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fenghuibao / dSort-Seq

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Questions regarding the outputs of the codes #2

Closed jingliu-ut closed 5 days ago

jingliu-ut commented 1 week ago

I ran your example files and got the lamb.pickle, mu.pickle, and sigma.pickle. According to my understanding of your paper, you would get the estimated mean of each individual variant from Equation 1 in your methods section. image

Is my understanding correct? If so, could you provide the code for this part?

Thanks!

fenghuibao commented 5 days ago

Hi Jingliu,

The equation you mentioned is the log-scaled fluorescence distribution. To calculate the mean and SD you need to use Eq. 11 and Eq. 12 (see Obtaining expression characteristics from cytometry data through LGMM fitting in Method).

Best, Huibao

jingliu-ut commented 5 days ago

Thank you for responding, Huibao! Just to clarify things, to get the estimated Mean and SD for each variant (Fig 2I & J), I would use the outputs from your script (lamb.pickle, mu.pickle, and sigma.pickle) and plug them into equations 11 and 12?

fenghuibao commented 5 days ago

Yes, exactly!

Best, Huibao

jingliu-ut commented 5 days ago

Thanks!