epi2me-labs / modbam2bed

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Working with Dorado Basecaller #46

Closed ethan-mcq closed 1 year ago

ethan-mcq commented 1 year ago

Does this tool only work with the Guppy basecaller? I used the Dorado basecaller like so:

@PG     ID:basecaller   PN:dorado       VN:0.1.1+eb48766        CL:dorado basecaller dorado-0.1.1/dna_r10.4.1_e8.2_400bps_hac@v4.0.0 fast5s --modified-bases 5mCG_5hmCG

I can't seem to get it to work, although the test data works just fine. Thanks for the help in advance!

cjw85 commented 1 year ago

The program will work with any BAM file containing aligned reads and MM and ML tags.