Hi I am trying to run ASpli to predict AS events for 1 RNA-seq sample. The code seems to output the following error for jCounts and I have had trouble figuring out the reason. I was wondering if you could help.
> asd= jCounts(counts = gbcounts,threshold= 5, features = features,minReadLength = readlength,libType="PE")
Junctions PJU completed
Error in (function (cl, name, valueClass) :
assignment of an object of class "standardGeneric" is not valid for @'junctionsPIR' in an object of class "ASpliAS"; is(value, "data.frame") is not TRUE
Hi I am trying to run ASpli to predict AS events for 1 RNA-seq sample. The code seems to output the following error for jCounts and I have had trouble figuring out the reason. I was wondering if you could help.
> asd= jCounts(counts = gbcounts,threshold= 5, features = features,minReadLength = readlength,libType="PE") Junctions PJU completed Error in (function (cl, name, valueClass) : assignment of an object of class "standardGeneric" is not valid for @'junctionsPIR' in an object of class "ASpliAS"; is(value, "data.frame") is not TRUE
Can you suggest a solution? Thanks Arko