fjug / MoMA

MoMA - the MotherMachine Analyzer
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[release issue] Check moma robustness to changes in phase contrast images #25

Open julou opened 8 years ago

julou commented 8 years ago

It would be useful to check how well MoMA works when the phase contrast acquisition is changed: although the image intensity is normalized, a change in the dynamic range will affect thresholding / segment generation...

Obviously linked to #20 which would provide a general solution to this as well (by allowing users to customize moma to their input images)

erikvannimwegen commented 8 years ago

More explicit explanation: All data that has currently been processed is coming from one particular microscopic setup and illumination setting. Consequently, it is unclear to what extent MoMA is robust to changes in the precise dynamic range and quality of the phase contrast images. We can provide some example data where some parameters are varied. We propose that Florian then checks to what extent MoMA is already robust to such variations, or if it isn't, whether there would be simple fixes to make it more robust. If not, then we need to document what type of images have been tested.

fjug commented 8 years ago

My suggestion would be to make MoMA available to other groups and help maybe two selected partners to get acceptable results on their datasets. How does this sound?

erikvannimwegen commented 8 years ago

Well, for sure this will start to happen when we put the paper on the archive and make the code available. We just thought that it may make sense to do just a minimal robustness check for changing some illumination settings etc. If it works, great. If not, we may want to include some specification in the manuscript of the conditions that MoMA has been shown to perform satisfactorily on. By the time the paper is coming back from review somewhere you should then have had some experience with datasets from others.