extend KOcall strategy beyond singlets

[sreichl]

support multiplets in a meaningful way alphabetically ordered gene names as KO type in snake_case e.g., KOA_KOB_KOC

• add column nKOcall (similar to Seurat nomenclature -> check again) describing number of KO genes assigned:
  • Negative (no KO): 0
  • Singlet: 1
  • Multiplet: X
• KOcall snakecase of all gene names:
  • Negative: NA
  • Singlet: KOA
  • Multiplet (alphabetically): KOA_KOB_KOC
• add column ngRNA describing number of guides assigned:
  • Negative: 0
  • Singlet: 1
  • Multiplet: X
• gRNAcall snakecase of all guide calls:
  • Negative: NA
  • Singlet: guide-1
  • Multiplet (alphabetically): guide-1_guide-300

note: gRNA multiplet can be a KO singlet! e.g., gRNAcall: geneX-1_geneX-2 -> KOcall: geneX

[sreichl]

Include logic

• If different gRNAs from same KO -> Single Ko call -> YES, because its a mix of the same phenotype

• If NT + KO -> KO -> NO, because its a mix of two different phenotypes Requires config for name of non targeting KO

Note: KO multiplets could also indicate droplet multiplets