I am working with CNV's in a large Whole Genome sequencing file and using a flat reference generated without normal inputs. I am not sure if cnvkit is the right tool for this task.
Is there any "self normalization" happening on the sample input that can give me good results with a flat reference?
Any wisdom on CNV calling in this situation is welcome as I am new and not familiar with this practice.
Best Regards
I am working with CNV's in a large Whole Genome sequencing file and using a flat reference generated without normal inputs. I am not sure if cnvkit is the right tool for this task.
Is there any "self normalization" happening on the sample input that can give me good results with a flat reference?
Any wisdom on CNV calling in this situation is welcome as I am new and not familiar with this practice. Best Regards