fiji / Colocalisation_Analysis

Fiji's plugin for colocalization analysis
http://imagej.net/Coloc_2
GNU General Public License v3.0
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Black Image Instead of 2D Intensity Histogram #74

Open ScientistRachel opened 2 years ago

ScientistRachel commented 2 years ago

When running Coloc 2, the 2D Intensity Histogram, as well as any other images in the dropdown menu (channel 1 max projection, etc.) show up as black images. If I run my cursor over the images, I see varied pixel values on the Fiji toolbar, but those variations are not visible:

coloc 2 - new version of Fiji

I am comparing this behavior to an older installation of Fiji. If I use the same images in both the newer version (image above), I get the following images in the older installation:

coloc 2 - old version of Fiji

We would like to be able to run Coloc 2 with some of the updates that have been applied in the newer version of Fiji. Are there any options to make the information in the scatterplot visible with the most recent updates to Fiji? Thank you for any suggestions!

chalkie666 commented 2 years ago

@ScientistRachel this appears to be a new bug somewhere in Fiji, unless thr code for coloc2 changed recently. Others have also seen this. @ctrueden any clues what changes might have lead to this?

ctrueden commented 2 years ago

@chalkie666 I'm on vacation this week, but will try to investigate after I return on July 11th.

ScientistRachel commented 1 year ago

In case it helps with this bug, it turns out you can sometimes see part of the scatter plot. On some data sets, if I turn the Log checkbox off in the bottom right corner you can see the brightest part of the peak as a small region of the scatter plot. So perhaps it is problem with the colormap scaling? Also, the ability to export the data as a list is intact, so the histogram is being generated, just not displayed.

imagesc-bot commented 1 year ago

This issue has been mentioned on Image.sc Forum. There might be relevant details there:

https://forum.image.sc/t/2d-intensity-histogram-for-fiji-coloc-2-wont-show/74616/2