NASI IPSC my 下

[evaleiyuhang]

split 1个12w 到1个6w：【split】：coat plate（remove，+E8 1ml），cell（remove，+PBS-，+EDTA 500ul 3min-，+E8 1ml，collect cells to coat plate 1个6w）

[evaleiyuhang]

feed，4个6w：【feed】remove，+pbs-，+3ml E8

[evaleiyuhang]

feed，4个6w：【feed】remove left little，+3ml E8

[evaleiyuhang]

feed，4个6w：【feed】remove，+3ml e8

[evaleiyuhang]

feed，4个6w：【feed】remove，+3ml e8

[evaleiyuhang]

infection ，dead

[evaleiyuhang]

收到新的ipsc

[evaleiyuhang]

feed

[evaleiyuhang]

"split 1：3，1:6 17:30-18:00🍎

Split 1 x 6w to coat vitronect 2 x 6w plate,1:6(just 1/3) Split 1 x 6w to float 3 x 6w plate,1:3

Split 1 x 6w to coat vitronect 2 x 6w plate,1:6(just 1/3)

1. Coat plate: 2 x 6w, 2ml PBS+ 20ul vitroncte /well, just add 2 x 6w, RT, wait 30min?
2. Coat plate, Remove vitronce and PBS, +2ml E8
3. 1 x 6w ipsc, Remove 95% medium,
4. Wash with 1ml PBS,-
5. • EDTA, 4min,-
6. +E8 1ml, split 1/3 cells to coat plate.
7. Add 0.5ml E8 with 1/3 cells to each coat plate well
8. 显微镜观察，恒温箱保存

Split 1 x 6w to float 3 x 6w plate,1:3

1. 1 x 6w ipsc, Remove 95% medium,
2. Wash with 1ml PBS,-
3. • EDTA, 3min,-
4. +E8 1ml, split all cells to coat plate.
5. Add 0.3ml E8 with all cells to each coat plate well 显微镜观察，恒温箱保存

Cells are fed by removing 95% of the medium from the wells using an aspirator pipette. Do not completely remove the medium; a thin film of medium should cover the cell layer to avoid drying out the cells. Aseptically add 2ml of fresh medium per 1 well of a 6-well plate by gently adding to the side of the well. Incubate cells at 37°C / 5 % CO2."

[evaleiyuhang]

"inflection again 16:30-17：00🥥"