Closed SamanthaStewart closed 10 months ago
Hi, Thanks for this feedback. I confirm that I also see a discrepancy when I run the workflow, we will investigate this and get back to you.
Hi Sarahjeeeze, have you already found a possible issue?
Hi, thanks for the reminder. Yes I think I found the issue, it's how the data is sorted in the report, will add a fix very soon - in the meantime it is the ...read_aln_stats.tsv files which have the correct values.
Hi, we added a fix for this in the latest version.
Closing, as should be resolved and no response for a while.
What happened?
When running the pipeline after multiplexing my RNA samples via the SQK-PCB109 protocol, the transcriptomes report in the end shows inconsistently assigned e.g. total read values compared to the single output files.
When comparing the Output files "..._read_aln_stats.tsv" I get the same values but they are assigned differently.
e.g. sample 12_X shows 271880 total reads (Report) And the Output file sample 12_X shows 384585 total reads
12_G shows 265334 total reads (Report) And the Output file sample 12_G shows 222981 total reads
The Outputfile of sample D_X of the output tsv file suits the numbers of the total reads of G_G of the Report.
It looks like the report isn’t sorting the sample ID correctly. The numbers are found 1:1. They’re just not assigned to the same samples.
Which assignment can be trusted now? The report or the Outputs of e.g., "..._read_aln_stats.tsv"?
I already checked my sample and condition sheets. I ran the pipeline when sorted by barcodes, sorted by sampleIDs, and made sure that both sheets were sorted the same way. The troubleshooting did not change the results. The inconsistencies still occurred.![issue](https://github.com/epi2me-labs/wf-transcriptomes/assets/133666247/aec35615-10de-476a-a718-02cecc6834d1)
What´s the problem here? How can I solve it?
Operating System
Windows 10
Workflow Execution
EPI2ME Labs desktop application
Workflow Execution - EPI2ME Labs Versions
version 22.04.5
Workflow Execution - CLI Execution Profile
None
Workflow Version
transcriptomes v0.1.8
Relevant log output