Closed jennyujin22 closed 2 months ago
Dear Yujin,
Can you share your sample annotation sheet and the output of ls in your idat directory? Also please show me the command that you use to start RnBeads. You can send this by email to michael.scherer at dkfz.de
Best,
Michael
jennyujin22 @.***> schrieb am Do., 9. Mai 2024, 09:22:
Dear Michael,
Even I already specify the barcode (SentrixID_SentrixPosition) and check if IDAT prefix is corresponding to barcode, there is an error "Some IDAT files are not present in the supplied base directory". (also I checked scientific notations).
here is my sessionInfo()
R version 4.4.0 (2024-04-24 ucrt) Platform: x86_64-w64-mingw32/x64 Running under: Windows 11 x64 (build 22631)
Matrix products: default
locale: [1] LC_COLLATE=Korean_Korea.utf8 LC_CTYPE=Korean_Korea.utf8 LC_MONETARY=Korean_Korea.utf8 LC_NUMERIC=C [5] LC_TIME=Korean_Korea.utf8
time zone: Asia/Seoul tzcode source: internal
attached base packages: [1] parallel grid stats4 stats graphics grDevices utils datasets methods base
other attached packages: [1] RnBeads.hg19_1.36.0 RnBeads_2.22.0 plyr_1.8.9 [4] methylumi_2.50.0 minfi_1.50.0 bumphunter_1.46.0 [7] locfit_1.5-9.9 iterators_1.0.14 foreach_1.5.2 [10] Biostrings_2.72.0 XVector_0.44.0 SummarizedExperiment_1.34.0 [13] MatrixGenerics_1.16.0 FDb.InfiniumMethylation.hg19_2.2.0 org.Hs.eg.db_3.19.1 [16] TxDb.Hsapiens.UCSC.hg19.knownGene_3.2.2 GenomicFeatures_1.56.0 AnnotationDbi_1.66.0 [19] reshape2_1.4.4 scales_1.3.0 Biobase_2.64.0 [22] illuminaio_0.46.0 matrixStats_1.3.0 limma_3.60.0 [25] gridExtra_2.3 gplots_3.1.3.1 ggplot2_3.5.1 [28] fields_15.2 viridisLite_0.4.2 spam_2.10-0 [31] ff_4.0.12 bit_4.0.5 cluster_2.1.6 [34] MASS_7.3-60.2 GenomicRanges_1.56.0 GenomeInfoDb_1.40.0 [37] IRanges_2.38.0 S4Vectors_0.41.6 sesame_1.22.0 [40] sesameData_1.22.0 ExperimentHub_2.12.0 AnnotationHub_3.12.0 [43] BiocFileCache_2.11.2 dbplyr_2.5.0 BiocGenerics_0.49.1
loaded via a namespace (and not attached): [1] RColorBrewer_1.1-3 rstudioapi_0.16.0 jsonlite_1.8.8 magrittr_2.0.3 BiocIO_1.14.0 [6] zlibbioc_1.50.0 vctrs_0.6.5 multtest_2.60.0 memoise_2.0.1 Rsamtools_2.20.0 [11] DelayedMatrixStats_1.26.0 RCurl_1.98-1.14 askpass_1.2.0 S4Arrays_1.4.0 curl_5.2.1 [16] Rhdf5lib_1.26.0 rhdf5_2.48.0 SparseArray_1.4.0 nor1mix_1.3-3 KernSmooth_2.23-22 [21] cachem_1.0.8 GenomicAlignments_1.40.0 lifecycle_1.0.4 pkgconfig_2.0.3 Matrix_1.7-0 [26] R6_2.5.1 fastmap_1.1.1 GenomeInfoDbData_1.2.12 digest_0.6.35 siggenes_1.78.0 [31] reshape_0.8.9 colorspace_2.1-0 RSQLite_2.3.6 base64_2.0.1 filelock_1.0.3 [36] fansi_1.0.6 beanplot_1.3.1 httr_1.4.7 abind_1.4-5 compiler_4.4.0 [41] rngtools_1.5.2 bit64_4.0.5 withr_3.0.0 BiocParallel_1.38.0 DBI_1.2.2 [46] HDF5Array_1.32.0 maps_3.4.2 openssl_2.1.2 rappdirs_0.3.3 DelayedArray_0.30.0 [51] rjson_0.2.21 gtools_3.9.5 caTools_1.18.2 tools_4.4.0 quadprog_1.5-8 [56] glue_1.7.0 restfulr_0.0.15 nlme_3.1-164 rhdf5filters_1.16.0 generics_0.1.3 [61] gtable_0.3.5 tzdb_0.4.0 preprocessCore_1.66.0 tidyr_1.3.1 data.table_1.15.4 [66] hms_1.1.3 xml2_1.3.6 utf8_1.2.4 BiocVersion_3.19.1 pillar_1.9.0 [71] stringr_1.5.1 genefilter_1.86.0 splines_4.4.0 dplyr_1.1.4 lattice_0.22-6 [76] survival_3.5-8 rtracklayer_1.64.0 GEOquery_2.72.0 annotate_1.82.0 tidyselect_1.2.1 [81] wheatmap_0.2.0 scrime_1.3.5 statmod_1.5.0 stringi_1.8.3 UCSC.utils_1.0.0 [86] yaml_2.3.8 codetools_0.2-20 tibble_3.2.1 BiocManager_1.30.23 cli_3.6.2 [91] xtable_1.8-4 munsell_0.5.1 Rcpp_1.0.12 png_0.1-8 XML_3.99-0.16.1 [96] readr_2.1.5 blob_1.2.4 mclust_6.1.1 dotCall64_1.1-1 doRNG_1.8.6 [101] sparseMatrixStats_1.16.0 bitops_1.0-7 purrr_1.0.2 crayon_1.5.2 rlang_1.1.3 [106] KEGGREST_1.44.0
Thanks a lot.
Best,
Yujin
Originally posted by @jennyujin22 https://github.com/jennyujin22 in #38 (comment) https://github.com/epigen/RnBeads/issues/38#issuecomment-2102102765
— Reply to this email directly, view it on GitHub https://github.com/epigen/RnBeads/issues/54, or unsubscribe https://github.com/notifications/unsubscribe-auth/ADYQQ6CXHQNHA2PIQUUIVBTZBMP4XAVCNFSM6AAAAABHOKAWUKVHI2DSMVQWIX3LMV43ASLTON2WKOZSGI4DOMBWHAZDGMA . You are receiving this because you are subscribed to this thread.Message ID: @.***>
I sent it by email :)
Thanks a lot!
Dear Michael,
Even I already specify the barcode (SentrixID_SentrixPosition) and check if IDAT prefix is corresponding to barcode, there is an error "Some IDAT files are not present in the supplied base directory". (also I checked scientific notations).
here is my sessionInfo()
R version 4.4.0 (2024-04-24 ucrt) Platform: x86_64-w64-mingw32/x64 Running under: Windows 11 x64 (build 22631)
Matrix products: default
locale: [1] LC_COLLATE=Korean_Korea.utf8 LC_CTYPE=Korean_Korea.utf8 LC_MONETARY=Korean_Korea.utf8 LC_NUMERIC=C
[5] LC_TIME=Korean_Korea.utf8
time zone: Asia/Seoul tzcode source: internal
attached base packages: [1] parallel grid stats4 stats graphics grDevices utils datasets methods base
other attached packages: [1] RnBeads.hg19_1.36.0 RnBeads_2.22.0 plyr_1.8.9
[4] methylumi_2.50.0 minfi_1.50.0 bumphunter_1.46.0
[7] locfit_1.5-9.9 iterators_1.0.14 foreach_1.5.2
[10] Biostrings_2.72.0 XVector_0.44.0 SummarizedExperiment_1.34.0
[13] MatrixGenerics_1.16.0 FDb.InfiniumMethylation.hg19_2.2.0 org.Hs.eg.db_3.19.1
[16] TxDb.Hsapiens.UCSC.hg19.knownGene_3.2.2 GenomicFeatures_1.56.0 AnnotationDbi_1.66.0
[19] reshape2_1.4.4 scales_1.3.0 Biobase_2.64.0
[22] illuminaio_0.46.0 matrixStats_1.3.0 limma_3.60.0
[25] gridExtra_2.3 gplots_3.1.3.1 ggplot2_3.5.1
[28] fields_15.2 viridisLite_0.4.2 spam_2.10-0
[31] ff_4.0.12 bit_4.0.5 cluster_2.1.6
[34] MASS_7.3-60.2 GenomicRanges_1.56.0 GenomeInfoDb_1.40.0
[37] IRanges_2.38.0 S4Vectors_0.41.6 sesame_1.22.0
[40] sesameData_1.22.0 ExperimentHub_2.12.0 AnnotationHub_3.12.0
[43] BiocFileCache_2.11.2 dbplyr_2.5.0 BiocGenerics_0.49.1
loaded via a namespace (and not attached): [1] RColorBrewer_1.1-3 rstudioapi_0.16.0 jsonlite_1.8.8 magrittr_2.0.3 BiocIO_1.14.0
[6] zlibbioc_1.50.0 vctrs_0.6.5 multtest_2.60.0 memoise_2.0.1 Rsamtools_2.20.0
[11] DelayedMatrixStats_1.26.0 RCurl_1.98-1.14 askpass_1.2.0 S4Arrays_1.4.0 curl_5.2.1
[16] Rhdf5lib_1.26.0 rhdf5_2.48.0 SparseArray_1.4.0 nor1mix_1.3-3 KernSmooth_2.23-22
[21] cachem_1.0.8 GenomicAlignments_1.40.0 lifecycle_1.0.4 pkgconfig_2.0.3 Matrix_1.7-0
[26] R6_2.5.1 fastmap_1.1.1 GenomeInfoDbData_1.2.12 digest_0.6.35 siggenes_1.78.0
[31] reshape_0.8.9 colorspace_2.1-0 RSQLite_2.3.6 base64_2.0.1 filelock_1.0.3
[36] fansi_1.0.6 beanplot_1.3.1 httr_1.4.7 abind_1.4-5 compiler_4.4.0
[41] rngtools_1.5.2 bit64_4.0.5 withr_3.0.0 BiocParallel_1.38.0 DBI_1.2.2
[46] HDF5Array_1.32.0 maps_3.4.2 openssl_2.1.2 rappdirs_0.3.3 DelayedArray_0.30.0
[51] rjson_0.2.21 gtools_3.9.5 caTools_1.18.2 tools_4.4.0 quadprog_1.5-8
[56] glue_1.7.0 restfulr_0.0.15 nlme_3.1-164 rhdf5filters_1.16.0 generics_0.1.3
[61] gtable_0.3.5 tzdb_0.4.0 preprocessCore_1.66.0 tidyr_1.3.1 data.table_1.15.4
[66] hms_1.1.3 xml2_1.3.6 utf8_1.2.4 BiocVersion_3.19.1 pillar_1.9.0
[71] stringr_1.5.1 genefilter_1.86.0 splines_4.4.0 dplyr_1.1.4 lattice_0.22-6
[76] survival_3.5-8 rtracklayer_1.64.0 GEOquery_2.72.0 annotate_1.82.0 tidyselect_1.2.1
[81] wheatmap_0.2.0 scrime_1.3.5 statmod_1.5.0 stringi_1.8.3 UCSC.utils_1.0.0
[86] yaml_2.3.8 codetools_0.2-20 tibble_3.2.1 BiocManager_1.30.23 cli_3.6.2
[91] xtable_1.8-4 munsell_0.5.1 Rcpp_1.0.12 png_0.1-8 XML_3.99-0.16.1
[96] readr_2.1.5 blob_1.2.4 mclust_6.1.1 dotCall64_1.1-1 doRNG_1.8.6
[101] sparseMatrixStats_1.16.0 bitops_1.0-7 purrr_1.0.2 crayon_1.5.2 rlang_1.1.3
[106] KEGGREST_1.44.0
Thanks a lot.
Best,
Yujin
Originally posted by @jennyujin22 in https://github.com/epigen/RnBeads/issues/38#issuecomment-2102102765