phyluce_probe_get_tiled_probes error

[solenopsis1840]

Hi, I'm trying to design UCE probes but get the following error trying to design the temporary bait set from my base taxon:

phyluce_probe_get_tiled_probes --input ferVir1+5.fasta --probe-prefix "uce-" --design mealybug-v1 --designer gotzek --tiling-density 3 --two-probes --overlap middle --masking 0.25 --remove-gc --output ferVir1+5.temp.probes Probes removed for masking (.) / low GC % (G) / ambiguous bases (N): Traceback (most recent call last): File "/Users/dgotzek/anaconda/envs/phyluce/bin/phyluce_probe_get_tiled_probes", line 375, in main() File "/Users/dgotzek/anaconda/envs/phyluce/bin/phyluce_probe_get_tiled_probes", line 262, in main global_chromo, global_chromo_positions = global_coords.split(':') ValueError: need more than 1 value to unpack

This happens using phyluce 1.5.0 on both my laptop (MacOSX 10.12.4 with conda 4.3.16 build 2.1.10 and python 2.7.13.final.0) and our LINUX cluster (linux-64 with miniconda2 4.3.11 and python 2.7.13.final.0).

Any help is greatly appreciated! Thanks, Dietrich

[brantfaircloth]

hi Dietrich, can you send me your ferVir1+5.fasta file (or a portion of it)?

-b

On 2 May 2017, at 9:09, solenopsis1840 wrote:

Hi, I'm trying to design UCE probes but get the following error trying to design the temporary bait set from my base taxon:

phyluce_probe_get_tiled_probes --input ferVir1+5.fasta
--probe-prefix "uce-" --design mealybug-v1 --designer gotzek --tiling-density 3 --two-probes --overlap middle --masking 0.25
--remove-gc --output ferVir1+5.temp.probes Probes removed for masking (.) / low GC % (G) / ambiguous bases (N): Traceback (most recent call last): File "/Users/dgotzek/anaconda/envs/phyluce/bin/phyluce_probe_get_tiled_probes", line 375, in main() File "/Users/dgotzek/anaconda/envs/phyluce/bin/phyluce_probe_get_tiled_probes", line 262, in main global_chromo, global_chromo_positions = global_coords.split(':') ValueError: need more than 1 value to unpack

This happens using phyluce 1.5.0 on both my laptop (MacOSX 10.12.4 with conda 4.3.16 build 2.1.10 and python 2.7.13.final.0) and our LINUX cluster (linux-64 with miniconda2 4.3.11 and python 2.7.13.final.0).

Any help is greatly appreciated! Thanks, Dietrich

-- You are receiving this because you are subscribed to this thread. Reply to this email directly or view it on GitHub: https://github.com/faircloth-lab/phyluce/issues/66

[solenopsis1840]

Hi Brant, Thanks for the quick reply! I just sent you a dropbox invite. The file is in the bed folder. Cheers, Dietrich

[ClearloveMiao]

@solenopsis1840 Hi! How to fix this problem? ValueError: need more than 1 value to unpack Thanks a lot!