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fhalab / evSeq

Computational tools for extremely low-cost, massively parallel amplicon-based sequencing of every variant in protein mutant libraries.
https://fhalab.github.io/evSeq/
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Reads with Length Longer than Refseq Thrown Away #32

Open brucejwittmann opened 3 years ago

brucejwittmann commented 3 years ago

evSeq discards reads with insertions and deletions, and a read longer than the refseq has (relative to the refseq) a very large insertion. These reads are thus all thrown away. Conditional should be added to only use a portion of the read up to the refseq size.