Open enguyen117 opened 1 month ago
Hi @enguyen117 From the log information, there was something wrong with the reference that you used to index:
_[2024-10-18 11:56:10.689] [puff::index::jointLog] [error] In FixFasta, two references with the same name but different sequences: :516332sim4. We require that all input records have a unique name up to the first whitespace (or user-provided separator) character.
You have two transcripts with the same names in the reference, please fix their names. Also, when you run Scasa for annotation not from Human, please follow the instruction and do not use the docker version.
Nghia
Hi! I've used scasa before and published work on it. I am trying to use scasa with drosophila data. I used the drosophila new annotation you guys did here: https://github.com/eudoraleer/scasa/wiki/How-to-run-Scasa-for-a-new-annotation. I am using this reference genome: dmel-all-aligned-r6.46. I also am using the Docker version of scasa. But I keep running into this error. Scasa works fine when I do the sample case with 200 cells. Any help would be great!