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estepi / ASpli

Analysis of alternative splicing using RNAseq
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The analysis of a single bam file #12

Open OlgaVT opened 4 years ago

OlgaVT commented 4 years ago

Hi,

We've been trying to analyze our data sets with ASpli. Interestingly, if we input only one Bam file, the error occured:

Error in colnames<-(*tmp*, value = as.character(row.names(targets))) : attempt to set 'colnames' on an object with less than two dimensions Calls: AsDiscover ... .filterJunctionBySample -> .extractCountColumns -> colnames<- -> colnames<-

Does it mean, that ASpli requires replicates and I overlooked it in the manual?

Thank you!

estepi commented 4 years ago

Dear Olva, thanks for posting it. Yes, as you said, ASpli is not prepared to proccess single samples. You need replicates (at least 1) and more than 1 condition. I hope it helps Estefi

OlgaVT commented 4 years ago

Thank you!