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fanglab / nanodisco

nanodisco: a toolbox for discovering and exploiting multiple types of DNA methylation from individual bacteria and microbiomes using nanopore sequencing.
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R10.4.1 support ? #83

Open DelphIONe opened 8 months ago

DelphIONe commented 8 months ago

Any news on this long-awaited update? Any difficulties? And, more generally, is Nanodisco being maintained?

Thanks for your answer,

touala commented 8 months ago

Hello @DelphIONe,

Thank you for your interest in nanodisco.

Yes, we are still working on this tool. Last summer, we assembled a diverse set of prokaryote samples for training and testing. The pore update to R10 is not trivial for method uplift as raw signal properties changed and the preprocessing pipeline had to be modified too. We will update the github as soon as we have a usable version.

Best,

Alan

DelphIONe commented 8 months ago

Thanks, is a great news :-)

DiWang-cau commented 4 months ago

Hi, I'm currently running a set of multiplex data using R10.4.1 and SQK-NBD114.24, However, the latest updated guppy has removed --fast5_out option. Is there any other way to demultiplex the data that can be moved to nanodisco? Thanks a million, Di