fiji / Stitching

Fiji's Stitching plugins reconstruct big images from tiled input images.
http://imagej.net/Stitching
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Stitching large dataset issue #33

Open ctrueden opened 9 years ago

ctrueden commented 9 years ago

From an ImageJ mailing list thread:

Doing some stitching of a "large" dataset I encountered a « bug ».

Stitching parameters

  • Issue : Weird pixel attribution
  • DATASET :
    • Channel : 1
    • Each Z-stack: 2048x2048x92 (around 750Mb / stack)
    • Total Positions : 112 (16x7)
    • Eachtiled-slice is around 700Mb,(total around 85 Go)
  • Downscaled dataset
    • Channel : 1
    • Each Z stack: 512x512x23 (around 11Mb / stack)
    • Total Positions : 112 (16x7)
    • Each tiled-slice is around 30Mb, (total around 1.25 Go)
  • Computer :
    • CPU IntelXeonx64, 16 Cores, 2.6Ghz,RAM 128Gb
    • GPU : Quadro6000

If the stitching of the original dataset is done with computing the overlap. We observed a weird pixel attribution. a bug ?

1

If the stitching of the original dataset is done without computing the overlap. We don’t observed anymore the bug.

2

Using a downscaled version of the data set, we don’t observed a bug if the stitching is done with or without computing overlap

3