Hello,
I'm working on aligning cDNA RNA-seq ONT data. I noticed that the "--minimap_preset rna" parameter is designed for Nanopore Direct RNA-seq. Do I need to adjust the code in the Nextflow script for my datasets?
There's no need to change the code. You can provide arguments to be passed to minimap2 with the --minimap_args parameter (please note that this will override any preset arguments though).
Ask away!
Hello, I'm working on aligning cDNA RNA-seq ONT data. I noticed that the "--minimap_preset rna" parameter is designed for Nanopore Direct RNA-seq. Do I need to adjust the code in the Nextflow script for my datasets?