Exomiser is a great tool! However, I have tried to make a custom vcf with < 5000 variants for a given sample.
the columns of the VCF are CHROM, POS, ID, REF, ALT and the other QUAL, FILTER, INFO, FORMAT kept empty [.], SAMPLE name comes after FORMAT column. the file is saved in vcf format (tab delimited).
The tool seems to run smoothly on linux cluster with no errors but I got this:
Annotating variant records, trimming sequences and normalising positions...
Processed 4500 variant records into 0 single allele variants!
Loaded 0 variants - 0 passed variant filters
Any suggestions or potential solutions for custom VCF files?
Your help is much needed to understand why I got this error?
Hi,
Exomiser is a great tool! However, I have tried to make a custom vcf with < 5000 variants for a given sample. the columns of the VCF are CHROM, POS, ID, REF, ALT and the other QUAL, FILTER, INFO, FORMAT kept empty [.], SAMPLE name comes after FORMAT column. the file is saved in vcf format (tab delimited). The tool seems to run smoothly on linux cluster with no errors but I got this: Annotating variant records, trimming sequences and normalising positions... Processed 4500 variant records into 0 single allele variants! Loaded 0 variants - 0 passed variant filters
Any suggestions or potential solutions for custom VCF files? Your help is much needed to understand why I got this error?
Thanks
N.