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epi2me-labs / wf-pore-c

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demo data request #84

Open biozzq opened 2 weeks ago

biozzq commented 2 weeks ago

Hi，

Could you please let me know if there are any available raw signal data (.pod5) that I could use for practice? I am eager to see how well the process handles this issue (https://github.com/nanoporetech/modkit/issues/289), and having access to such data would be immensely helpful.

Thank you very much for your assistance!

Best regards, Zheng zhuqing

sarahjeeeze commented 2 weeks ago

Hi sure, there are various datasets available open source here - https://labs.epi2me.io/lc2024_t2t/

biozzq commented 1 week ago

Dear @sarahjeeeze

Thank you. I found the Pore-C base calling BAM file (PAW44788.bam, size 88G) on the website you shared. However, I am looking for a smaller Pod5 file that I can use just for testing. I would like to start from base calling and complete the whole processing workflow (assess the methylation information for Pore-C data).

Additionally, if you could provide a reference result file to use for comparison, it would be really helpful for checking the accuracy of my workflow.

Thank you very much for your help!

Best wishes, zheng zhuqing