enasequence / enaBrowserTools

A collection of scripts to assist in the retrieval of data from the ENA Browser
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Error when downloading raw sequencing files from a registered project. #65

Closed padpadpadpad closed 1 year ago

padpadpadpad commented 3 years ago

Hi ENA team

I am trying to download all the sequences from this project. Its project accession code is PRJEB47945.

To do this I use the code:

enaGroupGet -d ena_test -f fastq -g read PRJEB47945

Some of the files do download, but lots of the files give the following error:

Fetching ERR7017432
Downloading file with md5 check:ftp.sra.ebi.ac.uk/vol1/fastq/ERR701/002/ERR7017432/ERR7017432_1.fastq.gz
Downloading file with md5 check:ftp.sra.ebi.ac.uk/vol1/fastq/ERR701/002/ERR7017432/ERR7017432_2.fastq.gz
Error with FTP transfer occurred for file: ERR7017431_2.fastq.gzError with FTP transfer: <urlopen error ftp error: error_perm('550 Failed to change directory.')>
Downloading file with md5 check:ftp.sra.ebi.ac.uk/vol1/fastq/ERR701/002/ERR7017432/ERR7017432_2.fastq.gz
Error with FTP transfer occurred for file: ERR7017432_2.fastq.gzError with FTP transfer: <urlopen error ftp error: error_perm('550 Failed to change directory.')>
Failed to download file after two attempts

The output is from one sample where the forward read did download but the reverse read did not.

Any idea how to fix the Error with FTP transfer: <urlopen error ftp error: error_perm('550 Failed to change directory.')>

Many thanks Dan

lilim-ebi commented 1 year ago

Hi,

Are you still experiencing this problem? It works fine for me, I'm using version 1.5.4.