PCR duplicates removal - Githubissues

epi2me-labs / wf-human-variation

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PCR duplicates removal #155

Closed seven1112233 closed 3 months ago

seven1112233 commented 3 months ago

Ask away!

Hi, If we call SNPs in the nanopore cDNA sequencing data, should we perform PCR removal? I am worried that the PCR amplifying would affect variants calling

cjw85 commented 3 months ago

The workflow is not intended for use with cDNA sequencing data, but rather whole genome native DNA.