I have 4 ome tiff files, and each single tif file contains a Z-stack Tile of 2 channels(Red channel and green channel.), and I want to stitch them using "unknown position".
In this case, was the first channel selected to do the stitching, and the second channel follows the same transformation, or the two channels are bind together forming a 2xZ(height) Z-stack and use this kind of four doubleZ-stack for stitching?
Thanks.
I have 4 ome tiff files, and each single tif file contains a Z-stack Tile of 2 channels(Red channel and green channel.), and I want to stitch them using "unknown position".
In this case, was the first channel selected to do the stitching, and the second channel follows the same transformation, or the two channels are bind together forming a 2xZ(height) Z-stack and use this kind of four doubleZ-stack for stitching? Thanks.
Below is the log:
Stitching internal version: 1.1 D:\QMDownload\7\7\1composite.tif D:\QMDownload\7\7\2composite.tif D:\QMDownload\7\7\3composi.tif D:\QMDownload\7\7\4compo.tif Found 4 files (we ignore hidden and .txt files). Loading: D:\QMDownload\7\7\1composite.tif ... 115x161x160px, channels=2, timepoints=1 (7450 ms) Loading: D:\QMDownload\7\7\2composite.tif ... 115x161x160px, channels=2, timepoints=1 (7388 ms) Loading: D:\QMDownload\7\7\3composi.tif ... 115x161x160px, channels=2, timepoints=1 (8317 ms) Loading: D:\QMDownload\7\7\4compo.tif ... 115x161x160px, channels=2, timepoints=1 (6878 ms) 1composite.tif[1] <- 2composite.tif[1]: (0.0, 0.0, 0.0) correlation (R)=0.99658525 (91328 ms) 1composite.tif[1] <- 3composi.tif[1]: (0.0, 0.0, 0.0) correlation (R)=0.99000984 (84785 ms) 1composite.tif[1] <- 4compo.tif[1]: (0.0, 0.0, 0.0) correlation (R)=0.9924897 (82371 ms) 2composite.tif[1] <- 3composi.tif[1]: (0.0, 0.0, 0.0) correlation (R)=0.99284816 (82912 ms) 2composite.tif[1] <- 4compo.tif[1]: (0.0, 0.0, 0.0) correlation (R)=0.9883379 (80999 ms) 3composi.tif[1] <- 4compo.tif[1]: (0.0, 0.0, 0.0) correlation (R)=0.9964178 (85912 ms) Finished registration process (508534 ms). 1composite.tif: 1,3 0.0 2composite.tif: 1,3 0.0 3composi.tif: 1,3 0.0 4compo.tif: 1,3 0.0 Fuse & Write to disk (into directory 'D:\QMDownload\7\7') ... Finished fusion (4425 ms) Finished ... (566826 ms)