search

epruesse / SINA

SINA - Reference based multiple sequence alignment
https://sina.readthedocs.io
GNU General Public License v3.0
41 stars 4 forks source link

difficulties using SINA with my own MSA #110

Closed korneel-emweb closed 3 months ago

korneel-emweb commented 4 months ago

I'm trying to use SINA with an MSA I created, it's a reduced version of the SILVA database. As mentioned in the documentation I tried running sina -i reference.fasta --prealigned -o reference.arb. When I run SINA with this database I get some errors:

No HELIX filter found in ARB file. Disabling secondary structure features.
loadKey failed: sequence 'EU214627.1.1520' not found
...

The result still seems sort of correct ... I'm still wondering if this has any bad effect on the result? What is the HELIX filter that is missing? And in general, would you recommend using SINA with my own MSA?

epruesse commented 3 months ago

Yeah, the bug is in the error message. I'm getting it myself. It's on the fix list. You are correct, other than spamming your log with red error lines, it doesn't cause any trouble.

korneel-emweb commented 3 months ago

OK thanks for the reply! That's reassuring.