elsasserlab / minute

MINUTE-ChIP data analysis workflow
https://minute.readthedocs.io
MIT License
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Add a rule to run minute without generating bigwig files #165

Closed cnluzon closed 1 year ago

cnluzon commented 1 year ago

Small PR to allow (and prefer) faster runs:

In some cases (esp. as multiplexing go high and we have a lot of libraries with fewer reads on them) we are more interested in the global quantification than in the bigWig files. On top of that, making the bigWig files with bamCoverage is far from cheap regardless of BAM file size (see #89).

One can always run the bigWig generation afterwards without needing to re-run the previous steps, so I think this also goes a long way towards a smarter use of computation resources. So if one would want to generate bigWig files afterwards, the approach would be minute run no_bigwigs and later on minute run on the same directory.

Since this modification is in the spirit of a faster quantification and makes the most sense for lower coverage BAM files, no_bigwigs also excludes the fingerprint plots.