Open IsabelGam opened 1 year ago
Hello! @estepi, any idea about this issue? Thanks a lot!
Hi both!
We can ignore/include "terminal" exons in our script. I need some info from your side:
thanks a lot for using ASpli, looking forward for your info
pd: can you copy-paste the issue in this repo please: https://github.com/chernolab/ASpli/issues
Hello,
I recently started to use ASpli to quantify the alternative splicing levels of my gene. My gene has 3 isoforms which only differ from each other by one exon.
The function binGenome() can detect the exon skipping from the second form. However, it does not detect the splicing event generated by an alternative exon 1. For now, I modified the GTF file to allow an overlap of the alternative starting exon, so that binGenome() detects it as an alt5'ss event. I wonder if there is a solution to this problem since I want to quantify alternative splicing levels for other genes, which also have an alternative starting exon (or ending exon).
Thanks