Closed nikelau closed 1 month ago
Hi @nikelau thanks for your interest in the workflow. Can I please check, are you running the workflow on adaptive sampling data? If so then we would recommend trying QDNAseq instead of Spectre, as the latter isn't suitable for use with adaptive sampling.
oh thanks Sirisha! yes i am using adaptive sampling. let me try again. by the way i tried the same set of data on two different windows machines (slightly different specs), while the other one (after deleting the TR BED as in another issue on github) worked using QDNAseq. it can sometimes be inconsistent even in terms of which bug will occur. adaptive sampling is great for low cost human applications, and it would be great if there could be specific workflows that provides best tools and settings for e.g. exomes or panels in addition to human genome. please consider! thanks!
Hi, thank you for your comment re: adaptive sampling and exams/panels. We are in the process of revising our documentation around the options/settings which can be used for both Spectre and QDNAseq so please keep an eye on the repo for updates.
I'll close this issue for now but please feel free to re-open or create a new issue if you have any further problems with CNV calling.
Operating System
Windows 11
Other Linux
No response
Workflow Version
v2.2.6
Workflow Execution
EPI2ME Desktop (Local)
Other workflow execution
No response
EPI2ME Version
V5.1.14
CLI command run
No response
Workflow Execution - CLI Execution Profile
None
What happened?
ERROR ~ Error executing process > 'cnv_spectre:callCNV (1)'
I am running this workflow on another computer using the same configurations and input files as the other error request with sniffles.
Relevant log output
Application activity log entry
Were you able to successfully run the latest version of the workflow with the demo data?
yes
Other demo data information
No response