Cyanoseq database in Qiime2

[Listen-Lii]

Thank you for developing the Cyanoseq database, which is very helpful for aquatic microbial research. I want to use Cyanoseq database on my 16S rRNA gene amplicon sequencing data. I downloaded CyanoSeq_1.2_SILVA138.1_QIIME2_classifier.qza and found it is a trained database. So, I run these codes in Qiime2:

qiime feature-classifier classify-sklearn \ --i-reads zotus.fa.qza \ --i-classifier CyanoSeq_1.2_SILVA138.1_QIIME2_classifier.qza \ --o-classification taxonomy.qza But there is an error: Plugin error from feature-classifier: 0.

Here are messages in debug log file:

Traceback (most recent call last): File "/share/soft/miniconda3/envs/qiime2202208/lib/python3.8/site-packages/q2cli/commands.py", line 339, in call results = action(**arguments) File "", line 2, in classify_sklearn File "/share/soft/miniconda3/envs/qiime2202208/lib/python3.8/site-packages/qiime2/sdk/action.py", line 223, in bound_callable callable_args[name] = artifact._view( File "/share/soft/miniconda3/envs/qiime2202208/lib/python3.8/site-packages/qiime2/sdk/result.py", line 373, in _view result = transformation(self._archiver.data_dir) File "/share/soft/miniconda3/envs/qiime2202208/lib/python3.8/site-packages/qiime2/core/transform.py", line 70, in transformation new_view = transformer(view) File "/share/soft/miniconda3/envs/qiime2202208/lib/python3.8/site-packages/q2_feature_classifier/_taxonomic_classifier.py", line 72, in _1 pipeline = joblib.load(os.path.join(dirname, 'sklearn_pipeline.pkl')) File "/share/soft/miniconda3/envs/qiime2202208/lib/python3.8/site-packages/joblib/numpy_pickle.py", line 587, in load obj = _unpickle(fobj, filename, mmap_mode) File "/share/soft/miniconda3/envs/qiime2202208/lib/python3.8/site-packages/joblib/numpy_pickle.py", line 506, in _unpickle obj = unpickler.load() File "/share/soft/miniconda3/envs/qiime2202208/lib/python3.8/pickle.py", line 1212, in load dispatchkey[0] KeyError: 0

Qiime2 works well when using other reference database with the same data. So, I think there may be some problems with the file CyanoSeq_1.2_SILVA138.1_QIIME2_classifier.qza. Can you fix this?

Thank you very much for your reply and suggestions.

[Apatzingan1]

Maybe the compatibility whit the Qiime version, actually working in qiime2-2023.5 and it runs smoothly

[Listen-Lii]

Thank you very much, I used qiime2-2023.5 and now it works!