found unanticipated cleavage in refinement mode. Turning off NTT model. adding ACCMASS mixture distribution adding Retention Time mixture distr init with X! Tandem trypsin MS Instrument info: Manufacturer: UNKNOWN, Model: UNKNOWN, Ionization: UNKNOWN, Analyzer: UNKNOWN, Detector: UNKNOWN

INFO: Processing standard MixtureModel ... PeptideProphet (TPP v5.2.0 Flammagenitus, Build 202104252123-8446 (Linux-x86_64)) @.*** read in 0 1+, 16142 2+, 7206 3+, 985 4+, 0 5+, 0 6+, and 0 7+ spectra. Initialising statistical models ... Found 618 Decoys, and 23715 Non-Decoys Iterations: .........10.........20.....Estimating Retention Time Model ... please wait ... Run Index: /run-files/example_fecal_2nd/170825_HF_1_6_pool_2ug_DDA_library_1column_1.TANDEM.O, slope=0.846514, intercept=420.554, r_sq=0.878371 Run Index: /run-files/example_fecal_2nd/170825_HF_1_6_pool_2ug_DDA_library_1column_1.TANDEM.O, slope=0.846514, intercept=420.554, r_sq=0.878371

WARNING: Mixture model quality test failed for charge (1+). WARNING: Mixture model quality test failed for charge (5+). WARNING: Mixture model quality test failed for charge (6+). WARNING: Mixture model quality test failed for charge (7+). model complete after 26 iterations command completed in 337 sec

running: "/opt/tpp/bin/ProphetModels.pl -i interact_xtandempep.xml -d "DECOY"" Analyzing interact_xtandem_pep.xml ... Reading Accurate Mass Model model +1 ... Reading kernel density RTCalc RT [RT] model +1 ... Reading Accurate Mass Model model +2 ... Reading kernel density RTCalc RT [RT] model +2 ... Reading Accurate Mass Model model +3 ... Reading kernel density RTCalc RT [RT] model +3 ... Reading Accurate Mass Model model +4 ... Reading kernel density RTCalc RT [RT] model +4 ... Reading Accurate Mass Model model +5 ... Reading kernel density RTCalc RT [RT] model +5 ... Reading Accurate Mass Model model +6 ... Reading kernel density RTCalc RT [RT] model +6 ... Reading Accurate Mass Model model +7 ... Reading kernel density RTCalc RT [RT] model +7 ... Parsing search results "/run-files/example_fecal_2nd/170825_HF_1_6_pool_2ug_DDA_library_1column_1.TANDEM.O (X! Tandem)"... => Found 20332 hits. (104 decoys, 0 excluded) => Total so far: 20332 hits. (104 decoys, 0 excluded) command completed in 7 sec

running: "/opt/tpp/cgi-bin/PepXMLViewer.cgi -I /run-files/example_fecal_2nd/interact_xtandem_pep.xml" command completed in 9 sec

running: "/opt/tpp/bin/tpp_models.pl '/run-files/example_fecal_2nd/interact_xtandem_pep.xml'" File: /run-files/example_fecal_2nd/interact_xtandem_pep.xml

command completed in 7 sec /opt/tpp/bin/InteractParser 'interact_xtandem_pep.xml' '170825_HF_1_6_pool_2ug_DDA_library_1column_1.tandem.pep.xml' -L'7' 483 sec /opt/tpp/bin/DatabaseParser 'interact_xtandem_pep.xml' /opt/tpp/bin/RefreshParser 'interact_xtandem_pep.xml' 'DB_with_decoys.fasta' 5208 sec /opt/tpp/bin/PeptideProphetParser 'interact_xtandempep.xml' ACCMASS RT NONPARAM DECOYPROBS DECOY=DECOY 337 sec /opt/tpp/bin/ProphetModels.pl -i interact_xtandempep.xml -d "DECOY" 7 sec /opt/tpp/cgi-bin/PepXMLViewer.cgi -I /run-files/example_fecal_2nd/interact_xtandem_pep.xml 9 sec /opt/tpp/bin/tpp_models.pl '/run-files/example_fecal_2nd/interact_xtandem_pep.xml' 7 sec job completed in 6051 sec Running pipeline command: /opt/tpp/bin/Mayu.pl -A ../interact_xtandem_pep.xml -C ../DB_withdecoys.fasta -E DECOY -G 0.01 -H 51 -I 2 -P protFDR=0.01:t

/opt/tpp/bin/Mayu.pl 1.07

6103 seconds run time

Running pipeline command: cat _psm_protFDR0.csv |cut -f 5 -d ',' |tail -n+2 |sort -u |head -n1 Running pipeline command: /opt/tpp/bin/spectrast -cNSpecLiblib -cIHCD -cf "Protein! ~ DECOY" -cP0.3644 -c_IRT/opt/gladiator/iRT.txt -c_IRR interact_xtandem_pep.xml SpectraST started at Mon Oct 31 14:17:35 2022. Processing "interact_xtandem_pep.xml"...500...1000...1500...2000...2500...3000...3500...4000...4500...5000...5500...6000...6500...7000...7500...8000...8500...9000...9500...10000...10500...11000...11500...12000...12500...13000...13500...14000...14500...15000...15500...16000...16500...17000...17500...18000...18500...19000...19500...20000...DONE! Importing all spectra with P>=0.3644 ...10%...20%...30%...40%...50%...60%...70%...80%...90%...DONE!

Library file (BINARY) "SpecLib_lib.splib" created. Library file (TEXT) "SpecLib_lib.sptxt" created. M/Z Index file "SpecLib_lib.spidx" created. Peptide Index file "SpecLib_lib.pepidx" created.

Total number of spectra in library: 0 Total number of distinct peptide ions in library: 0 Total number of distinct stripped peptides in library: 0

CHARGE +1: 0 ; +2: 0 ; +3: 0 ; +4: 0 ; +5: 0 ; >+5: 0 ; Unk: 0 TERMINI Tryptic: 0 ; Semi-tryptic: 0 ; Non-tryptic: 0 PROBABILITY >0.9999: 0 ; 0.999-0.9999: 0 ; 0.99-0.999: 0 ; 0.9-0.99: 0 ; <0.9: 0 NREPS 20+: 0 ; 10-19: 0 ; 4-9: 0 ; 2-3: 0 ; 1: 0 MODIFICATIONS None

Total Run Time = 19 seconds. SpectraST finished at Mon Oct 31 14:17:54 2022 with 1 error(s): PEPXML IMPORT: Cannot open file "170825_HF_1_6_pool_2ug_DDA_library_1column_1.mzXML|.mzML|.mzXML.gz|.mzML.gz|.mz5 in /run-files/example_fecal_2nd/". No scan from this file will be imported.

Running pipeline command: /opt/tpp/bin/spectrast -cNSpecLib_cons -cDDB_with_decoys.fasta -cIHCD -cAC SpecLib_lib.splib SpectraST started at Mon Oct 31 14:17:54 2022. Creating CONSENSUS library from "/run-files/example_fecal_2nd/SpecLib_lib.splib" REFRESHING protein mappings...DONE! Importing ions...DONE!

Library file (BINARY) "SpecLib_cons.splib" created. Library file (TEXT) "SpecLib_cons.sptxt" created. M/Z Index file "SpecLib_cons.spidx" created. Peptide Index file "SpecLib_cons.pepidx" created.

Total number of spectra in library: 0 Total number of distinct peptide ions in library: 0 Total number of distinct stripped peptides in library: 0

CHARGE +1: 0 ; +2: 0 ; +3: 0 ; +4: 0 ; +5: 0 ; >+5: 0 ; Unk: 0 TERMINI Tryptic: 0 ; Semi-tryptic: 0 ; Non-tryptic: 0 PROBABILITY >0.9999: 0 ; 0.999-0.9999: 0 ; 0.99-0.999: 0 ; 0.9-0.99: 0 ; <0.9: 0 NREPS 20+: 0 ; 10-19: 0 ; 4-9: 0 ; 2-3: 0 ; 1: 0 MODIFICATIONS None

Total Run Time = 302 seconds. SpectraST finished at Mon Oct 31 14:22:56 2022 without error. Running pipeline command: spectrast2tsv.py -l 400.0,1000.0 -s y,b -d -e -o 6 -n 6 -w swath-windows.txt -k openswath -a SpecLib_cons_openswath.tsv SpecLib_cons.sptxt swathsfile : swath-windows.txt Masslimits: [400.0, 1000.0] Modifications used : ['C[160]', 'M[147]', 'W[202]', 'H[153]', 'K[136]', 'R[166]', 'E[111]', 'Q[111]', 'C[143]', 'n[43]', 'S[167]', 'T[181]', 'Y[243]', 'N[115]', 'Q[129]', 'C[149]', 'D[131]', 'K[144]', 'Y[179]', 'R[172]', 'N[130]', 'P[113]', 'C[119]', 'N[317]', 'N[349]'] Reading : SpecLib_cons.sptxt Traceback (most recent call last): File "/usr/local/bin/spectrast2tsv.py", line 4, in import('pkg_resources').run_script('msproteomicstools==0.6.0', 'spectrast2tsv.py') File "/usr/lib/python3/dist-packages/pkg_resources/init.py", line 667, in run_script self.require(requires)[0].run_script(script_name, ns) File "/usr/lib/python3/dist-packages/pkg_resources/init.py", line 1470, in run_script exec(script_code, namespace, namespace) File "/usr/local/lib/python3.8/dist-packages/msproteomicstools-0.6.0-py3.8.egg/EGG-INFO/scripts/spectrast2tsv.py", line 1263, in File "/usr/local/lib/python3.8/dist-packages/msproteomicstools-0.6.0-py3.8.egg/EGG-INFO/scripts/spectrast2tsv.py", line 867, in main File "/usr/local/lib/python3.8/dist-packages/msproteomicstools-0.6.0-py3.8.egg/msproteomicstoolslib/format/speclib_db_lib.py", line 459, in read_sptxt_with_offset File "/usr/local/lib/python3.8/dist-packages/msproteomicstools-0.6.0-py3.8.egg/msproteomicstoolslib/format/speclib_db_lib.py", line 940, in parse_sptxt AttributeError: 'Spectra' object has no attribute 'name'

sampie commented 1 year ago

Hi,

Log appears to show this warning:

"WARNING: Failed to open mzML file. Output will not contain retention times."

It seems raw files are used directly. I would recommend converting the files to open mzXML and mzML formats according to this protocol paper: https://research.utu.fi/converis/portal/detail/Publication/41624965

Also, there might be various issues if only one sample is being analyzed, I would recommend trying to run the whole set at once.

andrewyy677 commented 1 year ago

Thanks! I’ll try again!

Best, Yingying

从 Windows 版邮件https://go.microsoft.com/fwlink/?LinkId=550986发送

发件人: Sami @.> 发送时间: 2022年11月15日 15:48 收件人: @.> 抄送: Yingying SUN @.>; @.> 主题: Re: [elolab/glaDIAtor] glaDIAtor shut down at log: /opt/comet/comet-ms -P/run-files/analysis xxx (Issue #1)

Hi,

Log appears to show this warning:

"WARNING: Failed to open mzML file. Output will not contain retention times."

It seems raw files are used directly. I would recommend converting the files to open mzXML and mzML formats first by following the conversion shown in this protocol paper: https://research.utu.fi/converis/portal/detail/Publication/41624965https://apc01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fresearch.utu.fi%2Fconveris%2Fportal%2Fdetail%2FPublication%2F41624965&data=05%7C01%7Csunyingying%40westlake.edu.cn%7C21f79c8ef10a422da7b908dac6ddbcd7%7C7e82de2f7ef644169b9644c1457be81b%7C1%7C0%7C638040952908718755%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=umUpb7ENooygplNEBsB%2BSNK4cvS3PUDfp3QlrowVFAA%3D&reserved=0

Also, there might be various issues if only one sample is being analyzed, I would recommend trying to run the whole set at once.

— Reply to this email directly, view it on GitHubhttps://apc01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fgithub.com%2Felolab%2FglaDIAtor%2Fissues%2F1%23issuecomment-1314914206&data=05%7C01%7Csunyingying%40westlake.edu.cn%7C21f79c8ef10a422da7b908dac6ddbcd7%7C7e82de2f7ef644169b9644c1457be81b%7C1%7C0%7C638040952908718755%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=7%2BMnxUhMFsS3Q1NC62U6GBGOpiIgLK6vR7ApHI3H5QY%3D&reserved=0, or unsubscribehttps://apc01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fgithub.com%2Fnotifications%2Funsubscribe-auth%2FA2UR3JKTHJKQEW6IPOPKTTTWIM53NANCNFSM6AAAAAAQA7OWB4&data=05%7C01%7Csunyingying%40westlake.edu.cn%7C21f79c8ef10a422da7b908dac6ddbcd7%7C7e82de2f7ef644169b9644c1457be81b%7C1%7C0%7C638040952908718755%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=cc0MOxoIkx%2BXbC0EAWUOBSVDWt%2BEho5QRS5M9EBsdFI%3D&reserved=0. You are receiving this because you authored the thread.Message ID: @.***>