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epigen / crop-seq

Data analysis scripts for Datlinger et. al, 2017 (doi:10.1038/nmeth.4177)
https://crop-seq.computational-epigenetics.org
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Issue Running assign_gRNA_cells.py #3

Closed KASHISH72 closed 4 years ago

KASHISH72 commented 6 years ago

Hey I am trying to run the whole pipeline for crop-seq, i am at the assign_gRNA_cells.py step and it is giving error. File "assign_gRNA_cells.py", line 359, in prj.add_sample_sheet() File "crop-seq-final_version/src/looper/looper/models.py", line 546, in getattr AttributeError: add_sample_sheet

Please let me know if you have any idea about the error

Thanks, Kashish Chetal

afrendeiro commented 6 years ago

Hi, and thanks for the interest. This project was run with an older version of looper (prior to the 0.4 release). Since then add_sample_sheet is called by the __init__ method when initializing a new Project. You can therefore simply skip that line if you use for example looper >=0.6.

KASHISH72 commented 6 years ago

Hello Thanks for the quick response but it is still giving the error.

Traceback (most recent call last): File "src/assign_gRNA_cells.py", line 359, in prj.paths.results_dir = results_dir = os.path.join("results") File "crop-seq-final_version/src/looper/looper/models.py", line 546, in getattr AttributeError: paths

afrendeiro commented 6 years ago

That is a different error. That line to is also not compatible with newer looper versions. Those variables are in fact not used in the entire script, so please also skip it.

KASHISH72 commented 6 years ago

Now it is giving a different error

Traceback (most recent call last): File "src/assign_gRNA_cells.py", line 373, in reads = get_reads_in_construct(bam, sel_guide_annotation) File "src/assign_gRNA_cells.py", line 37, in get_reads_in_construct chrom_size = len(prj.config['crop-seq']['u6'] + guide_seq + prj.config['crop-seq']['rest']) File "crop-seq-final_version/src/looper/looper/models.py", line 546, in getattr AttributeError: config

afrendeiro commented 6 years ago

Alright, sorry. In newer looper versions the config attribute was merged into the Project object directly. Please see commit 7b275271eeb88a7017a248000db967e0da98340a with these instances updated.

KASHISH72 commented 6 years ago

Hello Andre,

I made the changes you suggested and the script is outputing this.

DNMT3B_1 Traceback (most recent call last): File "src/assign_gRNA_cells.py", line 373, in reads = get_reads_in_construct(bam, sel_guide_annotation) File "src/assign_gRNA_cells.py", line 42, in get_reads_in_construct for aln in bam_handle.fetch(reference=chrom + "_chrom"): File "pysam/calignmentfile.pyx", line 897, in pysam.calignmentfile.AlignmentFile.fetch (pysam/calignmentfile.c:10997) File "pysam/calignmentfile.pyx", line 819, in pysam.calignmentfile.AlignmentFile.parse_region (pysam/calignmentfile.c:10455)

On Tue, Jan 2, 2018 at 2:28 PM, André F. Rendeiro notifications@github.com wrote:

Alright, sorry. In newer looper versions the config attribute was merged into the Project object directly. Please see commit 7b27527 https://github.com/epigen/crop-seq/commit/7b275271eeb88a7017a248000db967e0da98340a with these instances updated.

— You are receiving this because you authored the thread. Reply to this email directly, view it on GitHub https://github.com/epigen/crop-seq/issues/3#issuecomment-354854226, or mute the thread https://github.com/notifications/unsubscribe-auth/AGe7zelkTZfsy93oYeYZ7C9HbG_Wi058ks5tGoNQgaJpZM4RQ5J3 .

nsheff commented 6 years ago

Sorry for these annoyances. This stuff is stabilizing, though, so these should become less of an issue going forward :)

afrendeiro commented 6 years ago

Hi Kashish, I'm not sure about what is happening. Is the error message complete? I can't debug right now since I do not have access to the files, will have to have a look next week.

KASHISH72 commented 6 years ago

Sure Andre Here is the error message again.

DNMT3B_1 Traceback (most recent call last): File "src/assign_gRNA_cells.py", line 373, in reads = get_reads_in_construct(bam, sel_guide_annotation) File "src/assign_gRNA_cells.py", line 42, in get_reads_in_construct for aln in bam_handle.fetch(reference=chrom + "_chrom"): File "pysam/calignmentfile.pyx", line 897, in pysam.calignmentfile.AlignmentFile.fetch (pysam/calignmentfile.c:10997) File "pysam/calignmentfile.pyx", line 819, in pysam.calignmentfile.AlignmentFile.parse_region (pysam/calignmentfile.c:10455) ValueError: invalid reference DNMT3B_1_chrom

On Tue, Jan 2, 2018 at 3:14 PM, André F. Rendeiro notifications@github.com wrote:

Hi Kashish, I'm not sure about what is happening. Is the error message complete? I can't debug right now since I do not have access to the files, will have to have a look next week.

— You are receiving this because you authored the thread. Reply to this email directly, view it on GitHub https://github.com/epigen/crop-seq/issues/3#issuecomment-354864386, or mute the thread https://github.com/notifications/unsubscribe-auth/AGe7zQaFUc3bhe6yh5PEUZnXm56ajVGfks5tGo4egaJpZM4RQ5J3 .

afrendeiro commented 6 years ago

Ah, okay. It seems that your BAM file does not contain "DNMT3B_1_chrom" as a reference (chromosome). Did you map your reads to a reference containing the artificial gRNA chromosomes as described here? To confirm, see if these references are in the header of the BAM file.

KASHISH72 commented 6 years ago

Hello Andre,

I downloaded the reference genome from your crop-seq website ( http://www.medical-epigenomics.org/papers/datlinger2017/data/genome_refs/hg38_spiked_Tcrlibrary.tar.gz ) http://www.medical-epigenomics.org/papers/datlinger2017/. And I did check in the bam file "DNMT3B_1_chrom" not present here. I ran the whole protocol described till the command : looper runmetadat/cnfig.yaml

Thanks, Kashish Chetal

On Tue, Jan 2, 2018 at 3:26 PM, André F. Rendeiro notifications@github.com wrote:

Ah, okay. It seems that your BAM file does not contain "DNMT3B_1_chrom" as a reference (chromosome). Did you map your reads to a reference containing the artificial gRNA chromosomes as described here https://github.com/epigen/crop-seq/blob/master/src/guides_to_ref.py? To confirm, see if these references are in the header of the BAM file.

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afrendeiro commented 6 years ago

So "DNMT3B_1_chrom" is not present in the refence genome or in the BAM file? I assume you also ran that command to process the data with the Drop-seq pipeline or else you wouldn't have the mapped BAM files yet. Are you trying to run the same experiments as published or some new experiments? If the same, the mapped BAM files should definitely contain reads in all of the gRNA chromosomes. Please check the pipeline log files for errors or warnings that show that the pipeline completed successfully and there aren't for example truncated files for some reason.

KASHISH72 commented 6 years ago

So "DNMT3B_1_chrom" is not present in BAM file. Yes I ran the Drop-Seq pipeline and I have the mapped BAM file but i am only running it for one sample i.e CROP-seq_HEK293T. Here I have also attached the drop-seq pipeline log file which has the information for all of the steps in drop-seq pipeline. And you can see there is not any error in the log file.

On Tue, Jan 2, 2018 at 3:44 PM, André F. Rendeiro notifications@github.com wrote:

So "DNMT3B_1_chrom" is not present in the refence genome or in the BAM file? I assume you also ran that command to process the data with the Drop-seq pipeline or else you wouldn't have the mapped BAM files yet. Are you trying to run the same experiments as published or some new experiments? If the same, the mapped BAM files should definitely contain reads in all of the gRNA chromosomes. Please check the pipeline log files for errors or warnings that show that the pipeline completed successfully and there aren't for example truncated files for some reason.

— You are receiving this because you authored the thread. Reply to this email directly, view it on GitHub https://github.com/epigen/crop-seq/issues/3#issuecomment-354870507, or mute the thread https://github.com/notifications/unsubscribe-auth/AGe7zYeM-TGW8qpkelCNJvJyVrbHWzHkks5tGpUUgaJpZM4RQ5J3 .

afrendeiro commented 6 years ago

Hmm that's odd then (sorry attachments don't work in the github issue tracker). Is it only this particular gRNA chromosome missing or the others too? For that experiment in particular there should be reads from 4 different gRNAs (annotated here) I believe.

KASHISH72 commented 6 years ago

Here is the header of the bam file.

@HD VN:1.5 SO:coordinate @SQ SN:1 LN:248956422 M5:8bffd97bbd14d38b00301bf24ce91d66 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:10 LN:133797422 M5:98ce4a08c55669254221343c4e6d900f AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:11 LN:135086622 M5:adcd2eb7d2bfd326ea794c3016deda42 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:12 LN:133275309 M5:5f431c32ee15f6b9aa60ef685c36837a AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:13 LN:114364328 M5:1992152ef5eddf896889020f667a560a AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:14 LN:107043718 M5:344ffbe05c38c5deebefcef1e3075745 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:15 LN:101991189 M5:ee51939687fd1d54e5bb5945e20ba261 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:16 LN:90338345 M5:8ca3a25e95d982958c9c924a44f69dc9 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:17 LN:83257441 M5:0c913eca0538224e70671418b7803b10 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:18 LN:80373285 M5:86aec8dec2329a0342bdf85dc472bc4f AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:19 LN:58617616 M5:c59a4a40b1239fdd3e0be6fca0a43c5b AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:2 LN:242193529 M5:64e6572ed77a93a1b7765bc8c69b918d AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:20 LN:64444167 M5:138e2ed5da8650c8bfadf83135cecd18 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:21 LN:46709983 M5:897a4bfbe161b92fb59bfb68aa7fae04 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:22 LN:50818468 M5:5f30490bd612c6d85a3d9fc207064b10 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:3 LN:198295559 M5:d62aa223e682e31751f8e6ee50336104 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:4 LN:190214555 M5:e407b27f2e8c791cea1a9252dbaed658 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:5 LN:181538259 M5:52f18bb8485a2797c3af6fff2046d6a7 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:6 LN:170805979 M5:62809fbd6f2aab4c2411868a7457da1e AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:7 LN:159345973 M5:ae130566bf9ebdc08204a50239269ad8 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:8 LN:145138636 M5:7bd523a23148928a9c59cadcf5df19d3 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:9 LN:138394717 M5:d8bc0fa62cbc00ae64568194a18520f2 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:MT LN:16569 M5:c68f52674c9fb33aef52dcf399755519 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:X LN:156040895 M5:38bf575987f65476f79e937f7d322733 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Y LN:57227415 M5:a74384b1e68246922fc0bbf00c932a0a AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270728.1 LN:1872759 M5:369ff74cf36683b3066a2ca929d9c40d AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270727.1 LN:448248 M5:84fe18a7bf03f3b7fc76cbac8eb583f1 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270442.1 LN:392061 M5:796289c4cda40e358991f9e672490015 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270729.1 LN:280839 M5:2756f6ee4f5780acce31e995443508b6 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:GL000225.1 LN:211173 M5:63945c3e6962f28ffd469719a747e73c AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270743.1 LN:210658 M5:3b62d9d3100f530d509e4efebd98502c AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:GL000008.2 LN:209709 M5:a999388c587908f80406444cebe80ba3 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:GL000009.2 LN:201709 M5:862f555045546733591ff7ab15bcecbe AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270747.1 LN:198735 M5:62375d812ece679c9fd2f3d08d4e22a4 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270722.1 LN:194050 M5:51f46c9093929e6edc3b4dfd50d803fc AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:GL000194.1 LN:191469 M5:6ac8f815bf8e845bb3031b73f812c012 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270742.1 LN:186739 M5:2f31c013a4a8301deb8ab7ed1ca1cd99 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:GL000205.2 LN:185591 M5:458e71cd53dd1df4083dc7983a6c82c4 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:GL000195.1 LN:182896 M5:5d9ec007868d517e73543b005ba48535 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270736.1 LN:181920 M5:2ff189f33cfa52f321accddf648c5616 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270733.1 LN:179772 M5:f1fa05d48bb0c1f87237a28b66f0be0b AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:GL000224.1 LN:179693 M5:d5b2fc04f6b41b212a4198a07f450e20 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:GL000219.1 LN:179198 M5:f977edd13bac459cb2ed4a5457dba1b3 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270719.1 LN:176845 M5:cd5e932cfc4c74d05bb64e2126873a3a AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:GL000216.2 LN:176608 M5:725009a7e3f5b78752b68afa922c090c AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270712.1 LN:176043 M5:c38a0fecae6a1838a405406f724d6838 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270706.1 LN:175055 M5:62def1a794b3e18192863d187af956e6 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270725.1 LN:172810 M5:edc6402e58396b90b8738a5e37bf773d AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270744.1 LN:168472 M5:e90aee46b947ff8c32291a6843fde3f9 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270734.1 LN:165050 M5:1d17410ae2569c758e6dd51616412d32 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:GL000213.1 LN:164239 M5:9d424fdcc98866650b58f004080a992a AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:GL000220.1 LN:161802 M5:fc35de963c57bf7648429e6454f1c9db AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270715.1 LN:161471 M5:b65a8af1d7bbb7f3c77eea85423452bb AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:GL000218.1 LN:161147 M5:1d708b54644c26c7e01c2dad5426d38c AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270749.1 LN:158759 M5:c899a7b4e911d371283f3f4058ca08b7 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270741.1 LN:157432 M5:86eaea8a15a3950e37442eaaa5c9dc92 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:GL000221.1 LN:155397 M5:3238fb74ea87ae857f9c7508d315babb AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270716.1 LN:153799 M5:2828e63b8edc5e845bf48e75fbad2926 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270731.1 LN:150754 M5:8176d9a20401e8d9f01b7ca8b51d9c08 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270751.1 LN:150742 M5:1b758bbdee0e9ca882058d916cba9d29 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270750.1 LN:148850 M5:c022ba92f244b7dc54ea90c4eef4d554 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270519.1 LN:138126 M5:8d754e9c9afd904fba0a2cd577fcc9a1 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:GL000214.1 LN:137718 M5:46c2032c37f2ed899eb41c0473319a69 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270708.1 LN:127682 M5:1e95e047b98ed92148dd84d6c037158c AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270730.1 LN:112551 M5:48f98ede8e28a06d241ab2e946c15e07 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270438.1 LN:112505 M5:e765271939b854dd6826aa764e930c87 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270737.1 LN:103838 M5:2ea8bc113a8193d1d700b584b2c5f42a AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270721.1 LN:100316 M5:9654b5d3f36845bb9d19a6dbd15d2f22 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270738.1 LN:99375 M5:854ec525c7b6a79e7268f515b6a9877c AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270748.1 LN:93321 M5:4f6c6ab005c852a4352aa33e7cc88ded AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270435.1 LN:92983 M5:1655c75415b9c29e143a815f44286d05 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:GL000208.1 LN:92689 M5:aa81be49bf3fe63a79bdc6a6f279abf6 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270538.1 LN:91309 M5:d60b72221cc7af871f2c757577e4c92a AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270756.1 LN:79590 M5:2996b120a5a5e15dab6555f0bf92e374 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270739.1 LN:73985 M5:760fbd73515fedcc9f37737c4a722d6a AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270757.1 LN:71251 M5:174c73b60b41d8a1ef0fbaa4b3bdf0d3 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270709.1 LN:66860 M5:4e2db2933ea96aee8dab54af60ecb37d AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270746.1 LN:66486 M5:c470486a0a858e14aa21d7866f83cc17 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270753.1 LN:62944 M5:25075fb2a1ecada67c0eb2f1fe0c7ec9 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270589.1 LN:44474 M5:211c215414693fe0a2399cf82e707e03 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270726.1 LN:43739 M5:fbe54a3197e2b469ccb2f4b161cfbe86 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270735.1 LN:42811 M5:eb6b07b73dd9a47252098ed3d9fb78b8 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270711.1 LN:42210 M5:af383f98cf4492c1f1c4e750c26cbb40 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270745.1 LN:41891 M5:1386fe3de6f82956f2124e19353ff9c1 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270714.1 LN:41717 M5:42f7a452b8b769d051ad738ee9f00631 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270732.1 LN:41543 M5:d837bab5e416450df6e1038ae6cd0817 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270713.1 LN:40745 M5:cb78d48cc0adbc58822a1c6fe89e3569 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270754.1 LN:40191 M5:fe9e16233cecbc244f06f3acff3d03b8 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270710.1 LN:40176 M5:9949f776680c6214512ee738ac5da289 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270717.1 LN:40062 M5:796773a1ee67c988b4de887addbed9e7 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270724.1 LN:39555 M5:c3fcb15dddf45f91ef7d94e2623ce13b AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270720.1 LN:39050 M5:8c2683400a4aeeb40abff96652b9b127 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270723.1 LN:38115 M5:74a4b480675592095fb0c577c515b5df AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270718.1 LN:38054 M5:b0c463c8efa8d64442b48e936368dad5 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270317.1 LN:37690 M5:cd4b1fda800f6ec9ea8001994dbf6499 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270740.1 LN:37240 M5:69e42252aead509bf56f1ea6fda91405 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270755.1 LN:36723 M5:4a7da6a658955bd787af8add3ccb5751 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270707.1 LN:32032 M5:78135804eb15220565483b7cdd02f3be AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270579.1 LN:31033 M5:fe62fb1964002717cc1b034630e89b1f AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270752.1 LN:27745 M5:e0880631848337bd58559d9b1519da63 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270512.1 LN:22689 M5:ba1021c82d1230af856f59079e2f71b4 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270322.1 LN:21476 M5:7d459255d1c54369e3b64e719061a5a5 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:GL000226.1 LN:15008 M5:1c1b2cd1fccbc0a99b6a447fa24d1504 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270311.1 LN:12399 M5:59594f9012d8ce21ed5d1119c051a2ba AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270366.1 LN:8320 M5:44a0b65b7ba6bcff37eca202e7d966ea AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270511.1 LN:8127 M5:907ca34a4a2a6673632ebaf513a4c1a4 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270448.1 LN:7992 M5:0f40827c265cb813b6e723da6c9b926b AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270521.1 LN:7642 M5:af5bef7cefec7bd7efa729ac6c5be088 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270581.1 LN:7046 M5:9f26945f9f9b3f865c9ebe953cbbc1a9 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270582.1 LN:6504 M5:6fd9804a7478d2e28160fe9f017689cb AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270515.1 LN:6361 M5:dd7527ee8e0bdb0a43ca0b2a5456c8c3 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270588.1 LN:6158 M5:37ffa850e69b342a8f8979bd3ffc77d4 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270591.1 LN:5796 M5:d6af509d69835c9ac25a30086e5a4051 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270522.1 LN:5674 M5:070b4678e22501029c2e3297115216bc AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270507.1 LN:5353 M5:311894d0a815eb07c5cac49da851cb4a AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270590.1 LN:4685 M5:e8a57f147561b361091791b9010cd28b AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270584.1 LN:4513 M5:d93636c9d54abd013cfc0d4c01334032 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270320.1 LN:4416 M5:d898b9c5a0118e76481bf5695272959e AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270382.1 LN:4215 M5:e7085cdcee6ad62f359744e13d3209fc AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270468.1 LN:4055 M5:0a603090f06108ed7aff75df0767b822 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270467.1 LN:3920 M5:db34e0dc109a4afd499b5ec6aaae9754 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270362.1 LN:3530 M5:bc82401ffd9a5ae711fa0ea34da8d2f0 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270517.1 LN:3253 M5:913440c38d95c618617ca69bb9296170 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270593.1 LN:3041 M5:f4a5bfa203e9e81acb640b18fb11e78e AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270528.1 LN:2983 M5:d75c9235f0b8c449fc4352997c56b086 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270587.1 LN:2969 M5:36be47659719f47b95caa51744aa8f70 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270364.1 LN:2855 M5:6ff66a8e589ca27d93b5bac0e5b13a87 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270371.1 LN:2805 M5:a0af3d778dfeb7963e8e6d84c0c54fba AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270333.1 LN:2699 M5:57baf650c47bba9b3a8b7c6d0fb55ad6 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270374.1 LN:2656 M5:dbc92c9a92e558946e58b4909ec95dd5 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270411.1 LN:2646 M5:fc240322d91d43c04f349cc58fda3eca AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270414.1 LN:2489 M5:753e02ef3b1c590e0e3376ad2ebb5836 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270510.1 LN:2415 M5:cd7348b3b5d9d0dfef6aed2af75ce920 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270390.1 LN:2387 M5:7a64d89ea14990c16d20f4d6e7283e10 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270375.1 LN:2378 M5:d678250c97e9b94aa390fa46e70a6d83 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270420.1 LN:2321 M5:bac954a897539c91982a7e3985a49910 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270509.1 LN:2318 M5:1cdeb8c823d839e1d1735b5bc9a14856 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270315.1 LN:2276 M5:ecc43e822dc011fae1fcfd9981c46e9c AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270302.1 LN:2274 M5:ee6dff38036f7d03478c70717643196e AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270518.1 LN:2186 M5:3fd898b62ca859f50fb8b83e7706352b AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270530.1 LN:2168 M5:04549369e1197c626669a10164613635 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270304.1 LN:2165 M5:9423c1b46a48aa6331a77ab5c702ac9d AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270418.1 LN:2145 M5:1537ec12b9c58d137a2d4cb9db896bbc AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270424.1 LN:2140 M5:747c8f94f34d5de4ad289bc604708210 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270417.1 LN:2043 M5:cd26758fda713f9c96e51d541f50c2d0 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270508.1 LN:1951 M5:7d42a358d472b9cbdfdf30c8742473d0 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270303.1 LN:1942 M5:2cb746c78e0faa11e628603a4bc9bd58 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270381.1 LN:1930 M5:2a9297cfd3b3807195ab9ad07e775d99 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270529.1 LN:1899 M5:4caf890f2586daab8e4b2e2db904f05f AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270425.1 LN:1884 M5:665a46879bbb48294b0cfa87b61e71f6 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270396.1 LN:1880 M5:9069bed3c2efe7cc87227d619ad5816f AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270363.1 LN:1803 M5:6edd17a912f391022edbc192d49f2489 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270386.1 LN:1788 M5:b9b1baaa7abf206f6b70cf31654172db AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270465.1 LN:1774 M5:bb1b2323414425c46531b3c3d22ae00d AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270383.1 LN:1750 M5:694d75683e4a9554bcc1291edbcaee43 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270384.1 LN:1658 M5:b06e44ea15d0a57618d6ca7d2e6ac5d2 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270330.1 LN:1652 M5:c2c590706a339007b00c59e0b8937e78 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270372.1 LN:1650 M5:53a9d5e8fd28bce5da5efcfd9114dbf2 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270548.1 LN:1599 M5:866b0db8e9cf66208c2c064bd09ce0a2 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270580.1 LN:1553 M5:1df30dae0f605811d927dcea58e729fc AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270387.1 LN:1537 M5:22a12462264340c25e912b8485cdfa91 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270391.1 LN:1484 M5:1fa5cf03b3eac0f1b4a64948fd09de53 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270305.1 LN:1472 M5:f9acea3395b6992cf3418b6689b98cf9 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270373.1 LN:1451 M5:b174fe53be245a840cd6324e39b88ced AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270422.1 LN:1445 M5:3fce80eb4c0554376b591699031feb56 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270316.1 LN:1444 M5:6adde7a9fe7bd6918f12d0f0924aa8ba AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270340.1 LN:1428 M5:56b462bac20d385cdfcde0155fe4c3a1 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270338.1 LN:1428 M5:301ef75a6b2996d745eb3464bd352b57 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270583.1 LN:1400 M5:b127e2e6dbe358ff192b271b8c6ee690 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270334.1 LN:1368 M5:53afe12d1371f250a3d1de655345d374 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270429.1 LN:1361 M5:ee8962dbef9396884f649e566b78bf06 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270393.1 LN:1308 M5:3724e1d70677d6b5c4bcf17fd40da111 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270516.1 LN:1300 M5:1cbaaafbbf016906a5bf5886f5a0ecb7 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270389.1 LN:1298 M5:2c9b08c57c27e714d4d5259fd91b6983 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270466.1 LN:1233 M5:530b7033716a5d72dd544213c513fd12 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270388.1 LN:1216 M5:76f9f3315fa4b831e93c36cd88196480 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270544.1 LN:1202 M5:e62a14b14467cdf48b5a236c66918f0f AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270310.1 LN:1201 M5:af6cb123af7007793bac06485a2a20e9 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270412.1 LN:1179 M5:7bb9612f733fb7f098be79499d46350c AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270395.1 LN:1143 M5:7c03ca4756c1620f318fb189214388d8 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270376.1 LN:1136 M5:59e8fc80b78d62325082334b43dffdba AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270337.1 LN:1121 M5:16fc8d71a2662a6cfec7bdeec3d810c6 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270335.1 LN:1048 M5:eb27188639503b524d2659a23b8262ea AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270378.1 LN:1048 M5:fc13bda7dbd914c92fb7e49489d1350f AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270379.1 LN:1045 M5:3218bef25946cd95de585dfc7750f63b AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270329.1 LN:1040 M5:f023f927ae84c5cc48dc4dce11ba90f2 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270419.1 LN:1029 M5:58455e7a788f0dc82034d1fb109f6f5c AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270336.1 LN:1026 M5:69ad2d85d870c8b0269434581e86e30e AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270312.1 LN:998 M5:26499f2fe4c65621fd8f4ecafbad31d7 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270539.1 LN:993 M5:19e3a982e67eafef39c5a3e4163f1e17 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270385.1 LN:990 M5:112a8b1df94ef0498a0bfe2d2ea5cc23 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270423.1 LN:981 M5:bdf5a85c001731dccfb150db2bfe58ac AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270392.1 LN:971 M5:59b3ca8de65fb171683f8a06d3b4bf0d AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:KI270394.1 LN:970 M5:d5171e863a3d8f832f0559235987b1e5 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:CTRL00018_chrom LN:530 M5:134ad321330757fd6ce74969aa89f18f AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:CTRL00022_chrom LN:530 M5:136d044ab21cd25ed9487540dfca9037 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:CTRL00080_chrom LN:530 M5:0043cc5c9091b0c16666f837c6eaebe0 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:CTRL00087_chrom LN:530 M5:96bbb1465f9518b279761cf0e2a835ba AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:CTRL00096_chrom LN:530 M5:3fb0c8ab850845bb5ed5383599c5bf90 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:CTRL00196_chrom LN:530 M5:a4655b945dc2cc9c8f861f86b645a9e2 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:CTRL00275_chrom LN:530 M5:6a18feb048f08283028e9de1d6d08d97 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:CTRL00320_chrom LN:530 M5:ac7f97c9b87ab6538151d2edf686f4c9 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:CTRL00405_chrom LN:530 M5:883817de81453eb173e1461dd4da2dca AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:CTRL00484_chrom LN:530 M5:4db4a4c45f0bdfa90b370723a737ea57 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:CTRL00493_chrom LN:530 M5:f244c79ed53c99db4d98a6026a583f6b AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:CTRL00545_chrom LN:530 M5:ead97231927dda881c38122c206cc097 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:CTRL00616_chrom LN:530 M5:239d11e1d86ad0c45a1376d37ffd01e4 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:CTRL00637_chrom LN:530 M5:09907ec8e986f2b04491170d96e9eb8c AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:CTRL00640_chrom LN:530 M5:e980a34324be88ff952698eda5d49d0a AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:CTRL00673_chrom LN:530 M5:8e15a1b97cfe84d2c7d1d256cccb16c7 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:CTRL00688_chrom LN:530 M5:7a4090531c6f438d13a4abf55bde6207 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:CTRL00689_chrom LN:530 M5:11129f7051f0d9fe8598b9dbce0e2f08 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:CTRL00696_chrom LN:530 M5:6c65420f1d64533662cd9e1e8396bf89 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:CTRL00698_chrom LN:530 M5:8e8a35d1cd9ba5d099a188917d4e378a AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Essential_library_DHODH_1_chrom LN:530 M5:b41f135866c6ce79d408510eaa40fcb9 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Essential_library_DHODH_2_chrom LN:530 M5:f028bb706b7ff8698f5ee129b700f6fd AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Essential_library_DHODH_3_chrom LN:530 M5:fca6d91023dacf959e9bf0e33018fcf4 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Essential_library_MVD_1_chrom LN:530 M5:d52d61e87198903a8ece1831201914ba AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Essential_library_MVD_2_chrom LN:530 M5:e90f483c2f75dc33fecbfff8a4bf8853 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Essential_library_MVD_3_chrom LN:530 M5:bfdb845667d97a9c6c0e5b84271527d3 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Essential_library_TUBB_1_chrom LN:530 M5:02f469a4e5fd4d06b0de3555d6158fb2 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Essential_library_TUBB_2_chrom LN:530 M5:d6478fb8443232aa0ba2f2597bf6e2fc AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Essential_library_TUBB_3_chrom LN:530 M5:bede9010761621f4193ff74fbb3b67d1 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_BACH2_1_chrom LN:530 M5:18c3f94f2d738872ff74b4789bacacae AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_BACH2_2_chrom LN:530 M5:e57037b85cb1784d1fa44bd722212771 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_BACH2_3_chrom LN:530 M5:8917c02629d1f9febcec6affee600c75 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_DOK2_1_chrom LN:530 M5:079797a1120598175f0276fc6b0e92de AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_DOK2_2_chrom LN:530 M5:2db8218ad99ce0ba358c6b4de79407f7 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_DOK2_3_chrom LN:530 M5:2ae7be4b70afa4613e10272f8397637e AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_EGR1_1_chrom LN:530 M5:275e4b064720b2ac7d4de9c34e992d4d AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_EGR1_2_chrom LN:530 M5:7ba61b4f54e38ee1367ff5b32480a107 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_EGR1_3_chrom LN:530 M5:721b35114c4fbb60ead9fcc94091bf9d AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_EGR2_1_chrom LN:530 M5:0185b8f9ae6294d05bae94d909cc7687 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_EGR2_2_chrom LN:530 M5:07ddcb605d5577952530fcc8e23fee3c AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_EGR2_3_chrom LN:530 M5:4b9802a66752f5bf2f5a9d690b1c03b8 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_EGR3_1_chrom LN:530 M5:582a44921e830312040ad4cdd5a97610 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_EGR3_2_chrom LN:530 M5:aa6542ce370d7e5645ab813026e2ea10 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_EGR3_3_chrom LN:530 M5:a32a5877c5e554b2925d45b8e8a22fcb AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_EGR4_1_chrom LN:530 M5:5edce71161bbd0ee9507614a5c606672 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_EGR4_2_chrom LN:530 M5:a20facd66b9873845700e9e85db7a856 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_EGR4_3_chrom LN:530 M5:229014ed527a687a1311b361fccf608e AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_ETS1_1_chrom LN:530 M5:84ffea70041539e293a3c077536fdc8b AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_ETS1_2_chrom LN:530 M5:b138d85c8f384b3aa6f941ce62d361c4 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_ETS1_3_chrom LN:530 M5:e9effce2b8bed33a830b8d8f5c3d3056 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_FOS_1_chrom LN:530 M5:c7d9e6d3e1e18808cf92defa37e347cc AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_FOS_2_chrom LN:530 M5:3aa1e4ee40dd54c0003b1746484a3b3d AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_FOS_3_chrom LN:530 M5:5b960533ccb9ce9e1c3cb9843ebc79ea AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_GATA3_1_chrom LN:530 M5:a9c1339cde01ba760c921ab3bff138a7 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_GATA3_2_chrom LN:530 M5:25796781564c2c2948c5cf027f6d7a06 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_GATA3_3_chrom LN:530 M5:5a1ee817232e8d410eb7b75d443ce28a AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_JUN_1_chrom LN:530 M5:f1f0a4918bfb51dc04d1887cb818eb02 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_JUN_2_chrom LN:530 M5:a67b5279a84496aad97da6678944c5cf AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_JUN_3_chrom LN:530 M5:fba47ac2a469fccacc6114a28522c7dd AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_JUNB_1_chrom LN:530 M5:998bd8ba53509c056554bc10dce22e99 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_JUNB_2_chrom LN:530 M5:34bc1777c11fbcacf7876304d9c75d67 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_JUNB_3_chrom LN:530 M5:5bf78de2e3a5e223666fa426eddcd512 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_JUND_1_chrom LN:530 M5:051d0cfdf78e8a66a2299f4460b96100 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_JUND_2_chrom LN:530 M5:ba2657c8ac4a539269cff344e717d30f AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_JUND_3_chrom LN:530 M5:a33b0db5d6b8d21bea9d99400c963b37 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_LAT_1_chrom LN:530 M5:fa2120190976964ee703fb90614e1f68 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_LAT_2_chrom LN:530 M5:d8affb04564766b8d4352d5021a4309f AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_LAT_3_chrom LN:530 M5:9b90145c058c390c14e3a8ffe0ace21a AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_LCK_1_chrom LN:530 M5:817d06b48872a70a8ea0022ef98a052b AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_LCK_2_chrom LN:530 M5:ef103989718aa95421b2485198bf4b23 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_LCK_3_chrom LN:530 M5:f092867d49d891550e847bf8bb4abaac AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_NFAT5_1_chrom LN:530 M5:5aa9f1311d324448202d0b33349e58fe AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_NFAT5_2_chrom LN:530 M5:fcfdbb1c71482787c788cadf74288b1f AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_NFAT5_3_chrom LN:530 M5:a6758c3602cf02c2c9ff6118364ebab1 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_NFATC1_1_chrom LN:530 M5:982b66f26c3eb582eacb7a1bb23a4a7a AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_NFATC1_2_chrom LN:530 M5:a12b7a752e4db01b642aa6abfadc5afe AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_NFATC1_3_chrom LN:530 M5:021772ebf9f64a3b8e933f93ece4b36e AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_NFATC2_1_chrom LN:530 M5:f96d3ef61ab8e9176dd3cb0a42b335f3 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_NFATC2_2_chrom LN:530 M5:5a66fdf78dad4c7ac5e8e6461a74f428 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_NFATC2_3_chrom LN:530 M5:e69c5203dde414ca605fe36c30903489 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_NFATC3_1_chrom LN:530 M5:45552f7fd8369e17ef4dfbe3a2cea02c AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_NFATC3_2_chrom LN:530 M5:bf84fbec38f92d4e1608c2d929c4590d AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_NFATC3_3_chrom LN:530 M5:7e288697c67b43eb49bcbb93bc4ae268 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_NFKB1_1_chrom LN:530 M5:7a0a167d80d63fafcad6b42691191d23 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_NFKB1_2_chrom LN:530 M5:90a431bb2c81c1599c513b318fe58b05 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_NFKB1_3_chrom LN:530 M5:509eaa19ac70edd00addbab240f1853c AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_NFKB2_1_chrom LN:530 M5:7612ef994f185d0f1d08d148075ea942 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_NFKB2_2_chrom LN:530 M5:d7f5bdf067284e6513dd9126d8f83b8e AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_NFKB2_3_chrom LN:530 M5:19ce45d678a22f65115fadbfbd1a0483 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_NR4A1_1_chrom LN:530 M5:2959d5c9b46168f10758d0b439c87a50 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_NR4A1_2_chrom LN:530 M5:d517fd34b4a1ba3dbb00fc9d2022cd5a AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_NR4A1_3_chrom LN:530 M5:1acc1d2c5f1de39f7b4d6e1e29e29fc2 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_PTPN11_1_chrom LN:530 M5:8eb762494e7327b2b2e644a694164398 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_PTPN11_2_chrom LN:530 M5:9083a88868cbe575aa27a5025e9ef6f0 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_PTPN11_3_chrom LN:530 M5:c94e91df84ad93043954f980c7a14df0 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_PTPN6_1_chrom LN:530 M5:00c3f4f1fee803aae319bf74d010e7a1 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_PTPN6_2_chrom LN:530 M5:4383f12a3f02f6c38649497c0433cc50 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_PTPN6_3_chrom LN:530 M5:812d80aa501781f4ff5731cbd41a31f8 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_REL_1_chrom LN:530 M5:2a663501414d5ebffd3a54de5704912d AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_REL_2_chrom LN:530 M5:c945e519e608b415bf23f87b4a972950 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_REL_3_chrom LN:530 M5:6765e986a44342b074089c60507a5020 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_RELA_1_chrom LN:530 M5:a30c9d1c368c11fc837c7ee4e0818981 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_RELA_2_chrom LN:530 M5:68eb36a70ccaf7b090ca054b90911269 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_RELA_3_chrom LN:530 M5:9e5fb3c99d66c47c057a6c2348a25ba8 AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homo_sapiens.GRCh38.dna.primary_assembly.spiked.fa SP:human @SQ SN:Tcrlibrary_RELB_1_chrom LN:530 M5:f17d557ebe35f73e06fee6ef2172a26e AS:hg38_spiked UR:file:/scratch/lab_bock/shared/projects/crop-seq/spiked_genomes/hg38_spiked/Homosapiens.GRCh38.dna.primary

yysheep2020 commented 4 years ago

Hi, I would like to ask when I will use "cellranger count" function, did you make the ref genome by yourself. More specifically, do we need to add sgRNA sequence into the human reference genome?

afrendeiro commented 4 years ago

Hi @yysheep2020, for the CROP-seq paper we didn't use neither 10X data or cellranger. You can however run cellranger on CROP-seq data produced with the 10X platform by making a genome reference that includes gRNA sequences for example.