I have encountered some inconsistencies when running the wf-artic pipeline.
Sometimes the pipeline is running without any issues, wheres on other occasions I get the error below, which appears to be caused by a failed pipeline:getVersions, and I am not sure what is causing it?
Does anyone know what the issue could be?
Thanks a lot in advance.
$nextflow run epi2me-labs/wf-artic -w ${OUTPUT}/workspace -profile conda --fastq /path/to/reads/fastq_demultiplexed --samples /path/to/sample/sheet/sample_sheet --out_dir ${OUTPUT}
N E X T F L O W ~ version 21.10.4
WARN: Access to undefined parameter detect_samples -- Initialise it to a default value eg. params.detect_samples = some_value
Checking sample sheet.
executor > local (8)
[8d/de2916] process > checkSampleSheet (1) [100%] 1 of 1 ✔
executor > local (8)
[8d/de2916] process > checkSampleSheet (1) [100%] 1 of 1 ✔
[88/63dc7b] process > pipeline:getVersions [100%] 1 of 1, failed: 1 ✘
[1a/bf87f8] process > pipeline:getParams [100%] 1 of 1 ✔
[35/7951d4] process > pipeline:copySchemeDir [100%] 1 of 1 ✔
[c4/64a846] process > pipeline:preArticQC (3) [ 0%] 0 of 1
[- ] process > pipeline:runArtic [ 0%] 0 of 5
[- ] process > pipeline:allConsensus -
[- ] process > pipeline:allVariants -
[- ] process > pipeline:nextclade -
[- ] process > pipeline:pangolin -
[- ] process > pipeline:telemetry -
[- ] process > pipeline:report -
[- ] process > output -
Checking input directory structure.
Found barcode directories
Error executing process > 'pipeline:getVersions'
Caused by:
Process pipeline:getVersions terminated with an error exit status (141)
Command executed:
medaka --version | sed 's/ /,/' >> versions.txt
minimap2 --version | sed 's/^/minimap2,/' >> versions.txt
bcftools --version | head -n 1 | sed 's/ /,/' >> versions.txt
samtools --version | head -n 1 | sed 's/ /,/' >> versions.txt
nextclade --version | sed 's/^/nextclade,/' >> versions.txt
artic --version | sed 's/ /,/' >> versions.txt
Command exit status:
141
Command output:
(empty)
Work dir:
${OUTPUT}/workspace/88/63dc7bd7dd452b057b7b440902af74
Tip: view the complete command output by changing to the process work dir and entering the command cat .command.out
WARN: To render the execution DAG in the required format it is required to install Graphviz -- See http://www.graphviz.org for more info.
I have encountered some inconsistencies when running the wf-artic pipeline. Sometimes the pipeline is running without any issues, wheres on other occasions I get the error below, which appears to be caused by a failed
pipeline:getVersions
, and I am not sure what is causing it?Does anyone know what the issue could be?
Thanks a lot in advance.
$nextflow run epi2me-labs/wf-artic -w ${OUTPUT}/workspace -profile conda --fastq /path/to/reads/fastq_demultiplexed --samples /path/to/sample/sheet/sample_sheet --out_dir ${OUTPUT}
N E X T F L O W ~ version 21.10.4 WARN: Access to undefined parameterdetect_samples
-- Initialise it to a default value eg.params.detect_samples = some_value
Checking sample sheet. executor > local (8) [8d/de2916] process > checkSampleSheet (1) [100%] 1 of 1 ✔ executor > local (8) [8d/de2916] process > checkSampleSheet (1) [100%] 1 of 1 ✔ [88/63dc7b] process > pipeline:getVersions [100%] 1 of 1, failed: 1 ✘ [1a/bf87f8] process > pipeline:getParams [100%] 1 of 1 ✔ [35/7951d4] process > pipeline:copySchemeDir [100%] 1 of 1 ✔ [c4/64a846] process > pipeline:preArticQC (3) [ 0%] 0 of 1 [- ] process > pipeline:runArtic [ 0%] 0 of 5 [- ] process > pipeline:allConsensus - [- ] process > pipeline:allVariants - [- ] process > pipeline:nextclade - [- ] process > pipeline:pangolin - [- ] process > pipeline:telemetry - [- ] process > pipeline:report - [- ] process > output - Checking input directory structure.Caused by: Process
pipeline:getVersions
terminated with an error exit status (141)Command executed:
medaka --version | sed 's/ /,/' >> versions.txt minimap2 --version | sed 's/^/minimap2,/' >> versions.txt bcftools --version | head -n 1 | sed 's/ /,/' >> versions.txt samtools --version | head -n 1 | sed 's/ /,/' >> versions.txt nextclade --version | sed 's/^/nextclade,/' >> versions.txt artic --version | sed 's/ /,/' >> versions.txt
Command exit status: 141
Command output: (empty)
Work dir: ${OUTPUT}/workspace/88/63dc7bd7dd452b057b7b440902af74
Tip: view the complete command output by changing to the process work dir and entering the command
cat .command.out
WARN: To render the execution DAG in the required format it is required to install Graphviz -- See http://www.graphviz.org for more info.