ConfigParser.NoSectionError: No section: 'names'

[Jami5on]

Hello Dr. Faircloth,

I am getting the following errors when attempting to clean my raw-data reads (see below). I have double checked the formatting of my file and wonder if the dash in the raw read files that I want to rename are what is mucking things up, or maybe another issue my tired eyes cannot see. I have attached my config file and pasted my SH below the error message. Additionally, I should mention I get the same error when working through the tutorial. I am using phyluce v 1.7.3 and accessing the university hpcc via Windows Powershell. My config and shell script are in my "scratch" with a folder for the raw reads.

illumiprocessor3.txt

Thank you in advance, Jamison

parallel file with error code:

2024-03-16 23:01:33,467 - illumiprocessor - INFO - ==================== Starting illumiprocessor =================== 2024-03-16 23:01:33,469 - illumiprocessor - INFO - Version: 2.10 2024-03-16 23:01:33,469 - illumiprocessor - INFO - Argument --config: illumiprocessor3.conf 2024-03-16 23:01:33,469 - illumiprocessor - INFO - Argument --cores: 4 2024-03-16 23:01:33,469 - illumiprocessor - INFO - Argument --input: /scratch/jr4253/raw_fastq/raw_fastq 2024-03-16 23:01:33,470 - illumiprocessor - INFO - Argument --log_path: None 2024-03-16 23:01:33,470 - illumiprocessor - INFO - Argument --min_len: 40 2024-03-16 23:01:33,470 - illumiprocessor - INFO - Argument --no_merge: False 2024-03-16 23:01:33,470 - illumiprocessor - INFO - Argument --output: /scratch/jr4253/raw_fastq/clean_fastq 2024-03-16 23:01:33,470 - illumiprocessor - INFO - Argument --phred: phred33 2024-03-16 23:01:33,470 - illumiprocessor - INFO - Argument --r1_pattern: None 2024-03-16 23:01:33,471 - illumiprocessor - INFO - Argument --r2_pattern: None 2024-03-16 23:01:33,471 - illumiprocessor - INFO - Argument --se: False 2024-03-16 23:01:33,471 - illumiprocessor - INFO - Argument --trimmomatic: /home/jr4253/.conda/envs/phyluce-1.7.3/bin/trimmomatic 2024-03-16 23:01:33,471 - illumiprocessor - INFO - Argument --verbosity: INFO Traceback (most recent call last): File "/home/jr4253/.conda/envs/phyluce-1.7.3/lib/python3.6/configparser.py", line 846, in items d.update(self._sections[section]) KeyError: 'names'

During handling of the above exception, another exception occurred:

Traceback (most recent call last): File "/home/jr4253/.conda/envs/phyluce-1.7.3/bin/illumiprocessor", line 17, in sys.exit(main()) File "/home/jr4253/.conda/envs/phyluce-1.7.3/lib/python3.6/site-packages/illumiprocessor/cli/main.py", line 114, in main main(args) File "/home/jr4253/.conda/envs/phyluce-1.7.3/lib/python3.6/site-packages/illumiprocessor/main.py", line 33, in main for start_name, end_name in conf.items("names"): File "/home/jr4253/.conda/envs/phyluce-1.7.3/lib/python3.6/configparser.py", line 849, in items raise NoSectionError(section) configparser.NoSectionError: No section: 'names'

SH

!/bin/bash

SBATCH --job-name=UCE_clean # Job name

SBATCH --mail-type=BEGIN,END,FAIL # Mail events (NONE, BEGIN, END, FAIL, ALL)

SBATCH --mail-user=jr4253@nau.edu # Where to send mail

SBATCH --nodes=1-1 # Run a single task

SBATCH --ntasks-per-node=16 # Number of CPU cores per task

SBATCH --mem=80gb # Job memory request

SBATCH --time=06-00:00:00 # Time limit days-hrs:min:sec

SBATCH --output=parallel_%j.log # Standard output and error log

SBATCH --chdir=/scratch/jr4253

module load anaconda3

conda activate phyluce-1.7.3

cd /scratch/jr4253/raw_fastq

illumiprocessor \ --input raw_fastq/ \ --output clean_fastq \ --config illumiprocessor3.conf \ --cores 4

[brantfaircloth]

It looks like your script might be putting you into the wrong directory - the cd command is placing you in /scratch/jr4253/raw_fastq - do you have raw_fastq folder nested inside of that (so like /scratch/jr4253/raw_fastq/raw_fastq?

[Jami5on]

Good morning Dr. Faircloth,

Currently, I have my SH and config in my scratch (/scratch/jr4253) and my raw reads are in the raw reads folder inside of that directory (/scratch/jr4253/raw_fatsq) but I do not have another folder within raw_fastq. So perhaps that is the error (fingers crossed!) and changing my cd to /scratch/jr4253 will help? I'll try this shortly and get back to you.

Thank you for your quick reply and help, Jami5on

[Jami5on]

Hello Dr.,

I have since updated my SH file and noticed I had some raw reads not accounted for in my config file. Omitting those (they're not needed now) and updating my SH worked!

Thank you so much for your help! Jami5on