fgcz / prolfqua

Differential Expression Analysis tool box R lang package for omics data
https://pubs.acs.org/doi/pdf/10.1021/acs.jproteome.2c00441
MIT License
40 stars 7 forks source link

Example run scripts for MSFragger and MaxQuant. #15

Closed wolski closed 4 years ago

wolski commented 4 years ago

Example run script.R where LFQService processes MQanalysis and MSStats file output.

wolski commented 4 years ago

Before running the scripts download package and build install it. Than set the working directory to LFQService/inst/run_scripts

Afterwards you can either source the files with source("lfq_mixed_model_inference.R") or alternatively open it in your editor and execute it line by line.

Example of MQ ouput analysis with a factorial design can be found here: https://github.com/wolski/LFQService/blob/master/inst/run_scripts/lfq_mixed_model_inference.R

Protein intensity level analysis using an msstats.csv file can be found here: https://github.com/wolski/LFQService/blob/master/inst/run_scripts/emulate_msstats_ProtL.R

Peptide level analysis using an msstats.csv file can be found here: https://github.com/wolski/LFQService/blob/master/inst/run_scripts/emulate_msstats_mixed.R