epi2me-labs / wf-artic

ARTIC SARS-CoV-2 workflow and reporting
https://labs.epi2me.io/
Other
47 stars 34 forks source link

No nextclade.tsv output #102

Closed rgn3210 closed 9 months ago

rgn3210 commented 10 months ago

Operating System

Ubuntu 22.04

Other Linux

No response

Workflow Version

v.0.3.32

Workflow Execution

Command line

EPI2ME Version

No response

CLI command run

nextflow run epi2me-labs/wf-artic --update_data --fastq ./fastq_pass --sample_sheet samplesheet.csv --scheme_version Midnight-ONT/V1 --min_len 200 --max_len 1300

Workflow Execution - CLI Execution Profile

None

What happened?

I recently updated my wf-artic version from v0.3.21 to the newest v.0.3.32, however when i ran my samples I didnt have the nextclade.tsv output. Otherwise the workflow ran as usual. In the .html report the lineages are speciafied, the analysis ran correctly. What could be causing this issue?

Relevant log output

N E X T F L O W  ~  version 23.04.2
Launching `https://github.com/epi2me-labs/wf-artic` [clever_keller] DSL2 - revision: 443c9f0e1b [master]

||||||||||   _____ ____ ___ ____  __  __ _____      _       _
||||||||||  | ____|  _ \_ _|___ \|  \/  | ____|    | | __ _| |__  ___
|||||       |  _| | |_) | |  __) | |\/| |  _| _____| |/ _` | '_ \/ __|
|||||       | |___|  __/| | / __/| |  | | |__|_____| | (_| | |_) \__ \
||||||||||  |_____|_|  |___|_____|_|  |_|_____|    |_|\__,_|_.__/|___/
||||||||||  wf-artic v0.3.32-g443c9f0
--------------------------------------------------------------------------------
Core Nextflow options
  revision       : master
  runName        : clever_keller
  containerEngine: docker
  launchDir      : /data2/Storage/ANALYSIS/SARS-CoV-2/test
  workDir        : /data2/Storage/ANALYSIS/SARS-CoV-2/test/work
  projectDir     : /home/user/.nextflow/assets/epi2me-labs/wf-artic
  userName       : user
  profile        : standard
  configFiles    : /home/user/.nextflow/assets/epi2me-labs/wf-artic/nextflow.config

Input Options
  fastq          : ./fastq_pass

Primer Scheme Selection
  scheme_version : Midnight-ONT/V1

Sample Options
  sample_sheet   : samplesheet.csv

Advanced Options
  min_len        : 200
  max_len        : 1300

!! Only displaying parameters that differ from the pipeline defaults !!
--------------------------------------------------------------------------------
If you use epi2me-labs/wf-artic for your analysis please cite:

* The nf-core framework
  https://doi.org/10.1038/s41587-020-0439-x

--------------------------------------------------------------------------------
This is epi2me-labs/wf-artic v0.3.32-g443c9f0.
--------------------------------------------------------------------------------

      ------------------------------------
      Available Primer Schemes:
      ------------------------------------

  Name      Version
  SARS-CoV-2    NEB-VarSkip/v1a 
  SARS-CoV-2    NEB-VarSkip/v2b 
  SARS-CoV-2    NEB-VarSkip/v1a-long    
  SARS-CoV-2    NEB-VarSkip/v2  
  SARS-CoV-2    ARTIC/V2    
  SARS-CoV-2    ARTIC/V5.3.2    
  SARS-CoV-2    ARTIC/V4.1  
  SARS-CoV-2    ARTIC/V1    
  SARS-CoV-2    ARTIC/V3    
  SARS-CoV-2    ARTIC/V4    
  SARS-CoV-2    Midnight-ONT/V2 
  SARS-CoV-2    Midnight-ONT/V1 
  SARS-CoV-2    Midnight-ONT/V3 
  SARS-CoV-2    Midnight-IDT/V2 
  SARS-CoV-2    Midnight-IDT/V1 

      ------------------------------------

Searching input for [.fastq, .fastq.gz, .fq, .fq.gz] files.
executor >  local (32)
[31/84610b] process > validate_sample_sheet                    [100%] 1 of 1 ✔
[c3/8abf5a] process > fastcat (2)                              [100%] 2 of 2 ✔
[0a/d2e1f2] process > pipeline:getVersions                     [100%] 1 of 1 ✔
[b4/6bc622] process > pipeline:getParams                       [100%] 1 of 1 ✔
[01/b4827a] process > pipeline:lookup_medaka_variant_model (1) [100%] 1 of 1 ✔
[a9/7a027c] process > pipeline:runArtic (2)                    [100%] 2 of 2 ✔
[85/8931f9] process > pipeline:combineDepth                    [100%] 1 of 1 ✔
[2e/92a3bb] process > pipeline:allConsensus                    [100%] 1 of 1 ✔
[be/1081f4] process > pipeline:allVariants                     [100%] 1 of 1 ✔
[55/c7a176] process > pipeline:prep_nextclade                  [100%] 1 of 1 ✔
[ab/6fa433] process > pipeline:nextclade                       [100%] 1 of 1 ✔
[7f/e7a73b] process > pipeline:pangolin                        [100%] 1 of 1 ✔
[52/05e589] process > pipeline:report                          [100%] 1 of 1 ✔
[2c/615c15] process > output (15)                              [100%] 17 of 17 ✔
Completed at: 17-Nov-2023 14:25:34
Duration    : 1m 29s
CPU hours   : 0.1
Succeeded   : 32

Application activity log entry

No response