fiji / SPIM_Registration

Deprecated, instead check out: https://github.com/preibischLab/multiview-reconstruction & https://github.com/PreibischLab/BigStitcher
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blending parameters persists for next deconvolution even when 'adjust_blending_parameters' not set #122

Open RichardMyers opened 7 years ago

RichardMyers commented 7 years ago

I'm running the following macro and find that afterwards I get the same image result (ie. shows adjusted blending parameters), even when adjust_blending_parameters is not set.

run("Fuse/Deconvolve Dataset", "select_xml=C:\Users\Richard\Downloads\\beads_02micro_1.sld_capture_5.xml process_angle=[All angles] process_channel=[Single channel (Select from List)] process_illumination=[Single illumination (Select from List)] process_timepoint=[Single Timepoint (Select from List)] processing_channel=[channel 488a] processing_illumination=[illumination capture_5] processing_timepoint=[Timepoint 0] type_of_image_fusion=[Multi-view deconvolution] bounding_box=[Use pre-defined Bounding Box] fused_image=[Display using ImageJ] bounding_box_title=dim_175.38x972.80x175.38_px_offset_0.00x0.00x0.00_px imglib2_container=[CellImg (large images)] imglib2_container_ffts=[PlanarImg (large images, easy to display)] type_of_iteration=[Independent (slow, very precise)] image_weights=[Virtual weights (less memory, slower)] osem_acceleration=[1 (balanced)] number_of_iterations=1 adjust_blending_parameters use_tikhonov_regularization tikhonov_parameter=0.0060 compute=[Entire image at once] compute_on=[CPU (Java)] psf_estimation=[Provide file with PSF] psf_display=[Do not show PSFs] psffile(angle=path_a,=[C:\Users\Richard\Downloads\\beads_02micro_1.sld transfomed PSF of viewsetup 0.tif] psffile(angle=path_b,=[C:\Users\Richard\Downloads\\beads_02micro_1.sld transfomed PSF of viewsetup 1.tif] boundary_pixels_x=0 boundary_pixels_y=0 boundary_pixels_z=0 blending_range_x=0 blending_range_y=0 blending_range_z=0");

Is this expected behavior? How should I reset the blending parameters to the default behavior?

Thanks!

Result images 1) macro before adjusting blending

image

2) same macro but adjusting blending image

3) same macro before adjusting blending (but looks like image 2 not image 1) image