Resizing image process is unstable

[nhuhoa]

Dear Ignacio Arganda-Carreras,

Thanks for very nice work. My work need to process big volume images and have tested several image registration plugins. And your plugins runs very well, fast execution time, multi-threads, good output. However, when I tested your plugin, I have noticed a small issue that the outputs are not stable. I think the problem maybe is at resizing image step cause I did not have time to go through the code. For my tests:

• First I use dapi images as references. Based on that I get the transformation maps (.xml files) Size of raw dapi images: 13850 x 20544, registered images: 14080 x 20715 pixels
• Then I apply the transformation maps .xml to process different channels (markers).
  • First test: marker images: 13850 x 20544, registered images: 14079 x 20715 pixels. The results are not what I expect, the width of registered images is less 1px.
  • Second test: marker images: 13850 x 20544, registered images: 14080 x 20715 pixels. The results are as expected. The size of marker images are the same as reference images.

So I note the issue here for you. Thanks a lot, Hoa Tran

[iarganda]

Hello @nhuhoa and thanks for reporting! Could you upload somewhere the XML files of a couple of your images so I can try to reproduce the error? Thanks a lot!

[nhuhoa]

Dear Ignacio Arganda-Carreras, Thanks for very quick reply. I send you the .xml file in the following link. Can you help me to look at it? I send 3 .xml files that I used, all produces same error. https://drive.google.com/open?id=1ECmZAkOpuH8dkVGdTZcdmduRQyRgXN7E If in case you still can not detect any error, I will try to prepare some small images, test it and send you the images + xml files. Thanks a lot, Hoa Tran

[iarganda]

Thanks a lot, @nhuhoa . I have tried with your .xml files and I got the expected image sizes. Can you check if the pixel size is different on the images that gave you the problem?

[nhuhoa]

Dear Ignacio Arganda-Carreras, I think you figure out the correct answer. I tested 3 images and find the mapping: reference image & round 1 image, reference image & round 2 image. I rescaled the image of round 2 with the ratio of 0.97 before doing registration process. I guess this is the reason of unstable result. I will test it again without rescaling image. Thanks a lot for your support. Best, Hoa Tran

[iarganda]

OK, great! I'm glad we figured it out.