EPI2ME Error : Validation of pipeline parameters failed!

[handoko12u]

Ask away!

Below is my command:

sysadmin@sysadmin:~/Downloads/nextflow$ nextflow run epi2me-labs/wf-human-variation –help N E X T F L O W ~ version 23.10.0 NOTE: Your local project version looks outdated - a different revision is available in the remote repository [04c3b27058] Launching https://github.com/epi2me-labs/wf-human-variation [happy_church] DSL2 - revision: 91dc78c063 [master]

ERROR ~ ERROR: Validation of pipeline parameters failed!

-- Check '.nextflow.log' file for details ERROR ~ * Bad parameter configuration. You must select at least one of: --sv --snp --cnv --str --methyl

-- Check '.nextflow.log' file for details ERROR ~ * Missing required parameter: --ref

-- Check '.nextflow.log' file for details ERROR ~ * Bad parameter configuration. You must select only one option of: --fast5_dir --bam

-- Check '.nextflow.log' file for details

Questions:

1. When running this command, I suppose it will show help, displaying various options available, but why it turned out to be error?

2. It stated my local project looks outdated, what should I do? Prior to this, I have updated my nextflow to version 23.10.0.5889.

Please anyone can help me?

Thank you very much

[hyunjokoo]

1. Please make sure you typed --help instead of –help.

2. The version is not about Nestflow but about wf-human-variation. I think wf-human-variation is updated automatically. Although v1.9.2 was released, my version is still v1.9.0 and v1.9.1 in different severs. I think it takes some time for the update.

[cjw85]

Nextflow workflows are not updated automatically by nextflow. See the Nextflow documentation for how to update workflows.

[hyunjokoo]

Nextflow workflows are not updated automatically by nextflow. See the Nextflow documentation for how to update workflows.

Oh. Thank you. Good information!!