Closed EsbergA closed 1 year ago
Hi! Thank you for using the workflow! It may be something related with your data. Could you send me the next files so that I can investigate what is happening.
In the EPI2ME Desktop application go to "Track Analyses
" (in the dashboard), choose the one that has been "stopped with Error
" and click on "Open folder
", then move to the directory:work/9a/b9812e3a3d4426f3e6a1e5644776c1
. It should be a folder called 'lineages
'
Could you send me the content of that folder? You can zip it to send it through GitHub
Thank you for a quick answer. Attached is the Lineages / Anders
Från: Natalia Garcia Garcia @.> Datum: fredag, 22 september 2023 09:54 Till: epi2me-labs/wf-metagenomics @.> Kopia: Anders Esberg @.>, Author @.> Ämne: Re: [epi2me-labs/wf-metagenomics] wf-metagenomic: Minimap error 16S_18S NCBI database (Issue #64)
Hi! Thank you for using the workflow! It may be something related with your data. Could you send me the next files so that I can investigate what is happening.
In the EPI2ME Desktop application go to "Track Analyses" (in the dashboard), choose the one that has been "stopped with Error" and click on "Open folder", then move to the directory: work/9a/b9812e3a3d4426f3e6a1e5644776c1. It should be a folder called 'lineages'
Could you send me the content of that folder? You can zip it to send it through GitHub
— Reply to this email directly, view it on GitHubhttps://github.com/epi2me-labs/wf-metagenomics/issues/64#issuecomment-1730970845, or unsubscribehttps://github.com/notifications/unsubscribe-auth/BCRRRSDNPVJ77N37VZYAMWLX3U73ZANCNFSM6AAAAAA5CT6LYM. You are receiving this because you authored the thread.Message ID: @.***>
Apologies, but I don't see any attached file
Hi again Were you able to identify the issue? I tried the wf-16S work-flow with the same issue. / A
Hi,
Apologies but I haven't found it yet. I suspect that one (or more) of the files contained in the lineages
folder are empty. They should contain the different lineages identified and the counts. However, I could check it because they were symbolic links. I will add something to identify the barcode which is failing but meanwhile, could you check it or zip the files:
barcode90.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/61/f6db3bb23b39d0118054f2e3d597a3/barcode90.json barcode86.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/ed/d319fefded353a7b4c3c2c68a552bc/barcode86.json barcode73.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/5e/3af0c97cd3cd74e7736ca415bae1fd/barcode73.json barcode69.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/28/3ef3d5bae9bc0e3724023711618ce3/barcode69.json barcode53.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/eb/ef980386f0918682353ca750031ba0/barcode53.json barcode45.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/53/b5844636bf290b0f24b7d30b61db90/barcode45.json barcode28.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/50/c004a42aa1716f862726524ed9f539/barcode28.json barcode24.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/e9/b4129010390ddfb4048793b2a2f553/barcode24.json barcode12.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/90/eb5c0a408ee1944d894a911bffd4b0/barcode12.json barcode04.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/fa/a4c14097955e2c964f2005435779df/barcode04.json barcode96.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/ed/667ddad9c665021ef637561b7a9925/barcode96.json barcode91.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/fc/dd7e57e71f7a6a755ea7969f42ed4e/barcode91.json barcode87.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/fb/0984dd6268294e3492ad890cd0c87c/barcode87.json barcode81.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/95/de9b56de36ec7c71e73ba8bc1679c5/barcode81.json barcode80.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/cc/1d81b6cc144e5353121e34e31eacc6/barcode80.json barcode79.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/55/da0d714f266c503e2ea65507763324/barcode79.json barcode78.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/01/11fb60e0648a6d187796f607c37c97/barcode78.json barcode75.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/04/35fb629e0ad841b0a8175bf3956484/barcode75.json barcode72.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/35/1bbb1018e17913aa5182ecf677d9da/barcode72.json barcode68.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/6b/53d86edad6642d82f9d11d5978f894/barcode68.json barcode65.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/6b/58f1dd5a4d04f26209ca1feeddfa27/barcode65.json barcode64.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/c1/102d4f2001f2615ccd25726ec743cf/barcode64.json barcode63.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/f9/456e66f5185dcfc1043f2487b0cdbd/barcode63.json barcode59.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/00/bb40c87f282c624f257ce78c225e27/barcode59.json barcode58.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/80/335f8c02e68f4c0f5c87fd70bcc2a9/barcode58.json barcode55.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/e5/afca48eccebfdce98a31781f23c073/barcode55.json barcode54.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/94/3b17ce4f35aade888e119d794dd3d3/barcode54.json barcode52.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/d6/6a64dabb1705f19836f0adf07caa14/barcode52.json barcode49.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/1e/a073e64ff5e84cf4212f29df924b51/barcode49.json barcode48.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/68/04ae4f091ba7efdbafc91dcb7f9ebf/barcode48.json barcode44.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/6f/1b11caacf5c1c012a6f2b3c7ede527/barcode44.json barcode43.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/fc/a59304b0523610bda45404bb0888af/barcode43.json barcode42.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/a3/44ff7111f9b13d09e3f2672fcfb877/barcode42.json barcode39.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/80/50a76f4b0f43d25dcb1d153582159d/barcode39.json barcode38.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/6a/6073a698c2fa32c604fc6726cbc9e5/barcode38.json barcode34.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/07/e0f652fe2ce3569d6a4d72ac38fed7/barcode34.json barcode33.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/02/6442d3c56ef1bf43eb86a8e9ea7a77/barcode33.json barcode32.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/a2/a5b1d51e2ac6a479fef9b8ea0bd457/barcode32.json barcode29.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/22/d37929247a34470fe7ab8996d76f11/barcode29.json barcode25.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/6e/d61562bdffe172e768c9ad14bd928e/barcode25.json barcode22.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/7a/dd2494ad8ff9f3bb067ac5ec9cde96/barcode22.json barcode18.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/d7/979d0dcaa6cde93fb101cbd688bea9/barcode18.json barcode15.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/98/6ac37421617e573f3624da0913e3aa/barcode15.json barcode14.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/51/ac63747d3db7fcd056e5cc64344857/barcode14.json barcode13.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/4d/ea11a58589f22f6c591d55dc289b01/barcode13.json barcode09.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/73/353747b6458d7f06ca5c27819d5566/barcode09.json barcode08.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/eb/b6bb545e69af6a540aed01b7b5117f/barcode08.json barcode05.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/c4/db61ed388e6675af591ea0eb8f8bde/barcode05.json barcode03.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/4c/34da8c5bbb8bf7f06f2ebff466565c/barcode03.json barcode02.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/09/3b20e56dd162ebfceb6fb4111737c6/barcode02.json barcode95.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/b0/db4e0be15f9f12eae4e114a60d38f2/barcode95.json barcode94.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/a5/41870c1373d06adac4fe79e8c245e7/barcode94.json barcode93.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/c1/871f1adcda8e02aaf59c70bcda4fa9/barcode93.json barcode92.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/49/1c039d286b05b35f1f7e9c0bee9b15/barcode92.json barcode89.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/fe/f18f6418b3465e3d9926c73dea1d8f/barcode89.json barcode88.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/74/09041775532d8a7e4ae1d7f8317fed/barcode88.json barcode85.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/64/336afd252de3e5ad033d2c0fc76504/barcode85.json barcode84.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/33/3cea8f10d477a39f05a519b90f0cf4/barcode84.json barcode83.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/ef/d40e44e424ec1a00b8f33f0887a90c/barcode83.json barcode82.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/86/bc73592172d400713cf32df29262ed/barcode82.json barcode77.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/05/d9a217b9960a440c1cf3dc78cde96e/barcode77.json barcode76.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/20/8cd16f77811e37997782a4d224b099/barcode76.json barcode74.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/54/318dd8e66a93d59f8fb08174b59772/barcode74.json barcode71.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/aa/7c8b2a2c6109c7a1ee051e3c301d42/barcode71.json barcode70.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/8b/beeeb087bfc1b90e1d8b703407bba6/barcode70.json barcode67.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/23/b5c28fa34510153a49e124979c20c1/barcode67.json barcode66.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/35/4aa2e83fd1b51dbd50b953d0ed59a4/barcode66.json barcode62.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/fd/9048bb5b2cffa7ec95482f51ac952f/barcode62.json barcode61.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/15/4f3566e4e2539055cea255f13d07f4/barcode61.json barcode60.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/13/cb4d0b361491ca273cae20465b6daa/barcode60.json barcode57.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/03/74ceda9c9d49cd1675ef922aecc2af/barcode57.json barcode56.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/03/81fcaaf736df8626501cce8e09f17d/barcode56.json barcode51.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/f1/4ec8e304ca588b1344b625bba9009c/barcode51.json barcode50.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/c6/3e33e1da0815594571b51973c4a79b/barcode50.json barcode47.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/c4/6ed7bc78b383aad6f65b790ac04b55/barcode47.json barcode46.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/1c/92450d07e308c1f391e9739386d566/barcode46.json barcode41.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/1d/9b33fa86ec50c78ac737fde362a3d1/barcode41.json barcode40.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/1c/008344ec69632ca9cef084453551eb/barcode40.json barcode37.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/9f/77faf44b43053827de11490d922458/barcode37.json barcode36.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/fd/95f5fbab00748048c873217646777e/barcode36.json barcode35.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/94/316d8b31bfaa1728ad12ca0b133d0c/barcode35.json barcode31.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/b1/5f580a8bf1fa2facff28b7ba4ac3d3/barcode31.json barcode30.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/48/56dcf55f01d32ef5c0d0f289c61414/barcode30.json barcode27.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/57/d5ac254a439dff833fc5829d56012a/barcode27.json barcode26.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/a6/7eae0e2215e3fff28c91bb22e1f87c/barcode26.json barcode23.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/ec/20f985fbe1800c24a1491bca68fe8e/barcode23.json barcode21.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/26/0ce04b1ef19e87767bf1c42b673ca0/barcode21.json barcode20.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/86/c591458aafc31cb1b44f32fbd7b921/barcode20.json barcode19.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/d7/16de22801cc813a18d5bf5d680b77a/barcode19.json barcode17.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/17/3b23681fbbaf2be8fc7b0d315bef0b/barcode17.json barcode16.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/24/5bdff7663b09c2a61e90c5b7f6014d/barcode16.json barcode11.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/83/17c4544b0b5fc6d22a785cb34269c3/barcode11.json barcode10.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/df/d54fced364361daea4b88f7cc0fc08/barcode10.json barcode06.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/9d/dba732dd965c6ab9bedfc4268be0e8/barcode06.json barcode01.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/34/ef85323b2264f0cd197e44a185bfc4/barcode01.json barcode07.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/76/3458ea0f4fae5d1d6451b08945a07d/barcode07.json
Ok, this could make sense, as some folders may be totally empty, I will look through the folders. I run 96 barcoded samples at the time including negative and positive samples and some were not used. /A
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Från: Natalia Garcia Garcia @.> Skickat: Monday, September 25, 2023 10:19:31 AM Till: epi2me-labs/wf-metagenomics @.> Kopia: Anders Esberg @.>; Author @.> Ämne: Re: [epi2me-labs/wf-metagenomics] wf-metagenomic: Minimap error 16S_18S NCBI database (Issue #64)
Hi, Apologies but I haven't found it yet. I suspect that one of the files contained in the lineages folder is empty. However, I could check it because they were symbolic links. I will add something to identify the barcode which is failing but meanwhile, could you check it or zip the files:
barcode90.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/61/f6db3bb23b39d0118054f2e3d597a3/barcode90.json barcode86.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/ed/d319fefded353a7b4c3c2c68a552bc/barcode86.json barcode73.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/5e/3af0c97cd3cd74e7736ca415bae1fd/barcode73.json barcode69.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/28/3ef3d5bae9bc0e3724023711618ce3/barcode69.json barcode53.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/eb/ef980386f0918682353ca750031ba0/barcode53.json barcode45.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/53/b5844636bf290b0f24b7d30b61db90/barcode45.json barcode28.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/50/c004a42aa1716f862726524ed9f539/barcode28.json barcode24.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/e9/b4129010390ddfb4048793b2a2f553/barcode24.json barcode12.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/90/eb5c0a408ee1944d894a911bffd4b0/barcode12.json barcode04.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/fa/a4c14097955e2c964f2005435779df/barcode04.json barcode96.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/ed/667ddad9c665021ef637561b7a9925/barcode96.json barcode91.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/fc/dd7e57e71f7a6a755ea7969f42ed4e/barcode91.json barcode87.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/fb/0984dd6268294e3492ad890cd0c87c/barcode87.json barcode81.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/95/de9b56de36ec7c71e73ba8bc1679c5/barcode81.json barcode80.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/cc/1d81b6cc144e5353121e34e31eacc6/barcode80.json barcode79.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/55/da0d714f266c503e2ea65507763324/barcode79.json barcode78.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/01/11fb60e0648a6d187796f607c37c97/barcode78.json barcode75.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/04/35fb629e0ad841b0a8175bf3956484/barcode75.json barcode72.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/35/1bbb1018e17913aa5182ecf677d9da/barcode72.json barcode68.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/6b/53d86edad6642d82f9d11d5978f894/barcode68.json barcode65.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/6b/58f1dd5a4d04f26209ca1feeddfa27/barcode65.json barcode64.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/c1/102d4f2001f2615ccd25726ec743cf/barcode64.json barcode63.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/f9/456e66f5185dcfc1043f2487b0cdbd/barcode63.json barcode59.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/00/bb40c87f282c624f257ce78c225e27/barcode59.json barcode58.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/80/335f8c02e68f4c0f5c87fd70bcc2a9/barcode58.json barcode55.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/e5/afca48eccebfdce98a31781f23c073/barcode55.json barcode54.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/94/3b17ce4f35aade888e119d794dd3d3/barcode54.json barcode52.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/d6/6a64dabb1705f19836f0adf07caa14/barcode52.json barcode49.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/1e/a073e64ff5e84cf4212f29df924b51/barcode49.json barcode48.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/68/04ae4f091ba7efdbafc91dcb7f9ebf/barcode48.json barcode44.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/6f/1b11caacf5c1c012a6f2b3c7ede527/barcode44.json barcode43.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/fc/a59304b0523610bda45404bb0888af/barcode43.json barcode42.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/a3/44ff7111f9b13d09e3f2672fcfb877/barcode42.json barcode39.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/80/50a76f4b0f43d25dcb1d153582159d/barcode39.json barcode38.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/6a/6073a698c2fa32c604fc6726cbc9e5/barcode38.json barcode34.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/07/e0f652fe2ce3569d6a4d72ac38fed7/barcode34.json barcode33.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/02/6442d3c56ef1bf43eb86a8e9ea7a77/barcode33.json barcode32.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/a2/a5b1d51e2ac6a479fef9b8ea0bd457/barcode32.json barcode29.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/22/d37929247a34470fe7ab8996d76f11/barcode29.json barcode25.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/6e/d61562bdffe172e768c9ad14bd928e/barcode25.json barcode22.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/7a/dd2494ad8ff9f3bb067ac5ec9cde96/barcode22.json barcode18.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/d7/979d0dcaa6cde93fb101cbd688bea9/barcode18.json barcode15.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/98/6ac37421617e573f3624da0913e3aa/barcode15.json barcode14.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/51/ac63747d3db7fcd056e5cc64344857/barcode14.json barcode13.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/4d/ea11a58589f22f6c591d55dc289b01/barcode13.json barcode09.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/73/353747b6458d7f06ca5c27819d5566/barcode09.json barcode08.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/eb/b6bb545e69af6a540aed01b7b5117f/barcode08.json barcode05.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/c4/db61ed388e6675af591ea0eb8f8bde/barcode05.json barcode03.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/4c/34da8c5bbb8bf7f06f2ebff466565c/barcode03.json barcode02.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/09/3b20e56dd162ebfceb6fb4111737c6/barcode02.json barcode95.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/b0/db4e0be15f9f12eae4e114a60d38f2/barcode95.json barcode94.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/a5/41870c1373d06adac4fe79e8c245e7/barcode94.json barcode93.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/c1/871f1adcda8e02aaf59c70bcda4fa9/barcode93.json barcode92.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/49/1c039d286b05b35f1f7e9c0bee9b15/barcode92.json barcode89.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/fe/f18f6418b3465e3d9926c73dea1d8f/barcode89.json barcode88.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/74/09041775532d8a7e4ae1d7f8317fed/barcode88.json barcode85.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/64/336afd252de3e5ad033d2c0fc76504/barcode85.json barcode84.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/33/3cea8f10d477a39f05a519b90f0cf4/barcode84.json barcode83.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/ef/d40e44e424ec1a00b8f33f0887a90c/barcode83.json barcode82.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/86/bc73592172d400713cf32df29262ed/barcode82.json barcode77.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/05/d9a217b9960a440c1cf3dc78cde96e/barcode77.json barcode76.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/20/8cd16f77811e37997782a4d224b099/barcode76.json barcode74.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/54/318dd8e66a93d59f8fb08174b59772/barcode74.json barcode71.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/aa/7c8b2a2c6109c7a1ee051e3c301d42/barcode71.json barcode70.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/8b/beeeb087bfc1b90e1d8b703407bba6/barcode70.json barcode67.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/23/b5c28fa34510153a49e124979c20c1/barcode67.json barcode66.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/35/4aa2e83fd1b51dbd50b953d0ed59a4/barcode66.json barcode62.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/fd/9048bb5b2cffa7ec95482f51ac952f/barcode62.json barcode61.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/15/4f3566e4e2539055cea255f13d07f4/barcode61.json barcode60.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/13/cb4d0b361491ca273cae20465b6daa/barcode60.json barcode57.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/03/74ceda9c9d49cd1675ef922aecc2af/barcode57.json barcode56.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/03/81fcaaf736df8626501cce8e09f17d/barcode56.json barcode51.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/f1/4ec8e304ca588b1344b625bba9009c/barcode51.json barcode50.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/c6/3e33e1da0815594571b51973c4a79b/barcode50.json barcode47.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/c4/6ed7bc78b383aad6f65b790ac04b55/barcode47.json barcode46.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/1c/92450d07e308c1f391e9739386d566/barcode46.json barcode41.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/1d/9b33fa86ec50c78ac737fde362a3d1/barcode41.json barcode40.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/1c/008344ec69632ca9cef084453551eb/barcode40.json barcode37.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/9f/77faf44b43053827de11490d922458/barcode37.json barcode36.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/fd/95f5fbab00748048c873217646777e/barcode36.json barcode35.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/94/316d8b31bfaa1728ad12ca0b133d0c/barcode35.json barcode31.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/b1/5f580a8bf1fa2facff28b7ba4ac3d3/barcode31.json barcode30.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/48/56dcf55f01d32ef5c0d0f289c61414/barcode30.json barcode27.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/57/d5ac254a439dff833fc5829d56012a/barcode27.json barcode26.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/a6/7eae0e2215e3fff28c91bb22e1f87c/barcode26.json barcode23.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/ec/20f985fbe1800c24a1491bca68fe8e/barcode23.json barcode21.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/26/0ce04b1ef19e87767bf1c42b673ca0/barcode21.json barcode20.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/86/c591458aafc31cb1b44f32fbd7b921/barcode20.json barcode19.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/d7/16de22801cc813a18d5bf5d680b77a/barcode19.json barcode17.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/17/3b23681fbbaf2be8fc7b0d315bef0b/barcode17.json barcode16.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/24/5bdff7663b09c2a61e90c5b7f6014d/barcode16.json barcode11.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/83/17c4544b0b5fc6d22a785cb34269c3/barcode11.json barcode10.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/df/d54fced364361daea4b88f7cc0fc08/barcode10.json barcode06.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/9d/dba732dd965c6ab9bedfc4268be0e8/barcode06.json barcode01.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/34/ef85323b2264f0cd197e44a185bfc4/barcode01.json barcode07.json -> /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/work/76/3458ea0f4fae5d1d6451b08945a07d/barcode07.json
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I had the same error. I just removed --threads from input and process started, but it's very interesting - working all threads - Miracles!
Hi @EsbergA ! Please let me know if that solves the problem. However, it shouldn't break like that if just some barcodes are empty, so something else may be happening.
Hi @marsfro ! Thank you for using the workflow! Would you mind to send the output of the error, also are you running the workflow in the app or in the command line? In the last case, could you copy the command that you're using (also, to clarify what do you mean by removing 'threads' from the input)?
Removing all folders with only one tar.gz file solved the issue. This correlated to empty or very few reads assigned to the specific barcode. Thank you for the guidance
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Från: Natalia Garcia Garcia @.> Skickat: Tuesday, September 26, 2023 10:34:31 AM Till: epi2me-labs/wf-metagenomics @.> Kopia: Anders Esberg @.>; Mention @.> Ämne: Re: [epi2me-labs/wf-metagenomics] wf-metagenomic: Minimap error 16S_18S NCBI database (Issue #64)
Hi @EsbergAhttps://github.com/EsbergA ! Please let me know if that solves the problem. However, it shouldn't break like that if just some barcodes are empty, so something else may be happening.
Hi @marsfrohttps://github.com/marsfro ! Thank you for using the workflow! Would you mind to send the output of the error, also are you running the workflow in the app or in the command line? In the last case, could you copy the command that you're using (also, to clarify what do you mean by removing 'threads' from the input)?
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@nggvs Thank you for your pipeline!! I'm already tired of rushing between kraken2 and centrifuge
nextflow run epi2me-labs/wf-metagenomics --fastq /path/fastq_folders --classifier minimap2 --threads 8 - it was first run
nextflow run epi2me-labs/wf-metagenomics --fastq /path/fastq_folders --classifier minimap2 - second, without threads
nextflow_2nd_log.txt
nextflow_1st_log.txt
Caused by:
Process minimap_pipeline:minimap (12)
terminated with an error exit status (1)
Command executed:
minimap2 -t "8" -ax map-ont "ncbi_targeted_loci_16s_18s.fna" "seqs.fastq.gz" | samtools view -h -F 2304 - | workflow-glue format_minimap2 - -o "9o.minimap2.assignments.tsv" -r "ref2taxid.targloci.tsv" | samtools sort -o "9o.bam" -
samtools index "9o.bam"
awk -F '\t' '{print $3}' "9o.minimap2.assignments.tsv" > taxids.tmp
taxonkit --data-dir "taxonomy_dir" lineage -R taxids.tmp | workflow-glue aggregate_lineages -p "9o.minimap2"
file1=cat *.json
echo "{"'"9o"'": "$file1"}" >> temp
cp "temp" "9o.json"
Command exit status: 1
Command output: (empty)
Command error: [ERROR] failed to open file 'ncbi_targeted_loci_16s_18s.fna': No such file or directory [main_samview] fail to read the header from "-".
Hi @EsbergA !
Thank you for your comment. I guess it is a typo but just in case why do you mean by tar.gz files? The workflow takes fastq or gzip files, but not tar.gz. Besides, if you consider that your issue has been solved, please consider closing the issue and feel free to open a new one if you need it !
Hi @marsfro !
Thank you for using the pipeline! It seems that it is a different error. Could you open a new issue with it (it is useful to have different problems in different issues so that anyone in the future can check them, a way to organise them). The error that you have posted is from the first command using --threads
?
Operating System
macOS
Other Linux
No response
Workflow Version
wf-metagenomic v2.5.0
Workflow Execution
EPI2ME Desktop application
EPI2ME Version
No response
CLI command run
{ classifier: minimap2, batch_size: 0, analyse_unclassified: false, watch_path: false, database_set: ncbi_16s_18s, store_dir: store_dir, taxonomic_rank: S, port: 8080, host: localhost, external_kraken2: false, kraken2_memory_mapping: false, include_kraken2_assignments: false, kraken2_confidence: 0, minimap2exclude: false, split_prefix: false, keep_bam: false, amr: false, amr_db: resfinder, amr_minid: 80, amr_mincov: 80, abundance_threshold: 0, n_taxa_barplot: 8, out_dir: /Users/andersesberg/epi2melabs/instances/wf-metagenomics_01HAVTWSBWFR1X79WYJS52TPA3/output, min_len: 1200, threads: 4, server_threads: 4, kraken_clients: 2, disable_ping: false, help: false, version: false, fastq: /Users/andersesberg/epi2melabs/demo/epi2me-labs/wf-metagenomics/wf-metagenomics-demo/Lime9, min_read_qual: 12, max_len: 1700, wf: { agent: epi2melabs/5.1.2, epi2me_instance: 01HAVTWSBWFR1X79WYJS52TPA3, epi2me_user: guest_01HA9A2WRWMYV0YYDJJ20STRSX } }
Workflow Execution - CLI Execution Profile
standard (default)
What happened?
Run stopped with error in the minimap report step, all other step worked This happens with 2 out of 9 run. Tried to re-run but this does not help. Best regards Anders
Relevant log output
Application activity log entry
No response