Open btiernay opened 9 years ago
Getting similar exception using v3.0
java -jar ~/bin/cramtools-3.0/cramtools-3.0.jar bam --input-cram-file HG00096.alt_bwamem_GRCh38DH.20150526.low_coverage.bam.cram --reference-fasta-file /cedar/lin/Data/human/GRCh38_full_analysis_set_plus_decoy_hla.fa --output-bam-file HG00096.alt_bwamem_GRCh38DH.20150526.low_coverage.bam
Exception in thread "main" java.lang.reflect.InvocationTargetException at sun.reflect.NativeMethodAccessorImpl.invoke0(Native Method) at sun.reflect.NativeMethodAccessorImpl.invoke(NativeMethodAccessorImpl.java:62) at sun.reflect.DelegatingMethodAccessorImpl.invoke(DelegatingMethodAccessorImpl.java:43) at java.lang.reflect.Method.invoke(Method.java:497) at net.sf.cram.CramTools.invoke(CramTools.java:91) at net.sf.cram.CramTools.main(CramTools.java:121) Caused by: java.lang.NoClassDefFoundError: org/apache/commons/compress/utils/CountingOutputStream at htsjdk.samtools.cram.build.CramIO.readSAMFileHeader(CramIO.java:254) at htsjdk.samtools.cram.build.CramIO.readCramHeader(CramIO.java:191) at net.sf.cram.common.Utils.openCramInputStream(Utils.java:848) at net.sf.cram.Cram2Bam.main(Cram2Bam.java:107) ... 6 more Caused by: java.lang.ClassNotFoundException: org.apache.commons.compress.utils.CountingOutputStream at java.net.URLClassLoader.findClass(URLClassLoader.java:381) at java.lang.ClassLoader.loadClass(ClassLoader.java:424) at sun.misc.Launcher$AppClassLoader.loadClass(Launcher.java:331) at java.lang.ClassLoader.loadClass(ClassLoader.java:357) ... 10 more
Here too:
INFO 2015-08-29 15:45:29 FixBAMFileHeader Confirming reference sequence md5: 1
INFO 2015-08-29 15:45:29 FixBAMFileHeader Reference sequence MD5 not found, calculating: 2
INFO 2015-08-29 15:45:30 FixBAMFileHeader Reference sequence MD5 not found, calculating: 3
INFO 2015-08-29 15:45:31 FixBAMFileHeader Reference sequence MD5 not found, calculating: 4
INFO 2015-08-29 15:45:31 FixBAMFileHeader Reference sequence MD5 not found, calculating: 5
INFO 2015-08-29 15:45:32 FixBAMFileHeader Reference sequence MD5 not found, calculating: 6
INFO 2015-08-29 15:45:33 FixBAMFileHeader Reference sequence MD5 not found, calculating: 7
INFO 2015-08-29 15:45:33 FixBAMFileHeader Reference sequence MD5 not found, calculating: 8
INFO 2015-08-29 15:45:34 FixBAMFileHeader Reference sequence MD5 not found, calculating: 9
INFO 2015-08-29 15:45:35 FixBAMFileHeader Reference sequence MD5 not found, calculating: 10
INFO 2015-08-29 15:45:35 FixBAMFileHeader Reference sequence MD5 not found, calculating: 11
INFO 2015-08-29 15:45:36 FixBAMFileHeader Reference sequence MD5 not found, calculating: 12
INFO 2015-08-29 15:45:36 FixBAMFileHeader Reference sequence MD5 not found, calculating: 999
Exception in thread "main" java.lang.reflect.InvocationTargetException
at sun.reflect.NativeMethodAccessorImpl.invoke0(Native Method)
at sun.reflect.NativeMethodAccessorImpl.invoke(NativeMethodAccessorImpl.java:62)
at sun.reflect.DelegatingMethodAccessorImpl.invoke(DelegatingMethodAccessorImpl.java:43)
at java.lang.reflect.Method.invoke(Method.java:483)
at net.sf.cram.CramTools.invoke(CramTools.java:91)
at net.sf.cram.CramTools.main(CramTools.java:121)
Caused by: java.lang.NoClassDefFoundError: org/apache/commons/compress/utils/CountingOutputStream
at htsjdk.samtools.cram.build.CramIO.writeContainerForSamFileHeader(CramIO.java:247)
at htsjdk.samtools.cram.build.CramIO.writeCramHeader(CramIO.java:161)
at net.sf.cram.Bam2Cram.main(Bam2Cram.java:405)
... 6 more
Caused by: java.lang.ClassNotFoundException: org.apache.commons.compress.utils.CountingOutputStream
at java.net.URLClassLoader$1.run(URLClassLoader.java:372)
at java.net.URLClassLoader$1.run(URLClassLoader.java:361)
at java.security.AccessController.doPrivileged(Native Method)
at java.net.URLClassLoader.findClass(URLClassLoader.java:360)
at java.lang.ClassLoader.loadClass(ClassLoader.java:424)
at sun.misc.Launcher$AppClassLoader.loadClass(Launcher.java:308)
at java.lang.ClassLoader.loadClass(ClassLoader.java:357)
... 9 more
The reference has an index (X.fasta & X.fasta.fai) The bam file is indexed (Y.bam & Y.bam.bai)
$ java -jar cramtools-3.0/cramtools-3.0.jar
Version 3.0-b30
Same here.
Exception in thread "main" java.lang.reflect.InvocationTargetException at sun.reflect.NativeMethodAccessorImpl.invoke0(Native Method) at sun.reflect.NativeMethodAccessorImpl.invoke(NativeMethodAccessorImpl.java:57) at sun.reflect.DelegatingMethodAccessorImpl.invoke(DelegatingMethodAccessorImpl.java:43) at java.lang.reflect.Method.invoke(Method.java:606) at net.sf.cram.CramTools.invoke(CramTools.java:91) at net.sf.cram.CramTools.main(CramTools.java:121) Caused by: java.lang.NoClassDefFoundError: org/apache/commons/compress/utils/CountingOutputStream at htsjdk.samtools.cram.build.CramIO.readSAMFileHeader(CramIO.java:254) at htsjdk.samtools.cram.build.CramIO.readCramHeader(CramIO.java:191) at net.sf.cram.Cram2Fastq.main(Cram2Fastq.java:90) ... 6 more Caused by: java.lang.ClassNotFoundException: org.apache.commons.compress.utils.CountingOutputStream at java.net.URLClassLoader$1.run(URLClassLoader.java:366) at java.net.URLClassLoader$1.run(URLClassLoader.java:355) at java.security.AccessController.doPrivileged(Native Method) at java.net.URLClassLoader.findClass(URLClassLoader.java:354) at java.lang.ClassLoader.loadClass(ClassLoader.java:425) at sun.misc.Launcher$AppClassLoader.loadClass(Launcher.java:308) at java.lang.ClassLoader.loadClass(ClassLoader.java:358) ... 9 more <<<
I solved the errors by reinstall cramtools from source by following the instruction. Hope it may help you guys. "To build the program from source: To check out the source code from github you will need git client: http://git-scm.com/ Make sure you have java 1.7 or higher: http://openjdk.java.net/ or http://www.oracle.com/us/technologies/java/index.html Make sure you have ant version 1.7 or higher: http://ant.apache.org/ Clone the repository to your local directory: $ git clone https://github.com/enasequence/cramtools.git Change to the directory: $ cd cramtools Build a runnable jar file: $ ant -f build/build.xml runnable Run cramtools $ java -jar cramtools-3.0.jar "
@ChihHsuLin, did not work for me. I also ran the tests without errors.
@ChihHsuLin @kaktus42 Thanks a lot. It works for me. Make sure you build the one from git clone instead of release.
The release tag is missing some dependencies. I've checked in the fixed jar.
Gah, just got stuck on this too. Any chance you could update the latest release to include the dependencies?
Did you try the latest jar from the master branch?
Yes, that worked fine.
next release is coming, hopefully fixing this issue permanently.
I'm currently using the latest release and I'm getting the same error.
Exception in thread "main" java.lang.reflect.InvocationTargetException
at sun.reflect.NativeMethodAccessorImpl.invoke0(Native Method)
at sun.reflect.NativeMethodAccessorImpl.invoke(NativeMethodAccessorImpl.java:57)
at sun.reflect.DelegatingMethodAccessorImpl.invoke(DelegatingMethodAccessorImpl.java:43)
at java.lang.reflect.Method.invoke(Method.java:606)
at net.sf.cram.CramTools.invoke(CramTools.java:91)
at net.sf.cram.CramTools.main(CramTools.java:121)
Caused by: java.lang.NoClassDefFoundError: org/apache/commons/compress/utils/CountingOutputStream
at htsjdk.samtools.cram.build.CramIO.writeContainerForSamFileHeader(CramIO.java:247)
at htsjdk.samtools.cram.build.CramIO.writeCramHeader(CramIO.java:161)
at net.sf.cram.Bam2Cram.main(Bam2Cram.java:405)
... 6 more
Caused by: java.lang.ClassNotFoundException: org.apache.commons.compress.utils.CountingOutputStream
at java.net.URLClassLoader$1.run(URLClassLoader.java:366)
at java.net.URLClassLoader$1.run(URLClassLoader.java:355)
at java.security.AccessController.doPrivileged(Native Method)
at java.net.URLClassLoader.findClass(URLClassLoader.java:354)
at java.lang.ClassLoader.loadClass(ClassLoader.java:425)
at sun.misc.Launcher$AppClassLoader.loadClass(Launcher.java:308)
at java.lang.ClassLoader.loadClass(ClassLoader.java:358)
[root@localhost cramtools]# java -jar cramtools-3.0.jar bam --input-cram-file /usr/snp/cramtools/HG00096.alt_bwamem_GRCh38DH.20150718.GBR.low_coverage.cram --reference-fasta-file /usr/snp/cramtools/GRCh38_full_analysis_set_plus_decoy_hla.fa --skip-md5-check --output-bam-file HG00096.bam Exception in thread "main" java.lang.reflect.InvocationTargetException at sun.reflect.NativeMethodAccessorImpl.invoke0(Native Method) at sun.reflect.NativeMethodAccessorImpl.invoke(NativeMethodAccessorImpl.java:62) at sun.reflect.DelegatingMethodAccessorImpl.invoke(DelegatingMethodAccessorImpl.java:43) at java.lang.reflect.Method.invoke(Method.java:498) at net.sf.cram.CramTools.invoke(CramTools.java:91) at net.sf.cram.CramTools.main(CramTools.java:121) Caused by: java.lang.OutOfMemoryError: Java heap space at java.util.Arrays.copyOf(Arrays.java:3236) at java.io.ByteArrayOutputStream.grow(ByteArrayOutputStream.java:118) at java.io.ByteArrayOutputStream.ensureCapacity(ByteArrayOutputStream.java:93) at java.io.ByteArrayOutputStream.write(ByteArrayOutputStream.java:153) at htsjdk.samtools.util.IOUtil.copyStream(IOUtil.java:658) at htsjdk.samtools.cram.io.InputStreamUtils.readFully(InputStreamUtils.java:40) at net.sf.cram.ref.ReferenceSource.loadFromPath(ReferenceSource.java:254) at net.sf.cram.ref.ReferenceSource.findBasesByMD5(ReferenceSource.java:287) at net.sf.cram.ref.ReferenceSource.findBases(ReferenceSource.java:180) at net.sf.cram.ref.ReferenceSource.getReferenceBases(ReferenceSource.java:128) at net.sf.cram.Cram2Bam.main(Cram2Bam.java:220) ... 6 more [root@localhost cramtools]# ls
@wangying0128 please try with more memory: java -Xmx4g -jar cramtools-3.0.jar bam --input-cram-file /usr/snp/cramtools/HG00096.alt_bwamem_GRCh38DH.20150718.GBR.low_coverage.cram --reference-fasta-file /usr/snp/cramtools/GRCh38_full_analysis_set_plus_decoy_hla.fa --skip-md5-check --output-bam-file HG00096.bam
I also had the same problem, building from the repo fixed it.
Getting the same error. adding more memory did not fix it.
Getting the same error. Cloning from git and building fixed it!
Because this thread helped >1 year after, I also fixed error by cloning git and building with
ant -f build/build.xml runnable
Getting this exception using the versioned download in v3.0 (https://github.com/enasequence/cramtools/archive/v3.0.tar.gz):