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fiji / Stitching

Fiji's Stitching plugins reconstruct big images from tiled input images.
http://imagej.net/Stitching
GNU General Public License v2.0
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Stitching .tif files ends in a all white image #74

Closed mrwhy-orig closed 1 year ago

mrwhy-orig commented 1 year ago

I'm currently trying to stitch several tif files (1pager). After fusion the image blinks for a moment and the goes all white. The saved images are all white as well. I tried opening my files with FIJI which is working fine.

Edit: I'm new to ImageJ /FIJI... I found that while stitching there is a 4 channel added to the resulting tiff, which is black and white. So my ROIs are white the rest is black. If I delete the 4th channel the image is looking great. Is this a wanted behavior?

imagejan commented 1 year ago

Can you please provide more detail on exactly how you're stitching? Ideally some recorded macro code (Plugins › Macros › Record...) to reproduce the issue?

mrwhy-orig commented 1 year ago

Thanks, I discovered the problem. It was lack of knowledge and not a bug in the plugin.