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estepi / ASpli

Analysis of alternative splicing using RNAseq
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AsDiscover Error Message #10

Open diegogarcia101 opened 4 years ago

diegogarcia101 commented 4 years ago

Hello!

I have been trying out your package on a set of bam files covering a relatively small region that should contain a fair number of aberrant splicing events. I have been following the vignette closely and have successfully derived a "counts" object from which I can extract gene, bin, and junction info. The counts, features, bam, and target objects all appear to be working correctly. However, when I run:

as <- AsDiscover( counts, targets, features, bam, readLength=150L,threshold = 5)

I get the following error message:

Error in aggregate.data.frame(mf[1L], mf[-1L], FUN = FUN, ...) : no rows to aggregate

I am going to look at a genomic region that is a bit larger to see if that works for me but in the meantime, if you can offer any advice it would be much appreciated.

Thanks very much in advance!

Jon

CP1085 commented 4 years ago

Hi,

I am doing pretty much the exact same thing as Jon and get the same error message. Do you have any idea of how to fix the error? I can provide more details if necessary.

Thanks! CP