flimfit / FLIMfit

State of the art fluorescence lifetime imaging analysis software
http://flimfit.org
GNU General Public License v2.0
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Error when loading .tiff image from Lambert Instruments software #358

Open bzorila opened 4 years ago

bzorila commented 4 years ago

Error using circshift Invalid shift type: must be a real finite integer vector.

Error in flim_data_series/compute_tr_data (line 92)

Error in flim_data_series/init_dataset (line 123)

Error in flim_data_series/load_multiple (line 119)

Error in flim_data_series/load_single (line 104)

Error in flim_data_series_controller/load_single (line 168)

Error in front_end_menu_controller/menu_file_load_single_callback (line 656)

Error in front_end_menu_controller>@(varargin)obj.menu_file_load_single_callback(varargin{:})

Error in EC (line 7)

Error in front_end_menu_controller>@(x,y)EC(fcn) (line 263)

seanwarren commented 4 years ago

Hello, thanks for the report. If you can send me an example file I should be able to resolve this

bzorila commented 4 years ago

Original message

Hello, thanks for the reply! I manage to open the .tiff stack images from our device. Anyway, as we are at the very first beginning with the software and hardware from Lambert Instruments, I think there is a problem with the settings for the camera. I attached the images from this morning, which I deal to open in FlimFit. In these images there are some cells treated with Phalloidin.. If you could analyze these pics and extract the lifetimes it will be very helpful for me.

Thanks in advance! Bogdan

New message It seems that the images is to big to be transmitted as attachment. I send you link from wetransfer website: https://wetransfer.com/downloads/1febbf85178dfa9ab686b457f56fcdb220190910100151/0115c9129b1563c40af2fcbfc404e96d20190910100151/b81d41

On Tue, Sep 10, 2019 at 12:55 PM bzorila . bzorila@gmail.com wrote:

Hello, thanks for the reply! I manage to open the .tiff stack images from our device. Anyway, as we are at the very first beginning with the software and hardware from Lambert Instruments, I think there is a problem with the settings for the camera. I attached the images from this morning, which I deal to open in FlimFit. In these images there are some cells treated with Phalloidin.. If you could analyze these pics and extract the lifetimes it will be very helpful for me.

Thanks in advance! Bogdan

On Tue, Sep 10, 2019 at 12:52 PM bzorila . bzorila@gmail.com wrote:

Hello, thanks for the reply! I manage to open the .tiff stack images from our device. Anyway, as we are at the very first beginning with the software and hardware from Lambert Instruments, I think there is a problem with the settings for the camera. I attached the images from this morning, which I deal to open in FlimFit. In these images there are some cells treated with Phalloidin.. If you could analyze these pics and extract the lifetimes it will be very helpful for me.

Thanks in advance! Bogdan

On Mon, Sep 9, 2019 at 9:54 PM Sean Warren notifications@github.com wrote:

Hello, thanks for the report. If you can send me an example file I should be able to resolve this

— You are receiving this because you authored the thread. Reply to this email directly, view it on GitHub https://github.com/flimfit/FLIMfit/issues/358?email_source=notifications&email_token=ANEJLZJCRVYHJULBBCDWUSTQI2LVZA5CNFSM4IUZ3RQ2YY3PNVWWK3TUL52HS4DFVREXG43VMVBW63LNMVXHJKTDN5WW2ZLOORPWSZGOD6IVE5Y#issuecomment-529617527, or mute the thread https://github.com/notifications/unsubscribe-auth/ANEJLZJWYLQCC5A4FXX3GLDQI2LVZANCNFSM4IUZ3RQQ .

-- Phys. Bogdan Zorila Horia Hulubei National Institute of Physics and Nuclear Engineering - IFIN HH Department of Life and Environmental Sciences Magurele, Ilfov Romania +40 21 404 6149 +40 728 925 696

-- Phys. Bogdan Zorila Horia Hulubei National Institute of Physics and Nuclear Engineering - IFIN HH Department of Life and Environmental Sciences Magurele, Ilfov Romania +40 21 404 6149 +40 728 925 696

-- Phys. Bogdan Zorila Horia Hulubei National Institute of Physics and Nuclear Engineering - IFIN HH Department of Life and Environmental Sciences Magurele, Ilfov Romania +40 21 404 6149 +40 728 925 696